SRR3548675_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548675_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	303202
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4255	1.4033548591368132	No Hit
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT	4141	1.3657561625582946	No Hit
AATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT	2497	0.8235433803207104	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC	2491	0.8215645015534198	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGT	2319	0.7648366435577602	No Hit
AAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT	1617	0.5333078277847771	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTT	1117	0.36840126384390603	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1016	0.3350901379278501	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	789	0.26022255789869464	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	678	0.2236133007038212	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTT	674	0.22229404819229426	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT	645	0.21272946748372373	No Hit
AACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTG	631	0.20811208369337933	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT	519	0.1711730133706242	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	492	0.16226805891781715	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	451	0.14874572067466574	No Hit
AATGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTC	414	0.13654263494304125	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTAT	375	0.12367992295565333	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT	361	0.11906253916530894	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	359	0.11840291290954544	No Hit
AACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT	317	0.10455076153851228	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGTA	20	7.0250634E-4	45.0	45
CGTGGGA	40	6.7866495E-9	45.0	4
CGAACAG	20	7.0250634E-4	45.0	1
GATCGTG	20	7.0250634E-4	45.0	29
AGGAGTA	20	7.0250634E-4	45.0	35
CTATCGG	20	7.0250634E-4	45.0	2
GCAACTC	20	7.0250634E-4	45.0	21
TTCACGG	40	6.7866495E-9	45.0	2
CAACCCG	40	6.7866495E-9	45.0	1
CCTACCG	20	7.0250634E-4	45.0	1
TATGCTC	20	7.0250634E-4	45.0	24
TCGATTG	20	7.0250634E-4	45.0	1
ACGGGTG	20	7.0250634E-4	45.0	5
CGTTCCG	20	7.0250634E-4	45.0	1
CCGTCAA	20	7.0250634E-4	45.0	13
ATCGTGA	20	7.0250634E-4	45.0	30
TAGCACG	20	7.0250634E-4	45.0	1
GTTACGG	20	7.0250634E-4	45.0	2
GGCCAAT	20	7.0250634E-4	45.0	22
TCGTGAG	20	7.0250634E-4	45.0	31

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content