##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548675_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	303202
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.999812006517107	31.0	31.0	33.0	28.0	34.0
2	31.244744427807206	31.0	31.0	34.0	28.0	34.0
3	31.47699553433025	31.0	31.0	34.0	30.0	34.0
4	35.44262900640497	37.0	35.0	37.0	33.0	37.0
5	34.728534112571815	35.0	35.0	37.0	32.0	37.0
6	34.80324008416831	35.0	35.0	37.0	32.0	37.0
7	34.77805555372326	35.0	35.0	37.0	32.0	37.0
8	35.16782211199135	36.0	35.0	37.0	32.0	37.0
9	36.63209015771664	39.0	35.0	39.0	32.0	39.0
10	35.96037625081629	37.0	35.0	39.0	31.0	39.0
11	36.20074735654778	37.0	35.0	39.0	32.0	39.0
12	35.987269213263765	37.0	35.0	39.0	32.0	39.0
13	35.70015039478631	37.0	35.0	39.0	30.0	39.0
14	36.630447688339785	38.0	35.0	40.0	31.0	41.0
15	36.7158165183607	38.0	35.0	40.0	31.0	41.0
16	36.527315123251164	38.0	35.0	40.0	31.0	41.0
17	36.46878978370855	38.0	35.0	40.0	31.0	41.0
18	36.30907118026926	38.0	35.0	40.0	31.0	41.0
19	36.35691057446851	38.0	35.0	40.0	31.0	41.0
20	36.23416402266476	38.0	34.0	40.0	30.0	41.0
21	36.32152822210935	38.0	34.0	40.0	30.0	41.0
22	36.254975231034095	38.0	34.0	40.0	30.0	41.0
23	36.14637766241648	38.0	34.0	40.0	30.0	41.0
24	35.75186179510689	38.0	34.0	40.0	29.0	41.0
25	35.79335888285697	38.0	34.0	40.0	29.0	41.0
26	35.74229721439832	38.0	34.0	40.0	29.0	41.0
27	35.57532931840819	38.0	34.0	40.0	28.0	41.0
28	35.39626057875608	38.0	34.0	40.0	27.0	41.0
29	35.10253230519588	37.0	34.0	40.0	26.0	41.0
30	35.06936959518737	37.0	34.0	40.0	26.0	41.0
31	34.98027717495267	37.0	34.0	40.0	26.0	41.0
32	34.92272478413731	37.0	34.0	40.0	25.0	41.0
33	34.839948285301546	37.0	34.0	40.0	25.0	41.0
34	34.86478651196232	37.0	34.0	40.0	25.0	41.0
35	34.71993588432794	37.0	33.0	40.0	25.0	41.0
36	34.80724071740951	37.0	33.0	40.0	25.0	41.0
37	34.71960936933134	37.0	33.0	40.0	25.0	41.0
38	34.67952388176859	37.0	33.0	40.0	25.0	41.0
39	34.623894301488775	37.0	33.0	40.0	24.0	41.0
40	34.341465425689805	37.0	33.0	40.0	24.0	41.0
41	34.457671783167655	37.0	33.0	40.0	24.0	41.0
42	34.3669072103746	37.0	33.0	40.0	24.0	41.0
43	34.38470062862382	37.0	33.0	40.0	24.0	41.0
44	34.207630556526674	37.0	33.0	40.0	23.0	41.0
45	34.07675081298936	37.0	33.0	40.0	23.0	41.0
46	33.992836458862406	37.0	33.0	40.0	23.0	41.0
47	33.93006972249523	37.0	33.0	40.0	23.0	41.0
48	33.95678788398494	37.0	33.0	40.0	23.0	41.0
49	33.76472450709428	36.0	33.0	40.0	22.0	41.0
50	33.67983720424008	36.0	33.0	40.0	22.0	41.0
51	32.3357827454964	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	6.0
12	9.0
13	4.0
14	18.0
15	24.0
16	74.0
17	155.0
18	318.0
19	621.0
20	1133.0
21	1749.0
22	2363.0
23	2870.0
24	3172.0
25	3394.0
26	3637.0
27	4324.0
28	5098.0
29	6345.0
30	8278.0
31	10750.0
32	14144.0
33	19333.0
34	29583.0
35	32930.0
36	34783.0
37	45077.0
38	49135.0
39	23871.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.18753834077612	36.967434251752955	17.735700951840688	26.10932645563024
2	19.541098013865344	38.04889149807719	19.17467562878873	23.23533485926874
3	18.333322339562404	35.581229675265995	22.24952342003021	23.835924565141394
4	19.4569956662555	33.83486916313217	18.7868153903998	27.921319780212535
5	15.940198283652482	37.30021569778563	19.228764981761334	27.53082103680055
6	19.193145163950106	36.41367801003951	21.753814288823953	22.63936253718643
7	71.88244140869783	12.222214893041603	9.253896742105923	6.641446956154643
8	74.13110731459554	9.199807389133317	7.170797026404839	9.498288269866293
9	69.47546520141688	12.23870554943569	12.624257095929448	5.661572153217986
10	32.46119748550471	39.70818134445023	12.491672218520986	15.338948951524067
11	23.61824790073944	28.618544732554536	31.024531500451847	16.73867586625418
12	24.955640134299905	24.565141390887923	34.33123792059419	16.14798055421798
13	22.798002651697548	33.57893417589594	25.385056826801932	18.23800634560458
14	13.24595484198653	37.24249840040633	32.326963542456845	17.184583215150294
15	12.186265262102493	32.16502529666691	35.49152050448216	20.157188936748437
16	13.515082354338032	33.2309813259807	32.13567192828543	21.118264391395837
17	14.00089709170784	30.3009214978793	28.165711307972902	27.53247010243996
18	19.257128910759164	28.98661618327056	26.656156621658166	25.10009828431211
19	19.584303533617852	33.73460597225612	28.044669890040304	18.636420604085725
20	19.87519871240955	32.798596315327735	25.864275301614104	21.461929670648612
21	18.40225328328969	33.55848576196727	26.136371132116544	21.902889822626502
22	15.562892065355769	33.602680721103425	26.852395432747805	23.982031780793005
23	17.69876188151793	31.731321033502418	23.183884011319186	27.386033073660464
24	14.00716354113759	33.44898780351053	27.30786736235249	25.235981292999387
25	14.788161027961557	33.644896801472285	27.12218257135507	24.44475959921109
26	19.46985837824289	34.04693900436013	24.056899360822158	22.426303256574826
27	14.562898661618325	33.016272979729685	27.167696783002746	25.253131575649235
28	19.222828345459465	31.978021253157962	29.3091734223389	19.489976979043675
29	20.196436698966366	30.58785891913642	26.537753708748625	22.677950673148594
30	20.62123600767805	31.388645193633284	27.136364535853986	20.853754262834677
31	22.67894011253224	33.38599349608512	22.292069313526955	21.642997077855686
32	22.078350406659585	29.08457068225144	24.002810007849554	24.834268903239426
33	23.41937058462675	28.94176159787864	26.77950673148594	20.85936108600867
34	18.30825654184339	28.302583756043827	25.913747270796367	27.475412431316414
35	20.53383552878939	28.078970455340002	29.069069465240993	22.318124550629616
36	22.719507127261693	25.08162874915073	32.387649157987084	19.81121496560049
37	18.58068218547371	27.487945330175922	29.830607977519936	24.10076450683043
38	21.565161179675595	26.299298817290122	30.821036800548807	21.314503202485472
39	22.254140803820555	24.08955086048245	28.061490359562274	25.59481797613472
40	24.543043911319845	26.78511355465993	24.108020395643827	24.563822138376395
41	18.958318216898306	23.32471421692469	31.12215618630484	26.594811379872162
42	24.658478506078456	26.287425544686382	26.83854328137677	22.21555266785839
43	20.923015019689846	24.847791241482575	30.062796419548683	24.166397319278897
44	20.347820924664084	24.60076120869915	28.389984234932484	26.661433631704277
45	23.43058423097473	26.873833286060115	27.09975527865911	22.59582720430604
46	19.124544033350706	25.30062466606421	31.760014775628132	23.814816524956957
47	21.852098600932713	24.851748999017158	27.008067229107986	26.288085170942143
48	21.412127888338468	21.107380558175738	34.335525491256654	23.14496606222914
49	21.707310637792627	20.588584508017757	31.799262537846058	25.904842316343558
50	19.36069023291403	22.31350716683927	28.7329898879295	29.5928127123172
51	18.78714520352768	22.455326811828417	33.73526559851188	25.022262386132017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4903.0
1	3863.5
2	2824.0
3	1926.5
4	1029.0
5	1156.0
6	1283.0
7	1211.0
8	1139.0
9	1159.5
10	1180.0
11	1169.5
12	1159.0
13	1241.5
14	1324.0
15	1371.0
16	1418.0
17	1366.0
18	1314.0
19	1240.5
20	1167.0
21	1226.0
22	1285.0
23	1338.5
24	1392.0
25	1560.5
26	2183.0
27	2637.0
28	2759.0
29	2881.0
30	3106.5
31	3332.0
32	4237.0
33	5142.0
34	5749.0
35	6356.0
36	7123.5
37	7891.0
38	8604.0
39	9317.0
40	10950.0
41	12583.0
42	16336.5
43	20090.0
44	27043.5
45	33997.0
46	34328.5
47	34660.0
48	36300.5
49	37941.0
50	34865.5
51	31790.0
52	27402.5
53	23015.0
54	18944.0
55	14873.0
56	12612.5
57	10352.0
58	8997.0
59	7642.0
60	6501.0
61	5360.0
62	4696.5
63	4033.0
64	3282.5
65	2532.0
66	2016.0
67	1500.0
68	1078.5
69	657.0
70	640.0
71	623.0
72	491.0
73	359.0
74	247.5
75	138.0
76	140.0
77	112.0
78	84.0
79	83.0
80	82.0
81	53.0
82	24.0
83	18.5
84	13.0
85	9.0
86	5.0
87	6.5
88	8.0
89	4.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	303202.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.96250175645177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.18173971560294	33.646263936513485
2	9.157302124762005	7.685266129893703
3	3.1453738787932584	3.959632707406789
4	1.5056470134724118	2.527228617897298
5	0.8799559710098532	1.8462576989550596
6	0.5997880775605565	1.5101164954880213
7	0.4446286211255302	1.3060410506464157
8	0.3729951135628666	1.2521446486423777
9	0.31082480896133213	1.173868793278982
>10	3.1223971833366813	27.6846765898141
>50	0.2281993595088531	6.17805233837916
>100	0.040131611499832784	3.5073902692818506
>500	0.004721366058803856	1.2996693748722807
>1k	0.006295154745071809	6.423391348930507
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4255	1.4033548591368132	No Hit
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT	4141	1.3657561625582946	No Hit
AATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT	2497	0.8235433803207104	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC	2491	0.8215645015534198	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGT	2319	0.7648366435577602	No Hit
AAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT	1617	0.5333078277847771	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTT	1117	0.36840126384390603	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1016	0.3350901379278501	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	789	0.26022255789869464	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	678	0.2236133007038212	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTT	674	0.22229404819229426	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT	645	0.21272946748372373	No Hit
AACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTG	631	0.20811208369337933	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT	519	0.1711730133706242	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	492	0.16226805891781715	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	451	0.14874572067466574	No Hit
AATGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTC	414	0.13654263494304125	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTAT	375	0.12367992295565333	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT	361	0.11906253916530894	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	359	0.11840291290954544	No Hit
AACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT	317	0.10455076153851228	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2981312788174223E-4	0.0	0.0	1.5999234833543314	0.0
2	3.2981312788174223E-4	0.0	0.0	2.187650477239596	0.0
3	3.2981312788174223E-4	0.0	0.0	2.9214846867764726	0.0
4	3.2981312788174223E-4	0.0	0.0	6.512160210025	0.0
5	6.596262557634845E-4	0.0	0.0	7.177393288962474	0.0
6	6.596262557634845E-4	0.0	0.0	9.935620477437483	0.0
7	6.596262557634845E-4	0.0	0.0	12.050712066543097	0.0
8	6.596262557634845E-4	0.0	0.0	13.435597390518533	0.0
9	6.596262557634845E-4	0.0	0.0	15.901610147690318	0.0
10	6.596262557634845E-4	0.0	0.0	18.51999656994347	0.0
11	6.596262557634845E-4	0.0	0.0	21.73237643551164	0.0
12	6.596262557634845E-4	0.0	0.0	23.25281495504647	0.0
13	6.596262557634845E-4	0.0	0.0	23.94509271047025	0.0
14	6.596262557634845E-4	0.0	0.0	24.654850561671758	0.0
15	6.596262557634845E-4	0.0	0.0	25.142644177808855	0.0
16	6.596262557634845E-4	0.0	0.0	26.06216317834315	0.0
17	6.596262557634845E-4	0.0	0.0	27.19012407569871	0.0
18	6.596262557634845E-4	0.0	0.0	29.032790021174	0.0
19	6.596262557634845E-4	0.0	0.0	29.669329357985767	0.0
20	6.596262557634845E-4	0.0	0.0	30.477701334423916	0.0
21	6.596262557634845E-4	0.0	0.0	31.105665529910752	0.0
22	6.596262557634845E-4	0.0	0.0	31.776175618894335	0.0
23	6.596262557634845E-4	0.0	0.0	32.43778075342511	0.0
24	6.596262557634845E-4	0.0	0.0	32.85829249147433	0.0
25	6.596262557634845E-4	0.0	0.0	33.23922665417774	0.0
26	6.596262557634845E-4	0.0	0.0	33.57794473651229	0.0
27	6.596262557634845E-4	0.0	0.0	34.078930877764655	0.0
28	6.596262557634845E-4	0.0	0.0	34.52714691855595	0.0
29	6.596262557634845E-4	0.0	0.0	34.91599659632852	0.0
30	6.596262557634845E-4	0.0	0.0	35.57199490768531	0.0
31	6.596262557634845E-4	0.0	0.0	35.99580477701335	0.0
32	6.596262557634845E-4	0.0	0.0	36.350353889486215	0.0
33	6.596262557634845E-4	0.0	0.0	36.73491599659633	0.0
34	6.596262557634845E-4	0.0	0.0	37.13695819948417	0.0
35	6.596262557634845E-4	0.0	0.0	37.53405320545379	0.0
36	6.596262557634845E-4	0.0	0.0	37.890911009821835	0.0
37	6.596262557634845E-4	0.0	0.0	38.25700358177057	0.0
38	6.596262557634845E-4	0.0	0.0	38.60957381547615	0.0
39	6.596262557634845E-4	0.0	0.0	38.98786947315651	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGTA	20	7.0250634E-4	45.0	45
CGTGGGA	40	6.7866495E-9	45.0	4
CGAACAG	20	7.0250634E-4	45.0	1
GATCGTG	20	7.0250634E-4	45.0	29
AGGAGTA	20	7.0250634E-4	45.0	35
CTATCGG	20	7.0250634E-4	45.0	2
GCAACTC	20	7.0250634E-4	45.0	21
TTCACGG	40	6.7866495E-9	45.0	2
CAACCCG	40	6.7866495E-9	45.0	1
CCTACCG	20	7.0250634E-4	45.0	1
TATGCTC	20	7.0250634E-4	45.0	24
TCGATTG	20	7.0250634E-4	45.0	1
ACGGGTG	20	7.0250634E-4	45.0	5
CGTTCCG	20	7.0250634E-4	45.0	1
CCGTCAA	20	7.0250634E-4	45.0	13
ATCGTGA	20	7.0250634E-4	45.0	30
TAGCACG	20	7.0250634E-4	45.0	1
GTTACGG	20	7.0250634E-4	45.0	2
GGCCAAT	20	7.0250634E-4	45.0	22
TCGTGAG	20	7.0250634E-4	45.0	31
>>END_MODULE