FastQCFastQC Report
Sat 18 Jun 2016
SRR3548674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338042
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30500.9022547494098367No Hit
CTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTGCT29750.8800681572112341No Hit
AATCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCT24140.7141124475656871No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCC19780.5851343915844777No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGT17190.5085166931919702No Hit
AAACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCT11430.3381236651067027No Hit
AAAAACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTT9910.29315883825086825No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6800.20115843593399638No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5970.17660527390087624No Hit
AACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTG5510.1629974973524No Hit
AATGCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTC5340.15796853645405007No Hit
AAAAAACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCT5080.1502771844918679No Hit
AATGACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTT4950.14643150851077677No Hit
AATGATCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCT4550.13459865933818874No Hit
CCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTGC4250.12572402245874773No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3830.1132995308275303No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3550.10501653640671868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAA351.2085184E-745.0000049
CCATAGG351.2085184E-745.0000042
CGGACCG351.2085184E-745.0000041
AGCGTTC207.026214E-445.025
CCGGGTA950.045.05
ATTAGTA207.026214E-445.017
CCTACTC207.026214E-445.038
GCACGGT207.026214E-445.09
CGCGCCT453.8380676E-1045.017
CAATTGC207.026214E-445.021
TGCGGGT207.026214E-445.04
TCGGATG207.026214E-445.020
AATGCGG207.026214E-445.02
GTAGATA207.026214E-445.013
CGGATGT207.026214E-445.021
CTAAAAG207.026214E-445.01
AGTTGAC207.026214E-445.028
CACTACG207.026214E-445.01
GGTGCGT207.026214E-445.027
CGTACAG207.026214E-445.01