FastQCFastQC Report
Sat 18 Jun 2016
SRR3548673_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548673_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342901
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30800.898218436225033No Hit
CTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTGCT29630.864097800822978No Hit
AATCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCT25580.7459879090466345No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCC21980.6410013385787734No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGT17920.5225998174400192No Hit
AAACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCT11760.3429561301950126No Hit
AAAAACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTT9420.2747148593909029No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6830.19918285452652515No Hit
AACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTG6160.17964368724500657No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6010.1752692468088457No Hit
AATGACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTT5600.16331244295000596No Hit
AATGCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTC5510.16068777868830944No Hit
AAAAAACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCT5450.1589380025138451No Hit
AATGATCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCT4840.14114861140679089No Hit
CCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTGC4450.1297750662727726No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4210.12277596157491522No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3720.10848612281678968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCG302.1610304E-645.0000041
CCTAAGA302.1610304E-645.0000049
AGTACCC302.1610304E-645.00000415
TAGTCCG302.1610304E-645.0000041
TCGCGCA302.1610304E-645.00000434
CTAGACG207.0263556E-445.01
CTATGCA207.0263556E-445.020
CCGGGCC453.8380676E-1045.05
ATTAGTC207.0263556E-445.011
GCGACGA207.0263556E-445.013
ATCTACG253.8850623E-545.01
CCCTATA207.0263556E-445.011
TTTCGTG207.0263556E-445.029
CTATATC207.0263556E-445.013
CGTTCTA406.7902874E-945.027
CATTCGC207.0263556E-445.022
GGCCCTA207.0263556E-445.09
TACATTG253.8850623E-545.01
AATGCGG207.0263556E-445.02
GTAGCCG207.0263556E-445.043