##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548673_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	342901
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.093855077704642	31.0	31.0	33.0	28.0	34.0
2	31.35234076307739	31.0	31.0	34.0	30.0	34.0
3	31.574215298293094	31.0	31.0	34.0	30.0	34.0
4	35.49205747431474	37.0	35.0	37.0	33.0	37.0
5	34.818157427362415	35.0	35.0	37.0	32.0	37.0
6	34.91543623378176	35.0	35.0	37.0	32.0	37.0
7	34.91648318319282	35.0	35.0	37.0	32.0	37.0
8	35.290214960003034	37.0	35.0	37.0	33.0	37.0
9	36.779898571307754	39.0	37.0	39.0	32.0	39.0
10	36.175000947795425	38.0	35.0	39.0	32.0	39.0
11	36.3405472716615	38.0	35.0	39.0	32.0	39.0
12	36.00354912934054	37.0	35.0	39.0	32.0	39.0
13	35.73821890283201	37.0	35.0	39.0	30.0	39.0
14	36.71003584124864	38.0	35.0	40.0	31.0	41.0
15	36.78147336986477	38.0	35.0	40.0	32.0	41.0
16	36.61140679088133	38.0	35.0	40.0	31.0	41.0
17	36.53110372964792	38.0	35.0	40.0	31.0	41.0
18	36.393827956173936	38.0	35.0	40.0	31.0	41.0
19	36.46987322871616	38.0	35.0	40.0	31.0	41.0
20	36.338371716617914	38.0	35.0	40.0	30.0	41.0
21	36.46026695751835	38.0	35.0	40.0	31.0	41.0
22	36.41898098868187	38.0	35.0	40.0	30.0	41.0
23	36.32828717326575	38.0	35.0	40.0	31.0	41.0
24	35.93733759889881	38.0	34.0	40.0	30.0	41.0
25	36.052236651395006	38.0	34.0	40.0	30.0	41.0
26	35.960936246905085	38.0	34.0	40.0	30.0	41.0
27	35.81768498779531	38.0	34.0	40.0	29.0	41.0
28	35.605370063079434	38.0	34.0	40.0	28.0	41.0
29	35.254720750304024	38.0	34.0	40.0	27.0	41.0
30	35.338059673200135	38.0	34.0	40.0	27.0	41.0
31	35.16769563226704	38.0	34.0	40.0	27.0	41.0
32	35.16495431626038	38.0	34.0	40.0	27.0	41.0
33	35.05484965048221	37.0	34.0	40.0	26.0	41.0
34	34.993554991090726	37.0	34.0	40.0	26.0	41.0
35	34.836617566003014	37.0	33.0	40.0	25.0	41.0
36	34.91336858160227	37.0	34.0	40.0	26.0	41.0
37	34.76194295146413	37.0	33.0	40.0	25.0	41.0
38	34.63427928177521	37.0	33.0	40.0	25.0	41.0
39	34.63770009419628	37.0	33.0	40.0	25.0	41.0
40	34.439182154616056	37.0	33.0	40.0	24.0	41.0
41	34.51931607081927	37.0	33.0	40.0	24.0	41.0
42	34.423192116675075	37.0	33.0	40.0	24.0	41.0
43	34.443941545810596	37.0	33.0	40.0	24.0	41.0
44	34.35339354507569	37.0	33.0	40.0	24.0	41.0
45	34.147377814587884	36.0	33.0	40.0	23.0	41.0
46	34.04668402833471	36.0	33.0	40.0	23.0	41.0
47	33.974074149681684	36.0	33.0	40.0	23.0	41.0
48	33.998588513885935	36.0	33.0	39.0	23.0	41.0
49	33.81364883741955	36.0	33.0	39.0	23.0	41.0
50	33.741934260909126	36.0	33.0	39.0	23.0	41.0
51	32.36046555711415	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	6.0
12	12.0
13	18.0
14	32.0
15	54.0
16	109.0
17	200.0
18	357.0
19	723.0
20	1170.0
21	1667.0
22	2321.0
23	2798.0
24	3237.0
25	3470.0
26	3919.0
27	4696.0
28	5521.0
29	7120.0
30	9007.0
31	12072.0
32	16072.0
33	22529.0
34	34431.0
35	36618.0
36	38824.0
37	49009.0
38	55511.0
39	31391.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.410844529470605	34.10255438158536	19.53071003000866	24.95589105893538
2	23.329765734133176	34.5035447549001	18.674486221970774	23.49220328899595
3	19.964071262551	33.04918912455781	21.18337362679024	25.803365986100946
4	21.43300836101382	33.52279520911283	18.570666168952556	26.473530260920793
5	16.802808974018742	36.276651278357306	18.595163035395057	28.325376712228895
6	19.5100043452775	37.33001653538485	21.789379441879724	21.370599677457925
7	75.6661543710867	10.060338115082779	8.223948019982444	6.049559493848078
8	77.11992674270417	7.730219509421087	6.671313294507744	8.478540453367007
9	71.89276205085433	10.230649662730643	11.578852205155425	6.297736081259606
10	36.221241699499274	30.927585513019796	14.65029265006518	18.200880137415755
11	29.910090667568774	28.406449674979072	24.053589811636595	17.629869845815556
12	28.24226234394184	24.609727005753847	29.624585521768676	17.523425128535642
13	24.5948539082709	31.58083528481982	24.154493571030706	19.66981723587858
14	16.922085383244724	33.81646597706043	30.02907544743235	19.23237319226249
15	16.278167751041845	28.78352644057614	33.353358549552205	21.58494725882981
16	17.49921989145555	29.90950740884395	31.134642360331405	21.45663033936909
17	17.46334947987903	27.682042338750833	27.355125823488414	27.49948235788172
18	22.444670619216627	26.656965129877136	26.085954838276937	24.812409412629304
19	22.580278272737612	32.577916074902085	25.449327940134324	19.39247771222598
20	23.590774013490776	30.91504545043613	24.411710668676964	21.082469867396128
21	20.908950396761746	31.483139448412224	25.0381305391352	22.569779615690827
22	18.815051574652742	30.946249792214083	26.129990872000956	24.108707761132223
23	20.21866369593556	30.365032472929503	22.81183198649173	26.604471844643207
24	18.173466977349147	30.71411281973514	26.80044677618321	24.311973426732497
25	19.435347228500355	29.55430284542769	25.18656988460226	25.823780041469696
26	20.768093414717367	31.420147506131507	24.085377412139366	23.72638166701176
27	17.47909746544921	29.557802397776616	26.02646244834515	26.936637688429023
28	19.95648889912832	31.402066485662044	27.163816961746978	21.477627653462662
29	19.288657659207757	30.276668776119052	28.376995109375592	22.057678455297594
30	22.316645329118316	28.688455268430246	25.364463795672805	23.630435606778633
31	22.996725002260128	32.428310211985384	22.05592867912313	22.51903610663136
32	23.960560045027574	27.73657702952164	22.320436510829655	25.98242641462113
33	23.937812954759536	28.996124245773565	23.14253968346549	23.923523116001412
34	18.854713167940602	29.670371331667155	24.901939626889394	26.57297587350285
35	19.569205105846876	30.7456087908755	25.940723415796395	23.744462687481228
36	21.587571923091506	27.90251413673334	27.69691543623378	22.81299850394137
37	19.298864686892134	31.82405417307036	22.13321046016197	26.74387067987553
38	22.308479706970815	30.879466668222022	22.420465382136534	24.391588242670625
39	22.430964039183323	25.40354213023584	25.140492445341366	27.025001385239474
40	25.837486621502997	26.618178424676508	22.324227692540997	25.220107261279495
41	19.366814328333835	24.99292798796154	27.673001828516103	27.967255855188522
42	24.523113085117863	28.02995616810683	23.23819411433621	24.208736632439102
43	20.739805366563527	26.13786486478605	26.78265738507616	26.33967238357427
44	21.90748933365607	24.22623439418374	25.441745576711643	28.424530695448542
45	23.691677772884887	26.860230795477413	25.441745576711643	24.006345854926057
46	20.401515306167088	25.9652202822389	28.58142729242551	25.051837119168507
47	21.316939874774352	25.911560479555323	25.32742686664664	27.44407277902368
48	21.503874296079626	23.148955529438524	31.723150413676247	23.6240197608056
49	22.971353247730395	22.323644433816174	28.63683687128355	26.068165447169882
50	19.82846360903001	23.772750735635068	27.782654468782535	28.616131186552384
51	20.141090285534307	23.686428444361493	30.70915512057416	25.46332614953004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3669.0
1	2896.0
2	2123.0
3	1459.0
4	795.0
5	1027.0
6	1259.0
7	1183.0
8	1107.0
9	1050.5
10	994.0
11	1051.0
12	1108.0
13	1145.5
14	1183.0
15	1142.0
16	1101.0
17	1105.5
18	1110.0
19	1099.5
20	1089.0
21	1160.5
22	1232.0
23	1159.0
24	1086.0
25	1466.5
26	2051.0
27	2255.0
28	2902.5
29	3550.0
30	3832.0
31	4114.0
32	4772.5
33	5431.0
34	6164.0
35	6897.0
36	7347.0
37	7797.0
38	8052.0
39	8307.0
40	10293.0
41	12279.0
42	16042.0
43	19805.0
44	25522.0
45	31239.0
46	33272.0
47	35305.0
48	36030.0
49	36755.0
50	34536.5
51	32318.0
52	29318.5
53	26319.0
54	22829.5
55	19340.0
56	17156.5
57	14973.0
58	13954.5
59	12936.0
60	12137.0
61	11338.0
62	10037.5
63	8737.0
64	7516.5
65	6296.0
66	5651.0
67	5006.0
68	4250.0
69	3494.0
70	3154.5
71	2815.0
72	2547.0
73	2279.0
74	1820.5
75	1036.5
76	711.0
77	662.0
78	613.0
79	530.0
80	447.0
81	304.5
82	162.0
83	156.5
84	151.0
85	100.5
86	50.0
87	41.5
88	33.0
89	34.0
90	35.0
91	35.5
92	36.0
93	22.0
94	8.0
95	6.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	342901.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.732625159638125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.31307078347638	30.304229952923983
2	8.299073501904417	6.2629165923928936
3	2.888044942460608	3.2692055177416433
4	1.420010653891054	2.143229189038551
5	0.8611716517422378	1.6247133566648142
6	0.6331653051235206	1.4334593473388222
7	0.44858564946434326	1.184841991426162
8	0.3881232470241708	1.1715927196563736
9	0.33543651479391834	1.1391210249816897
>10	3.9764017005121626	34.167801191847715
>50	0.3873369234106499	9.180416188739887
>100	0.03950836618788629	2.7795261419023656
>500	0.005422716927749099	1.3147868951810746
>1k	0.004648043080927799	4.02415989016404
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3080	0.898218436225033	No Hit
CTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTGCT	2963	0.864097800822978	No Hit
AATCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCT	2558	0.7459879090466345	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCC	2198	0.6410013385787734	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGT	1792	0.5225998174400192	No Hit
AAACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCT	1176	0.3429561301950126	No Hit
AAAAACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTT	942	0.2747148593909029	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	683	0.19918285452652515	No Hit
AACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTG	616	0.17964368724500657	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	601	0.1752692468088457	No Hit
AATGACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTT	560	0.16331244295000596	No Hit
AATGCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTC	551	0.16068777868830944	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCT	545	0.1589380025138451	No Hit
AATGATCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCT	484	0.14114861140679089	No Hit
CCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTGC	445	0.1297750662727726	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	421	0.12277596157491522	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	372	0.10848612281678968	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.91629362410725E-4	0.0	0.0	1.0527819983027171	0.0
2	2.91629362410725E-4	0.0	0.0	1.4569802946039818	0.0
3	2.91629362410725E-4	0.0	0.0	2.0393641313382	0.0
4	2.91629362410725E-4	0.0	0.0	4.719437971892762	0.0
5	2.91629362410725E-4	0.0	0.0	5.286365452419211	0.0
6	2.91629362410725E-4	0.0	0.0	7.4152597980175035	0.0
7	2.91629362410725E-4	0.0	0.0	9.085712785906136	0.0
8	2.91629362410725E-4	0.0	0.0	10.272935920280197	0.0
9	2.91629362410725E-4	0.0	0.0	12.122449336689016	0.0
10	2.91629362410725E-4	0.0	0.0	14.22830496265686	0.0
11	2.91629362410725E-4	0.0	0.0	16.676241830732486	0.0
12	2.91629362410725E-4	0.0	0.0	17.737189451182704	0.0
13	2.91629362410725E-4	0.0	0.0	18.313157441943886	0.0
14	2.91629362410725E-4	0.0	0.0	18.832257707034977	0.0
15	2.91629362410725E-4	0.0	0.0	19.183087830015076	0.0
16	2.91629362410725E-4	0.0	0.0	19.824380797956263	0.0
17	2.91629362410725E-4	0.0	0.0	20.54820487545968	0.0
18	2.91629362410725E-4	0.0	0.0	21.79900321083928	0.0
19	2.91629362410725E-4	0.0	0.0	22.190953073919296	0.0
20	2.91629362410725E-4	0.0	0.0	22.663392641024668	0.0
21	2.91629362410725E-4	0.0	0.0	23.134957320042812	0.0
22	2.91629362410725E-4	0.0	0.0	23.586107943692202	0.0
23	2.91629362410725E-4	0.0	0.0	24.034633903079897	0.0
24	2.91629362410725E-4	0.0	0.0	24.367091376228124	0.0
25	2.91629362410725E-4	0.0	0.0	24.653763039477866	0.0
26	2.91629362410725E-4	0.0	0.0	24.933435598029753	0.0
27	2.91629362410725E-4	0.0	0.0	25.257144190305656	0.0
28	2.91629362410725E-4	0.0	0.0	25.52835949734763	0.0
29	5.8325872482145E-4	0.0	0.0	25.824363300194516	0.0
30	5.8325872482145E-4	0.0	0.0	26.267056672333997	0.0
31	5.8325872482145E-4	0.0	0.0	26.55722788793267	0.0
32	8.748880872321749E-4	0.0	0.0	26.854106578866787	0.0
33	8.748880872321749E-4	0.0	0.0	27.18510590520296	0.0
34	8.748880872321749E-4	0.0	0.0	27.525437371136274	0.0
35	8.748880872321749E-4	0.0	0.0	27.833689607204413	0.0
36	8.748880872321749E-4	0.0	0.0	28.104321655521563	0.0
37	8.748880872321749E-4	0.0	0.0	28.380494661724523	0.0
38	8.748880872321749E-4	0.0	0.0	28.660167220276406	0.0
39	8.748880872321749E-4	0.0	0.0	29.000790315572132	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACCG	30	2.1610304E-6	45.000004	1
CCTAAGA	30	2.1610304E-6	45.000004	9
AGTACCC	30	2.1610304E-6	45.000004	15
TAGTCCG	30	2.1610304E-6	45.000004	1
TCGCGCA	30	2.1610304E-6	45.000004	34
CTAGACG	20	7.0263556E-4	45.0	1
CTATGCA	20	7.0263556E-4	45.0	20
CCGGGCC	45	3.8380676E-10	45.0	5
ATTAGTC	20	7.0263556E-4	45.0	11
GCGACGA	20	7.0263556E-4	45.0	13
ATCTACG	25	3.8850623E-5	45.0	1
CCCTATA	20	7.0263556E-4	45.0	11
TTTCGTG	20	7.0263556E-4	45.0	29
CTATATC	20	7.0263556E-4	45.0	13
CGTTCTA	40	6.7902874E-9	45.0	27
CATTCGC	20	7.0263556E-4	45.0	22
GGCCCTA	20	7.0263556E-4	45.0	9
TACATTG	25	3.8850623E-5	45.0	1
AATGCGG	20	7.0263556E-4	45.0	2
GTAGCCG	20	7.0263556E-4	45.0	43
>>END_MODULE