##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548672_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	475286
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.937704876642695	31.0	31.0	33.0	28.0	34.0
2	31.232142331143773	31.0	31.0	34.0	28.0	34.0
3	31.401349924045732	31.0	31.0	34.0	30.0	34.0
4	35.38008062513939	37.0	35.0	37.0	33.0	37.0
5	34.6545448424738	35.0	35.0	37.0	32.0	37.0
6	34.80196134537941	35.0	35.0	37.0	32.0	37.0
7	34.76541282512003	35.0	35.0	37.0	32.0	37.0
8	35.22096379863913	37.0	35.0	37.0	33.0	37.0
9	36.67900800781003	39.0	35.0	39.0	32.0	39.0
10	35.99065404829934	37.0	35.0	39.0	31.0	39.0
11	36.266824606657885	37.0	35.0	39.0	32.0	39.0
12	35.952599066667226	37.0	35.0	39.0	31.0	39.0
13	35.69346877459046	37.0	35.0	39.0	30.0	39.0
14	36.634691533097964	38.0	35.0	40.0	31.0	41.0
15	36.71286341276621	38.0	35.0	40.0	31.0	41.0
16	36.44971659169426	38.0	35.0	40.0	31.0	41.0
17	36.40292371330104	38.0	35.0	40.0	31.0	41.0
18	36.27632415009068	38.0	35.0	40.0	30.0	41.0
19	36.2790109534049	38.0	34.0	40.0	30.0	41.0
20	36.294250198827655	38.0	34.0	40.0	30.0	41.0
21	36.50049233514137	38.0	35.0	40.0	31.0	41.0
22	36.43771960461701	38.0	35.0	40.0	31.0	41.0
23	36.392422667614866	38.0	35.0	40.0	31.0	41.0
24	35.92477581919097	38.0	34.0	40.0	30.0	41.0
25	36.14023977142184	38.0	34.0	40.0	30.0	41.0
26	36.10292329250178	38.0	35.0	40.0	30.0	41.0
27	36.039771842637904	38.0	34.0	40.0	30.0	41.0
28	35.955721397221886	38.0	34.0	40.0	30.0	41.0
29	35.5846942682932	38.0	34.0	40.0	29.0	41.0
30	35.687430305121545	38.0	34.0	40.0	29.0	41.0
31	35.524233829736204	38.0	34.0	40.0	28.0	41.0
32	35.54543790475629	38.0	34.0	40.0	29.0	41.0
33	35.44834268209036	38.0	34.0	40.0	28.0	41.0
34	35.29727364155477	38.0	34.0	40.0	27.0	41.0
35	35.209488602651874	38.0	34.0	40.0	27.0	41.0
36	35.33633643742925	38.0	34.0	40.0	27.0	41.0
37	35.202488606859866	38.0	34.0	40.0	27.0	41.0
38	35.15887065892957	38.0	34.0	40.0	27.0	41.0
39	35.01090501298166	37.0	34.0	40.0	26.0	41.0
40	34.743872110687036	37.0	33.0	40.0	25.0	41.0
41	34.8172026947985	37.0	33.0	40.0	26.0	41.0
42	34.84944433456908	37.0	33.0	40.0	26.0	41.0
43	34.97225880838064	37.0	34.0	40.0	27.0	41.0
44	34.926503200178416	37.0	34.0	40.0	26.0	41.0
45	34.73499955816077	37.0	33.0	40.0	26.0	41.0
46	34.62455868676965	37.0	33.0	40.0	25.0	41.0
47	34.53477485135266	37.0	33.0	40.0	25.0	41.0
48	34.58825633408095	37.0	33.0	40.0	26.0	41.0
49	34.520074228990545	37.0	33.0	40.0	26.0	41.0
50	34.44777881107375	37.0	33.0	40.0	26.0	41.0
51	33.046746590473944	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	8.0
11	4.0
12	13.0
13	20.0
14	25.0
15	67.0
16	95.0
17	210.0
18	342.0
19	695.0
20	1226.0
21	1808.0
22	2343.0
23	3023.0
24	3531.0
25	3953.0
26	4657.0
27	5602.0
28	7086.0
29	9360.0
30	12593.0
31	17259.0
32	22727.0
33	31798.0
34	47860.0
35	50947.0
36	56292.0
37	71487.0
38	78651.0
39	41599.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.99505981661568	39.55576221475069	19.02159962633025	23.42757834230337
2	19.980180354565462	38.62621663587819	18.637199496724076	22.756403512832275
3	17.863560045951278	30.59505224222889	27.631783810169036	23.909603901650794
4	24.68240175389134	31.10779614800352	18.335907222177806	25.873894875927338
5	15.926831423605996	38.87301540546113	18.808675197670453	26.39147797326242
6	19.92253085510619	34.0578094031804	25.309813459685326	20.709846282028085
7	72.59986618583338	12.226112277660189	8.858455750853171	6.31556578565327
8	74.59024671460973	5.753377966108827	6.586350113405402	13.07002520587604
9	72.7925922497191	8.77113990313201	11.8947328555859	6.541534991562975
10	35.59057073004465	35.3454551575262	14.065425869897282	14.998548242531864
11	25.396918907773426	23.654809946011454	30.918646877879848	20.029624268335276
12	26.024330613567408	22.646995703639494	34.8661647934086	16.462508889384498
13	23.639029973531724	30.568962687729073	25.616996923957362	20.175010414781834
14	14.921541976830794	31.366798096304123	35.34208876339719	18.36957116346789
15	14.549134626309213	29.694752212352142	31.669563168281833	24.086549993056813
16	17.182496433726218	29.716633774190697	32.84569711710423	20.255172674978855
17	16.699208476580417	27.948856057195037	28.780986605959356	26.570948860265187
18	20.085169771463917	27.60716705310066	27.202989357986564	25.104673817448862
19	19.32331270014265	32.30833645426122	30.75516636298986	17.61318448260626
20	22.200948481545847	32.670855022028846	25.501277125772692	19.62691937065262
21	18.631729106264437	30.827964636029677	27.655558968705158	22.884747289000728
22	17.97633424927307	31.23929591866792	28.38732889249841	22.3970409395606
23	20.826828478011134	28.21564279191897	24.222257756382472	26.73527097368742
24	16.359833868449734	33.695501235045846	27.172481411192418	22.772183485312
25	16.883728954776704	29.407767112854156	27.491236855282924	26.217267077086216
26	19.959140391259158	32.1530615250607	23.749910580155948	24.137887503524194
27	16.288718792474427	30.339837487323422	27.433166556557527	25.938277163644624
28	19.022020425596377	30.331000702734773	26.300164532513055	24.34681433915579
29	17.787816178048583	29.94849416982617	27.900884940856663	24.362804711268584
30	25.757123079577347	27.321444351401052	23.99649895010583	22.92493361891577
31	23.21233951767988	31.319247779231873	20.70416549193538	24.764247211152863
32	23.7177194362973	26.55054009585807	24.89974457484546	24.831995892999164
33	27.21771733230097	28.11465096804871	21.575219972816367	23.092411726833948
34	16.953371233320567	31.01669310688722	25.284986302983885	26.744949356808323
35	20.918352318393556	32.8629498870154	25.011466780001935	21.207231014589112
36	21.484327331333134	28.087509415383575	26.653425516425898	23.77473773685739
37	22.703803604566513	29.86980470706059	23.728660217216582	23.697731471156313
38	23.41516476395265	29.056189326005814	25.464667589619722	22.06397832042181
39	20.9972521807922	27.513539216387606	29.218828242363543	22.270380360456652
40	25.021565962388966	30.20265692656632	22.971642337455762	21.804134773588952
41	18.465513396144637	28.729228296225852	28.86472565991845	23.940532647711063
42	23.014563862600625	29.618587545183324	23.51342139259309	23.853427199622963
43	21.201129425230285	24.430974192381008	27.958744839949002	26.409151542439712
44	22.24934039715035	24.39520625476029	25.71609515113006	27.639358196959307
45	25.531364273300706	27.519851205379496	24.321355983555165	22.62742853776463
46	18.616369933050837	24.761301616289984	34.155014033655526	22.467314417003657
47	22.085228683361176	26.814381235719125	25.013570776332568	26.086819304587134
48	20.64104560201647	23.113451690140252	34.39970880690784	21.845793900935437
49	23.230854685389428	21.99707123710776	29.82562078411735	24.946453293385456
50	19.91642926574736	22.951654372314774	27.127035090450804	30.00488127148706
51	18.221239422158447	23.03644542443918	33.574942245300726	25.167372908101648
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4430.0
1	3475.0
2	2520.0
3	1731.5
4	943.0
5	1176.5
6	1410.0
7	1298.5
8	1187.0
9	1137.5
10	1088.0
11	1157.0
12	1226.0
13	1238.5
14	1251.0
15	1229.5
16	1208.0
17	1289.5
18	1371.0
19	1386.5
20	1402.0
21	1379.0
22	1356.0
23	1478.0
24	1600.0
25	2044.0
26	3053.0
27	3618.0
28	4146.5
29	4675.0
30	5638.0
31	6601.0
32	7394.5
33	8188.0
34	9011.0
35	9834.0
36	11620.0
37	13406.0
38	14050.5
39	14695.0
40	17154.0
41	19613.0
42	23418.5
43	27224.0
44	42753.5
45	58283.0
46	58938.5
47	59594.0
48	58178.0
49	56762.0
50	51325.0
51	45888.0
52	40767.5
53	35647.0
54	30720.0
55	25793.0
56	21531.0
57	17269.0
58	15077.0
59	12885.0
60	11725.5
61	10566.0
62	9185.5
63	7805.0
64	6557.5
65	5310.0
66	4121.5
67	2933.0
68	2338.0
69	1743.0
70	1472.5
71	1202.0
72	1072.5
73	943.0
74	720.5
75	424.5
76	351.0
77	309.5
78	268.0
79	166.5
80	65.0
81	55.5
82	46.0
83	46.5
84	47.0
85	30.5
86	14.0
87	9.5
88	5.0
89	8.5
90	12.0
91	12.0
92	12.0
93	8.5
94	5.0
95	4.5
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	475286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.68811114214665
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.35498826641101	27.963415297932727
2	9.253389456049579	6.604719826981306
3	3.280916939228974	3.512691851260656
4	1.6265553554625702	2.3219475321840823
5	1.0031830733536753	1.790085450888311
6	0.7395286930953168	1.5835429315195275
7	0.5773906099365168	1.4424186181892378
8	0.4492230367552897	1.2825537330668326
9	0.35632024913092475	1.1444756987863642
>10	3.948650895221174	30.29552010445572
>50	0.34095053065764913	7.844255183080811
>100	0.05821106620984254	3.7879417324200366
>500	0.004157933300703039	1.1158836688935627
>1k	0.004751923772232044	3.9087726008222647
>5k	0.0017819714145870167	5.401775769518572
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCC	9426	1.9832269412522145	No Hit
AATCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCT	8449	1.7776664997496243	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGT	7607	1.6005100087105448	No Hit
CTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTGCT	4798	1.0094974394364657	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3845	0.8089865891273886	No Hit
AATGACTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTT	1999	0.42058886649301686	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCT	1976	0.4157496749325669	No Hit
AACTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTG	1881	0.39576170979157815	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACGCTGAATCGTAT	1524	0.3206490407880729	No Hit
AAACTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCT	1280	0.2693115303206911	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGCTGAATC	1136	0.2390139831596134	No Hit
AATGCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTC	963	0.20261484663970747	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTT	940	0.19777565507925754	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	863	0.18157488333340346	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	726	0.152750133603767	No Hit
CCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTGC	666	0.1401261556199846	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	603	0.12687097873701308	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCT	503	0.10583101543070908	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.311988991891198E-4	0.0	0.0	1.2291546563542792	0.0
2	6.311988991891198E-4	0.0	0.0	1.6604739041335113	0.0
3	6.311988991891198E-4	0.0	0.0	2.5830762951149415	0.0
4	6.311988991891198E-4	0.0	0.0	6.530383811010633	0.0
5	6.311988991891198E-4	0.0	0.0	7.157585117171555	0.0
6	6.311988991891198E-4	0.0	0.0	9.452624314623195	0.0
7	0.0010519981653151996	0.0	0.0	11.343275417327671	0.0
8	0.0010519981653151996	0.0	0.0	12.490795016053491	0.0
9	0.0010519981653151996	0.0	0.0	15.637952727410443	0.0
10	0.0010519981653151996	0.0	0.0	17.661997197476886	0.0
11	0.0010519981653151996	0.0	0.0	21.847687497633004	0.0
12	0.0010519981653151996	0.0	0.0	23.016036660032064	0.0
13	0.0010519981653151996	0.0	0.0	23.612940419031908	0.0
14	0.0010519981653151996	0.0	0.0	24.43118459201407	0.0
15	0.0016831970645043194	0.0	0.0	24.873023821446456	0.0
16	0.0016831970645043194	0.0	0.0	25.594273763586557	0.0
17	0.0016831970645043194	0.0	0.0	26.458595456209526	0.0
18	0.0016831970645043194	0.0	0.0	28.030491114823494	0.0
19	0.0016831970645043194	0.0	0.0	28.60740690868235	0.0
20	0.0016831970645043194	0.0	0.0	29.1866370985049	0.0
21	0.0016831970645043194	0.0	0.0	29.787117651266815	0.0
22	0.0016831970645043194	0.0	0.0	30.412846159996295	0.0
23	0.0016831970645043194	0.0	0.0	30.979241972202	0.0
24	0.0016831970645043194	0.0	0.0	31.404459630622405	0.0
25	0.0016831970645043194	0.0	0.0	31.762349406462636	0.0
26	0.0016831970645043194	0.0	0.0	32.126340771661695	0.0
27	0.0016831970645043194	0.0	0.0	32.535357658336245	0.0
28	0.0018935966975673593	0.0	0.0	32.898507425003054	0.0
29	0.0018935966975673593	0.0	0.0	33.29490033369382	0.0
30	0.0018935966975673593	0.0	0.0	33.88317770773808	0.0
31	0.0018935966975673593	0.0	0.0	34.26442184284831	0.0
32	0.0018935966975673593	0.0	0.0	34.64250998346259	0.0
33	0.0021039963306303993	0.0	0.0	34.98987977764967	0.0
34	0.002314395963693439	0.0	0.0	35.36923031606233	0.0
35	0.002314395963693439	0.0	0.0	35.759942434660395	0.0
36	0.002314395963693439	0.0	0.0	36.113413818206304	0.0
37	0.002314395963693439	0.0	0.0	36.4771947837723	0.0
38	0.002314395963693439	0.0	0.0	36.86685490420505	0.0
39	0.002314395963693439	0.0	0.0	37.26514140959338	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTC	25	3.8873404E-5	45.000004	23
AGACTCG	20	7.0291053E-4	45.000004	44
CGGCGGA	20	7.0291053E-4	45.000004	17
CCTAGCG	30	2.1628039E-6	45.000004	1
CGTGATA	20	7.0291053E-4	45.000004	45
AACCACT	20	7.0291053E-4	45.000004	35
ATTACGG	30	2.1628039E-6	45.000004	2
TCTTACG	25	3.8873404E-5	45.000004	1
CTCCCGT	20	7.0291053E-4	45.000004	15
TCGGGTA	35	1.209919E-7	45.000004	5
GCACGCG	30	2.1628039E-6	45.000004	1
ATTCGGC	20	7.0291053E-4	45.000004	10
TTTCCGA	20	7.0291053E-4	45.000004	16
CGTTCGC	30	2.1628039E-6	45.000004	18
TCGGCAA	20	7.0291053E-4	45.000004	12
CGCCTTG	20	7.0291053E-4	45.000004	19
AGTGCGC	25	3.8873404E-5	45.000004	13
GCATTAG	30	2.1628039E-6	45.000004	1
GAGCGTT	25	3.8873404E-5	45.000004	22
CTACGCA	35	1.209919E-7	45.000004	13
>>END_MODULE