SRR3548670_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548670_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	314658
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3874	1.231177977359546	No Hit
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT	3574	1.1358363683745527	TruSeq Adapter, Index 16 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCC	2728	0.8669730310368717	No Hit
AATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	2672	0.8491759306930065	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGT	2338	0.7430289393563806	No Hit
AAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	1660	0.5275569030502959	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT	1197	0.380413019850123	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	932	0.29619459858004565	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	769	0.244392324364866	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	714	0.22691302938428387	No Hit
AACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTG	694	0.22055692211861766	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	653	0.20752690222400194	No Hit
AATGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT	637	0.20244201641146897	No Hit
CCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	579	0.18400930534103693	TruSeq Adapter, Index 13 (95% over 21bp)
AATGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTC	544	0.17288611762612108	No Hit
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTC	482	0.15318218510255577	No Hit
GGAATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCC	414	0.13157142039929065	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTAT	410	0.1303001989461574	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	389	0.12362628631720789	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	377	0.11981262195780815	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	368	0.11695237368825837	No Hit
AGCCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	343	0.1090072396061756	No Hit
AGCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCACTCGCTT	343	0.1090072396061756	No Hit
GCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	326	0.10360454843035931	TruSeq Adapter, Index 13 (95% over 21bp)
GAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT	325	0.103286743067076	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTCCC	45	3.8198777E-10	45.000004	38
CATACGG	55	1.8189894E-12	45.000004	2
CGATAGG	45	3.8198777E-10	45.000004	2
AACACTC	45	3.8198777E-10	45.000004	30
GCATATC	45	3.8198777E-10	45.000004	28
CCACAAT	20	7.0254697E-4	45.0	19
TGATCCA	20	7.0254697E-4	45.0	31
GTGTTAC	25	3.8843275E-5	45.0	10
CGGGTTC	20	7.0254697E-4	45.0	6
CTAGAGA	25	3.8843275E-5	45.0	30
AACGTAT	25	3.8843275E-5	45.0	10
ACTATGG	25	3.8843275E-5	45.0	2
ATCTCGA	20	7.0254697E-4	45.0	45
CACGGTT	20	7.0254697E-4	45.0	44
CGAAACG	20	7.0254697E-4	45.0	1
CCGATAG	20	7.0254697E-4	45.0	1
GTTTAGC	20	7.0254697E-4	45.0	14
CGCATCG	20	7.0254697E-4	45.0	21
CAGCACG	40	6.7884685E-9	45.0	1
TAGCGAT	20	7.0254697E-4	45.0	45

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content