##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548670_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	314658
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90245282179382	31.0	31.0	33.0	28.0	34.0
2	31.171436925169548	31.0	31.0	34.0	28.0	34.0
3	31.390900596838474	31.0	31.0	34.0	30.0	34.0
4	35.39378309148345	37.0	35.0	37.0	33.0	37.0
5	34.703166612639755	35.0	35.0	37.0	32.0	37.0
6	34.777771421670515	35.0	35.0	37.0	32.0	37.0
7	34.67710339479689	35.0	35.0	37.0	32.0	37.0
8	35.132969763997735	36.0	35.0	37.0	32.0	37.0
9	36.46966547807461	38.0	35.0	39.0	32.0	39.0
10	35.9462305105861	37.0	35.0	39.0	31.0	39.0
11	36.19309218262367	37.0	35.0	39.0	32.0	39.0
12	35.98257473193117	37.0	35.0	39.0	32.0	39.0
13	35.611308150436344	37.0	35.0	39.0	30.0	39.0
14	36.567864157275515	38.0	35.0	40.0	31.0	41.0
15	36.64866299283667	38.0	35.0	40.0	31.0	41.0
16	36.496056035441654	38.0	35.0	40.0	31.0	41.0
17	36.38238659115611	38.0	35.0	40.0	31.0	41.0
18	36.249025926561536	38.0	35.0	40.0	30.0	41.0
19	36.2593990936191	38.0	34.0	40.0	30.0	41.0
20	36.14658772381443	38.0	34.0	40.0	30.0	41.0
21	36.217413827075745	38.0	34.0	40.0	30.0	41.0
22	36.090409269746836	38.0	34.0	40.0	30.0	41.0
23	36.04906597003731	38.0	34.0	40.0	30.0	41.0
24	35.65061431776723	38.0	34.0	40.0	29.0	41.0
25	35.71619663253437	38.0	34.0	40.0	29.0	41.0
26	35.706938962301926	38.0	34.0	40.0	29.0	41.0
27	35.62180526158559	38.0	34.0	40.0	29.0	41.0
28	35.3786492000839	38.0	34.0	40.0	27.0	41.0
29	34.84708159334897	37.0	33.0	40.0	25.0	41.0
30	34.94444126639081	37.0	33.0	40.0	25.0	41.0
31	34.809691792358684	37.0	33.0	40.0	25.0	41.0
32	34.87892569074996	37.0	34.0	40.0	25.0	41.0
33	34.79516490920301	37.0	33.0	40.0	25.0	41.0
34	34.768087892251266	37.0	34.0	40.0	25.0	41.0
35	34.680395858360505	37.0	33.0	40.0	25.0	41.0
36	34.73837626883791	37.0	33.0	40.0	25.0	41.0
37	34.6141683987059	37.0	33.0	40.0	24.0	41.0
38	34.473491854648536	37.0	33.0	40.0	24.0	41.0
39	34.37488002847536	37.0	33.0	40.0	24.0	41.0
40	34.15762828213489	37.0	33.0	40.0	23.0	41.0
41	34.22573079343287	37.0	33.0	40.0	23.0	41.0
42	34.089926205594644	37.0	33.0	40.0	23.0	41.0
43	34.03093199600836	37.0	33.0	40.0	23.0	41.0
44	33.9591143400136	37.0	33.0	40.0	23.0	41.0
45	33.75336079171672	36.0	33.0	40.0	23.0	41.0
46	33.66947288802446	36.0	33.0	39.0	22.0	41.0
47	33.58503200300008	36.0	32.0	39.0	22.0	40.0
48	33.63753027096085	36.0	33.0	39.0	23.0	41.0
49	33.46202861519491	36.0	32.0	39.0	22.0	40.0
50	33.33960681120455	36.0	32.0	39.0	20.0	40.0
51	32.010080786123346	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	10.0
12	6.0
13	17.0
14	15.0
15	43.0
16	80.0
17	162.0
18	375.0
19	701.0
20	1306.0
21	1992.0
22	2655.0
23	3047.0
24	3585.0
25	3667.0
26	4088.0
27	4700.0
28	5569.0
29	7015.0
30	9005.0
31	11517.0
32	15367.0
33	20835.0
34	31009.0
35	33296.0
36	35548.0
37	45757.0
38	49006.0
39	24278.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.217690317741802	37.91640447724196	18.45622866731499	24.409676537701248
2	21.822105269848535	36.70715506994896	17.99827113882374	23.47246852137877
3	18.404108587736527	34.077315688779564	22.161203592471825	25.357372131012085
4	19.758595046049997	34.51525147938397	18.01066554799179	27.71548792657425
5	15.81558390379396	37.800723325006835	19.16938390252274	27.21430886867647
6	20.13392318008759	36.307673728301836	21.55578437541712	22.002618716193453
7	71.00534548621043	12.85808719307947	9.243686796458377	6.892880524251726
8	72.79490748685875	9.118153677961468	7.976596813047817	10.110342022131965
9	68.38726490348252	12.199912285719734	12.764334610910893	6.648488199886861
10	34.32583948286712	32.9303561326901	13.990427702457906	18.753376681984886
11	26.840887566818576	29.489477464421686	25.348155775476865	18.321479193282865
12	27.398318174017504	22.76471597734683	32.368158445041914	17.468807403593743
13	25.560131952786836	31.571102593927375	23.683491282598883	19.185274170686906
14	15.214613961825219	34.82161584958908	30.93040698154822	19.033363207037482
15	12.843468146368439	30.18165754565274	34.23431153824152	22.740562769737302
16	14.783987694576334	30.243311786129702	31.424912126817052	23.547788392476914
17	14.669577763794342	27.94144753986868	27.097992105714773	30.2909825906222
18	21.21446141525084	26.79830164813861	25.850288249464498	26.136948687146045
19	21.496672577846425	32.603016608508284	25.951668160351876	19.948642653293415
20	21.45408665916646	30.510903902014253	23.294497517940112	24.740511920879175
21	19.85202982285529	32.74602902198577	24.31941981452879	23.082521340630144
22	17.030236002262775	32.80100934983379	24.63214029199957	25.53661435590387
23	18.81820897609468	30.221383216063153	21.71309803024236	29.247309777599806
24	15.385911052634924	31.944206090421982	26.15951286793916	26.510369989003934
25	18.263638617165302	30.7975643396958	24.74114753160574	26.197649511533154
26	20.141550508806386	32.22101456184174	21.11721297408615	26.520221955265715
27	16.017708114842147	30.437808668459088	25.212770690718177	28.331712525980592
28	19.4919563462553	30.288440147715935	26.77827991025177	23.441323595777003
29	18.79818723820783	30.123816969535177	25.947536690629192	25.1304591016278
30	21.383533868517564	29.562890503340135	24.50056887160028	24.553006756542022
31	21.754730532832472	32.15745348918509	18.758779373160703	27.329036604821745
32	23.54016106375811	26.631453832414877	23.588467478977176	26.239917624849834
33	20.874727481900983	27.443446535603734	23.824279058533392	27.85754692396189
34	19.61590043793579	26.27169816117817	26.08387519147773	28.028526209408312
35	19.005078529705266	26.522446592808702	27.79811732102791	26.674357556458123
36	20.365285484557837	24.330225196880424	30.457512600982657	24.846976717579086
37	19.603823834131028	26.792898956962794	26.90063497511584	26.702642233790336
38	21.298997641884203	25.337350393125234	27.429463099619266	25.934188865371294
39	22.769800863159368	22.511107297446753	28.22270528637441	26.49638655301947
40	24.151936388078486	24.191344253125617	25.966922817789474	25.689796541006427
41	18.49945019672152	20.27248631847911	32.80514081955647	28.4229226652429
42	23.800761461650428	24.327682753974155	27.618557290772838	24.25299849360258
43	19.02446465686555	23.95775731111238	29.090949538864418	27.92682849315765
44	21.222088743969643	21.58756491174545	27.05794862994108	30.132397714343828
45	22.980823624379486	25.19084212065163	25.73619612404579	26.092138130923097
46	19.915590895511954	24.067400161445125	31.481163676118197	24.535845266924724
47	20.290601224186258	23.822372226353693	25.932917643918156	29.954108905541887
48	20.421854839222267	20.17714470949412	34.91123696203497	24.489763489248645
49	22.438329869254872	19.250424270159982	31.651825156201337	26.659420704383805
50	19.227542284003583	20.618894164457917	29.45515448518709	30.698409066351406
51	19.885717191363323	21.060961424785006	32.81181473218542	26.241506651666253
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4416.0
1	3514.0
2	2612.0
3	1806.0
4	1000.0
5	1093.5
6	1187.0
7	1161.5
8	1136.0
9	1117.0
10	1098.0
11	1148.5
12	1199.0
13	1223.0
14	1247.0
15	1240.0
16	1233.0
17	1248.5
18	1264.0
19	1162.5
20	1061.0
21	1041.5
22	1022.0
23	1126.0
24	1230.0
25	1479.0
26	1908.0
27	2088.0
28	2351.5
29	2615.0
30	2869.0
31	3123.0
32	3455.5
33	3788.0
34	4718.5
35	5649.0
36	5710.0
37	5771.0
38	6160.0
39	6549.0
40	8075.0
41	9601.0
42	13102.5
43	16604.0
44	23174.0
45	29744.0
46	31670.5
47	33597.0
48	34836.0
49	36075.0
50	33482.5
51	30890.0
52	27695.5
53	24501.0
54	20917.5
55	17334.0
56	15939.5
57	14545.0
58	12945.5
59	11346.0
60	10508.0
61	9670.0
62	8684.0
63	7698.0
64	7019.0
65	6340.0
66	5288.5
67	4237.0
68	3583.0
69	2929.0
70	2746.0
71	2563.0
72	2330.5
73	2098.0
74	1716.5
75	1160.5
76	986.0
77	739.0
78	492.0
79	493.0
80	494.0
81	405.5
82	317.0
83	222.5
84	128.0
85	105.5
86	83.0
87	46.5
88	10.0
89	14.5
90	19.0
91	11.5
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	314658.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.01807723133452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.59621573618455	32.25305585883932
2	8.565845354776632	6.855773219182387
3	3.0375715980470845	3.6467332441906932
4	1.4568944453001873	2.3320845771970067
5	0.8889156599928801	1.7786347766868889
6	0.5754902300865574	1.3818007484089398
7	0.44089623018027313	1.2350673573250888
8	0.3636845370444285	1.1643164713028866
9	0.28489657888661923	1.0260911967144946
>10	3.5054897976422015	31.131988386142318
>50	0.22441499323285155	5.651125891620188
>100	0.04774787090060671	4.038747734997307
>500	0.0063663827867475615	1.758552672500799
>1k	0.0055705849384041165	5.7460278648916905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3874	1.231177977359546	No Hit
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT	3574	1.1358363683745527	TruSeq Adapter, Index 16 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCC	2728	0.8669730310368717	No Hit
AATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	2672	0.8491759306930065	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGT	2338	0.7430289393563806	No Hit
AAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	1660	0.5275569030502959	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT	1197	0.380413019850123	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	932	0.29619459858004565	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	769	0.244392324364866	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	714	0.22691302938428387	No Hit
AACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTG	694	0.22055692211861766	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	653	0.20752690222400194	No Hit
AATGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT	637	0.20244201641146897	No Hit
CCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	579	0.18400930534103693	TruSeq Adapter, Index 13 (95% over 21bp)
AATGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTC	544	0.17288611762612108	No Hit
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTC	482	0.15318218510255577	No Hit
GGAATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCC	414	0.13157142039929065	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTAT	410	0.1303001989461574	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	389	0.12362628631720789	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	377	0.11981262195780815	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	368	0.11695237368825837	No Hit
AGCCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	343	0.1090072396061756	No Hit
AGCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCACTCGCTT	343	0.1090072396061756	No Hit
GCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	326	0.10360454843035931	TruSeq Adapter, Index 13 (95% over 21bp)
GAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT	325	0.103286743067076	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.534160898499323E-4	0.0	0.0	1.3411386330555715	0.0
2	9.534160898499323E-4	0.0	0.0	1.8556655162112516	0.0
3	9.534160898499323E-4	0.0	0.0	2.5910671268488326	0.0
4	9.534160898499323E-4	0.0	0.0	5.930248078866579	0.0
5	9.534160898499323E-4	0.0	0.0	6.651984058882977	0.0
6	9.534160898499323E-4	0.0	0.0	9.314239587107272	0.0
7	9.534160898499323E-4	0.0	0.0	11.384741528898042	0.0
8	9.534160898499323E-4	0.0	0.0	12.782449516618042	0.0
9	9.534160898499323E-4	0.0	0.0	15.07636862879698	0.0
10	9.534160898499323E-4	0.0	0.0	17.506308436461175	0.0
11	9.534160898499323E-4	0.0	0.0	20.685633290747415	0.0
12	9.534160898499323E-4	0.0	0.0	22.023911675533437	0.0
13	9.534160898499323E-4	0.0	0.0	22.7786994133313	0.0
14	9.534160898499323E-4	0.0	0.0	23.455307031761468	0.0
15	9.534160898499323E-4	0.0	0.0	23.922798721151217	0.0
16	9.534160898499323E-4	0.0	0.0	24.777059537656758	0.0
17	9.534160898499323E-4	0.0	0.0	25.66310089049063	0.0
18	9.534160898499323E-4	0.0	0.0	27.416750885087936	0.0
19	9.534160898499323E-4	0.0	0.0	27.896636983645735	0.0
20	9.534160898499323E-4	0.0	0.0	28.714667988736977	0.0
21	9.534160898499323E-4	0.0	0.0	29.23968244888101	0.0
22	9.534160898499323E-4	0.0	0.0	29.787578895181436	0.0
23	9.534160898499323E-4	0.0	0.0	30.330072650306047	0.0
24	9.534160898499323E-4	0.0	0.0	30.679340744554406	0.0
25	9.534160898499323E-4	0.0	0.0	30.99301463811503	0.0
26	9.534160898499323E-4	0.0	0.0	31.266645055901964	0.0
27	9.534160898499323E-4	0.0	0.0	31.680427638896834	0.0
28	9.534160898499323E-4	0.0	0.0	31.98234273401598	0.0
29	9.534160898499323E-4	0.0	0.0	32.28870710422109	0.0
30	9.534160898499323E-4	0.0	0.0	32.84486648996688	0.0
31	9.534160898499323E-4	0.0	0.0	33.13692961882425	0.0
32	9.534160898499323E-4	0.0	0.0	33.46363353227949	0.0
33	9.534160898499323E-4	0.0	0.0	33.790655251098016	0.0
34	9.534160898499323E-4	0.0	0.0	34.14500823115891	0.0
35	9.534160898499323E-4	0.0	0.0	34.50031462730965	0.0
36	9.534160898499323E-4	0.0	0.0	34.75741916620585	0.0
37	9.534160898499323E-4	0.0	0.0	35.04344399316083	0.0
38	9.534160898499323E-4	0.0	0.0	35.33582492738147	0.0
39	9.534160898499323E-4	0.0	0.0	35.673652028551636	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTCCC	45	3.8198777E-10	45.000004	38
CATACGG	55	1.8189894E-12	45.000004	2
CGATAGG	45	3.8198777E-10	45.000004	2
AACACTC	45	3.8198777E-10	45.000004	30
GCATATC	45	3.8198777E-10	45.000004	28
CCACAAT	20	7.0254697E-4	45.0	19
TGATCCA	20	7.0254697E-4	45.0	31
GTGTTAC	25	3.8843275E-5	45.0	10
CGGGTTC	20	7.0254697E-4	45.0	6
CTAGAGA	25	3.8843275E-5	45.0	30
AACGTAT	25	3.8843275E-5	45.0	10
ACTATGG	25	3.8843275E-5	45.0	2
ATCTCGA	20	7.0254697E-4	45.0	45
CACGGTT	20	7.0254697E-4	45.0	44
CGAAACG	20	7.0254697E-4	45.0	1
CCGATAG	20	7.0254697E-4	45.0	1
GTTTAGC	20	7.0254697E-4	45.0	14
CGCATCG	20	7.0254697E-4	45.0	21
CAGCACG	40	6.7884685E-9	45.0	1
TAGCGAT	20	7.0254697E-4	45.0	45
>>END_MODULE