SRR3548669_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548669_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	319044
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3938	1.2343125086195008	No Hit
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT	3541	1.1098782613056506	TruSeq Adapter, Index 16 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	2921	0.9155476987500157	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCC	2708	0.8487857474204185	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGT	2439	0.7644713581825704	No Hit
AAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	1594	0.4996176076027131	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT	1263	0.3958701621093016	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	898	0.2814658793144519	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	727	0.2278682564160429	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	724	0.22692794724238663	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	720	0.22567420167751157	No Hit
AACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTG	706	0.22128609220044884	No Hit
AATGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT	669	0.20968894572535451	No Hit
AATGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTC	605	0.18962901668735346	No Hit
CCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	580	0.18179310690688433	TruSeq Adapter, Index 13 (95% over 21bp)
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTC	546	0.17113626960544626	No Hit
GGAATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCC	458	0.14355386717819485	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTAT	435	0.13634483018016325	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	427	0.13383733905041312	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	391	0.12255362896653754	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	379	0.11879239227191234	No Hit
AGCCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	348	0.10907586414413059	No Hit
GCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	327	0.10249369992853652	TruSeq Adapter, Index 13 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTG	322	0.10092651797244268	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTCG	20	7.025616E-4	45.000004	1
CGACGTC	20	7.025616E-4	45.000004	14
ACTTGCG	45	3.8380676E-10	45.000004	1
TCACAAG	20	7.025616E-4	45.000004	1
TTTAACG	25	3.8844482E-5	45.000004	1
ATTACGG	20	7.025616E-4	45.000004	2
AACCAAG	25	3.8844482E-5	45.000004	1
GACAAAC	40	6.7884685E-9	45.000004	12
GTCTCGA	20	7.025616E-4	45.000004	28
TCGGGCA	45	3.8380676E-10	45.000004	5
GAATCAA	20	7.025616E-4	45.000004	9
ATAGAAC	50	2.1827873E-11	45.000004	37
CGACAAT	40	6.7884685E-9	45.000004	20
CATTCCG	25	3.8844482E-5	45.000004	1
ACGGGTA	20	7.025616E-4	45.000004	5
CGTTCAT	20	7.025616E-4	45.000004	17
GCTTGAC	20	7.025616E-4	45.000004	10
TAGCCGT	20	7.025616E-4	45.000004	44
ATCGTCG	25	3.8844482E-5	45.000004	1
TTATAGG	25	3.8844482E-5	45.000004	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content