SRR3548667_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548667_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	307881
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCC	4074	1.3232385239751723	No Hit
CTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGCT	4067	1.3209649182638747	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3933	1.2774416089333216	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCT	3622	1.1764285551885305	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGT	3529	1.1462220793098632	No Hit
AAACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCT	1261	0.40957382884945803	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	990	0.32155280774065303	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTT	879	0.28549991717579193	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTT	870	0.2825767098326951	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	777	0.2523702339540277	No Hit
AACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTG	767	0.2491222257950312	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCT	736	0.23905340050214205	No Hit
CCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	564	0.18318766016740234	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAAGAGTC	499	0.16207560713392513	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCT	487	0.15817799734312932	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTAT	466	0.1513571802092367	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	416	0.1351171394142542	No Hit
CGATGGGGGATTTTTGGGCTGGAGGGATGTCTCAGCGATTAAGAGTATGTA	378	0.12277470841006752	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	371	0.12050110269876999	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	367	0.11920189943517139	No Hit
AATGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTC	320	0.10393626108788785	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.025231E-4	45.000004	1
TCCGGAA	20	7.025231E-4	45.000004	23
GTCATCA	20	7.025231E-4	45.000004	39
CTAGACG	20	7.025231E-4	45.000004	1
AAGTAGT	20	7.025231E-4	45.000004	41
TCGTTGG	40	6.7866495E-9	45.000004	19
AGATCGT	40	6.7866495E-9	45.000004	16
CGAACAG	20	7.025231E-4	45.000004	1
CTCGTCA	20	7.025231E-4	45.000004	36
CTGTAGG	20	7.025231E-4	45.000004	2
CAAGTAG	20	7.025231E-4	45.000004	1
CGAGTAC	20	7.025231E-4	45.000004	44
ACTTGCG	20	7.025231E-4	45.000004	1
CCCTAGC	40	6.7866495E-9	45.000004	21
GTTTAGG	20	7.025231E-4	45.000004	2
CGTTGGC	40	6.7866495E-9	45.000004	20
GAGTACG	20	7.025231E-4	45.000004	1
TAGCGCG	20	7.025231E-4	45.000004	1
CGACCTT	20	7.025231E-4	45.000004	37
TAAACCG	20	7.025231E-4	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content