FastQCFastQC Report
Sat 18 Jun 2016
SRR3548665_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548665_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477829
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT66651.394850459055436No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43990.920622230965471No Hit
AATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT38630.8084482105523106No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC37200.7785211864495457No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGT33980.7111330622461174No Hit
AAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT25050.5242461215204602No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT16680.3490788545693125No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10070.21074484805233673No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC9740.20383861172092946No Hit
AACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG9580.20049013349964107No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT9250.19358389716823382No Hit
AATGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT8800.1841663021708603No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT8260.17286518817401206No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7730.1617733540659943No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT5770.1207544958552118No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG5770.1207544958552118No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTAT5530.11573177852327926No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC4960.10380282485993944No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.10150074608280368No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG207.0291426E-445.0000041
ACACGTC207.0291426E-445.00000426
ATCTACT207.0291426E-445.00000421
CGTGCAC302.1628293E-645.00000433
CAGCCGA207.0291426E-445.00000443
TTGTACG207.0291426E-445.0000041
TTATCCG207.0291426E-445.0000041
ACCTCGG207.0291426E-445.0000042
CCGTAAG207.0291426E-445.00000443
GTTCGGG406.8012014E-945.0000043
CATAGCG302.1628293E-645.0000041
CGTAGCG302.1628293E-645.0000041
TGCGAAC207.0291426E-445.00000440
TATTCGA207.0291426E-445.00000417
TATTACG406.8012014E-945.0000041
AATTCGT207.0291426E-445.00000431
CGTAAGA207.0291426E-445.00000444
TCGAGTG207.0291426E-445.00000420
GAACCCG207.0291426E-445.0000041
ATACGTA207.0291426E-445.00000414
Produced by FastQC (version 0.11.5)