##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548665_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	477829
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.985756410766193	31.0	31.0	33.0	28.0	34.0
2	31.266105657044676	31.0	31.0	34.0	28.0	34.0
3	31.506511743740962	31.0	31.0	34.0	30.0	34.0
4	35.4531767640725	37.0	35.0	37.0	33.0	37.0
5	34.720312078170224	35.0	35.0	37.0	32.0	37.0
6	34.82010719315906	35.0	35.0	37.0	32.0	37.0
7	34.83943628369145	35.0	35.0	37.0	32.0	37.0
8	35.186148182718085	36.0	35.0	37.0	32.0	37.0
9	36.71704521910558	39.0	37.0	39.0	32.0	39.0
10	36.10049410981753	37.0	35.0	39.0	31.0	39.0
11	36.31480299437665	38.0	35.0	39.0	32.0	39.0
12	36.004997603745274	37.0	35.0	39.0	32.0	39.0
13	35.75269395536897	37.0	35.0	39.0	30.0	39.0
14	36.7285304994046	38.0	35.0	40.0	31.0	41.0
15	36.718533199115164	38.0	35.0	40.0	31.0	41.0
16	36.59933783843174	38.0	35.0	40.0	31.0	41.0
17	36.52373338579282	38.0	35.0	40.0	31.0	41.0
18	36.38289429900655	38.0	35.0	40.0	31.0	41.0
19	36.45994487567728	38.0	35.0	40.0	31.0	41.0
20	36.33999401459518	38.0	35.0	40.0	30.0	41.0
21	36.43156861555075	38.0	35.0	40.0	31.0	41.0
22	36.44479510452484	38.0	35.0	40.0	30.0	41.0
23	36.33553635296309	38.0	35.0	40.0	30.0	41.0
24	35.89832973720724	38.0	34.0	40.0	29.0	41.0
25	36.15893761157234	38.0	34.0	40.0	30.0	41.0
26	35.99324025959078	38.0	34.0	40.0	30.0	41.0
27	35.90012326585452	38.0	34.0	40.0	29.0	41.0
28	35.89328399908754	38.0	34.0	40.0	29.0	41.0
29	35.52087671531029	38.0	34.0	40.0	27.0	41.0
30	35.59986522375159	38.0	34.0	40.0	28.0	41.0
31	35.52368525141839	38.0	34.0	40.0	28.0	41.0
32	35.5040715402372	38.0	34.0	40.0	28.0	41.0
33	35.50882219371365	38.0	34.0	40.0	28.0	41.0
34	35.44776060054957	38.0	34.0	40.0	28.0	41.0
35	35.40916729625033	38.0	34.0	40.0	27.0	41.0
36	35.40419689889061	38.0	34.0	40.0	27.0	41.0
37	35.21577802937871	38.0	34.0	40.0	27.0	41.0
38	35.18881022290401	38.0	34.0	40.0	27.0	41.0
39	35.167528551008836	38.0	34.0	40.0	27.0	41.0
40	34.91612271335561	37.0	34.0	40.0	26.0	41.0
41	35.04152112994397	38.0	34.0	40.0	26.0	41.0
42	34.96667636330152	37.0	34.0	40.0	26.0	41.0
43	34.97471061823372	37.0	34.0	40.0	26.0	41.0
44	34.82061574328892	37.0	34.0	40.0	26.0	41.0
45	34.63131580544504	37.0	33.0	40.0	25.0	41.0
46	34.6013134405823	37.0	33.0	40.0	25.0	41.0
47	34.49461418206095	37.0	33.0	40.0	25.0	41.0
48	34.5278352716139	37.0	33.0	40.0	25.0	41.0
49	34.42482561753263	37.0	33.0	40.0	24.0	41.0
50	34.3330103447049	37.0	33.0	40.0	24.0	41.0
51	33.03924835035128	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	13.0
12	11.0
13	14.0
14	27.0
15	70.0
16	99.0
17	225.0
18	433.0
19	795.0
20	1456.0
21	2038.0
22	2747.0
23	3257.0
24	3728.0
25	4309.0
26	4837.0
27	5722.0
28	7307.0
29	9354.0
30	12146.0
31	15712.0
32	21763.0
33	30165.0
34	45232.0
35	49935.0
36	56508.0
37	74222.0
38	82699.0
39	42999.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.26295808751666	35.50433314009824	19.69302825906339	25.53968051332171
2	21.10922526677954	35.636807309727956	19.939350688216916	23.31461673527559
3	20.67873653545515	32.52607104215106	21.62991363018988	25.165278792203903
4	21.158615320543543	32.0566562515042	19.512838274780307	27.271890153171952
5	16.94016060138669	37.079373583436755	19.10599817089377	26.87446764428279
6	19.757067905045528	35.95784265919398	22.119000730386812	22.166088705373678
7	72.4246540080238	10.376096888217333	10.047946022531073	7.151303081227803
8	74.31947412149535	7.3982533500478205	8.160659985057416	10.121612543399417
9	68.99957934742345	10.411883749207353	13.927995161448969	6.660541741920227
10	34.11471467826356	32.69684343143677	14.831665721419169	18.356776168880497
11	27.11576735610438	25.389208273252567	27.94053939798547	19.554484972657583
12	27.28005206883634	22.960933723152007	31.679743171720425	18.079271036291225
13	23.73945490960155	29.393778946024625	26.370103112201228	20.496663032172595
14	15.361353120049223	31.509807901990044	33.23720410439718	19.891634873563554
15	14.279585374684247	29.45112163556419	33.68610946593865	22.58318352381291
16	16.27527839457212	28.177653512030453	32.6524760950047	22.89459199839273
17	15.87220532868453	27.727283191267173	29.55576157997945	26.844749900068855
18	18.543872389494986	26.475998735949474	28.490526945832084	26.489601928723456
19	19.645312444410028	32.365344087529216	28.398025234968994	19.591318233091755
20	21.727647338273734	30.61911269512734	26.139685954598825	21.513554012000107
21	18.999265427590206	31.16072904742073	26.384124864752874	23.455880660236193
22	17.071379091683426	30.131071994374558	26.82193839218633	25.975610521755694
23	19.628779333192416	29.6089186717424	24.246330800349078	26.515971194716105
24	15.943569770775737	31.574475387638675	27.68814785205586	24.793806989529728
25	18.628421464582516	27.52407241921273	26.674396070560807	27.173110045643945
26	20.394325166534472	30.484964286386973	23.463414736234093	25.657295810844467
27	15.613744665978835	28.642045585345382	26.979316868586878	28.764892880088905
28	20.110960197057942	26.571639645145023	27.213710344077064	26.10368981371997
29	18.902159559172844	28.46018136195166	26.514506235494288	26.12315284338121
30	20.427391388969692	26.279694200226437	28.421046022740352	24.87186838806351
31	24.7086300747757	27.059261786120135	21.272672859956177	26.959435279147982
32	22.232011870355294	23.60614362041651	28.47273815528149	25.689106353946705
33	22.51412116049884	22.84541122451756	28.354285738203416	26.286181876780184
34	18.274319892681273	22.73093512532726	27.748839019816714	31.245905962174753
35	18.295457161453154	21.746691808157312	34.00840049473766	25.949450535651874
36	21.513554012000107	22.728842326438954	31.60607665085208	24.15152701070885
37	19.691144740063915	26.20226064135915	29.681748073055424	24.424846545521515
38	22.31718878510932	24.55941351403954	32.29439820521567	20.82899949563547
39	19.7692061385977	22.227198432912193	32.90968107837741	25.093914350112694
40	21.53699335954913	22.30546911133481	29.824058397460178	26.333479131655885
41	18.238742311580083	19.887239995898113	32.77971826741366	29.094299425108144
42	22.743701198545924	22.951516128154633	28.089965238610464	26.21481743468898
43	18.757965715768613	22.296679356003928	31.004606250353163	27.9407486778743
44	21.011700838584517	21.167195795985595	29.64072921484464	28.18037415058525
45	25.2002285336386	23.477436488785738	27.765790690811986	23.556544286763675
46	18.505364889950172	22.539653306936163	34.25011876633691	24.704863036776757
47	20.168512166486337	23.05573751279223	28.64267342501188	28.133076895709554
48	20.595233859811774	20.195718552034307	35.47168547744067	23.73736211071325
49	21.854470950905032	19.717095446278897	32.6127129161269	25.81572068668917
50	19.843291219243707	20.339703115549703	29.234726230513424	30.582279434693167
51	18.92141330894525	20.1415150608272	34.720789236316755	26.216282393910795
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5025.0
1	3924.0
2	2823.0
3	1946.0
4	1069.0
5	1290.5
6	1512.0
7	1381.5
8	1251.0
9	1273.5
10	1296.0
11	1260.0
12	1224.0
13	1304.0
14	1384.0
15	1357.0
16	1330.0
17	1397.0
18	1464.0
19	1427.0
20	1390.0
21	1426.5
22	1463.0
23	1618.0
24	1773.0
25	2133.0
26	2827.5
27	3162.0
28	3668.0
29	4174.0
30	4871.0
31	5568.0
32	6902.0
33	8236.0
34	8810.0
35	9384.0
36	10331.5
37	11279.0
38	12383.5
39	13488.0
40	14664.5
41	15841.0
42	21110.5
43	26380.0
44	37649.5
45	48919.0
46	50818.0
47	52717.0
48	55509.5
49	58302.0
50	53388.5
51	48475.0
52	42036.0
53	35597.0
54	30781.0
55	25965.0
56	23340.5
57	20716.0
58	18422.5
59	16129.0
60	14686.0
61	13243.0
62	11710.0
63	10177.0
64	9025.5
65	7874.0
66	6632.5
67	5391.0
68	4297.0
69	3203.0
70	2944.0
71	2685.0
72	2320.5
73	1956.0
74	1662.0
75	1065.5
76	763.0
77	659.0
78	555.0
79	435.5
80	316.0
81	281.0
82	246.0
83	182.5
84	119.0
85	78.0
86	37.0
87	45.5
88	54.0
89	31.5
90	9.0
91	5.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	477829.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.111540146478596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.42783914431716	27.18610682570779
2	10.000752266482648	7.022836293991847
3	3.678898721855037	3.8751540050172584
4	1.7443873334201054	2.4499250355355513
5	1.0157364173885794	1.7832034998689728
6	0.6820532007339143	1.4368763003761835
7	0.5006762635202018	1.2305660308884925
8	0.3827271337867404	1.0750511298479857
9	0.3012868463026265	0.9520780679604539
>10	3.691428833192906	30.04500914599346
>50	0.50183896970948	11.377614689836529
>100	0.06220649922501302	4.368752021743968
>500	0.005383254740626127	1.479142413669011
>1k	0.004186975909375877	4.317928159170078
>5k	5.981394156251253E-4	1.3997563803924404
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT	6665	1.394850459055436	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4399	0.920622230965471	No Hit
AATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	3863	0.8084482105523106	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC	3720	0.7785211864495457	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGT	3398	0.7111330622461174	No Hit
AAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	2505	0.5242461215204602	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT	1668	0.3490788545693125	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1007	0.21074484805233673	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	974	0.20383861172092946	No Hit
AACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	958	0.20049013349964107	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	925	0.19358389716823382	No Hit
AATGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT	880	0.1841663021708603	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	826	0.17286518817401206	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	773	0.1617733540659943	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	577	0.1207544958552118	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	577	0.1207544958552118	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTAT	553	0.11573177852327926	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	496	0.10380282485993944	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	485	0.10150074608280368	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0927988883052306E-4	0.0	0.0	1.6252676166578421	0.0
2	2.0927988883052306E-4	0.0	0.0	2.1723252460608293	0.0
3	2.0927988883052306E-4	0.0	0.0	2.930964843071475	0.0
4	2.0927988883052306E-4	0.0	0.0	6.426985385985363	0.0
5	2.0927988883052306E-4	0.0	0.0	7.016945392598608	0.0
6	2.0927988883052306E-4	0.0	0.0	9.674381420968588	0.0
7	2.0927988883052306E-4	0.0	0.0	11.861146979358725	0.0
8	2.0927988883052306E-4	0.0	0.0	13.31606076650852	0.0
9	2.0927988883052306E-4	0.0	0.0	15.662716159965175	0.0
10	2.0927988883052306E-4	0.0	0.0	18.134520926942482	0.0
11	2.0927988883052306E-4	0.0	0.0	21.152546203767457	0.0
12	2.0927988883052306E-4	0.0	0.0	22.544048184601603	0.0
13	4.185597776610461E-4	0.0	0.0	23.128776193994085	0.0
14	4.185597776610461E-4	0.0	0.0	23.77356753148093	0.0
15	4.185597776610461E-4	0.0	0.0	24.1977778661404	0.0
16	4.185597776610461E-4	0.0	0.0	24.981112490033045	0.0
17	4.185597776610461E-4	0.0	0.0	25.90278112044267	0.0
18	4.185597776610461E-4	0.0	0.0	27.376530097587214	0.0
19	4.185597776610461E-4	0.0	0.0	27.9187742895471	0.0
20	4.185597776610461E-4	0.0	0.0	28.47943511172407	0.0
21	4.185597776610461E-4	0.0	0.0	29.07253431666977	0.0
22	4.185597776610461E-4	0.0	0.0	29.65286744839681	0.0
23	4.185597776610461E-4	0.0	0.0	30.25643064778404	0.0
24	4.185597776610461E-4	0.0	0.0	30.67247906677912	0.0
25	4.185597776610461E-4	0.0	0.0	31.025952799013872	0.0
26	4.185597776610461E-4	0.0	0.0	31.363521259697507	0.0
27	4.185597776610461E-4	0.0	0.0	31.725156907596652	0.0
28	4.185597776610461E-4	0.0	0.0	32.10018646838095	0.0
29	4.185597776610461E-4	0.0	0.0	32.48944706160572	0.0
30	4.185597776610461E-4	0.0	0.0	33.03106341389911	0.0
31	4.185597776610461E-4	0.0	0.0	33.39207122213177	0.0
32	4.185597776610461E-4	0.0	0.0	33.742615035922896	0.0
33	4.185597776610461E-4	0.0	0.0	34.09880940671244	0.0
34	4.185597776610461E-4	0.0	0.0	34.44977178028123	0.0
35	4.185597776610461E-4	0.0	0.0	34.83547461539588	0.0
36	4.185597776610461E-4	0.0	0.0	35.16362548108214	0.0
37	4.185597776610461E-4	0.0	0.0	35.4746153958843	0.0
38	4.185597776610461E-4	0.0	0.0	35.81511377501156	0.0
39	4.185597776610461E-4	0.0	0.0	36.176540143021874	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCCG	20	7.0291426E-4	45.000004	1
ACACGTC	20	7.0291426E-4	45.000004	26
ATCTACT	20	7.0291426E-4	45.000004	21
CGTGCAC	30	2.1628293E-6	45.000004	33
CAGCCGA	20	7.0291426E-4	45.000004	43
TTGTACG	20	7.0291426E-4	45.000004	1
TTATCCG	20	7.0291426E-4	45.000004	1
ACCTCGG	20	7.0291426E-4	45.000004	2
CCGTAAG	20	7.0291426E-4	45.000004	43
GTTCGGG	40	6.8012014E-9	45.000004	3
CATAGCG	30	2.1628293E-6	45.000004	1
CGTAGCG	30	2.1628293E-6	45.000004	1
TGCGAAC	20	7.0291426E-4	45.000004	40
TATTCGA	20	7.0291426E-4	45.000004	17
TATTACG	40	6.8012014E-9	45.000004	1
AATTCGT	20	7.0291426E-4	45.000004	31
CGTAAGA	20	7.0291426E-4	45.000004	44
TCGAGTG	20	7.0291426E-4	45.000004	20
GAACCCG	20	7.0291426E-4	45.000004	1
ATACGTA	20	7.0291426E-4	45.000004	14
>>END_MODULE