FastQCFastQC Report
Sat 18 Jun 2016
SRR3548664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217007
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21791.004115074628929No Hit
CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT19260.8875289737197417No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCC12320.567723621818651No Hit
AATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCT11990.5525167390913658No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGT11400.525328676033492No Hit
AAACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCT6230.2870875133060224No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5180.2387019773555692No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTT4340.1999935485952066No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4060.18709073900841908No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG3370.15529452966954982No Hit
AACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTG3020.13916601768606543No Hit
CCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC2910.13409705677697034No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCT2580.11889017404968502No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.11796854479348594No Hit
AATGATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCT2540.11704691553728681No Hit
AATGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTT2520.1161252862810877No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTAT2220.10230084743810108No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG207.02063E-445.01
TCCGCGG207.02063E-445.02
ACGTTAG207.02063E-445.01
ATGGCAG253.8803177E-545.01
GAACTAT207.02063E-445.015
CAACCGG351.2058445E-745.02
TGAACGG207.02063E-445.02
ATTCGGG253.8803177E-545.03
TGAGTTA207.02063E-445.010
TAACGTG253.8803177E-545.01
ACGGGCG351.2058445E-745.05
ATCCGCG207.02063E-445.01
GTTACGG406.7702786E-945.02
AACGGGC253.8803177E-545.04
CTAACGG253.8803177E-545.02
TCTACGA253.8803177E-545.022
TACGACG207.02063E-445.01
TCTAAGG207.02063E-445.02
ACTACGG207.02063E-445.02
TAGGGTC406.7702786E-945.05