##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548664_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 217007 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.069334168943858 31.0 31.0 33.0 28.0 34.0 2 31.348205357430867 31.0 31.0 34.0 30.0 34.0 3 31.552576644993017 31.0 31.0 34.0 30.0 34.0 4 35.5039883506062 37.0 35.0 37.0 33.0 37.0 5 34.84041067799656 35.0 35.0 37.0 32.0 37.0 6 34.94649020538508 35.0 35.0 37.0 32.0 37.0 7 34.90192021455529 36.0 35.0 37.0 32.0 37.0 8 35.32330754307465 37.0 35.0 37.0 33.0 37.0 9 36.7745925246651 39.0 37.0 39.0 32.0 39.0 10 36.20279069338777 38.0 35.0 39.0 32.0 39.0 11 36.38836074412346 38.0 35.0 39.0 32.0 39.0 12 36.08753173860751 37.0 35.0 39.0 32.0 39.0 13 35.74388383784855 37.0 35.0 39.0 30.0 39.0 14 36.76493385006014 39.0 35.0 40.0 31.0 41.0 15 36.80818130290728 38.0 35.0 40.0 32.0 41.0 16 36.69426792684107 38.0 35.0 40.0 31.0 41.0 17 36.63025616685176 38.0 35.0 40.0 31.0 41.0 18 36.57050233402609 38.0 35.0 40.0 31.0 41.0 19 36.65353652186335 38.0 35.0 40.0 31.0 41.0 20 36.526167358656636 38.0 35.0 40.0 31.0 41.0 21 36.633523342564985 38.0 35.0 40.0 31.0 41.0 22 36.574147377734356 38.0 35.0 40.0 31.0 41.0 23 36.50221421428802 38.0 35.0 40.0 31.0 41.0 24 36.03815545120664 38.0 34.0 40.0 30.0 41.0 25 36.26092706686881 38.0 35.0 40.0 30.0 41.0 26 36.204721506679505 38.0 35.0 40.0 30.0 41.0 27 36.10105664794223 38.0 35.0 40.0 30.0 41.0 28 36.09809821802983 38.0 35.0 40.0 30.0 41.0 29 35.52105692443101 38.0 34.0 40.0 27.0 41.0 30 35.62002147396167 38.0 34.0 40.0 29.0 41.0 31 35.491974913251646 38.0 34.0 40.0 27.0 41.0 32 35.3278281345763 38.0 34.0 40.0 27.0 41.0 33 35.265521388710965 38.0 34.0 40.0 27.0 41.0 34 35.150276258369544 38.0 34.0 40.0 26.0 41.0 35 35.051486818397564 38.0 34.0 40.0 26.0 41.0 36 34.98739211177519 37.0 33.0 40.0 26.0 41.0 37 34.73432193431548 37.0 33.0 40.0 25.0 41.0 38 34.509370665462406 37.0 33.0 40.0 25.0 41.0 39 34.30328053933744 36.0 33.0 40.0 24.0 41.0 40 34.135843544217465 36.0 33.0 40.0 24.0 41.0 41 34.18652854516214 36.0 33.0 40.0 24.0 41.0 42 34.12371029505961 36.0 33.0 40.0 24.0 41.0 43 34.02288405443142 35.0 33.0 39.0 24.0 41.0 44 33.89909542088504 35.0 33.0 39.0 24.0 41.0 45 33.666886321639396 35.0 32.0 39.0 24.0 41.0 46 33.5229508725525 35.0 32.0 39.0 23.0 41.0 47 33.3869690839466 35.0 32.0 39.0 23.0 40.0 48 33.39326381176644 35.0 32.0 39.0 23.0 40.0 49 33.24553585829029 35.0 32.0 39.0 24.0 40.0 50 33.09263295654057 35.0 32.0 38.0 23.0 40.0 51 31.899957144239586 34.0 30.0 37.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 6.0 11 4.0 12 7.0 13 10.0 14 6.0 15 25.0 16 55.0 17 112.0 18 213.0 19 366.0 20 636.0 21 892.0 22 1262.0 23 1520.0 24 1819.0 25 2115.0 26 2420.0 27 2976.0 28 3713.0 29 4594.0 30 6082.0 31 8049.0 32 10830.0 33 15160.0 34 23475.0 35 24616.0 36 24718.0 37 29132.0 38 32145.0 39 20045.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.547756523983097 34.648651886805496 20.07446764390089 25.729123945310523 2 23.648545899441032 33.74315114258987 18.96713009257766 23.64117286539144 3 19.98921693770247 33.13856235052325 21.180883565967918 25.691337145806358 4 20.82743874621556 32.837650398374244 18.95560972687517 27.379301128535023 5 17.552429184312025 36.98221716350164 17.223868354477045 28.24148529770929 6 18.980032902164446 37.19234863391503 21.49884565935661 22.328772804563908 7 76.35237572981517 10.112116199016622 7.58040063223767 5.955107438930542 8 77.82467846659324 7.2827143824853575 6.447257461740866 8.445349689180533 9 72.93543526245698 10.242987553396896 11.195030575050573 5.626546609095559 10 37.66975258862617 30.834028395397382 13.935034353730524 17.561184662245918 11 29.42070993101605 28.295400609196935 23.52781246687895 18.756076992908064 12 29.840512057214745 23.698774693903886 28.42673277820531 18.033980470676063 13 24.15774606349104 31.557046546885587 24.328247475887874 19.9569599137355 14 17.368103333072206 33.77448653730064 29.070951628288487 19.786458501338664 15 15.871377421004853 30.87642334118254 31.548291068951695 21.703908168860913 16 16.228047943153907 29.189841802338172 30.728962660190685 23.853147594317235 17 15.666775726128652 27.79772081084942 28.586174639527755 27.949328823494174 18 20.87167695051312 27.15396277539434 26.261364840765506 25.712995433327034 19 21.854133737621368 31.152912118042273 25.651246273161693 21.341707871174663 20 23.794163321920493 29.600427635974874 24.442990318284664 22.162418723819968 21 21.584096365555027 30.984253964157837 25.767371559442783 21.66427811084435 22 19.38877547728875 29.591211343412887 24.307050002995297 26.712963176303067 23 19.954655840595002 31.483316206389656 22.472546968530967 26.08948098448437 24 17.174100374642293 31.16673655688526 27.320316856138284 24.338846212334165 25 20.35556456704162 29.106895169280257 25.11163234365712 25.42590792002101 26 22.79189150580396 31.281479399282052 22.630145571341018 23.296483523572974 27 18.970816609602455 29.59075052878479 24.439764615887967 26.998668245724794 28 23.904298018036286 27.99541028630413 27.057652518121532 21.042639177538053 29 21.53340675646408 30.326210675231675 25.440193173492098 22.70018939481215 30 23.36099757150691 28.65207113134599 23.62642679729225 24.360504499854844 31 28.057620261097565 30.128521199776966 19.333938536544903 22.47992000258056 32 25.10610256811992 30.774122493744443 20.209947144562157 23.90982779357348 33 26.24569714341012 28.47834401655246 22.196979820927435 23.078979019109983 34 22.506186436382237 31.289774062587846 22.319095697373818 23.884943803656103 35 23.76421037109402 29.134083232338128 23.735179049523747 23.366527347044105 36 26.89544576903049 28.861280972503188 24.02134493357357 20.221928324892747 37 22.01311478431571 33.768035132507244 20.963379061504927 23.25547102167211 38 23.488643223490485 32.385130433580485 22.908016792085046 21.218209550843984 39 21.267516716050636 30.705921928785706 24.097840161838096 23.92872119332556 40 23.553157271424425 30.65062417341376 22.208039372001824 23.58817918315999 41 20.90024745745529 26.720336210352663 27.337827812006065 25.041588520185986 42 25.335127438285397 28.483873792089653 22.05919624712567 24.121802522499276 43 22.361490643158977 26.87931725704701 25.516688401756625 25.24250369803739 44 22.35964738464658 25.154488104070378 25.060481919938066 27.42538259134498 45 24.803347357458517 27.6087868133286 24.68491799803693 22.90294783117595 46 21.025589036298367 28.312450750436625 28.57142857142857 22.09053164183644 47 21.783168284894035 28.452999212006986 24.59966729183851 25.164165211260464 48 22.017722930596708 25.329136848120104 30.57965872068643 22.073481500596756 49 22.404807218200336 24.54943849737566 29.266337030602703 23.779417253821304 50 20.812692678116374 24.855419410433765 27.215251120931583 27.116636790518278 51 20.738040708364245 24.836526010681684 29.808715847875877 24.616717433078193 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2509.0 1 1964.0 2 1419.0 3 953.0 4 487.0 5 577.5 6 668.0 7 599.0 8 530.0 9 527.5 10 525.0 11 529.0 12 533.0 13 526.0 14 519.0 15 523.0 16 527.0 17 554.5 18 582.0 19 543.0 20 504.0 21 557.5 22 611.0 23 735.5 24 860.0 25 1008.5 26 1468.5 27 1780.0 28 2001.5 29 2223.0 30 2549.0 31 2875.0 32 3030.0 33 3185.0 34 3555.5 35 3926.0 36 4695.0 37 5464.0 38 5771.5 39 6079.0 40 6900.5 41 7722.0 42 9713.5 43 11705.0 44 15693.0 45 19681.0 46 20854.0 47 22027.0 48 22234.5 49 22442.0 50 21035.0 51 19628.0 52 18303.0 53 16978.0 54 14516.0 55 12054.0 56 11164.0 57 10274.0 58 9229.0 59 8184.0 60 7517.5 61 6851.0 62 6464.5 63 6078.0 64 5475.0 65 4872.0 66 4072.0 67 3272.0 68 2880.5 69 2489.0 70 2171.0 71 1853.0 72 1706.0 73 1559.0 74 1245.5 75 845.0 76 758.0 77 590.0 78 422.0 79 283.5 80 145.0 81 109.5 82 74.0 83 49.5 84 25.0 85 17.5 86 10.0 87 8.0 88 6.0 89 3.5 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 217007.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.5711060011889 #Duplication Level Percentage of deduplicated Percentage of total 1 80.52973644596307 31.061210007050466 2 7.489665718859764 5.777693807112213 3 2.5184583403025016 2.914191708101582 4 1.339275047191226 2.066292792398402 5 0.8589997849513751 1.6566285880178981 6 0.6535088767293493 1.5123936094227375 7 0.5400109913741606 1.45801748330699 8 0.4157606747747963 1.2829079246291595 9 0.44204439559389264 1.5345127115715163 >10 5.0285536785262 40.51712617565332 >50 0.13619746242622638 3.309570659011 >100 0.03942558122864447 2.8464519577709475 >500 0.0023894291653723926 0.5257894906615916 >1k 0.005973572913430981 3.53721308529218 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2179 1.004115074628929 No Hit CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT 1926 0.8875289737197417 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCC 1232 0.567723621818651 No Hit AATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCT 1199 0.5525167390913658 No Hit AATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGT 1140 0.525328676033492 No Hit AAACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCT 623 0.2870875133060224 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 518 0.2387019773555692 No Hit AAAAACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTT 434 0.1999935485952066 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 406 0.18709073900841908 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 337 0.15529452966954982 No Hit AACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTG 302 0.13916601768606543 No Hit CCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC 291 0.13409705677697034 No Hit AAAAAACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCT 258 0.11889017404968502 No Hit GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 256 0.11796854479348594 No Hit AATGATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCT 254 0.11704691553728681 No Hit AATGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTT 252 0.1161252862810877 No Hit AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTAT 222 0.10230084743810108 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.608146280995544E-4 0.0 0.0 1.1709299700009677 0.0 2 4.608146280995544E-4 0.0 0.0 1.585202320662467 0.0 3 4.608146280995544E-4 0.0 0.0 2.134493357357136 0.0 4 4.608146280995544E-4 0.0 0.0 4.5228955747971265 0.0 5 4.608146280995544E-4 0.0 0.0 4.998917085623966 0.0 6 4.608146280995544E-4 0.0 0.0 6.813605091080011 0.0 7 4.608146280995544E-4 0.0 0.0 8.22093296529605 0.0 8 4.608146280995544E-4 0.0 0.0 9.295552678024212 0.0 9 4.608146280995544E-4 0.0 0.0 10.989968065546273 0.0 10 4.608146280995544E-4 0.0 0.0 12.836452280341188 0.0 11 4.608146280995544E-4 0.0 0.0 15.12854424050837 0.0 12 4.608146280995544E-4 0.0 0.0 16.087038666955443 0.0 13 4.608146280995544E-4 0.0 0.0 16.559373660757487 0.0 14 4.608146280995544E-4 0.0 0.0 17.11557691687365 0.0 15 9.216292561991088E-4 0.0 0.0 17.458423000179717 0.0 16 9.216292561991088E-4 0.0 0.0 18.08605252365131 0.0 17 9.216292561991088E-4 0.0 0.0 18.8210518554701 0.0 18 9.216292561991088E-4 0.0 0.0 19.97032353795039 0.0 19 9.216292561991088E-4 0.0 0.0 20.40302847373587 0.0 20 9.216292561991088E-4 0.0 0.0 20.81776163902547 0.0 21 9.216292561991088E-4 0.0 0.0 21.27995871100932 0.0 22 9.216292561991088E-4 0.0 0.0 21.708055500513808 0.0 23 9.216292561991088E-4 0.0 0.0 22.179929679687753 0.0 24 9.216292561991088E-4 0.0 0.0 22.498352587704545 0.0 25 9.216292561991088E-4 0.0 0.0 22.79281313506016 0.0 26 9.216292561991088E-4 0.0 0.0 23.056399102333103 0.0 27 9.216292561991088E-4 0.0 0.0 23.357771869110213 0.0 28 9.216292561991088E-4 0.0 0.0 23.648085084812934 0.0 29 9.216292561991088E-4 0.0 0.0 23.962360661176827 0.0 30 9.216292561991088E-4 0.0 0.0 24.371103236301135 0.0 31 9.216292561991088E-4 0.0 0.0 24.64252305225177 0.0 32 9.216292561991088E-4 0.0 0.0 24.916707755971007 0.0 33 9.216292561991088E-4 0.0 0.0 25.21116830332662 0.0 34 9.216292561991088E-4 0.0 0.0 25.48166649002106 0.0 35 9.216292561991088E-4 0.0 0.0 25.81391383688084 0.0 36 9.216292561991088E-4 0.0 0.0 26.073813287128985 0.0 37 9.216292561991088E-4 0.0 0.0 26.33924251291433 0.0 38 9.216292561991088E-4 0.0 0.0 26.609740699608768 0.0 39 9.216292561991088E-4 0.0 0.0 26.892680881261896 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTAGCG 20 7.02063E-4 45.0 1 TCCGCGG 20 7.02063E-4 45.0 2 ACGTTAG 20 7.02063E-4 45.0 1 ATGGCAG 25 3.8803177E-5 45.0 1 GAACTAT 20 7.02063E-4 45.0 15 CAACCGG 35 1.2058445E-7 45.0 2 TGAACGG 20 7.02063E-4 45.0 2 ATTCGGG 25 3.8803177E-5 45.0 3 TGAGTTA 20 7.02063E-4 45.0 10 TAACGTG 25 3.8803177E-5 45.0 1 ACGGGCG 35 1.2058445E-7 45.0 5 ATCCGCG 20 7.02063E-4 45.0 1 GTTACGG 40 6.7702786E-9 45.0 2 AACGGGC 25 3.8803177E-5 45.0 4 CTAACGG 25 3.8803177E-5 45.0 2 TCTACGA 25 3.8803177E-5 45.0 22 TACGACG 20 7.02063E-4 45.0 1 TCTAAGG 20 7.02063E-4 45.0 2 ACTACGG 20 7.02063E-4 45.0 2 TAGGGTC 40 6.7702786E-9 45.0 5 >>END_MODULE