SRR3548662_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548662_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	320653
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4215	1.3145050880546885	No Hit
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	3944	1.2299900515510536	TruSeq Adapter, Index 14 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCC	3852	1.2012986000442847	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGT	3463	1.0799836583471853	No Hit
AATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	3035	0.9465060361200425	No Hit
AAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	1214	0.378602414448017	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	957	0.29845346839106446	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	831	0.25915865437092434	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTT	796	0.24824342825421875	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTT	782	0.24387733780753648	No Hit
AACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	702	0.21892824954078083	No Hit
TGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTC	699	0.2179926587307775	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT	581	0.1811927535373129	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTAT	542	0.16903007300726955	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATCGACTC	473	0.1475114843771928	No Hit
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	464	0.14470471194718276	TruSeq Adapter, Index 21 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT	432	0.13472507664048053	No Hit
TGCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGAC	410	0.1278640773671227	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	394	0.1228742597137716	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	385	0.12006748728376157	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	383	0.11944376007709268	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT	322	0.1004200802736915	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCC	25	3.884494E-5	45.0	6
GTACCTC	25	3.884494E-5	45.0	18
TCGCAGC	20	7.025672E-4	45.0	39
CGAACAT	20	7.025672E-4	45.0	21
ATCTAGT	20	7.025672E-4	45.0	12
CGAAATT	20	7.025672E-4	45.0	43
TGCTTAA	20	7.025672E-4	45.0	38
CGAGTAG	20	7.025672E-4	45.0	1
ACGCATA	20	7.025672E-4	45.0	39
CTATCGG	25	3.884494E-5	45.0	2
TATGGGC	45	3.8380676E-10	45.0	4
CGTGAAC	25	3.884494E-5	45.0	34
GACCGAA	50	2.1827873E-11	45.0	9
GCCGGAT	20	7.025672E-4	45.0	15
CCTACGC	20	7.025672E-4	45.0	36
ATTGTCG	20	7.025672E-4	45.0	35
GTTAGTA	20	7.025672E-4	45.0	13
CTAAGTG	20	7.025672E-4	45.0	41
TTCAACG	25	3.884494E-5	45.0	1
CACGAAC	20	7.025672E-4	45.0	19

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content