##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548662_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	320653
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.071934458745122	31.0	31.0	33.0	28.0	34.0
2	31.350587706960482	31.0	31.0	34.0	30.0	34.0
3	31.54268321207037	31.0	31.0	34.0	30.0	34.0
4	35.47403579570439	37.0	35.0	37.0	33.0	37.0
5	34.80991289649559	35.0	35.0	37.0	32.0	37.0
6	34.8962492164427	35.0	35.0	37.0	32.0	37.0
7	34.778436503011044	35.0	35.0	37.0	32.0	37.0
8	35.23277499352883	37.0	35.0	37.0	33.0	37.0
9	36.63812594923484	39.0	35.0	39.0	32.0	39.0
10	36.18253064839562	37.0	35.0	39.0	32.0	39.0
11	36.33726177519	38.0	35.0	39.0	32.0	39.0
12	35.88893289630847	37.0	35.0	39.0	31.0	39.0
13	35.48048825365738	37.0	35.0	39.0	30.0	39.0
14	36.4015243892931	38.0	35.0	40.0	31.0	41.0
15	36.56345020941641	38.0	35.0	40.0	31.0	41.0
16	36.46562483432246	38.0	35.0	40.0	31.0	41.0
17	36.41866441293236	38.0	35.0	40.0	31.0	41.0
18	36.28149744427777	38.0	35.0	40.0	31.0	41.0
19	36.301849663031376	38.0	35.0	40.0	30.0	41.0
20	36.21632106981691	38.0	34.0	40.0	30.0	41.0
21	36.29434310609911	38.0	34.0	40.0	31.0	41.0
22	36.26542087552588	38.0	35.0	40.0	30.0	41.0
23	36.193392857699756	38.0	35.0	40.0	31.0	41.0
24	35.795763644812304	38.0	34.0	40.0	29.0	41.0
25	35.9063130549223	38.0	34.0	40.0	30.0	41.0
26	35.85053624946593	38.0	34.0	40.0	30.0	41.0
27	35.63867171054068	38.0	34.0	40.0	29.0	41.0
28	35.606752470739394	38.0	34.0	40.0	29.0	41.0
29	35.209375867370646	37.0	34.0	40.0	27.0	41.0
30	35.2519078255934	37.0	34.0	40.0	27.0	41.0
31	35.0746539093662	37.0	34.0	40.0	27.0	41.0
32	35.03062188721141	37.0	34.0	40.0	27.0	41.0
33	34.9178270591574	37.0	34.0	40.0	26.0	41.0
34	34.85244485471834	37.0	34.0	40.0	26.0	41.0
35	34.80225664503373	37.0	33.0	40.0	25.0	41.0
36	34.80850639164455	37.0	33.0	40.0	26.0	41.0
37	34.647478738698844	37.0	33.0	40.0	25.0	41.0
38	34.55089458074617	37.0	33.0	40.0	25.0	41.0
39	34.435358471618855	37.0	33.0	40.0	24.0	41.0
40	34.19791176131207	36.0	33.0	40.0	23.0	41.0
41	34.28574814519122	36.0	33.0	40.0	24.0	41.0
42	34.25520422388065	36.0	33.0	40.0	24.0	41.0
43	34.26420148883684	36.0	33.0	40.0	24.0	41.0
44	34.16203809102051	36.0	33.0	39.0	24.0	41.0
45	33.8790031591783	36.0	33.0	39.0	23.0	41.0
46	33.753687007450424	35.0	33.0	39.0	23.0	40.0
47	33.631667254009784	35.0	33.0	39.0	23.0	40.0
48	33.68977056194703	35.0	33.0	39.0	23.0	40.0
49	33.64097638256932	35.0	33.0	39.0	23.0	40.0
50	33.49634963652297	35.0	32.0	39.0	23.0	40.0
51	32.114793873751374	35.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	8.0
11	9.0
12	9.0
13	13.0
14	24.0
15	43.0
16	76.0
17	148.0
18	360.0
19	590.0
20	1048.0
21	1566.0
22	2139.0
23	2578.0
24	2968.0
25	3310.0
26	3661.0
27	4429.0
28	5463.0
29	6918.0
30	9119.0
31	12049.0
32	16124.0
33	22771.0
34	35242.0
35	36302.0
36	35151.0
37	44074.0
38	47971.0
39	26487.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.154515940908084	37.0381689864121	20.57520122999005	24.232113842689763
2	22.767602361431205	35.96691750895828	18.613267301413053	22.65221282819746
3	18.884276772710688	33.14704680760823	22.499087798960247	25.46958862072084
4	21.7381406068242	32.0948190099578	18.722419562580107	27.444620820637887
5	16.434276304915283	37.2798632789963	18.502555722229324	27.783304693859094
6	18.575531805409586	37.207822786626046	23.32646193860653	20.890183469357844
7	72.26846466429443	12.981634352399634	8.407219018689986	6.342681964615955
8	74.45712343249556	8.164900998899121	6.786152008557537	10.591823560047779
9	71.08774906207022	10.255010868446576	12.410612094694265	6.246627974788947
10	39.35812233161705	28.50370961756166	14.173888907947221	17.964279142874073
11	31.506644254069037	27.27590261123395	23.612128999260882	17.605324135436128
12	30.277901656931324	23.683545764424473	29.672886266462502	16.365666312181702
13	25.085684525016138	33.23031438969852	22.36748135835311	19.31651972693223
14	15.187757482387504	36.629003938837315	29.65417445026243	18.529064128512754
15	13.467829709998036	30.69517515819281	33.21815170916848	22.618843422640676
16	14.835039747016246	29.270582218161067	33.926393952341	21.96798408248169
17	15.316869014167963	27.29617374545069	27.89869422709284	29.488263013288506
18	19.55696656510309	27.309272016790736	26.283552625423745	26.850208792682434
19	22.330057725952976	32.26977449142843	26.31972880341054	19.080438979208054
20	23.762447256068086	30.718876792046228	23.29839421430643	22.220281737579253
21	18.251193657941762	31.572447474372606	26.6733821295918	23.502976738093828
22	17.86105229017037	31.37784458589191	24.88921045491544	25.87189266902228
23	20.385276295559372	30.92221186142029	21.776811693637672	26.915700149382666
24	16.137694018144224	31.5705762927526	27.741826834615612	24.549902854487563
25	17.315290984335093	29.87060779097654	26.596975546774864	26.217125677913504
26	22.436403214690024	31.99252774806411	22.125787065768915	23.445281971476955
27	15.771254284226252	33.947288813764416	25.267812869363453	25.01364403264588
28	18.84311077707054	31.88524666851706	28.012212578706574	21.259429975705828
29	20.865858108297754	31.225655147464703	25.84476053553218	22.06372620870536
30	22.16695306140906	32.479346832869176	23.03455760588549	22.31914249983627
31	24.82621400704188	32.35179461910539	22.047197437728634	20.774793936124098
32	24.724234608751516	28.142883428503712	20.712109351853872	26.420772610890904
33	25.295568730060218	30.75598856084303	22.92945957156178	21.01898313753497
34	19.162459106885013	31.59521351741602	22.71614486688102	26.526182508817943
35	21.463388772286553	29.91395683184003	23.213879177802795	25.408775218070623
36	25.635500057694767	28.132591929593676	26.715483716041955	19.51642429666961
37	19.614973195323294	32.08078514780775	22.4760098923135	25.828231764555454
38	22.32818654433297	28.389879402344594	26.359959208240685	22.921974845081756
39	21.257246930482484	26.744487031152055	25.373846494497165	26.624419543868292
40	24.959067902062355	27.16550289565356	21.150901441745436	26.724527760538653
41	18.562433534069537	25.164897880263087	28.192157877830553	28.08051070783682
42	24.202174936769655	26.839293566565726	22.28608495788282	26.672446538781795
43	21.967048491671683	25.15304706333638	26.880147698602542	25.999756746389398
44	21.928689268461547	25.70878800447836	24.77506837609503	27.587454350965064
45	24.76508874078833	28.236130645900708	24.82465468902521	22.174125924285754
46	19.498648071279547	26.627850043504974	30.577602579735725	23.295899305479757
47	21.84386236835458	27.609284803198474	24.004453412255618	26.542399416191337
48	21.483971770106628	23.9626636894088	32.01560565471085	22.53775888577372
49	23.21200799618279	20.909830876367913	29.372249752848095	26.505911374601204
50	19.59220715227988	24.355923693213537	26.38646761452411	29.665401539982472
51	18.473864270722558	24.135747989259418	31.212868739727995	26.177519000290033
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4725.0
1	3719.5
2	2714.0
3	1825.0
4	936.0
5	1030.0
6	1124.0
7	1032.0
8	940.0
9	875.0
10	810.0
11	820.0
12	830.0
13	850.5
14	871.0
15	917.0
16	963.0
17	988.0
18	1013.0
19	985.0
20	957.0
21	1049.0
22	1141.0
23	1093.5
24	1046.0
25	1384.0
26	2020.0
27	2318.0
28	2485.5
29	2653.0
30	2870.5
31	3088.0
32	3293.5
33	3499.0
34	4713.0
35	5927.0
36	6332.5
37	6738.0
38	6823.0
39	6908.0
40	8752.5
41	10597.0
42	14036.5
43	17476.0
44	25927.5
45	34379.0
46	35102.5
47	35826.0
48	36508.5
49	37191.0
50	34668.5
51	32146.0
52	28347.0
53	24548.0
54	21500.5
55	18453.0
56	15782.5
57	13112.0
58	11777.5
59	10443.0
60	10337.0
61	10231.0
62	9002.0
63	7773.0
64	6670.5
65	5568.0
66	4416.0
67	3264.0
68	2979.5
69	2695.0
70	2266.5
71	1838.0
72	1635.0
73	1432.0
74	1205.0
75	938.5
76	899.0
77	698.0
78	497.0
79	300.0
80	103.0
81	166.5
82	230.0
83	129.0
84	28.0
85	17.0
86	6.0
87	6.5
88	7.0
89	4.5
90	2.0
91	3.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	320653.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.184436248691554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.13055057380572	25.145877240178216
2	9.615801808217793	6.18958320553277
3	3.3571742344820152	3.2414628037630893
4	1.5884530713412923	2.0449386643448846
5	0.960961063085	1.5463995036167022
6	0.6093343848339361	1.17666501976941
7	0.4704855062731684	1.0599617547807507
8	0.3636023424373068	0.9361869128038734
9	0.3066646090579249	0.8882844803959231
>10	3.6979556385868224	28.010699170454945
>50	0.8021227384520558	16.887371377621985
>100	0.08290932414884263	4.638175960724801
>500	0.00799126015892459	1.893592320556532
>1k	0.0059934451191934425	6.3408015854561075
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4215	1.3145050880546885	No Hit
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	3944	1.2299900515510536	TruSeq Adapter, Index 14 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCC	3852	1.2012986000442847	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGT	3463	1.0799836583471853	No Hit
AATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	3035	0.9465060361200425	No Hit
AAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	1214	0.378602414448017	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	957	0.29845346839106446	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	831	0.25915865437092434	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTT	796	0.24824342825421875	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTT	782	0.24387733780753648	No Hit
AACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	702	0.21892824954078083	No Hit
TGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTC	699	0.2179926587307775	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT	581	0.1811927535373129	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTAT	542	0.16903007300726955	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATCGACTC	473	0.1475114843771928	No Hit
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	464	0.14470471194718276	TruSeq Adapter, Index 21 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT	432	0.13472507664048053	No Hit
TGCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGAC	410	0.1278640773671227	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	394	0.1228742597137716	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	385	0.12006748728376157	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	383	0.11944376007709268	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT	322	0.1004200802736915	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5056774768987036	0.0
2	0.0	0.0	0.0	1.9008086623234461	0.0
3	0.0	0.0	0.0	2.5522917296891032	0.0
4	0.0	0.0	0.0	5.735795392527124	0.0
5	0.0	0.0	0.0	6.2141941600421635	0.0
6	0.0	0.0	0.0	8.4580527860335	0.0
7	0.0	0.0	0.0	10.110929883706062	0.0
8	0.0	0.0	0.0	11.221476175180022	0.0
9	0.0	0.0	0.0	13.676154597025445	0.0
10	0.0	0.0	0.0	15.65898338702585	0.0
11	0.0	0.0	0.0	18.957564719494282	0.0
12	0.0	0.0	0.0	20.10241600733503	0.0
13	0.0	0.0	0.0	20.626658724540235	0.0
14	0.0	0.0	0.0	21.283131609559245	0.0
15	0.0	0.0	0.0	21.67452043174397	0.0
16	0.0	0.0	0.0	22.52684365965701	0.0
17	0.0	0.0	0.0	23.3922651589101	0.0
18	0.0	0.0	0.0	25.084125206999467	0.0
19	0.0	0.0	0.0	25.601818788534647	0.0
20	0.0	0.0	0.0	26.216190087103506	0.0
21	0.0	0.0	0.0	26.71517185243862	0.0
22	0.0	0.0	0.0	27.187645211490302	0.0
23	0.0	0.0	0.0	27.668227024228685	0.0
24	0.0	0.0	0.0	27.981961809183137	3.1186360333444567E-4
25	0.0	0.0	0.0	28.260456006960794	3.1186360333444567E-4
26	0.0	0.0	0.0	28.53084175105176	3.1186360333444567E-4
27	0.0	0.0	0.0	28.90258316622642	3.1186360333444567E-4
28	0.0	0.0	0.0	29.23440604017427	3.1186360333444567E-4
29	0.0	0.0	0.0	29.534418826582005	3.1186360333444567E-4
30	0.0	0.0	0.0	30.07924454160728	3.1186360333444567E-4
31	0.0	0.0	0.0	30.369277692708316	3.1186360333444567E-4
32	3.1186360333444567E-4	0.0	0.0	30.707337838722857	3.1186360333444567E-4
33	3.1186360333444567E-4	0.0	0.0	31.006726897923922	3.1186360333444567E-4
34	3.1186360333444567E-4	0.0	0.0	31.293641412991615	3.1186360333444567E-4
35	3.1186360333444567E-4	0.0	0.0	31.597084699036028	3.1186360333444567E-4
36	3.1186360333444567E-4	0.0	0.0	31.849070490530263	3.1186360333444567E-4
37	3.1186360333444567E-4	0.0	0.0	32.14845954973133	3.1186360333444567E-4
38	3.1186360333444567E-4	0.0	0.0	32.425394429492314	3.1186360333444567E-4
39	3.1186360333444567E-4	0.0	0.0	32.743183441290114	3.1186360333444567E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCC	25	3.884494E-5	45.0	6
GTACCTC	25	3.884494E-5	45.0	18
TCGCAGC	20	7.025672E-4	45.0	39
CGAACAT	20	7.025672E-4	45.0	21
ATCTAGT	20	7.025672E-4	45.0	12
CGAAATT	20	7.025672E-4	45.0	43
TGCTTAA	20	7.025672E-4	45.0	38
CGAGTAG	20	7.025672E-4	45.0	1
ACGCATA	20	7.025672E-4	45.0	39
CTATCGG	25	3.884494E-5	45.0	2
TATGGGC	45	3.8380676E-10	45.0	4
CGTGAAC	25	3.884494E-5	45.0	34
GACCGAA	50	2.1827873E-11	45.0	9
GCCGGAT	20	7.025672E-4	45.0	15
CCTACGC	20	7.025672E-4	45.0	36
ATTGTCG	20	7.025672E-4	45.0	35
GTTAGTA	20	7.025672E-4	45.0	13
CTAAGTG	20	7.025672E-4	45.0	41
TTCAACG	25	3.884494E-5	45.0	1
CACGAAC	20	7.025672E-4	45.0	19
>>END_MODULE