SRR3548661_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548661_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	326074
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4375	1.3417199776737796	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCC	3927	1.2043278519599845	No Hit
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	3906	1.1978875960671505	TruSeq Adapter, Index 14 (95% over 23bp)
AATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGT	3552	1.0893232824450891	No Hit
AATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	3232	0.9911860497923783	No Hit
AAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	1342	0.4115630194373056	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	979	0.3002385961468869	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	925	0.28367793813674197	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTT	842	0.2582235934174451	No Hit
TGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTC	800	0.24534308163177684	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTT	792	0.24288965081545907	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT	666	0.20424811545845423	No Hit
AACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	659	0.20210136349417618	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATCGACTC	526	0.16131307617289328	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTAT	504	0.15456614142801942	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT	484	0.148432564387225	No Hit
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	466	0.14291234505051	TruSeq Adapter, Index 21 (95% over 21bp)
TGCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGAC	418	0.1281917601526034	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	386	0.11837803688733234	No Hit
TGCCCAGGGAATGATCTGTCTCTTATACACATCTGACGCAGATCGACTCGT	383	0.11745800033121315	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	379	0.11623128492305429	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	358	0.10979102903022012	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGAATA	65	0.0	45.000004	17
AACGGGA	210	0.0	45.000004	4
CGGGCAA	30	2.1607011E-6	45.000004	6
TGGGACG	30	2.1607011E-6	45.000004	6
GCATGAG	30	2.1607011E-6	45.000004	9
TGTGTAC	30	2.1607011E-6	45.000004	13
TATAACG	30	2.1607011E-6	45.000004	1
ATACCCG	30	2.1607011E-6	45.000004	1
ACGACAG	30	2.1607011E-6	45.000004	1
AACGTTG	35	1.2083547E-7	45.0	1
TATTTAC	20	7.025846E-4	45.0	45
TTCTACT	25	3.8846396E-5	45.0	19
TTCTACG	20	7.025846E-4	45.0	1
TGTCACG	20	7.025846E-4	45.0	1
AACCGTA	20	7.025846E-4	45.0	15
TAATAGT	20	7.025846E-4	45.0	39
CTCACGG	20	7.025846E-4	45.0	2
GTACCGG	25	3.8846396E-5	45.0	2
AGTCCAT	20	7.025846E-4	45.0	40
GCGACGT	25	3.8846396E-5	45.0	10

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content