##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548661_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	326074
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.144123726516067	31.0	31.0	33.0	28.0	34.0
2	31.43095432325178	31.0	31.0	34.0	30.0	34.0
3	31.641017069744905	31.0	31.0	34.0	30.0	34.0
4	35.530649484472846	37.0	35.0	37.0	33.0	37.0
5	34.85273588203905	35.0	35.0	37.0	32.0	37.0
6	34.917224311045956	35.0	35.0	37.0	32.0	37.0
7	34.825677606923584	35.0	35.0	37.0	32.0	37.0
8	35.22845734403847	37.0	35.0	37.0	32.0	37.0
9	36.716512202751524	39.0	37.0	39.0	32.0	39.0
10	36.2310027785104	38.0	35.0	39.0	32.0	39.0
11	36.34268908284623	38.0	35.0	39.0	32.0	39.0
12	35.87176837159663	37.0	35.0	39.0	31.0	39.0
13	35.55295730417022	37.0	35.0	39.0	30.0	39.0
14	36.46047522954912	38.0	35.0	40.0	31.0	41.0
15	36.603188233345804	38.0	35.0	40.0	31.0	41.0
16	36.50159166324209	38.0	35.0	40.0	31.0	41.0
17	36.47328827198734	38.0	35.0	40.0	31.0	41.0
18	36.29300404202727	38.0	35.0	40.0	31.0	41.0
19	36.33939841876384	38.0	35.0	40.0	31.0	41.0
20	36.23798892275986	38.0	34.0	40.0	30.0	41.0
21	36.308794936118794	38.0	34.0	40.0	31.0	41.0
22	36.289204291050495	38.0	35.0	40.0	31.0	41.0
23	36.18043450259757	38.0	35.0	40.0	30.0	41.0
24	35.79653698240277	38.0	34.0	40.0	29.0	41.0
25	35.864454080975484	38.0	34.0	40.0	30.0	41.0
26	35.78273030048394	38.0	34.0	40.0	30.0	41.0
27	35.50058268981888	38.0	34.0	40.0	29.0	41.0
28	35.43288026644259	37.0	34.0	40.0	27.0	41.0
29	35.16423879242135	37.0	34.0	40.0	27.0	41.0
30	35.22482013285328	37.0	34.0	40.0	27.0	41.0
31	35.05731827744622	37.0	34.0	40.0	27.0	41.0
32	34.981970350288584	37.0	34.0	40.0	27.0	41.0
33	34.865012236486194	37.0	34.0	40.0	26.0	41.0
34	34.782665897925014	37.0	34.0	40.0	25.0	41.0
35	34.70926844826634	37.0	33.0	40.0	25.0	41.0
36	34.68172868735318	37.0	33.0	40.0	25.0	41.0
37	34.54170525708888	37.0	33.0	40.0	25.0	41.0
38	34.5076823052436	37.0	33.0	40.0	25.0	41.0
39	34.40559811576513	37.0	33.0	40.0	24.0	41.0
40	34.113707317970764	36.0	33.0	40.0	23.0	41.0
41	34.21211136122475	36.0	33.0	40.0	24.0	41.0
42	34.183105675398835	36.0	33.0	40.0	24.0	41.0
43	34.220367155921664	36.0	33.0	40.0	24.0	41.0
44	34.03099909836418	36.0	33.0	39.0	23.0	41.0
45	33.74499346774045	35.0	33.0	39.0	23.0	41.0
46	33.60588087366671	35.0	33.0	39.0	23.0	40.0
47	33.4901218741758	35.0	32.0	39.0	23.0	40.0
48	33.5370774732116	35.0	32.0	39.0	23.0	40.0
49	33.446895489980804	35.0	32.0	39.0	23.0	40.0
50	33.34076620644394	35.0	32.0	39.0	22.0	40.0
51	31.876285751087178	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	3.0
11	6.0
12	8.0
13	7.0
14	21.0
15	46.0
16	87.0
17	159.0
18	329.0
19	607.0
20	1004.0
21	1630.0
22	2198.0
23	2638.0
24	2953.0
25	3310.0
26	3935.0
27	4648.0
28	5809.0
29	7413.0
30	9453.0
31	12490.0
32	16537.0
33	23262.0
34	36121.0
35	37119.0
36	35026.0
37	43720.0
38	48212.0
39	27317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.97628759116029	37.297055269662714	20.5134417340849	24.213215405092097
2	22.608364972368236	36.33715046277839	18.608352705214156	22.446131859639223
3	18.85492250225409	33.20963952967732	22.568803400455113	25.366634567613488
4	21.717156228340805	31.960229886467488	18.63288701337733	27.689726871814376
5	16.331262228819224	37.26945417297914	18.57952489312242	27.819758705079217
6	18.698822966565874	37.05079215147482	23.43854462483976	20.81184025711955
7	71.97998000453885	13.11328103436643	8.551126431423542	6.355612529671179
8	74.17242711777081	8.188325349460552	6.8024436170930525	10.836803915675583
9	70.83177438250213	10.328637057845764	12.575979685592841	6.263608874059262
10	39.39443193876237	28.59013598140299	14.129001392321989	17.88643068751265
11	31.570134386672965	27.297177941203532	23.557536019431172	17.575151652692334
12	30.05820764611714	23.972165827388874	29.6803793004042	16.289247226089785
13	24.92409698412017	33.53011893005882	22.158467096425966	19.387316989395046
14	15.162509123695846	36.569613032624495	29.901188073872802	18.366689769806854
15	13.309555499671855	30.94880303244049	33.2651484018965	22.476493065991153
16	14.750332746554463	29.1970534295896	33.995964106307156	22.056649717548776
17	15.306341505302479	27.65108533645737	27.853800057655626	29.18877310058453
18	19.57868459306783	27.438556891993844	26.20141440286561	26.78134411207272
19	22.182081368033025	32.48710415427173	26.165839656029	19.16497482166625
20	23.807479283843545	30.79454356986451	23.339180676778888	22.058796469513055
21	18.1176665419506	31.64864417279513	26.546734790262335	23.686954494991934
22	17.849629225267886	31.32325791078099	25.004753522206613	25.822359341744512
23	20.56833724860001	30.852812551752056	21.879389341069817	26.699460858578117
24	16.142961413666836	31.77959604261609	27.875574256150443	24.201868287566626
25	17.267552764096493	29.863159896219877	26.558388586639843	26.310898753043787
26	22.475573029435036	32.03475284751314	22.071063623594643	23.41861049945718
27	15.94975373688181	33.84354471684342	25.224642259119097	24.982059287155675
28	18.600992412765198	31.975563829069475	28.058968209670198	21.364475548495125
29	20.933285082527277	31.19935965455694	25.787091273759945	22.080263989155835
30	22.345847875022233	32.247894649680745	23.06715653501966	22.33910094027736
31	24.889748952691722	32.325177720394755	22.177787864104467	20.607285462809056
32	24.634285468942632	28.342032790102863	20.59961849150806	26.424063249446444
33	25.465998515674354	30.773382729073766	22.807706226194053	20.95291252905782
34	19.047210142482996	31.644964026570655	22.85401473285205	26.4538110980943
35	21.407717266632726	29.841999055429135	23.114078399381736	25.6362052785564
36	25.582536479449452	28.12030398007814	26.76969031569521	19.527469224777196
37	19.74398449431724	31.928028607003316	22.31517998981826	26.01280690886118
38	22.3811159430068	28.63644448806099	26.233922361181815	22.74851720775039
39	21.159307396480553	26.569735704165314	25.59265688156676	26.67830001778737
40	25.011807135803526	27.130651324545962	21.10993210130216	26.747609438348352
41	18.618473107331464	24.950471365395586	28.361046878929326	28.070008648343624
42	24.455185019351436	26.927629924495665	22.17625446984427	26.44093058630863
43	22.127799211221994	25.147359188405087	26.744542649827952	25.980298950544967
44	21.769598312039598	25.882468396744297	24.76983752154419	27.57809576967192
45	25.02591436299736	28.1647724136239	24.700528100983213	22.10878512239553
46	19.540656415414905	26.54213460748174	30.52803964744199	23.389169329661367
47	21.86558879272803	27.45082404607543	23.912363451241127	26.77122370995541
48	21.399130258775614	23.97676601016947	32.106822377742475	22.517281353312438
49	23.085250587290002	21.00903475898109	29.5058790335936	26.39983562013531
50	19.590951747149422	24.200641572158467	26.541827928629697	29.666578752062417
51	18.212123628378833	24.216895551316572	31.39931426608684	26.171666554217754
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4888.0
1	3864.0
2	2840.0
3	1856.5
4	873.0
5	975.0
6	1077.0
7	1000.0
8	923.0
9	891.0
10	859.0
11	853.0
12	847.0
13	861.0
14	875.0
15	949.5
16	1024.0
17	1035.0
18	1046.0
19	1032.0
20	1018.0
21	1042.0
22	1066.0
23	1082.0
24	1098.0
25	1411.0
26	2043.0
27	2362.0
28	2510.5
29	2659.0
30	2907.5
31	3156.0
32	3329.5
33	3503.0
34	4796.5
35	6090.0
36	6513.0
37	6936.0
38	7021.0
39	7106.0
40	8920.0
41	10734.0
42	14238.5
43	17743.0
44	26731.5
45	35720.0
46	36005.5
47	36291.0
48	37250.5
49	38210.0
50	35318.0
51	32426.0
52	28642.0
53	24858.0
54	21646.5
55	18435.0
56	15943.5
57	13452.0
58	11993.5
59	10535.0
60	10341.0
61	10147.0
62	8943.0
63	7739.0
64	6708.0
65	5677.0
66	4526.5
67	3376.0
68	3019.0
69	2662.0
70	2332.0
71	2002.0
72	1695.0
73	1388.0
74	1171.0
75	913.0
76	872.0
77	674.0
78	476.0
79	306.0
80	136.0
81	165.5
82	195.0
83	114.5
84	34.0
85	28.0
86	22.0
87	15.0
88	8.0
89	7.0
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	326074.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.801738937596085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.8536110475326	24.758802138827786
2	9.92581666969545	6.313164609441883
3	3.2938829253268715	3.1425361464675143
4	1.6038918194535556	2.040261957056319
5	0.9325947081287873	1.4829066721247657
6	0.6310343498020564	1.2040793791836413
7	0.4523065635349109	1.006889467730892
8	0.36044726934469346	0.9170279968375458
9	0.3095253429407903	0.8859099735695622
>10	3.724288782297532	28.00523350757117
>50	0.8117551688615804	17.01582200268687
>100	0.08586832044538242	4.64547055122992
>500	0.00898621958149351	2.1252313328353964
>1k	0.005990813054329007	6.456664264436718
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4375	1.3417199776737796	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCC	3927	1.2043278519599845	No Hit
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	3906	1.1978875960671505	TruSeq Adapter, Index 14 (95% over 23bp)
AATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGT	3552	1.0893232824450891	No Hit
AATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	3232	0.9911860497923783	No Hit
AAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	1342	0.4115630194373056	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	979	0.3002385961468869	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	925	0.28367793813674197	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTT	842	0.2582235934174451	No Hit
TGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTC	800	0.24534308163177684	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTT	792	0.24288965081545907	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT	666	0.20424811545845423	No Hit
AACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	659	0.20210136349417618	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATCGACTC	526	0.16131307617289328	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTAT	504	0.15456614142801942	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT	484	0.148432564387225	No Hit
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	466	0.14291234505051	TruSeq Adapter, Index 21 (95% over 21bp)
TGCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGAC	418	0.1281917601526034	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	386	0.11837803688733234	No Hit
TGCCCAGGGAATGATCTGTCTCTTATACACATCTGACGCAGATCGACTCGT	383	0.11745800033121315	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	379	0.11623128492305429	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	358	0.10979102903022012	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0667885203972107E-4	0.0	0.0	1.5263406466016916	0.0
2	3.0667885203972107E-4	0.0	0.0	1.9222630445849715	0.0
3	3.0667885203972107E-4	0.0	0.0	2.5478879027460026	0.0
4	3.0667885203972107E-4	0.0	0.0	5.886700564902445	0.0
5	3.0667885203972107E-4	0.0	0.0	6.374933297349681	0.0
6	3.0667885203972107E-4	0.0	0.0	8.618902457724321	0.0
7	3.0667885203972107E-4	0.0	0.0	10.321890123100891	0.0
8	3.0667885203972107E-4	0.0	0.0	11.494936732152825	0.0
9	3.0667885203972107E-4	0.0	0.0	13.937020430945124	0.0
10	3.0667885203972107E-4	0.0	0.0	15.830148984586321	0.0
11	3.0667885203972107E-4	0.0	0.0	19.115906205339893	0.0
12	3.0667885203972107E-4	0.0	0.0	20.269018689009243	0.0
13	3.0667885203972107E-4	0.0	0.0	20.81061354171139	0.0
14	3.0667885203972107E-4	0.0	0.0	21.446665480841773	0.0
15	3.0667885203972107E-4	0.0	0.0	21.8321607978557	0.0
16	3.0667885203972107E-4	0.0	0.0	22.6899415470108	0.0
17	3.0667885203972107E-4	0.0	0.0	23.53821525175267	0.0
18	3.0667885203972107E-4	0.0	0.0	25.257150217435306	0.0
19	3.0667885203972107E-4	0.0	0.0	25.762863644448807	0.0
20	3.0667885203972107E-4	0.0	0.0	26.380514852456805	0.0
21	3.0667885203972107E-4	0.0	0.0	26.855560394266334	0.0
22	3.0667885203972107E-4	0.0	0.0	27.32999257837178	0.0
23	3.0667885203972107E-4	0.0	0.0	27.83570600538528	0.0
24	3.0667885203972107E-4	0.0	0.0	28.149438471021917	0.0
25	3.0667885203972107E-4	0.0	0.0	28.42974294178622	0.0
26	3.0667885203972107E-4	0.0	0.0	28.683366352423068	0.0
27	3.0667885203972107E-4	0.0	0.0	29.046474113238098	0.0
28	3.0667885203972107E-4	0.0	0.0	29.370326980992044	0.0
29	3.0667885203972107E-4	0.0	0.0	29.679152584996043	0.0
30	3.0667885203972107E-4	0.0	0.0	30.175052288744272	0.0
31	3.0667885203972107E-4	0.0	0.0	30.46241037310549	0.0
32	3.0667885203972107E-4	0.0	0.0	30.786876598563516	0.0
33	3.0667885203972107E-4	0.0	0.0	31.061354171139065	0.0
34	3.0667885203972107E-4	0.0	0.0	31.322337874224868	0.0
35	3.0667885203972107E-4	0.0	0.0	31.61889632414728	0.0
36	3.0667885203972107E-4	0.0	0.0	31.887240319682036	0.0
37	3.0667885203972107E-4	0.0	0.0	32.173985046339176	0.0
38	3.0667885203972107E-4	0.0	0.0	32.4536761593994	0.0
39	3.0667885203972107E-4	0.0	0.0	32.7833559253421	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGAATA	65	0.0	45.000004	17
AACGGGA	210	0.0	45.000004	4
CGGGCAA	30	2.1607011E-6	45.000004	6
TGGGACG	30	2.1607011E-6	45.000004	6
GCATGAG	30	2.1607011E-6	45.000004	9
TGTGTAC	30	2.1607011E-6	45.000004	13
TATAACG	30	2.1607011E-6	45.000004	1
ATACCCG	30	2.1607011E-6	45.000004	1
ACGACAG	30	2.1607011E-6	45.000004	1
AACGTTG	35	1.2083547E-7	45.0	1
TATTTAC	20	7.025846E-4	45.0	45
TTCTACT	25	3.8846396E-5	45.0	19
TTCTACG	20	7.025846E-4	45.0	1
TGTCACG	20	7.025846E-4	45.0	1
AACCGTA	20	7.025846E-4	45.0	15
TAATAGT	20	7.025846E-4	45.0	39
CTCACGG	20	7.025846E-4	45.0	2
GTACCGG	25	3.8846396E-5	45.0	2
AGTCCAT	20	7.025846E-4	45.0	40
GCGACGT	25	3.8846396E-5	45.0	10
>>END_MODULE