SRR3548660_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548660_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	283188
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCC	10288	3.6329222989674705	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGT	8871	3.1325479893215817	No Hit
AATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	8481	2.9948302894190433	No Hit
CTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGCT	5365	1.894501179428507	TruSeq Adapter, Index 23 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4336	1.5311383250702713	No Hit
AATGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTT	2278	0.8044126163538003	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT	1884	0.6652824272214924	No Hit
AACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTG	1730	0.610901591875362	TruSeq Adapter, Index 22 (95% over 21bp)
AAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	1705	0.6020735341893019	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTAT	1604	0.5664081811376188	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATGGTTGTC	1358	0.47954009350678706	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTT	1065	0.37607525742616216	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	995	0.35135669590519375	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	776	0.27402291057530687	No Hit
AATGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC	762	0.26907919827111315	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT	621	0.21928895292173395	No Hit
CTGCCGGGGAGGGTCATTTCCACATTTCATTTACACACAGAACTAAACAGA	532	0.18786106755935986	No Hit
CCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	514	0.18150486602539656	TruSeq Adapter, Index 22 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTGGTTGTCGTATGCC	464	0.16384875065327625	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATG	454	0.1603175275788522	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGC	442	0.15608005988954335	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCCATGGTTGTCGTATGCCGT	436	0.1539613260448889	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	428	0.15113634758534966	No Hit
AATCTGTCTCTTATACCCATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	391	0.13807082220998065	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTA	369	0.13030213144624772	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	359	0.12677090837182367	No Hit
AATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCATGGTTGT	311	0.10982103761458818	No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	306	0.10805542607737616	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT	304	0.10734918146249135	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	30	2.159688E-6	45.000004	29
GTTGAGG	35	1.2075907E-7	45.000004	2
TTGATCG	30	2.159688E-6	45.000004	28
GTGCACA	20	7.024272E-4	45.000004	16
CATGGGC	35	1.2075907E-7	45.000004	4
CTAGAAG	20	7.024272E-4	45.000004	1
AGGTAAT	40	6.7830115E-9	45.000004	6
AACCGCG	20	7.024272E-4	45.000004	1
TCGTTGA	30	2.159688E-6	45.000004	24
TACCCTC	20	7.024272E-4	45.000004	43
ATCTCAG	20	7.024272E-4	45.000004	10
ACAAAAG	30	2.159688E-6	45.000004	1
CTATCCG	20	7.024272E-4	45.000004	1
TCCGAGG	20	7.024272E-4	45.000004	2
CACATTT	80	0.0	45.000004	21
CACATGG	30	2.159688E-6	45.000004	2
CGTTGGG	35	1.2075907E-7	45.000004	3
CGTTGAT	30	2.159688E-6	45.000004	25
TGGCGCG	20	7.024272E-4	45.000004	30
ACATTGG	20	7.024272E-4	45.000004	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content