##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548657_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 323053 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.10622405611463 31.0 31.0 33.0 28.0 34.0 2 31.403825378498265 31.0 31.0 34.0 30.0 34.0 3 31.573419841326345 31.0 31.0 34.0 30.0 34.0 4 35.48331388348042 37.0 35.0 37.0 33.0 37.0 5 34.776145090743626 35.0 35.0 37.0 32.0 37.0 6 34.905993753346976 35.0 35.0 37.0 32.0 37.0 7 34.79949729610931 35.0 35.0 37.0 32.0 37.0 8 35.20319885591528 37.0 35.0 37.0 32.0 37.0 9 36.76979938276382 39.0 37.0 39.0 32.0 39.0 10 36.18509346763534 37.0 35.0 39.0 32.0 39.0 11 36.36914995372276 38.0 35.0 39.0 32.0 39.0 12 36.02073963095839 37.0 35.0 39.0 32.0 39.0 13 35.80735668760234 37.0 35.0 39.0 30.0 39.0 14 36.79105595676251 38.0 35.0 40.0 31.0 41.0 15 36.81327212562645 38.0 35.0 40.0 32.0 41.0 16 36.689304850906815 38.0 35.0 40.0 31.0 41.0 17 36.61368258459138 38.0 35.0 40.0 31.0 41.0 18 36.46846183134037 38.0 35.0 40.0 31.0 41.0 19 36.50197026494104 38.0 35.0 40.0 31.0 41.0 20 36.45785676034583 38.0 35.0 40.0 31.0 41.0 21 36.63714003584551 38.0 35.0 40.0 31.0 41.0 22 36.59372610686172 38.0 35.0 40.0 31.0 41.0 23 36.50710254973642 38.0 35.0 40.0 31.0 41.0 24 36.046038885260316 38.0 34.0 40.0 30.0 41.0 25 36.294388227318734 38.0 35.0 40.0 30.0 41.0 26 36.14732567101993 38.0 35.0 40.0 30.0 41.0 27 36.05697517125673 38.0 34.0 40.0 30.0 41.0 28 36.06201459203289 38.0 35.0 40.0 30.0 41.0 29 35.69886055848421 38.0 34.0 40.0 29.0 41.0 30 35.87371422026726 38.0 34.0 40.0 30.0 41.0 31 35.796448260811694 38.0 34.0 40.0 29.0 41.0 32 35.78088425119098 38.0 34.0 40.0 29.0 41.0 33 35.7860351087902 38.0 34.0 40.0 29.0 41.0 34 35.77121710679052 38.0 35.0 40.0 29.0 41.0 35 35.710771916682404 38.0 35.0 40.0 29.0 41.0 36 35.730917217917806 38.0 35.0 40.0 29.0 41.0 37 35.56878902223474 38.0 34.0 40.0 29.0 41.0 38 35.51651896128499 38.0 34.0 40.0 28.0 41.0 39 35.438779395331416 38.0 34.0 40.0 28.0 41.0 40 35.126808913707656 38.0 34.0 40.0 26.0 41.0 41 35.29032387874435 38.0 34.0 40.0 27.0 41.0 42 35.23258722253005 38.0 34.0 40.0 27.0 41.0 43 35.24354208133031 38.0 34.0 40.0 27.0 41.0 44 35.1046236995168 37.0 34.0 40.0 27.0 41.0 45 34.8716309707695 37.0 34.0 40.0 26.0 41.0 46 34.832426877323535 37.0 34.0 40.0 26.0 41.0 47 34.71008781840751 37.0 33.0 40.0 26.0 41.0 48 34.79228176181617 37.0 34.0 40.0 26.0 41.0 49 34.67622959700111 37.0 34.0 40.0 26.0 41.0 50 34.60000371456077 37.0 33.0 40.0 26.0 41.0 51 33.203211237784515 35.0 31.0 39.0 22.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 3.0 10 4.0 11 9.0 12 7.0 13 11.0 14 18.0 15 42.0 16 67.0 17 126.0 18 251.0 19 501.0 20 797.0 21 1197.0 22 1584.0 23 1925.0 24 2266.0 25 2536.0 26 3026.0 27 3529.0 28 4519.0 29 5972.0 30 7799.0 31 10683.0 32 14449.0 33 20028.0 34 30477.0 35 33307.0 36 37040.0 37 49432.0 38 58217.0 39 33229.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 17.99859465784252 39.381463722670894 18.833442190600305 23.78649942888628 2 20.939288599703453 38.7221291862326 18.49665534757455 21.8419268664894 3 18.902471111551357 29.65581499010998 27.00609497512792 24.435618923210743 4 24.945751935440935 29.962575800255685 18.707147124465646 26.384525139837734 5 16.295158998678236 37.90461627039526 19.421890525703212 26.378334205223293 6 20.648314672824583 34.44419336765175 24.25143861843103 20.656053341092637 7 71.19141441187668 12.97650849860549 9.294140589934159 6.53793649958366 8 72.96264080506913 6.2026973902115135 7.312731966581334 13.521929838138014 9 70.49183880044451 9.335619851850934 12.715870151337397 7.456671196367159 10 35.23601390483914 30.694963365144417 15.440190928423508 18.628831801592927 11 26.97699758244003 24.736188798742003 27.551825861391166 20.7349877574268 12 27.19275165375341 22.643343352329186 33.30413275840187 16.859772235515535 13 23.466428109319523 31.05187074566712 24.545817559347846 20.93588358566551 14 15.441429115346397 31.57036151962681 33.40442589915587 19.583783465870923 15 13.745113030988723 30.59776569169765 30.013651010824848 25.643470266488784 16 15.449786877075896 26.579230033462004 34.12752706212293 23.843456027339165 17 15.405831241313345 27.196156667791353 29.475039699368217 27.922972391527086 18 17.857750895363917 27.1354855085698 27.640665773108434 27.36609782295784 19 19.28692815110833 31.84431037631596 30.14737519849684 18.721386274078867 20 21.127493011982555 31.41032585984343 25.770384426084885 21.691796702089132 21 18.43660328181444 29.22368775402179 27.531705323894222 24.80800364026955 22 17.02785611029769 30.863975880118744 26.369047803301626 25.73912020628194 23 20.644909658786638 28.69033873698743 22.627866015793074 28.03688558843286 24 15.082974001170088 34.29313456305931 26.696548244405715 23.927343191364884 25 18.22177785069323 26.29661386831263 28.813538335814865 26.668069945179273 26 22.671821651555625 30.96767403491068 22.567194856571522 23.79330945696217 27 16.050926628138416 28.115820004767016 28.737389840057205 27.095863527037363 28 19.991456510232066 26.240585910051912 32.24300656548616 21.524951014229863 29 20.949194095086565 28.43217676356511 27.146938737606526 23.471690403741803 30 20.712390846084077 25.620873355146063 25.92422915125382 27.74250664751604 31 24.33934988995614 27.36393099584279 26.07002566142398 22.2266934527771 32 21.744729193042627 23.058135971496938 24.064781939805542 31.13235289565489 33 21.84068867956651 22.371561322755092 28.81230014889198 26.975449848786422 34 18.877397826362856 23.19774154705265 30.206498624064782 27.718362002519708 35 19.02257524307157 20.83466180471935 32.40799497296109 27.734767979247987 36 24.064781939805542 21.439825663281255 32.6785388156123 21.8168535813009 37 18.03790709264424 25.267061441930583 26.913850049372705 29.781181416052476 38 20.153039903669058 23.56703079680424 31.531977725017256 24.747951574509447 39 19.12658294459423 21.64072149151997 31.019987432402736 28.212708131483073 40 20.240022535001998 22.92131631651772 28.793417798317922 28.04524335016236 41 18.545563731028654 19.14082209420745 37.01405032610748 25.299563848656415 42 22.952270989589945 22.309032883149204 29.447180493603213 25.291515633657635 43 20.43472742862626 22.66655935713335 30.09568089446623 26.803032319774157 44 20.125799791365502 20.941145880087788 28.588807409310547 30.344246919236163 45 24.64580115337112 24.591940022225454 27.998192247092586 22.764066577310842 46 17.920588881700525 22.77056705865601 35.62449505189551 23.684349007747954 47 21.03277171238156 23.733257391202063 27.24877961201413 27.98519128440225 48 19.6754092981647 20.152420810207612 36.830489114789216 23.341680776838476 49 21.498949088849198 19.721841307773026 32.51509814179098 26.264111461586797 50 20.093916478101118 20.821970388759738 28.712935648330152 30.371177484808992 51 19.086960963061788 20.625098668020417 34.87972561777789 25.40821475113991 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3212.0 1 2485.0 2 1758.0 3 1194.0 4 630.0 5 742.0 6 854.0 7 800.5 8 747.0 9 702.0 10 657.0 11 651.5 12 646.0 13 666.0 14 686.0 15 677.5 16 669.0 17 707.5 18 746.0 19 741.0 20 736.0 21 821.0 22 906.0 23 1134.0 24 1362.0 25 1588.5 26 2060.5 27 2306.0 28 2766.5 29 3227.0 30 3585.5 31 3944.0 32 4584.5 33 5225.0 34 6008.5 35 6792.0 36 6798.0 37 6804.0 38 7949.0 39 9094.0 40 10491.5 41 11889.0 42 14643.5 43 17398.0 44 28388.0 45 39378.0 46 39147.5 47 38917.0 48 38552.5 49 38188.0 50 34335.0 51 30482.0 52 26496.0 53 22510.0 54 19545.5 55 16581.0 56 14990.0 57 13399.0 58 11528.5 59 9658.0 60 9010.5 61 8363.0 62 7832.5 63 7302.0 64 5852.0 65 4402.0 66 3943.0 67 3484.0 68 2981.0 69 2478.0 70 2279.5 71 2081.0 72 1734.5 73 1388.0 74 1212.0 75 757.0 76 478.0 77 402.5 78 327.0 79 292.0 80 257.0 81 203.0 82 149.0 83 88.0 84 27.0 85 33.5 86 40.0 87 27.0 88 14.0 89 9.0 90 4.0 91 4.5 92 5.0 93 3.0 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 323053.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.937074018732186 #Duplication Level Percentage of deduplicated Percentage of total 1 76.80820682244557 24.53029386534535 2 9.6356740620682 6.154704714812998 3 3.251159115617068 3.114975279664146 4 1.5844600362093837 2.0241206982456865 5 0.9989226492760773 1.595133329445907 6 0.7781520921616099 1.4911140579117985 7 0.5813658432659912 1.299698677784403 8 0.4265346821073348 1.0897815771214685 9 0.41055211271125885 1.180064989098572 >10 5.148383420478569 37.143813592530456 >50 0.3046676257718085 5.925208707811725 >100 0.05693788416063307 3.5264769857400964 >500 0.004994551242160795 1.070321176692421 >1k 0.006992371739025113 4.096889583035491 >5k 0.002996730745296477 5.757402764759501 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT 6084 1.8832823097138858 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC 6058 1.8752340947151085 No Hit AATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGT 5905 1.8278734449146115 No Hit CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT 4069 1.259545647308646 TruSeq Adapter, Index 16 (95% over 23bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2785 0.8620876450613367 No Hit AATGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT 1390 0.4302699557038628 No Hit AATGATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT 1294 0.4005534695545313 No Hit AACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG 1211 0.3748610909045884 No Hit AAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT 1073 0.33214364206492436 No Hit AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTAT 1020 0.31573766533664754 No Hit AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTCCTTTC 804 0.2488755715006516 No Hit AAAAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT 722 0.2234927395814309 No Hit AATGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC 644 0.19934809458509906 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 627 0.19408580016282156 No Hit CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC 558 0.17272707574298954 TruSeq Adapter, Index 19 (95% over 21bp) GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 482 0.14920152420810207 No Hit AAAAAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT 386 0.11948503805877053 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 379 0.11731821094371513 No Hit GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 324 0.10029314075399391 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.461989209200967 0.0 2 0.0 0.0 0.0 1.9027837537493848 0.0 3 0.0 0.0 0.0 2.782825109192609 0.0 4 0.0 0.0 0.0 6.808480342234866 0.0 5 0.0 0.0 0.0 7.3551398686902765 0.0 6 0.0 0.0 0.0 9.531872479128811 0.0 7 0.0 0.0 0.0 11.287002442323706 0.0 8 0.0 0.0 0.0 12.345652261393642 0.0 9 0.0 0.0 0.0 15.519434891488393 0.0 10 0.0 0.0 0.0 17.4058126685095 0.0 11 0.0 0.0 0.0 21.251311704271437 0.0 12 0.0 0.0 0.0 22.330391607569037 0.0 13 0.0 0.0 0.0 22.87055065267928 0.0 14 0.0 0.0 0.0 23.62058238121918 0.0 15 0.0 0.0 0.0 24.025469505003823 0.0 16 0.0 0.0 0.0 24.694709536825226 0.0 17 0.0 0.0 0.0 25.46362361593918 0.0 18 0.0 0.0 0.0 26.925612825140146 0.0 19 0.0 0.0 0.0 27.43048354294806 0.0 20 0.0 0.0 0.0 27.888922251147644 0.0 21 0.0 0.0 0.0 28.384506567033892 0.0 22 0.0 0.0 0.0 28.834277966773254 0.0 23 0.0 0.0 0.0 29.331410016313114 0.0 24 0.0 0.0 0.0 29.65705317703287 0.0 25 0.0 0.0 0.0 29.933478407567797 0.0 26 0.0 0.0 0.0 30.18761627349073 0.0 27 0.0 0.0 0.0 30.487876602291266 0.0 28 0.0 0.0 0.0 30.76646865994125 0.0 29 0.0 0.0 0.0 31.066419442011064 0.0 30 0.0 0.0 0.0 31.49173665002337 3.0954673072220346E-4 31 0.0 0.0 0.0 31.780543749787185 3.0954673072220346E-4 32 0.0 0.0 0.0 32.069350849551 3.0954673072220346E-4 33 0.0 0.0 0.0 32.38385032796476 3.0954673072220346E-4 34 0.0 0.0 0.0 32.65006051638586 3.0954673072220346E-4 35 6.190934614444069E-4 0.0 0.0 32.98313279864295 3.0954673072220346E-4 36 6.190934614444069E-4 0.0 0.0 33.2264365289906 3.0954673072220346E-4 37 6.190934614444069E-4 0.0 0.0 33.49047989029664 3.0954673072220346E-4 38 6.190934614444069E-4 0.0 0.0 33.73625999449007 3.0954673072220346E-4 39 6.190934614444069E-4 0.0 0.0 34.00928021098705 3.0954673072220346E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACGA 35 1.2083001E-7 45.000004 9 TACCCAA 20 7.0257485E-4 45.000004 39 CCGATTG 20 7.0257485E-4 45.000004 43 CGACGGT 20 7.0257485E-4 45.000004 28 TGGACGG 20 7.0257485E-4 45.000004 2 CGGCATC 20 7.0257485E-4 45.000004 33 CGCATCG 20 7.0257485E-4 45.000004 21 TTCGTTA 20 7.0257485E-4 45.000004 38 TTGTAGG 20 7.0257485E-4 45.000004 2 TGACGAC 20 7.0257485E-4 45.000004 18 ACGATTT 35 1.2083001E-7 45.000004 12 TCTACGG 20 7.0257485E-4 45.000004 2 CCTCCGC 70 0.0 45.000004 45 ATTCACG 20 7.0257485E-4 45.000004 1 CATAGTA 20 7.0257485E-4 45.000004 20 TACGATT 35 1.2083001E-7 45.000004 11 CGTAGCG 40 6.7884685E-9 45.000004 1 TCTAAGG 35 1.2083001E-7 45.000004 2 ATGGTCG 20 7.0257485E-4 45.000004 44 TTAAACG 20 7.0257485E-4 45.000004 1 >>END_MODULE