SRR3548656_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548656_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	178090
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCC	10562	5.930709191981582	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGT	9406	5.281599191420069	No Hit
AATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCT	8368	4.698747824133865	No Hit
CTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT	3424	1.9226233926666292	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2518	1.4138918524341624	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTT	2309	1.2965354595990792	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCT	1926	1.081475658374979	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTAT	1622	0.9107754506148578	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTGTCGATC	1593	0.8944915492166882	No Hit
AACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG	1416	0.7951035993037229	No Hit
AAACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCT	790	0.44359593463978886	No Hit
AATGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	698	0.39193666123869947	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	597	0.33522376326576453	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTT	542	0.30434050199337415	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATG	437	0.24538154865517436	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGC	436	0.24482003481385817	No Hit
AATCTGTCTCTTATACCCATCTGACGCCTTGTCGATCGTATGCCGTCTTCT	423	0.2375203548767477	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTA	420	0.23583581335279913	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	415	0.23302824414621817	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCCTTGTCGATCGTATGCCGT	411	0.23078218878095344	No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	342	0.19203773373013644	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	312	0.17519231849065078	No Hit
AATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTCTGCCGTCTTCT	288	0.16171598629906228	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCT	276	0.154977820203268	No Hit
AATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTTGTCGAT	269	0.15104722331405468	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTGTCGCTCGTATGCCGT	240	0.13476332191588522	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	230	0.12914818350272333	No Hit
AATGATCCGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCC	222	0.12465607277219383	No Hit
AATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCTTGTCGATCGT	203	0.11398730978718626	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACCTCTGACGCCTTGTCGATC	185	0.10388006064349486	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTTA	25	3.8774913E-5	45.000004	9
TATCAGA	25	3.8774913E-5	45.000004	45
GTTTATT	25	3.8774913E-5	45.000004	11
AGTGCAG	25	3.8774913E-5	45.000004	1
CGTAGGG	25	3.8774913E-5	45.000004	3
ACAATCG	25	3.8774913E-5	45.000004	1
ACGTGGG	25	3.8774913E-5	45.000004	3
TATTAGG	25	3.8774913E-5	45.000004	2
CGGGATA	25	3.8774913E-5	45.000004	6
GCGATTA	25	3.8774913E-5	45.000004	23
GACGGGA	25	3.8774913E-5	45.000004	4
CACCCGT	25	3.8774913E-5	45.000004	15
AGCAACG	20	7.0172176E-4	45.0	1
GCAAGCG	20	7.0172176E-4	45.0	1
TCACATG	20	7.0172176E-4	45.0	17
CCAGGGT	20	7.0172176E-4	45.0	4
CGCATGG	20	7.0172176E-4	45.0	2
GTGATAG	20	7.0172176E-4	45.0	1
AGGGTGT	20	7.0172176E-4	45.0	6
AGGGTAT	20	7.0172176E-4	45.0	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content