##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548656_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	178090
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.86238980290864	31.0	30.0	33.0	28.0	34.0
2	31.13502161828289	31.0	31.0	34.0	30.0	34.0
3	31.244303442079847	31.0	31.0	34.0	30.0	34.0
4	35.217816834184966	35.0	35.0	37.0	33.0	37.0
5	34.34538716379359	35.0	35.0	37.0	30.0	37.0
6	34.58371609860183	35.0	35.0	37.0	32.0	37.0
7	33.99664214722893	35.0	35.0	37.0	30.0	37.0
8	34.623752035487676	35.0	35.0	37.0	32.0	37.0
9	36.23333146162053	37.0	35.0	39.0	32.0	39.0
10	35.83047335616823	37.0	35.0	39.0	31.0	39.0
11	35.995243977764055	37.0	35.0	39.0	31.0	39.0
12	35.31683418496266	37.0	34.0	39.0	30.0	39.0
13	35.048436183951935	37.0	34.0	39.0	30.0	39.0
14	35.8911673872761	38.0	34.0	40.0	29.0	41.0
15	36.21796282778371	38.0	35.0	40.0	31.0	41.0
16	35.78410354315234	37.0	34.0	40.0	30.0	41.0
17	35.757499017350774	37.0	34.0	40.0	30.0	41.0
18	35.459700151608736	37.0	34.0	40.0	28.0	41.0
19	35.20464371946768	37.0	33.0	40.0	27.0	41.0
20	35.444174293896346	37.0	34.0	40.0	29.0	41.0
21	35.830512662137124	37.0	34.0	40.0	30.0	41.0
22	35.59696221011848	37.0	34.0	40.0	29.0	41.0
23	35.58162726711213	37.0	34.0	40.0	29.0	41.0
24	34.99049918580493	37.0	33.0	39.0	27.0	41.0
25	35.3419787747768	37.0	34.0	40.0	28.0	41.0
26	35.11879386826885	37.0	34.0	40.0	27.0	41.0
27	35.071357178954464	37.0	33.0	40.0	27.0	41.0
28	35.15171542478522	37.0	34.0	40.0	27.0	41.0
29	34.69866359705767	37.0	33.0	40.0	25.0	41.0
30	34.955415800999496	37.0	34.0	40.0	27.0	41.0
31	34.63430849570442	37.0	33.0	40.0	25.0	40.0
32	34.43558313212421	37.0	33.0	40.0	25.0	41.0
33	34.62815991914201	37.0	33.0	40.0	25.0	41.0
34	34.47677578752316	37.0	33.0	40.0	25.0	41.0
35	34.43120332416194	37.0	33.0	40.0	25.0	41.0
36	34.47906114885732	37.0	33.0	40.0	25.0	41.0
37	34.338143635240606	37.0	33.0	40.0	24.0	40.0
38	34.10389129092032	36.0	33.0	39.0	24.0	40.0
39	33.9268347464765	36.0	33.0	39.0	23.0	40.0
40	33.32285361334157	36.0	32.0	39.0	21.0	40.0
41	33.71187040260543	36.0	33.0	39.0	23.0	40.0
42	33.63231512156775	36.0	33.0	39.0	23.0	40.0
43	33.877932506036274	36.0	33.0	39.0	23.0	40.0
44	33.863445448930314	36.0	33.0	39.0	23.0	40.0
45	33.313145039025216	35.0	32.0	39.0	21.0	40.0
46	33.27378853388736	35.0	32.0	39.0	22.0	40.0
47	33.17638272783424	35.0	32.0	39.0	22.0	40.0
48	33.33827278342411	35.0	32.0	39.0	22.0	40.0
49	33.19605817283396	36.0	32.0	39.0	21.0	40.0
50	33.097866247403	35.0	32.0	39.0	21.0	40.0
51	31.504323656578133	35.0	28.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	4.0
11	2.0
12	3.0
13	9.0
14	7.0
15	22.0
16	33.0
17	89.0
18	190.0
19	394.0
20	721.0
21	1092.0
22	1466.0
23	1662.0
24	1907.0
25	2120.0
26	2329.0
27	2820.0
28	3653.0
29	4538.0
30	6143.0
31	8281.0
32	10866.0
33	14046.0
34	20177.0
35	20958.0
36	19932.0
37	23053.0
38	21807.0
39	9761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.160817564152955	53.59312707058229	13.290471110112865	18.955584255151887
2	14.280420012353304	55.1715424785221	14.879555281037677	15.668482228086923
3	14.567915099107193	25.775731371778317	42.380818687180636	17.275534841933855
4	35.3034982312314	25.83356729743388	15.674097366500083	23.188837104834633
5	12.193273064181032	48.26436071649166	18.907855578640014	20.634510640687292
6	18.70683362344882	27.88309281823797	36.81790106126116	16.59217249705205
7	48.835420293110225	28.063338761300464	14.106350721545285	8.994890224044022
8	51.231961367847724	8.824190016283902	9.45477006008198	30.4890785557864
9	59.89780448088046	10.10163400527823	17.903307316525353	12.097254197315964
10	38.20708630467741	23.437587736537704	18.475489920826547	19.879836037958334
11	27.868493458363748	19.846145207479367	26.774664495479815	25.51069683867707
12	25.460160592958616	20.522207872424055	40.43629625470268	13.581335279914649
13	22.521197147509685	35.98349149306531	19.69341344264136	21.80189791678365
14	9.961255544949182	29.72317367623112	43.283732944017075	17.031837834802626
15	8.319389072940648	29.87253635802122	27.834802627884773	33.97327194115335
16	9.514290527261497	28.140266157560784	39.38121174686956	22.964231568308158
17	10.040428996574766	27.36144645965523	29.56538828682127	33.03273625694874
18	12.730080296479308	28.641698017856136	26.61407153686338	32.01415014880117
19	15.593239373350553	31.108428322758158	36.6421472289292	16.6561850749621
20	18.091975967207592	38.82025941939469	23.503846369813015	19.583918243584705
21	12.598124543770004	27.48666404626874	30.20495255208041	29.710258857880845
22	13.12931663765512	37.577629288561965	26.799371104497727	22.493682969285192
23	20.038744455050818	27.268235161996746	19.63164692009658	33.06137346285586
24	10.850693469594026	43.187714077152	23.386489976977934	22.57510247627604
25	13.84636981301589	24.412937278903925	29.117861755292267	32.62283115278792
26	20.629457016115445	34.237183446571954	20.564882924364085	24.56847661294851
27	10.528384524678533	29.713627940928745	33.343814925037904	26.414172609354818
28	21.42736818462575	22.350496939749565	24.816665730810264	31.40546914481442
29	13.239373350553091	31.68847212083778	37.16154753214666	17.910606996462462
30	20.750744005839746	22.251670503677914	27.016115447245774	29.981470043236563
31	32.62620023583581	29.270593520130273	19.37840417766298	18.724802066370934
32	18.42832275815599	21.895670728283452	28.32219664214723	31.35380987141333
33	26.933572912572295	21.404907630973103	20.582851367286203	31.0786680891684
34	23.18209893873884	21.160649110000563	28.299174574653264	27.35807737660733
35	15.576393958111067	26.071649166151943	28.79442978269414	29.557527093042847
36	27.16098601830535	18.290190353192205	37.1593014767814	17.38952215172104
37	19.17682070863047	24.599359874220898	33.26857207030153	22.9552473468471
38	28.78993767196361	24.006401257791005	23.526306923465665	23.67735414677972
39	23.907574821719354	19.84109158290752	35.56404065360211	20.687292941771016
40	20.969172890111743	29.712504913246114	19.889381773260713	29.428940423381437
41	25.49216688191364	17.325509573810994	34.33600988264361	22.846313661631758
42	24.74647650064574	26.993654893593128	20.818687180639003	27.44118142512213
43	21.72665506204728	19.085855466337247	32.73962603178168	26.447863439833792
44	19.064517940367228	18.15542703127632	29.692851928800046	33.087203099556405
45	32.62676174967713	27.22219102700882	21.43972148913471	18.711325734179347
46	15.409062833398842	19.16895951485204	45.13167499578864	20.290302655960467
47	23.466786456286147	24.671795159750687	21.14043461171318	30.720983772249987
48	20.230220674939638	16.17384468527149	41.327418720871464	22.2685159189174
49	23.99348643944073	17.254758829805155	31.275197933629062	27.476556797125053
50	20.51266213712168	18.622045033410075	25.113145039025213	35.75214779044303
51	15.353472963108542	17.59559773148408	40.26840361614914	26.78252568925824
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2835.0
1	2213.0
2	1591.0
3	1077.5
4	564.0
5	560.0
6	556.0
7	542.0
8	528.0
9	491.0
10	454.0
11	495.5
12	537.0
13	551.0
14	565.0
15	583.0
16	601.0
17	613.0
18	625.0
19	626.5
20	628.0
21	594.5
22	561.0
23	630.5
24	700.0
25	953.5
26	1129.0
27	1051.0
28	1005.0
29	959.0
30	1029.0
31	1099.0
32	1253.0
33	1407.0
34	1566.0
35	1725.0
36	1924.0
37	2123.0
38	2592.5
39	3062.0
40	3682.0
41	4302.0
42	6919.5
43	9537.0
44	23367.0
45	37197.0
46	34609.5
47	32022.0
48	26830.5
49	21639.0
50	17706.5
51	13774.0
52	11712.5
53	9651.0
54	8131.5
55	6612.0
56	5815.0
57	5018.0
58	4376.5
59	3735.0
60	3514.0
61	3293.0
62	2965.5
63	2638.0
64	2073.5
65	1509.0
66	1419.0
67	1329.0
68	1058.5
69	788.0
70	667.0
71	546.0
72	448.0
73	350.0
74	318.0
75	249.0
76	212.0
77	162.0
78	112.0
79	89.0
80	66.0
81	64.0
82	62.0
83	43.5
84	25.0
85	15.0
86	5.0
87	4.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	178090.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.52237632657645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.90284757118927	27.455780784996346
2	7.876046901172529	5.280476163737436
3	2.4974874371859297	2.5116514122073106
4	1.2981574539363483	1.7406929080801843
5	0.8073701842546064	1.3532483575720142
6	0.5510887772194305	1.108428322758156
7	0.4673366834170854	1.096636532090516
8	0.39195979899497485	1.0511539109439048
9	0.3869346733668342	1.1673872760963557
>10	3.6398659966499167	24.710539614801505
>50	0.09045226130653267	2.1534055814475828
>100	0.06700167504187605	4.669549104385423
>500	0.006700167504187606	1.475096861137627
>1k	0.011725293132328308	8.314896962210119
>5k	0.003350083752093803	9.980347015553933
>10k+	0.0016750418760469014	5.930709191981582
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCC	10562	5.930709191981582	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGT	9406	5.281599191420069	No Hit
AATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCT	8368	4.698747824133865	No Hit
CTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT	3424	1.9226233926666292	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2518	1.4138918524341624	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTT	2309	1.2965354595990792	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCT	1926	1.081475658374979	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTAT	1622	0.9107754506148578	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTGTCGATC	1593	0.8944915492166882	No Hit
AACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG	1416	0.7951035993037229	No Hit
AAACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCT	790	0.44359593463978886	No Hit
AATGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	698	0.39193666123869947	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	597	0.33522376326576453	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTT	542	0.30434050199337415	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATG	437	0.24538154865517436	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGC	436	0.24482003481385817	No Hit
AATCTGTCTCTTATACCCATCTGACGCCTTGTCGATCGTATGCCGTCTTCT	423	0.2375203548767477	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTA	420	0.23583581335279913	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	415	0.23302824414621817	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCCTTGTCGATCGTATGCCGT	411	0.23078218878095344	No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	342	0.19203773373013644	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	312	0.17519231849065078	No Hit
AATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTCTGCCGTCTTCT	288	0.16171598629906228	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCT	276	0.154977820203268	No Hit
AATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTTGTCGAT	269	0.15104722331405468	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTGTCGCTCGTATGCCGT	240	0.13476332191588522	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	230	0.12914818350272333	No Hit
AATGATCCGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCC	222	0.12465607277219383	No Hit
AATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCTTGTCGATCGT	203	0.11398730978718626	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACCTCTGACGCCTTGTCGATC	185	0.10388006064349486	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.615138413161884E-4	0.0	0.0	2.354427536638778	0.0
2	5.615138413161884E-4	0.0	0.0	2.8659666460778257	0.0
3	5.615138413161884E-4	0.0	0.0	4.300634510640688	0.0
4	5.615138413161884E-4	0.0	0.0	11.98158234600483	0.0
5	5.615138413161884E-4	0.0	0.0	12.85361334156887	0.0
6	5.615138413161884E-4	0.0	0.0	16.113201190409345	0.0
7	5.615138413161884E-4	0.0	0.0	18.683811555954854	0.0
8	5.615138413161884E-4	0.0	0.0	19.692851928800046	0.0
9	5.615138413161884E-4	0.0	0.0	26.91055084507833	0.0
10	5.615138413161884E-4	0.0	0.0	28.647874670110618	0.0
11	5.615138413161884E-4	0.0	0.0	37.253074288281205	0.0
12	5.615138413161884E-4	0.0	0.0	38.54062552641923	0.0
13	5.615138413161884E-4	0.0	0.0	39.369981470043236	0.0
14	5.615138413161884E-4	0.0	0.0	40.86136223257903	0.0
15	5.615138413161884E-4	0.0	0.0	41.468358695041836	0.0
16	5.615138413161884E-4	0.0	0.0	42.20281879948341	0.0
17	5.615138413161884E-4	0.0	0.0	42.90414958728733	0.0
18	5.615138413161884E-4	0.0	0.0	45.391094390476724	0.0
19	5.615138413161884E-4	0.0	0.0	45.98966814531978	0.0
20	5.615138413161884E-4	0.0	0.0	46.41417260935482	0.0
21	5.615138413161884E-4	0.0	0.0	46.889774832949634	0.0
22	5.615138413161884E-4	0.0	0.0	47.23342130383514	0.0
23	5.615138413161884E-4	0.0	0.0	47.59391318996013	0.0
24	5.615138413161884E-4	0.0	0.0	47.83480262788478	0.0
25	5.615138413161884E-4	0.0	0.0	48.047054859902296	0.0
26	5.615138413161884E-4	0.0	0.0	48.255938008871915	0.0
27	5.615138413161884E-4	0.0	0.0	48.48110505923971	0.0
28	5.615138413161884E-4	0.0	0.0	48.707956651131454	0.0
29	5.615138413161884E-4	0.0	0.0	48.92694704924477	0.0
30	5.615138413161884E-4	0.0	0.0	49.27620865854343	0.0
31	5.615138413161884E-4	0.0	0.0	49.496883598180695	0.0
32	5.615138413161884E-4	0.0	0.0	49.672075916671346	0.0
33	5.615138413161884E-4	0.0	0.0	49.87141333033859	0.0
34	5.615138413161884E-4	0.0	0.0	50.02751417822449	0.0
35	5.615138413161884E-4	0.0	0.0	50.226290078050425	0.0
36	5.615138413161884E-4	0.0	0.0	50.38969060587343	0.0
37	5.615138413161884E-4	0.0	0.0	50.55477567522039	0.0
38	5.615138413161884E-4	0.0	0.0	50.74232129822	0.0
39	5.615138413161884E-4	0.0	0.0	50.90740636756696	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTTA	25	3.8774913E-5	45.000004	9
TATCAGA	25	3.8774913E-5	45.000004	45
GTTTATT	25	3.8774913E-5	45.000004	11
AGTGCAG	25	3.8774913E-5	45.000004	1
CGTAGGG	25	3.8774913E-5	45.000004	3
ACAATCG	25	3.8774913E-5	45.000004	1
ACGTGGG	25	3.8774913E-5	45.000004	3
TATTAGG	25	3.8774913E-5	45.000004	2
CGGGATA	25	3.8774913E-5	45.000004	6
GCGATTA	25	3.8774913E-5	45.000004	23
GACGGGA	25	3.8774913E-5	45.000004	4
CACCCGT	25	3.8774913E-5	45.000004	15
AGCAACG	20	7.0172176E-4	45.0	1
GCAAGCG	20	7.0172176E-4	45.0	1
TCACATG	20	7.0172176E-4	45.0	17
CCAGGGT	20	7.0172176E-4	45.0	4
CGCATGG	20	7.0172176E-4	45.0	2
GTGATAG	20	7.0172176E-4	45.0	1
AGGGTGT	20	7.0172176E-4	45.0	6
AGGGTAT	20	7.0172176E-4	45.0	6
>>END_MODULE