##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548654_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410857
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15733698099339	31.0	31.0	33.0	28.0	34.0
2	31.414762313895103	31.0	31.0	34.0	30.0	34.0
3	31.572440046050087	31.0	31.0	34.0	30.0	34.0
4	35.5145902345585	37.0	35.0	37.0	33.0	37.0
5	34.90058584860426	35.0	35.0	37.0	32.0	37.0
6	34.985603263422554	35.0	35.0	37.0	32.0	37.0
7	34.943578909450245	36.0	35.0	37.0	32.0	37.0
8	35.3564257150298	37.0	35.0	37.0	33.0	37.0
9	36.821142149214936	39.0	37.0	39.0	33.0	39.0
10	36.234604740822235	38.0	35.0	39.0	32.0	39.0
11	36.49141185375934	38.0	35.0	39.0	32.0	39.0
12	36.243016426639926	37.0	35.0	39.0	32.0	39.0
13	35.93477049192298	37.0	35.0	39.0	31.0	39.0
14	36.94449893758656	39.0	35.0	40.0	31.0	41.0
15	37.01787239842573	39.0	35.0	40.0	32.0	41.0
16	36.833095213176364	38.0	35.0	40.0	32.0	41.0
17	36.77212266068243	38.0	35.0	40.0	32.0	41.0
18	36.65742581968909	38.0	35.0	40.0	31.0	41.0
19	36.74339490382298	38.0	35.0	40.0	31.0	41.0
20	36.6664240842921	38.0	35.0	40.0	31.0	41.0
21	36.790915087244464	38.0	35.0	40.0	31.0	41.0
22	36.737823622330886	39.0	35.0	40.0	31.0	41.0
23	36.684892797250626	38.0	35.0	40.0	31.0	41.0
24	36.26784258269909	38.0	35.0	40.0	30.0	41.0
25	36.45649946331692	38.0	35.0	40.0	31.0	41.0
26	36.41352100609215	38.0	35.0	40.0	31.0	41.0
27	36.32225324139542	38.0	35.0	40.0	30.0	41.0
28	36.29273932292744	38.0	35.0	40.0	30.0	41.0
29	35.79206633938329	38.0	34.0	40.0	29.0	41.0
30	35.90842069138411	38.0	35.0	40.0	29.0	41.0
31	35.729484954619245	38.0	34.0	40.0	29.0	41.0
32	35.79763275300165	38.0	35.0	40.0	29.0	41.0
33	35.75188690955734	38.0	35.0	40.0	29.0	41.0
34	35.628354877731184	38.0	34.0	40.0	28.0	41.0
35	35.661996753128214	38.0	34.0	40.0	29.0	41.0
36	35.609725524939336	38.0	34.0	40.0	29.0	41.0
37	35.502323192741024	38.0	34.0	40.0	28.0	41.0
38	35.36513190720859	38.0	34.0	40.0	28.0	41.0
39	35.241190973988516	38.0	34.0	40.0	27.0	41.0
40	35.05181608199447	38.0	34.0	40.0	26.0	41.0
41	35.04936754150471	38.0	34.0	40.0	26.0	41.0
42	35.0692625414682	38.0	34.0	40.0	27.0	41.0
43	35.03492212618989	37.0	34.0	40.0	26.0	41.0
44	35.00642315939609	37.0	34.0	40.0	26.0	41.0
45	34.81886641824284	37.0	34.0	40.0	26.0	41.0
46	34.72052076513239	37.0	33.0	40.0	26.0	41.0
47	34.678046132839405	37.0	33.0	40.0	26.0	41.0
48	34.665693903231535	37.0	33.0	40.0	26.0	41.0
49	34.53299566515844	37.0	33.0	40.0	25.0	41.0
50	34.37721396982405	37.0	33.0	40.0	24.0	41.0
51	33.17645798903268	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	8.0
11	7.0
12	15.0
13	15.0
14	18.0
15	45.0
16	77.0
17	156.0
18	279.0
19	646.0
20	1117.0
21	1702.0
22	2239.0
23	2771.0
24	3170.0
25	3546.0
26	4043.0
27	4637.0
28	5718.0
29	7119.0
30	9280.0
31	12465.0
32	17135.0
33	24266.0
34	38440.0
35	41234.0
36	45998.0
37	63276.0
38	76140.0
39	45289.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.480361780376143	35.96166062644667	18.31513154211806	25.242846051059125
2	23.125077581737685	36.061938825430744	17.97924825425878	22.83373533857279
3	20.27006963493381	31.262945501719575	23.702407406956677	24.764577456389937
4	22.796496104484042	31.17702753025992	18.5580384415989	27.468437923657135
5	17.124936413399308	36.000360222656546	19.02754486354133	27.84715850040282
6	20.020104318534184	35.95460220952789	22.652407041866148	21.372886430071777
7	75.03851705094473	10.702993985741998	8.172673217202092	6.0858157461111775
8	76.0235313016451	7.355113823057657	6.9880761432809955	9.633278732016247
9	72.0440445215732	10.07941936002064	11.461652107667632	6.414884010738529
10	34.12233453488683	34.62688964773632	14.568815914052822	16.681959903324028
11	27.914335157974673	28.810510712973127	25.71356944143583	17.56158468761637
12	28.167464592303403	24.106684320822087	31.815449170879408	15.910401915995104
13	24.772122660682427	31.61927385927464	24.260265737227307	19.348337742815627
14	16.000457580131286	34.646604536371534	30.188362374256737	19.164575509240443
15	15.071910664781177	29.842256551549568	32.930679044046954	22.155153739622303
16	16.726987735392118	30.6223333179184	32.043265661775266	20.607413284914216
17	17.29969308056088	26.891594885811855	28.09468987993389	27.714022153693378
18	21.303275835631375	26.629216491382646	27.68773563551309	24.37977203747289
19	22.473756075714906	30.545907700246072	27.562631280469844	19.417704943569174
20	23.205154104712832	30.27306337728212	24.73804754452279	21.783734973482257
21	20.125980572315918	30.108529244968445	27.697227989300416	22.06826219341523
22	18.355534894135918	31.19674241889514	26.024869966922797	24.422852720046148
23	19.98773295818253	30.984989911331677	22.757553114587314	26.269724015898475
24	16.7099501773123	32.015031994100134	27.08996074059831	24.18505708798925
25	18.046668305517493	29.08627576017933	27.616664678951558	25.250391255351616
26	20.576015499309978	31.409955288579727	24.78623949451999	23.227789717590305
27	17.398024130050118	29.351088091477084	27.600357301932306	25.6505304765405
28	19.573963690529794	28.14799309735504	30.645455718169583	21.632587493945582
29	20.063915182168003	29.822054875540637	29.708146630092706	20.405883312198647
30	20.976398114185717	27.08314571736638	29.196046312950735	22.744409855497167
31	22.862455793621624	30.68172137751091	23.305188910010052	23.150633918857412
32	25.481128470489733	26.720732517639956	25.652477626035335	22.145661385834973
33	22.04903409215372	25.266942026057727	27.21822921357066	25.465794668217896
34	20.36109887381741	27.751017994095267	28.397958413754665	23.489924718332656
35	21.474868384863832	25.73669184168701	30.291804691169922	22.496635082279237
36	21.66349849217611	26.532832591388246	31.057521230014334	20.74614768642131
37	21.726537457071437	28.712423057170746	26.06186580732469	23.49917367843313
38	22.630988397422946	27.002339013330673	25.482345438924003	24.884327150322374
39	24.00105146072721	25.003346663194247	26.197679484589532	24.79792239148901
40	24.87337443441392	26.43717887245441	24.321357552627802	24.368089140503873
41	20.19242704882721	25.180537267224363	28.774488447318653	25.852547236629775
42	26.940517016869613	24.283631531165344	26.353694837863294	22.422156614101745
43	21.592184141927724	25.14670554475158	28.417186515016173	24.84392379830452
44	21.818540270702456	23.8158288650309	27.37838225952095	26.98724860474569
45	24.78234519553032	26.14486305454209	25.753972793453684	23.31881895647391
46	20.592322876329234	25.81749854572272	30.726992603265856	22.86318597468219
47	21.322990724266592	25.540759923769095	25.92556534268614	27.210684009278168
48	21.278449679572212	24.18067600162587	32.470421582205	22.07045273659692
49	24.068471511985923	21.689298222982693	29.65313965686358	24.589090608167805
50	19.367322450390283	24.60053011144997	28.141664861496825	27.89048257666293
51	20.319721947052138	23.422504667073945	31.159746578493248	25.09802680738067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4490.0
1	3572.0
2	2654.0
3	1781.5
4	909.0
5	1116.0
6	1323.0
7	1190.5
8	1058.0
9	1033.5
10	1009.0
11	1141.0
12	1273.0
13	1171.0
14	1069.0
15	1098.0
16	1127.0
17	1153.0
18	1179.0
19	1180.0
20	1181.0
21	1162.5
22	1144.0
23	1433.5
24	1723.0
25	2115.0
26	2958.0
27	3409.0
28	3904.5
29	4400.0
30	5028.0
31	5656.0
32	6523.5
33	7391.0
34	8474.0
35	9557.0
36	10405.5
37	11254.0
38	12090.5
39	12927.0
40	13741.5
41	14556.0
42	18749.5
43	22943.0
44	31934.0
45	40925.0
46	42836.0
47	44747.0
48	45185.5
49	45624.0
50	41901.5
51	38179.0
52	34017.5
53	29856.0
54	26718.0
55	23580.0
56	20216.5
57	16853.0
58	15491.0
59	14129.0
60	13269.5
61	12410.0
62	11125.0
63	9840.0
64	8016.0
65	6192.0
66	5097.5
67	4003.0
68	3474.0
69	2945.0
70	2554.5
71	2164.0
72	1813.5
73	1463.0
74	1380.5
75	1223.0
76	1148.0
77	773.0
78	398.0
79	295.0
80	192.0
81	140.0
82	88.0
83	70.5
84	53.0
85	35.0
86	17.0
87	11.5
88	6.0
89	5.0
90	4.0
91	4.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	410857.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.797864621449946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.29359652137731	26.799542405773845
2	8.977279989637257	6.068257875172232
3	2.983766151120933	3.025347733129501
4	1.4219008312227936	1.9222884719518047
5	0.8748629710390884	1.4784250128749297
6	0.6255258543592765	1.2684862885710986
7	0.4766598308255128	1.127705910290674
8	0.3485246265771305	0.9423510517036202
9	0.30149263328097686	0.9170826483595419
>10	3.953182023178328	31.286201530696655
>50	0.6701890486017037	14.710172791690157
>100	0.06072910472766247	3.4191463385621805
>500	0.007952620857193895	2.015617676466764
>1k	0.004337793194833034	5.019374264756972
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCT	4007	0.9752785032261834	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3974	0.9672465115599832	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCC	3948	0.9609182757017649	No Hit
CTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGCT	3895	0.9480184102984737	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGT	3549	0.8638041946467994	No Hit
AAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCT	1169	0.28452721993296937	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	944	0.22976364039069558	No Hit
AATGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT	902	0.21954110554280445	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCT	869	0.2115091138766043	No Hit
AACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTG	868	0.2112657201897497	No Hit
AATGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTC	814	0.198122461099604	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT	777	0.18911689468598564	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	773	0.18814331993856742	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	655	0.15942286488973048	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTAT	621	0.1511474795366758	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	516	0.125591142416948	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTCGCAGTC	510	0.1241307802958207	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.301810605636511E-4	0.0	0.0	1.095514984532331	0.0
2	7.301810605636511E-4	0.0	0.0	1.5122049764273213	0.0
3	7.301810605636511E-4	0.0	0.0	2.1338324526538432	0.0
4	7.301810605636511E-4	0.0	0.0	4.923854285067554	0.0
5	7.301810605636511E-4	0.0	0.0	5.498750173418002	0.0
6	7.301810605636511E-4	0.0	0.0	7.312033140484402	0.0
7	7.301810605636511E-4	0.0	0.0	8.756818065653013	0.0
8	7.301810605636511E-4	0.0	0.0	9.737694623676852	0.0
9	7.301810605636511E-4	0.0	0.0	11.764190460427837	0.0
10	7.301810605636511E-4	0.0	0.0	13.660957462085348	0.0
11	7.301810605636511E-4	0.0	0.0	16.40278734450186	0.0
12	7.301810605636511E-4	0.0	0.0	17.44962359166328	0.0
13	7.301810605636511E-4	0.0	0.0	18.00480459137851	0.0
14	7.301810605636511E-4	0.0	0.0	18.612802021141174	0.0
15	7.301810605636511E-4	0.0	0.0	18.989818842078876	0.0
16	7.301810605636511E-4	0.0	0.0	19.654283607191797	0.0
17	7.301810605636511E-4	0.0	0.0	20.369861046544173	0.0
18	7.301810605636511E-4	0.0	0.0	21.682969987124473	0.0
19	7.301810605636511E-4	0.0	0.0	22.1123164507359	0.0
20	7.301810605636511E-4	0.0	0.0	22.606892422424348	0.0
21	7.301810605636511E-4	0.0	0.0	23.093679796133447	0.0
22	7.301810605636511E-4	0.0	0.0	23.571704997115784	0.0
23	7.301810605636511E-4	0.0	0.0	24.014438113504212	0.0
24	7.301810605636511E-4	0.0	0.0	24.361030723585092	0.0
25	7.301810605636511E-4	0.0	0.0	24.65042581725515	0.0
26	7.301810605636511E-4	0.0	0.0	24.9125608179975	0.0
27	7.301810605636511E-4	0.0	0.0	25.232136728837528	0.0
28	7.301810605636511E-4	0.0	0.0	25.578485945231552	0.0
29	7.301810605636511E-4	0.0	0.0	25.885161990668287	0.0
30	7.301810605636511E-4	0.0	0.0	26.37487008861964	0.0
31	7.301810605636511E-4	0.0	0.0	26.67448771713759	0.0
32	7.301810605636511E-4	0.0	0.0	26.993576840603907	0.0
33	7.301810605636511E-4	0.0	0.0	27.31193578300966	0.0
34	7.301810605636511E-4	0.0	0.0	27.63662296127363	0.0
35	7.301810605636511E-4	0.0	0.0	27.947193305700036	0.0
36	7.301810605636511E-4	0.0	0.0	28.223201746593098	0.0
37	7.301810605636511E-4	0.0	0.0	28.48387638521432	0.0
38	7.301810605636511E-4	0.0	0.0	28.764022518783907	0.0
39	7.301810605636511E-4	0.0	0.0	29.09138702760328	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGGG	115	0.0	45.000004	3
AATCCCG	25	3.886415E-5	45.0	1
GTACCGG	20	7.0279907E-4	45.0	2
GTCGAAA	25	3.886415E-5	45.0	34
CTATACG	20	7.0279907E-4	45.0	1
GCAAACG	35	1.2093733E-7	45.0	1
CAACCAG	25	3.886415E-5	45.0	1
TGAACGG	25	3.886415E-5	45.0	2
GCCGGAA	35	1.2093733E-7	45.0	27
ATTAACG	20	7.0279907E-4	45.0	1
TACGGGT	20	7.0279907E-4	45.0	4
ATCACGG	80	0.0	45.0	2
AGCGGTA	25	3.886415E-5	45.0	40
CCCAACG	20	7.0279907E-4	45.0	1
CTACGGA	25	3.886415E-5	45.0	29
GCGGCTA	25	3.886415E-5	45.0	23
TTACGTA	20	7.0279907E-4	45.0	12
CGGCTAG	20	7.0279907E-4	45.0	1
TTACGAC	20	7.0279907E-4	45.0	32
CGAGAGT	35	1.2093733E-7	45.0	12
>>END_MODULE