FastQCFastQC Report
Sat 18 Jun 2016
SRR3548653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423287
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCC42711.009008072537073No Hit
AATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCT41880.9893996272032922No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40100.9473477805838592No Hit
CTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGCT39410.9310467838605958No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGT36880.8712764625419632No Hit
AAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCT13050.3083014597660689No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9460.22348902753923464No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCT8870.2095504941091978No Hit
AATGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT8850.20907800145055247No Hit
AACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTG8790.20766052347461653No Hit
AATGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTC8770.2071880308159712No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT8150.1925407583979664No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7850.18545336851828664No Hit
CCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC6700.15828504064618096No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTAT6330.1495439264612426No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.13300668340865654No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTCGCAGTC5050.11930439630794237No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCT4490.10607460186587352No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4330.10229466059671098No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCCG302.16224E-645.0000041
TAGACGG253.8866146E-545.0000042
TTGTCGC302.16224E-645.00000438
GGTTTCG302.16224E-645.0000048
ACGGGTA302.16224E-645.0000045
CCTCGAC253.8866146E-545.00000430
TAGCACG302.16224E-645.0000041
CCCGTTC253.8866146E-545.00000416
ACGATAA253.8866146E-545.00000434
ATATCCG253.8866146E-545.0000041
CCGTAGT253.8866146E-545.00000427
TACGATA253.8866146E-545.00000433
TCGCTCG302.16224E-645.00000421
GCGGGTC302.16224E-645.0000045
TGCCTAG302.16224E-645.0000041
TCCCGCG302.16224E-645.00000415
CTCGACT253.8866146E-545.00000431
TACTACG302.16224E-645.0000041
TTTGCGG351.2095006E-745.0000042
CGAGACG253.8866146E-545.0000041
Produced by FastQC (version 0.11.5)