##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548653_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	423287
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229662144124436	31.0	31.0	33.0	30.0	34.0
2	31.48690604719729	31.0	31.0	34.0	30.0	34.0
3	31.653679418456036	31.0	31.0	34.0	30.0	34.0
4	35.56368846669045	37.0	35.0	37.0	33.0	37.0
5	34.92819292820238	35.0	35.0	37.0	32.0	37.0
6	35.00221835303234	35.0	35.0	37.0	32.0	37.0
7	34.97931190894121	36.0	35.0	37.0	32.0	37.0
8	35.342604426783716	37.0	35.0	37.0	33.0	37.0
9	36.88435505933327	39.0	37.0	39.0	33.0	39.0
10	36.27610817246927	38.0	35.0	39.0	32.0	39.0
11	36.46590374852051	38.0	35.0	39.0	32.0	39.0
12	36.18585262481484	37.0	35.0	39.0	32.0	39.0
13	35.971000763075644	37.0	35.0	39.0	31.0	39.0
14	36.95563766427979	39.0	35.0	40.0	32.0	41.0
15	37.00893483617498	38.0	35.0	40.0	32.0	41.0
16	36.84218508954917	38.0	35.0	40.0	32.0	41.0
17	36.79366718089618	38.0	35.0	40.0	32.0	41.0
18	36.637345347246665	38.0	35.0	40.0	31.0	41.0
19	36.7703236810958	38.0	35.0	40.0	31.0	41.0
20	36.645845490175695	38.0	35.0	40.0	31.0	41.0
21	36.777616605282	38.0	35.0	40.0	31.0	41.0
22	36.731461159922226	38.0	35.0	40.0	31.0	41.0
23	36.6334283831065	38.0	35.0	40.0	31.0	41.0
24	36.22170773021614	38.0	35.0	40.0	30.0	41.0
25	36.40293465190285	38.0	35.0	40.0	31.0	41.0
26	36.29855629868151	38.0	35.0	40.0	30.0	41.0
27	36.16750573487965	38.0	35.0	40.0	30.0	41.0
28	36.102301747986594	38.0	35.0	40.0	30.0	41.0
29	35.73464103551491	38.0	34.0	40.0	29.0	41.0
30	35.83195326102621	38.0	34.0	40.0	29.0	41.0
31	35.664622348430264	38.0	34.0	40.0	29.0	41.0
32	35.71642880598743	38.0	34.0	40.0	29.0	41.0
33	35.64598723797329	38.0	34.0	40.0	29.0	41.0
34	35.516611660646326	38.0	34.0	40.0	28.0	41.0
35	35.54685355326291	38.0	34.0	40.0	28.0	41.0
36	35.470529451648645	38.0	34.0	40.0	28.0	41.0
37	35.35958581293543	38.0	34.0	40.0	27.0	41.0
38	35.26531644014581	38.0	34.0	40.0	27.0	41.0
39	35.16137514263372	38.0	34.0	40.0	27.0	41.0
40	34.935183457087035	37.0	34.0	40.0	26.0	41.0
41	34.9439505583682	37.0	34.0	40.0	26.0	41.0
42	34.97542801928715	37.0	34.0	40.0	26.0	41.0
43	34.96375981308191	37.0	34.0	40.0	26.0	41.0
44	34.87443507596501	37.0	34.0	40.0	26.0	41.0
45	34.7027666807627	37.0	33.0	40.0	25.0	41.0
46	34.569516663634836	37.0	33.0	40.0	25.0	41.0
47	34.50873520802671	37.0	33.0	40.0	25.0	41.0
48	34.49671026986418	37.0	33.0	40.0	25.0	41.0
49	34.34767427301098	37.0	33.0	40.0	24.0	41.0
50	34.21284140547666	37.0	33.0	40.0	24.0	41.0
51	32.93825938429482	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	2.0
10	5.0
11	9.0
12	12.0
13	14.0
14	28.0
15	35.0
16	77.0
17	169.0
18	334.0
19	694.0
20	1168.0
21	1764.0
22	2363.0
23	2847.0
24	3465.0
25	3739.0
26	4192.0
27	5080.0
28	5974.0
29	7677.0
30	10078.0
31	13265.0
32	17994.0
33	25052.0
34	39512.0
35	42987.0
36	47996.0
37	63720.0
38	76989.0
39	46040.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.424440155261088	36.14946832763586	18.2694011391798	25.15669037792325
2	23.13702050854385	36.153012022575695	18.029138622258657	22.680828846621797
3	20.222449543690217	31.287991362834198	23.557775221067505	24.931783872408083
4	22.686735004854864	31.351541625421998	18.61172207036833	27.35000129935481
5	17.15290098680092	36.070798299971415	19.165955958959287	27.61034475426838
6	20.01124532527576	35.84754551876151	22.763515061884725	21.377694094078013
7	74.83504100055045	10.80283590093719	8.256100470838934	6.106022627673423
8	75.76632403074038	7.398526295397685	7.067072695357996	9.768076978503947
9	71.82904270624894	10.156938436569042	11.53000210259233	6.4840167545896765
10	34.00765910599664	34.510391294795255	14.696884147162564	16.78506545204554
11	27.90730639022696	28.683611828381217	25.781561918981684	17.62751986241014
12	27.699409620423022	24.400938370420068	31.955859735829357	15.943792273327553
13	24.700971208659844	31.58566173778075	24.351562887591633	19.361804165967772
14	16.05648177241446	34.50991880213661	30.474359004646967	18.95924042080196
15	15.067554637869815	29.907131567943264	32.82926241533522	22.1960513788517
16	16.688913196011217	30.568621290046703	32.12642958560031	20.616035928341763
17	17.24716327220066	26.982638257258078	28.085908615194892	27.68428985534637
18	21.07529879254501	26.862625121962168	27.685943579651628	24.37613250584119
19	22.202902522402056	30.60996439767817	27.70342580802151	19.48370727189826
20	23.047010657071915	30.50318105682433	24.80799079584301	21.641817490260745
21	20.062510778738776	30.01863983538356	27.81139038052196	22.107459005355704
22	18.34476371823373	31.11624028141663	25.955439217363157	24.583556782986484
23	20.020695178448662	30.94307172202312	22.763278815555406	26.272954283972812
24	16.582129855157376	32.171080141842296	27.13123719840203	24.1155528045983
25	18.078041612428446	29.037745076035883	27.57963273145643	25.30458058007924
26	20.6904535220784	31.39217599406549	24.75554411073338	23.161826373122725
27	17.337645616331237	29.374159848991344	27.780914604039342	25.507279930638077
28	19.343613198609926	28.21608034265168	30.6269741333894	21.813332325348995
29	20.00510292071337	29.740341659441466	29.856338607138895	20.39821681270627
30	20.970641668655073	27.034139957050417	28.91678695542268	23.07843141887183
31	22.75052151377198	30.68981565698923	23.27687833550286	23.282784493735928
32	25.365768379373804	26.69370899649647	25.586422450961166	22.354100173168558
33	22.004691852100347	25.35182984594377	27.083987932537497	25.559490369418388
34	20.208511010260178	27.775717184794242	28.4405143555082	23.575257449437377
35	21.739623470600325	25.59374608717017	30.090458719497647	22.576171722731857
36	21.7497820627612	26.432656802594927	30.98134362737339	20.83621750727048
37	21.844516840819587	28.543281508763556	26.098132000274042	23.51406965014281
38	22.80556690850416	26.93822394734542	25.33954503681899	24.91666410733143
39	23.96742635611299	24.955880997998996	26.319258564520055	24.75743408136796
40	24.721524639310914	26.32587346174109	24.437084058806434	24.51551784014156
41	20.10834256662737	25.238195361539574	28.96120126533534	25.692260806497718
42	26.95334371242207	24.174141894270317	26.433838034241543	22.438676359066072
43	21.592678253761633	24.96651208281851	28.521783092795193	24.91902657062466
44	21.79325138735657	23.76378202023686	27.457020886537975	26.9859457058686
45	25.100227505215138	26.08088601823349	25.723209075638987	23.095677400912386
46	20.778809649245076	25.567050251956708	30.721236418789143	22.932903680009073
47	21.3942313371306	25.455305738187096	25.87747792868668	27.272984995995625
48	21.20925046127096	24.252103182946794	32.49213890339179	22.04650745239046
49	24.061216148854086	21.532435439784354	29.732545530573816	24.67380288078774
50	19.725859759453986	24.39833966079752	27.876358121085694	27.9994424586628
51	20.186303855303848	23.29010812994493	31.43611781131951	25.087470203431717
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4480.0
1	3620.0
2	2760.0
3	1830.0
4	900.0
5	1139.0
6	1378.0
7	1246.5
8	1115.0
9	1082.5
10	1050.0
11	1109.5
12	1169.0
13	1142.5
14	1116.0
15	1137.0
16	1158.0
17	1171.0
18	1184.0
19	1193.5
20	1203.0
21	1187.5
22	1172.0
23	1476.5
24	1781.0
25	2188.0
26	3098.5
27	3602.0
28	4143.0
29	4684.0
30	5265.0
31	5846.0
32	6741.5
33	7637.0
34	8663.0
35	9689.0
36	10586.0
37	11483.0
38	12370.5
39	13258.0
40	14257.0
41	15256.0
42	19534.0
43	23812.0
44	33236.5
45	42661.0
46	44642.0
47	46623.0
48	46827.5
49	47032.0
50	43272.5
51	39513.0
52	35081.5
53	30650.0
54	27416.0
55	24182.0
56	20703.5
57	17225.0
58	15687.0
59	14149.0
60	13319.5
61	12490.0
62	11220.5
63	9951.0
64	8070.0
65	6189.0
66	5137.5
67	4086.0
68	3533.0
69	2980.0
70	2616.0
71	2252.0
72	1881.5
73	1511.0
74	1400.5
75	1286.5
76	1283.0
77	867.0
78	451.0
79	342.5
80	234.0
81	165.5
82	97.0
83	79.5
84	62.0
85	45.5
86	29.0
87	20.0
88	11.0
89	7.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	423287.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.669893664158415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.29573881276983	26.69879093846839
2	8.854034555019998	5.962288039326149
3	3.0745990610644958	3.1056427033788854
4	1.4976736475673973	2.017060498288261
5	0.8878924287632176	1.494762183083444
6	0.6306309071337713	1.2739965350715503
7	0.4367679838332913	1.0294152100103044
8	0.3710764221078548	0.9995282938918265
9	0.3203247551381663	0.9706770399149676
>10	3.871001692705284	30.778394947712645
>50	0.6800100953187855	14.88183990543365
>100	0.06828258721109959	3.713112441207294
>500	0.007743386178578304	2.001610785522507
>1k	0.0042236651883154385	5.072880478690139
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCC	4271	1.009008072537073	No Hit
AATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCT	4188	0.9893996272032922	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4010	0.9473477805838592	No Hit
CTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGCT	3941	0.9310467838605958	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGT	3688	0.8712764625419632	No Hit
AAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCT	1305	0.3083014597660689	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	946	0.22348902753923464	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCT	887	0.2095504941091978	No Hit
AATGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT	885	0.20907800145055247	No Hit
AACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTG	879	0.20766052347461653	No Hit
AATGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTC	877	0.2071880308159712	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT	815	0.1925407583979664	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	785	0.18545336851828664	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	670	0.15828504064618096	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTAT	633	0.1495439264612426	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	563	0.13300668340865654	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTCGCAGTC	505	0.11930439630794237	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCT	449	0.10607460186587352	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	433	0.10229466059671098	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.101616633631555	0.0
2	0.0	0.0	0.0	1.5136302319702708	0.0
3	0.0	0.0	0.0	2.161417666972999	0.0
4	0.0	0.0	0.0	5.028739365962101	0.0
5	2.3624632932265816E-4	0.0	0.0	5.611086567742453	0.0
6	2.3624632932265816E-4	0.0	0.0	7.430183303526921	0.0
7	2.3624632932265816E-4	0.0	0.0	8.894910545327402	0.0
8	2.3624632932265816E-4	0.0	0.0	9.892342547727665	0.0
9	2.3624632932265816E-4	0.0	0.0	11.962332885252795	0.0
10	2.3624632932265816E-4	0.0	0.0	13.844743637295736	0.0
11	2.3624632932265816E-4	0.0	0.0	16.676155894227794	0.0
12	2.3624632932265816E-4	0.0	0.0	17.713749772612907	0.0
13	2.3624632932265816E-4	0.0	0.0	18.289245830842905	0.0
14	2.3624632932265816E-4	0.0	0.0	18.89143772428636	0.0
15	2.3624632932265816E-4	0.0	0.0	19.25691079574851	0.0
16	2.3624632932265816E-4	0.0	0.0	19.96872098599768	0.0
17	2.3624632932265816E-4	0.0	0.0	20.696359680311467	0.0
18	2.3624632932265816E-4	0.0	0.0	22.039892555169423	0.0
19	2.3624632932265816E-4	0.0	0.0	22.4859256249306	0.0
20	2.3624632932265816E-4	0.0	0.0	22.96739564409018	0.0
21	2.3624632932265816E-4	0.0	0.0	23.437998332100914	0.0
22	2.3624632932265816E-4	0.0	0.0	23.89891492060942	0.0
23	2.3624632932265816E-4	0.0	0.0	24.33620687618566	0.0
24	2.3624632932265816E-4	0.0	0.0	24.67994528535013	0.0
25	2.3624632932265816E-4	0.0	0.0	24.978560645613967	0.0
26	2.3624632932265816E-4	0.0	0.0	25.25780380687335	0.0
27	2.3624632932265816E-4	0.0	0.0	25.577445090446908	0.0
28	2.3624632932265816E-4	0.0	0.0	25.920238514294084	0.0
29	2.3624632932265816E-4	0.0	0.0	26.201135399858725	0.0
30	2.3624632932265816E-4	0.0	0.0	26.650948410889065	0.0
31	2.3624632932265816E-4	0.0	0.0	26.94200388861458	0.0
32	2.3624632932265816E-4	0.0	0.0	27.25762898458965	0.0
33	2.3624632932265816E-4	0.0	0.0	27.565930444355722	0.0
34	4.724926586453163E-4	0.0	0.0	27.886989205905213	0.0
35	4.724926586453163E-4	0.0	0.0	28.228128905447132	0.0
36	4.724926586453163E-4	0.0	0.0	28.480912477822375	0.0
37	4.724926586453163E-4	0.0	0.0	28.7407834400773	0.0
38	4.724926586453163E-4	0.0	0.0	29.0233340499472	0.0
39	4.724926586453163E-4	0.0	0.0	29.347464013777884	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCCG	30	2.16224E-6	45.000004	1
TAGACGG	25	3.8866146E-5	45.000004	2
TTGTCGC	30	2.16224E-6	45.000004	38
GGTTTCG	30	2.16224E-6	45.000004	8
ACGGGTA	30	2.16224E-6	45.000004	5
CCTCGAC	25	3.8866146E-5	45.000004	30
TAGCACG	30	2.16224E-6	45.000004	1
CCCGTTC	25	3.8866146E-5	45.000004	16
ACGATAA	25	3.8866146E-5	45.000004	34
ATATCCG	25	3.8866146E-5	45.000004	1
CCGTAGT	25	3.8866146E-5	45.000004	27
TACGATA	25	3.8866146E-5	45.000004	33
TCGCTCG	30	2.16224E-6	45.000004	21
GCGGGTC	30	2.16224E-6	45.000004	5
TGCCTAG	30	2.16224E-6	45.000004	1
TCCCGCG	30	2.16224E-6	45.000004	15
CTCGACT	25	3.8866146E-5	45.000004	31
TACTACG	30	2.16224E-6	45.000004	1
TTTGCGG	35	1.2095006E-7	45.000004	2
CGAGACG	25	3.8866146E-5	45.000004	1
>>END_MODULE