FastQCFastQC Report
Sat 18 Jun 2016
SRR3548652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406627
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCC124133.0526748100839343No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGT113072.7806810664318897No Hit
AATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT103992.5573805969598675TruSeq Adapter, Index 27 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT63871.5707269807464828TruSeq Adapter, Index 27 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37360.9187781431139596No Hit
AATGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTT27630.6794925078757682No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT24550.6037474147068443No Hit
AACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG20800.5115253045174079TruSeq Adapter, Index 16 (95% over 23bp)
AAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT20650.5078364201098304TruSeq Adapter, Index 16 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTAT19140.47070165040688394No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTCTCTCTC15220.37429880455552633No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTT14560.3580677131621855No Hit
AATGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC10250.25207376785112645No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8710.21420122126666452No Hit
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC8060.19821605550049554TruSeq Adapter, Index 16 (95% over 24bp)
AAAAAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT8030.19747827861898004No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7320.18001755908978007No Hit
AATGATACGGCTGTCTCTTATACACATCTGAGGCTCTCTCTCTCGTATGCC6200.152473888846535No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATG5820.14312871501400548No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC5810.14288278938683363No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTCTCTCTCTCGTATGCCGT5110.1256679954848055No Hit
AATGATACCTGTCTCTTATACACATCTGACGGTCTCTCTCTCGTATGCCGT4810.11829022666965056No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTA4780.11755244978813507No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT4580.11263393724469846No Hit
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC4530.1114043091088393TruSeq Adapter, Index 16 (95% over 24bp)
AGGCCTGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGAT4470.10992875534580832No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT4430.10894505283712098No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTA253.8863433E-545.00000440
GCCCCAA253.8863433E-545.0000043
CGTATTG302.162029E-645.0000041
CACGGCA253.8863433E-545.0000042
CCGATCA253.8863433E-545.00000417
TCACACG253.8863433E-545.0000041
AGCCCGG351.2093369E-745.0000042
CTATATT253.8863433E-545.00000415
GACGTGT253.8863433E-545.00000428
CCGGAGG351.2093369E-745.0000042
CAACCGG700.045.0000042
GATCACG253.8863433E-545.0000041
CGATTGG351.2093369E-745.0000042
TGACGTG253.8863433E-545.00000427
TATGCAT253.8863433E-545.00000422
ACTAGTC253.8863433E-545.00000444
TCAATCG351.2093369E-745.0000041
GCATTAG253.8863433E-545.0000041
CACTAGT253.8863433E-545.00000443
TCTAACG351.2093369E-745.0000041
Produced by FastQC (version 0.11.5)