##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548652_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	406627
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90704257218532	31.0	31.0	33.0	28.0	34.0
2	31.197842248547193	31.0	31.0	34.0	28.0	34.0
3	31.37228959218153	31.0	31.0	34.0	30.0	34.0
4	35.3497357529136	35.0	35.0	37.0	33.0	37.0
5	34.595961409350586	35.0	35.0	37.0	32.0	37.0
6	34.731631200092465	35.0	35.0	37.0	32.0	37.0
7	34.50351058832788	35.0	35.0	37.0	31.0	37.0
8	34.966891032813855	35.0	35.0	37.0	32.0	37.0
9	36.548023126845976	38.0	35.0	39.0	32.0	39.0
10	36.05787859635489	37.0	35.0	39.0	32.0	39.0
11	36.250667073263706	37.0	35.0	39.0	32.0	39.0
12	35.840802504506584	37.0	35.0	39.0	31.0	39.0
13	35.52112378174593	37.0	35.0	39.0	30.0	39.0
14	36.447766626416836	38.0	35.0	40.0	31.0	41.0
15	36.5928209390916	38.0	35.0	40.0	31.0	41.0
16	36.355967508306136	38.0	35.0	40.0	31.0	41.0
17	36.26458154524909	38.0	35.0	40.0	30.0	41.0
18	36.05764742626535	38.0	35.0	40.0	30.0	41.0
19	36.008929559522606	38.0	34.0	40.0	29.0	41.0
20	36.10934837086568	38.0	34.0	40.0	30.0	41.0
21	36.26914100637685	38.0	34.0	40.0	30.0	41.0
22	35.99348543013622	38.0	34.0	40.0	30.0	41.0
23	35.95103374837382	38.0	34.0	40.0	30.0	41.0
24	35.231794740634534	37.0	34.0	40.0	27.0	41.0
25	35.24355982263844	37.0	34.0	40.0	27.0	41.0
26	35.11504400839098	37.0	33.0	40.0	27.0	41.0
27	34.653571946771855	37.0	33.0	40.0	25.0	41.0
28	34.859701888954746	37.0	33.0	40.0	27.0	41.0
29	34.12767474860253	36.0	32.0	39.0	24.0	41.0
30	34.24160963241496	36.0	32.0	39.0	25.0	41.0
31	34.0760254483839	36.0	32.0	39.0	24.0	40.0
32	33.50770607952743	36.0	31.0	39.0	22.0	41.0
33	33.9921475947244	36.0	32.0	39.0	24.0	41.0
34	33.80594500611125	36.0	32.0	39.0	23.0	41.0
35	34.204398625767595	37.0	33.0	40.0	24.0	41.0
36	34.493462067201634	37.0	33.0	40.0	25.0	41.0
37	34.3681186935447	37.0	33.0	40.0	25.0	41.0
38	34.43788533471707	37.0	33.0	40.0	25.0	41.0
39	34.34808559195528	37.0	33.0	40.0	25.0	41.0
40	34.09152614066454	37.0	33.0	40.0	23.0	41.0
41	34.29198503788484	37.0	33.0	40.0	24.0	41.0
42	34.32126494305592	37.0	33.0	40.0	24.0	41.0
43	34.24234986855275	37.0	33.0	40.0	24.0	41.0
44	34.28996598848576	37.0	33.0	40.0	24.0	41.0
45	33.94608080624258	36.0	33.0	39.0	23.0	40.0
46	33.99778175084291	36.0	33.0	39.0	23.0	40.0
47	33.83609548800252	36.0	33.0	39.0	23.0	40.0
48	34.059745171865124	36.0	33.0	39.0	23.0	40.0
49	33.9963307896426	36.0	33.0	39.0	23.0	40.0
50	33.876761749711655	36.0	33.0	39.0	23.0	40.0
51	32.44020195412504	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	3.0
11	13.0
12	7.0
13	14.0
14	37.0
15	37.0
16	76.0
17	177.0
18	398.0
19	732.0
20	1264.0
21	1878.0
22	2547.0
23	3200.0
24	3632.0
25	4156.0
26	5065.0
27	6085.0
28	7700.0
29	10221.0
30	13603.0
31	18019.0
32	23341.0
33	31069.0
34	44003.0
35	47085.0
36	49634.0
37	56526.0
38	51349.0
39	24751.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.407107742476523	44.5496732878043	15.49134710680796	22.55187186291122
2	18.72502317849036	44.05142796715417	16.754175202335308	20.469373652020156
3	17.934372287132923	28.983564790336107	31.387979647195095	21.694083275335874
4	27.77902106844848	27.698357462736116	17.87362865722148	26.64899281159392
5	14.416898041694232	41.287961694624315	19.85652698910795	24.438613274573502
6	18.9857043432925	31.712601475061913	30.148022634994724	19.15367154665086
7	61.76323756169659	17.87313680596714	12.058471277116373	8.305154355219894
8	63.73260998408862	7.2284919594616195	9.012928310220424	20.025969746229343
9	65.26128368258871	9.055965294975493	16.10271821595713	9.580032806478664
10	35.245323109385254	25.608727408657074	17.966342618665262	21.179606863292403
11	26.974106490715076	23.04372311725488	27.649910114183267	22.332260277846775
12	25.680045840536902	21.366018488688653	36.25140485014522	16.702530820629224
13	23.266531735472558	31.74432588096708	23.36342643257826	21.625715950982105
14	13.079554481133815	30.2215543975191	38.27340535675201	18.42548576459507
15	12.150939312932984	30.07104791368994	30.21073366992354	27.567279103453536
16	13.776261782911611	27.404722263892957	35.18605503323685	23.632960919958588
17	12.622378740221382	27.781234399093023	29.583377395008203	30.01300946567739
18	16.155592225799072	27.014438293571256	27.583510194846873	29.246459285782795
19	17.351282625108514	32.30011779837542	31.79744581643619	18.551153760079877
20	19.49722964780991	33.06396279637112	25.916626293876206	21.522181261942762
21	15.606440300324376	29.58042628748214	28.24111532190435	26.572018090289134
22	15.076224648141908	34.14849481219889	26.986156846446498	23.789123693212698
23	19.953667611840828	28.549751984004995	21.585630073753094	29.910950330401082
24	13.757325509619381	36.397976523939626	25.508881604025312	24.335816362415677
25	16.646213852006873	25.407560245630517	29.343599908515667	28.602625993846942
26	20.357969342911318	31.968364127320616	21.831801626552096	25.84186490321597
27	13.573373140494851	28.556146050311465	31.461511409719474	26.408969399474213
28	19.450503778647263	23.814454032811398	30.955642394627013	25.779399793914326
29	16.65580495146658	28.46121875822314	28.960447781381955	25.922528508928327
30	19.748811564406694	23.87396803458698	29.456233845760366	26.920986555245964
31	21.43045100300767	27.705735231551277	22.92026845241462	27.943545313026437
32	19.962275008791842	21.022460387529605	28.139793963509558	30.875470640169
33	20.653571946771855	21.988948102314897	27.704997454669762	29.652482496243486
34	14.292705599972455	21.107550654531057	32.54014121049512	32.05960253500136
35	15.53315446342717	19.782749300956404	33.43039198085715	31.253704254759274
36	17.403418858068942	18.474179038775095	33.59171919228187	30.53068291087409
37	15.40404350916196	22.91338253485381	32.21576530825548	29.466808647728755
38	19.522314061781437	21.33380223152914	28.698536988443955	30.44534671824547
39	17.01239711086573	19.33344318011347	37.33028057654804	26.323879132472754
40	19.977768323303664	22.081170212504333	24.36188447889589	33.57917698529611
41	16.057467900557512	17.332838203070626	38.35456081371871	28.255133082653145
42	23.08676010200995	19.59092731176238	26.896885843783124	30.42542674244455
43	19.550595508906195	19.455914142445042	31.38306113465166	29.6104292139971
44	19.637407255297852	18.076517299638244	30.223767728163647	32.06230771690026
45	26.36839167099086	23.75715336168036	25.47248461120388	24.4019703561249
46	16.657772356483953	20.190985842061643	39.29621004015966	23.855031761294747
47	21.324948908950955	23.52868845403773	25.148600560218583	29.99776207679274
48	18.99923025278695	17.51703649782233	39.200544971189814	24.283188278200907
49	22.19626340602075	16.22617288079739	31.632675646231068	29.944888066950792
50	19.74684415938932	18.553613016351594	27.222491374158626	34.47705145010046
51	16.570468758837954	18.29219407466794	37.29412950935378	27.84320765714033
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4278.0
1	3400.5
2	2523.0
3	1692.5
4	862.0
5	1052.0
6	1242.0
7	1145.5
8	1049.0
9	1023.0
10	997.0
11	1033.0
12	1069.0
13	1118.0
14	1167.0
15	1169.5
16	1172.0
17	1237.5
18	1303.0
19	1146.5
20	990.0
21	1059.5
22	1129.0
23	1104.5
24	1080.0
25	1532.5
26	1967.5
27	1950.0
28	2600.0
29	3250.0
30	3522.5
31	3795.0
32	4167.5
33	4540.0
34	4774.0
35	5008.0
36	5687.0
37	6366.0
38	7188.0
39	8010.0
40	9884.0
41	11758.0
42	17415.5
43	23073.0
44	41941.5
45	60810.0
46	59394.0
47	57978.0
48	53977.0
49	49976.0
50	44733.5
51	39491.0
52	35041.0
53	30591.0
54	26223.0
55	21855.0
56	18779.0
57	15703.0
58	13874.5
59	12046.0
60	10627.0
61	9208.0
62	7744.5
63	6281.0
64	5320.5
65	4360.0
66	3848.0
67	3336.0
68	2756.0
69	2176.0
70	1736.0
71	1296.0
72	1178.0
73	1060.0
74	840.5
75	488.0
76	355.0
77	327.5
78	300.0
79	201.5
80	103.0
81	144.0
82	185.0
83	130.5
84	76.0
85	83.0
86	90.0
87	81.5
88	73.0
89	53.0
90	33.0
91	24.5
92	16.0
93	10.5
94	5.0
95	4.5
96	4.0
97	3.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	406627.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.156934169342804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.06145627106723	27.004969571832177
2	9.090306157500553	6.209939780018357
3	3.3723360295709943	3.4556597927687798
4	1.6714451539816149	2.283657683688682
5	0.9741924583124135	1.6637713833423668
6	0.6883984017819496	1.4108147335168106
7	0.5496166420656913	1.3141253622989144
8	0.41931048190175324	1.145788842146687
9	0.33830395972500804	1.0399883473399585
>10	3.3727444058293754	25.298127644447156
>50	0.37948712063886275	8.352047243229247
>100	0.06722343279888426	4.765861651178142
>500	0.005782660885925528	1.3594174092828497
>1k	0.006505493496666219	4.695002080443638
>5k	7.22832610740691E-4	1.576934070666466
>10k+	0.0021684978322220726	8.423894403799773
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCC	12413	3.0526748100839343	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGT	11307	2.7806810664318897	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT	10399	2.5573805969598675	TruSeq Adapter, Index 27 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	6387	1.5707269807464828	TruSeq Adapter, Index 27 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3736	0.9187781431139596	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTT	2763	0.6794925078757682	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT	2455	0.6037474147068443	No Hit
AACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	2080	0.5115253045174079	TruSeq Adapter, Index 16 (95% over 23bp)
AAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT	2065	0.5078364201098304	TruSeq Adapter, Index 16 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTAT	1914	0.47070165040688394	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTCTCTCTC	1522	0.37429880455552633	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTT	1456	0.3580677131621855	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC	1025	0.25207376785112645	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	871	0.21420122126666452	No Hit
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	806	0.19821605550049554	TruSeq Adapter, Index 16 (95% over 24bp)
AAAAAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT	803	0.19747827861898004	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	732	0.18001755908978007	No Hit
AATGATACGGCTGTCTCTTATACACATCTGAGGCTCTCTCTCTCGTATGCC	620	0.152473888846535	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATG	582	0.14312871501400548	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC	581	0.14288278938683363	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTCTCTCTCTCGTATGCCGT	511	0.1256679954848055	No Hit
AATGATACCTGTCTCTTATACACATCTGACGGTCTCTCTCTCGTATGCCGT	481	0.11829022666965056	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTA	478	0.11755244978813507	No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	458	0.11263393724469846	No Hit
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	453	0.1114043091088393	TruSeq Adapter, Index 16 (95% over 24bp)
AGGCCTGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGAT	447	0.10992875534580832	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT	443	0.10894505283712098	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.459256271718307E-4	0.0	0.0	1.8660836589798513	0.0
2	2.459256271718307E-4	0.0	0.0	2.417202989471924	0.0
3	2.459256271718307E-4	0.0	0.0	3.583628239147917	0.0
4	2.459256271718307E-4	0.0	0.0	9.270166516242158	0.0
5	2.459256271718307E-4	0.0	0.0	10.046307795596455	0.0
6	2.459256271718307E-4	0.0	0.0	13.312200124438368	0.0
7	2.459256271718307E-4	0.0	0.0	15.860235547565706	0.0
8	2.459256271718307E-4	0.0	0.0	17.269635316887467	0.0
9	2.459256271718307E-4	0.0	0.0	22.080432435622818	0.0
10	2.459256271718307E-4	0.0	0.0	24.633632296920766	0.0
11	2.459256271718307E-4	0.0	0.0	30.657334608867586	0.0
12	2.459256271718307E-4	0.0	0.0	32.14124984322241	0.0
13	2.459256271718307E-4	0.0	0.0	32.89009337796064	0.0
14	2.459256271718307E-4	0.0	0.0	33.95322986422446	0.0
15	2.459256271718307E-4	0.0	0.0	34.486888475187335	0.0
16	2.459256271718307E-4	0.0	0.0	35.34566076527137	0.0
17	2.459256271718307E-4	0.0	0.0	36.18771011270771	0.0
18	2.459256271718307E-4	0.0	0.0	38.11453740159901	0.0
19	2.459256271718307E-4	0.0	0.0	38.71656333691565	0.0
20	2.459256271718307E-4	0.0	0.0	39.24628713784377	0.0
21	2.459256271718307E-4	0.0	0.0	39.822982733561716	0.0
22	2.459256271718307E-4	0.0	0.0	40.29638956586749	0.0
23	2.459256271718307E-4	0.0	0.0	40.77766601824276	0.0
24	2.459256271718307E-4	0.0	0.0	41.103517474245436	0.0
25	2.459256271718307E-4	0.0	0.0	41.40846525193851	0.0
26	2.459256271718307E-4	0.0	0.0	41.68955824379591	0.0
27	4.918512543436614E-4	0.0	0.0	42.0397563368886	0.0
28	4.918512543436614E-4	0.0	0.0	42.34199893268278	0.0
29	4.918512543436614E-4	0.0	0.0	42.62112451952281	0.0
30	4.918512543436614E-4	0.0	0.0	43.12035354268162	0.0
31	4.918512543436614E-4	0.0	0.0	43.41005393149004	0.0
32	4.918512543436614E-4	0.0	0.0	43.70442690721472	0.0
33	4.918512543436614E-4	0.0	0.0	43.99068433724273	0.0
34	4.918512543436614E-4	0.0	0.0	44.26464548591215	0.0
35	4.918512543436614E-4	0.0	0.0	44.55385402346622	0.0
36	4.918512543436614E-4	0.0	0.0	44.81379741138685	0.0
37	4.918512543436614E-4	0.0	0.0	45.08480745253021	0.0
38	4.918512543436614E-4	0.0	0.0	45.33024122844769	0.0
39	4.918512543436614E-4	0.0	0.0	45.580347591281445	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGTA	25	3.8863433E-5	45.000004	40
GCCCCAA	25	3.8863433E-5	45.000004	3
CGTATTG	30	2.162029E-6	45.000004	1
CACGGCA	25	3.8863433E-5	45.000004	2
CCGATCA	25	3.8863433E-5	45.000004	17
TCACACG	25	3.8863433E-5	45.000004	1
AGCCCGG	35	1.2093369E-7	45.000004	2
CTATATT	25	3.8863433E-5	45.000004	15
GACGTGT	25	3.8863433E-5	45.000004	28
CCGGAGG	35	1.2093369E-7	45.000004	2
CAACCGG	70	0.0	45.000004	2
GATCACG	25	3.8863433E-5	45.000004	1
CGATTGG	35	1.2093369E-7	45.000004	2
TGACGTG	25	3.8863433E-5	45.000004	27
TATGCAT	25	3.8863433E-5	45.000004	22
ACTAGTC	25	3.8863433E-5	45.000004	44
TCAATCG	35	1.2093369E-7	45.000004	1
GCATTAG	25	3.8863433E-5	45.000004	1
CACTAGT	25	3.8863433E-5	45.000004	43
TCTAACG	35	1.2093369E-7	45.000004	1
>>END_MODULE