SRR3548648_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548648_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	253804
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC	4508	1.7761737403665818	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	3958	1.5594710879261162	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGT	3792	1.4940662873713575	No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	3103	1.2225969645868466	TruSeq Adapter, Index 19 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2803	1.104395517801138	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT	993	0.39124678886069564	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT	962	0.37903263935950576	No Hit
AAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	941	0.37075853808450615	No Hit
AACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	919	0.3620904319868875	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTAT	737	0.2903815542702243	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	643	0.25334510094403556	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT	618	0.24349498037855985	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTATCTGTC	603	0.2375849080392744	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	573	0.22576476336070356	No Hit
AATGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	458	0.1804542087595152	No Hit
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	381	0.15011583741784998	TruSeq Adapter, Index 13 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT	343	0.1351436541583269	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	342	0.13474964933570785	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	266	0.10480528281666167	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	262	0.10322926352618557	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGGTA	65	0.0	45.000004	5
CGGGTAC	35	1.2069358E-7	45.0	6
TCGTTCC	20	7.022891E-4	45.0	15
CTCACGG	45	3.8198777E-10	45.0	2
CACGGGT	40	6.7793735E-9	45.0	4
GACAATA	20	7.022891E-4	45.0	21
CGCATAG	20	7.022891E-4	45.0	1
GGCAACT	25	3.8821905E-5	45.0	8
CACGCCA	25	3.8821905E-5	45.0	27
CCGTGCG	25	3.8821905E-5	45.0	1
AGGGTAC	40	6.7793735E-9	45.0	6
CGACAAT	20	7.022891E-4	45.0	20
TCTAGCG	25	3.8821905E-5	45.0	1
TAGCCGT	45	3.8198777E-10	45.0	44
TATTGTC	20	7.022891E-4	45.0	18
TTATAGG	25	3.8821905E-5	45.0	2
TTGAGGG	80	0.0	45.0	3
AACGGGC	55	1.8189894E-12	45.0	4
CACTCCA	20	7.022891E-4	45.0	36
ACCTAAG	20	7.022891E-4	45.0	19

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content