##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548648_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	253804
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.91387054577548	31.0	31.0	33.0	28.0	34.0
2	31.211635750421586	31.0	31.0	34.0	28.0	34.0
3	31.395612362295314	31.0	31.0	34.0	30.0	34.0
4	35.36637326440876	37.0	35.0	37.0	33.0	37.0
5	34.65051772233692	35.0	35.0	37.0	32.0	37.0
6	34.76471608012482	35.0	35.0	37.0	32.0	37.0
7	34.58105861215741	35.0	35.0	37.0	31.0	37.0
8	35.08823738002553	36.0	35.0	37.0	32.0	37.0
9	36.52786402105562	38.0	35.0	39.0	32.0	39.0
10	36.01052780886038	37.0	35.0	39.0	31.0	39.0
11	36.19951616207782	37.0	35.0	39.0	32.0	39.0
12	35.90418984728373	37.0	35.0	39.0	31.0	39.0
13	35.59363918614364	37.0	35.0	39.0	30.0	39.0
14	36.569557611385164	38.0	35.0	40.0	31.0	41.0
15	36.64039967849207	38.0	35.0	40.0	31.0	41.0
16	36.445985878867155	38.0	35.0	40.0	31.0	41.0
17	36.38186159398591	38.0	35.0	40.0	31.0	41.0
18	36.23141479251706	38.0	35.0	40.0	30.0	41.0
19	36.217230618902775	38.0	34.0	40.0	30.0	41.0
20	36.18200264771241	38.0	34.0	40.0	30.0	41.0
21	36.311208649193865	38.0	34.0	40.0	30.0	41.0
22	36.24590234984476	38.0	34.0	40.0	30.0	41.0
23	36.16407936833147	38.0	34.0	40.0	30.0	41.0
24	35.682692156151994	38.0	34.0	40.0	29.0	41.0
25	35.919473294353125	38.0	34.0	40.0	30.0	41.0
26	35.82592866936691	38.0	34.0	40.0	29.0	41.0
27	35.691738506879325	38.0	34.0	40.0	29.0	41.0
28	35.65527730059416	38.0	34.0	40.0	29.0	41.0
29	35.22263636506911	38.0	34.0	40.0	27.0	41.0
30	35.355963656995165	38.0	34.0	40.0	27.0	41.0
31	35.12683803249752	38.0	34.0	40.0	27.0	41.0
32	35.234625931821405	38.0	34.0	40.0	27.0	41.0
33	35.15102992860633	38.0	34.0	40.0	27.0	41.0
34	35.10661770500071	38.0	34.0	40.0	26.0	41.0
35	35.119568643520196	38.0	34.0	40.0	26.0	41.0
36	35.095656490835445	38.0	34.0	40.0	26.0	41.0
37	34.943145104096075	37.0	34.0	40.0	26.0	41.0
38	34.75098895210478	37.0	33.0	40.0	25.0	41.0
39	34.768143922081606	37.0	33.0	40.0	25.0	41.0
40	34.551378228869524	37.0	33.0	40.0	24.0	41.0
41	34.65381554270224	37.0	33.0	40.0	25.0	41.0
42	34.59883216970576	37.0	33.0	40.0	25.0	41.0
43	34.56521173819168	37.0	33.0	40.0	25.0	41.0
44	34.47199413720824	37.0	33.0	40.0	24.0	41.0
45	34.191419362973	37.0	33.0	40.0	23.0	41.0
46	34.16321255772171	36.0	33.0	40.0	24.0	41.0
47	34.03239113646751	36.0	33.0	39.0	23.0	41.0
48	34.086582559770534	36.0	33.0	40.0	23.0	41.0
49	33.93831460497076	36.0	33.0	39.0	24.0	40.0
50	33.816819277867964	36.0	33.0	39.0	23.0	40.0
51	32.525220248695845	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	4.0
11	6.0
12	6.0
13	11.0
14	22.0
15	33.0
16	71.0
17	155.0
18	312.0
19	548.0
20	895.0
21	1360.0
22	1765.0
23	2073.0
24	2293.0
25	2607.0
26	2941.0
27	3400.0
28	4056.0
29	5268.0
30	6853.0
31	9021.0
32	12331.0
33	16623.0
34	24842.0
35	27342.0
36	29536.0
37	37529.0
38	40717.0
39	21178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.849584718916958	39.191265701092185	18.8007281209122	23.15842145907866
2	21.789254700477535	37.93084427353391	18.112401695796756	22.167499330191802
3	19.091109675182423	31.32889946572946	25.64577390427259	23.934216954815525
4	23.633591275157208	31.750878630754443	17.632109817024162	26.98342027706419
5	15.825203700493292	39.39220816062788	18.559597169469356	26.222990969409466
6	18.860222849127673	35.49471245528045	24.742320846007154	20.902743849584716
7	69.31963247230146	14.582906494775496	9.309939953665033	6.787521079258011
8	71.4181021575704	8.015240106538904	7.500275803375834	13.066381932514853
9	69.7014231454193	10.103465666419757	12.867409497092245	7.3277016910686985
10	36.719673448803015	28.458574332949837	15.440260988794503	19.38149122945265
11	27.82698460229153	27.29704811586894	24.67218798758097	20.20377929425856
12	27.391609273297508	23.228554317504845	32.108241004869896	17.27159540432775
13	24.62254337993097	31.499503553923503	23.30380923862508	20.574143827520448
14	15.621503207199256	32.29539329561394	33.066854738302	19.01624875888481
15	13.946982711068385	30.309214984791417	31.090132543222325	24.65366976091787
16	15.812595546169486	28.479456588548647	31.93921293596634	23.768734929315535
17	14.632551102425495	27.36166490677846	28.38410742147484	29.62167656932121
18	18.9642401222991	27.36521095018203	26.32976627633922	27.34078265117965
19	20.522529195757354	31.977825408582998	27.943216024964144	19.556429370695497
20	22.710043970938205	31.082646451592566	24.205292272777417	22.002017304691808
21	19.072985453341946	30.32064112464737	26.43969362184993	24.166679800160754
22	17.91579328930986	31.600368788513972	25.010638130210715	25.473199791965456
23	19.673842807835968	29.567697908622403	22.42675450347512	28.33170478006651
24	16.376022442514696	33.164173929488896	25.690690454051158	24.769113173945247
25	19.95279822225024	27.696963010827254	25.75924729318687	26.590991473735638
26	21.739610092827537	30.693369686844967	21.648594978802542	25.918425241524957
27	16.66679800160754	29.734361948590248	27.66465461537249	25.93418543442972
28	23.050858142503664	27.55078722163559	26.61423775827016	22.78411687759058
29	18.944539881168147	29.25210004570456	26.557107058990404	25.246253014136894
30	23.055586200375092	26.96253802146538	25.52639044301902	24.4554853351405
31	23.7742509968322	31.676805724102064	20.82473089470615	23.724212384359586
32	24.001985784305997	24.449969267623835	25.3727285621976	26.175316385872566
33	24.645789664465493	26.44126964114041	23.63713731856078	25.275803375833323
34	19.56233944303478	25.346724243904745	27.78325006698082	27.307686246079655
35	24.739168807426204	23.645411419835778	26.553167010764213	25.06225276197381
36	21.485476982238264	24.857764259034532	29.782036532127155	23.874722226600053
37	20.665946951190683	27.711541189264153	28.409323730122455	23.213188129422704
38	22.91059242565129	28.003498762824858	26.9006792643142	22.18522954720966
39	20.295582417928795	23.597342831476258	31.703598052040157	24.40347669855479
40	24.575262801216688	24.040992261745284	25.241524956265465	26.142219980772563
41	20.92205008589305	21.119052497202564	32.272541015902036	25.686356401002346
42	28.70403933744149	22.522891680194164	24.4885817402405	24.28448724212384
43	21.508723266772787	22.56662621550488	29.250130021591463	26.67452049613087
44	21.96143480796205	21.277442435895416	28.434146033947457	28.32697672219508
45	25.81125592977258	25.868386629052342	26.38650297079636	21.933854470378716
46	19.479598430284785	22.526831728420355	34.67951647728168	23.314053364013176
47	22.015807473483477	25.127263557705948	25.416069092685696	27.440859876124886
48	21.115506453798993	21.01267119509543	34.60268553687097	23.269136814234606
49	23.77464500165482	20.26169800318356	30.50109533340688	25.46256166175474
50	20.436242139603788	22.097366471765614	27.112653858883235	30.353737529747367
51	19.264865801957416	22.22581204393942	32.97308159051867	25.5362405635845
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3250.0
1	2592.0
2	1934.0
3	1297.5
4	661.0
5	742.5
6	824.0
7	765.5
8	707.0
9	677.0
10	647.0
11	663.5
12	680.0
13	708.5
14	737.0
15	770.0
16	803.0
17	779.5
18	756.0
19	789.5
20	823.0
21	797.5
22	772.0
23	855.0
24	938.0
25	1185.5
26	1420.0
27	1407.0
28	1698.0
29	1989.0
30	2083.5
31	2178.0
32	2677.5
33	3177.0
34	3712.0
35	4247.0
36	4567.5
37	4888.0
38	5474.5
39	6061.0
40	7328.5
41	8596.0
42	10734.0
43	12872.0
44	20996.5
45	29121.0
46	29398.0
47	29675.0
48	29160.0
49	28645.0
50	26038.5
51	23432.0
52	20696.5
53	17961.0
54	15946.5
55	13932.0
56	12109.0
57	10286.0
58	9571.5
59	8857.0
60	8241.0
61	7625.0
62	7307.5
63	6990.0
64	6005.5
65	5021.0
66	4191.5
67	3362.0
68	3023.0
69	2684.0
70	2267.5
71	1851.0
72	1656.5
73	1462.0
74	1208.0
75	812.0
76	670.0
77	477.5
78	285.0
79	299.5
80	314.0
81	236.5
82	159.0
83	103.5
84	48.0
85	31.0
86	14.0
87	35.0
88	56.0
89	32.5
90	9.0
91	6.5
92	4.0
93	4.5
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	253804.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.14203085845771
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.96301882114744	30.4995193141164
2	8.364150982377124	6.380514097492554
3	2.8438319938846766	3.2540858300105593
4	1.4348284197260501	2.1890907944713245
5	0.9100675577960043	1.7355912436368222
6	0.622895275086255	1.4255094482356463
7	0.5784765407102865	1.5444989046665931
8	0.45555027580935065	1.3900490141999338
9	0.37600975146168625	1.2907597988999386
>10	4.256967543334091	34.21498479141385
>50	0.13842117224139	3.289152259223653
>100	0.04131975290787761	2.8758412002962914
>500	0.009296944404272462	2.753699705284393
>1k	0.005164969113484701	7.15670359805204
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC	4508	1.7761737403665818	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	3958	1.5594710879261162	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGT	3792	1.4940662873713575	No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	3103	1.2225969645868466	TruSeq Adapter, Index 19 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2803	1.104395517801138	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT	993	0.39124678886069564	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT	962	0.37903263935950576	No Hit
AAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	941	0.37075853808450615	No Hit
AACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	919	0.3620904319868875	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTAT	737	0.2903815542702243	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	643	0.25334510094403556	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT	618	0.24349498037855985	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTATCTGTC	603	0.2375849080392744	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	573	0.22576476336070356	No Hit
AATGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	458	0.1804542087595152	No Hit
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	381	0.15011583741784998	TruSeq Adapter, Index 13 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT	343	0.1351436541583269	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	342	0.13474964933570785	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	266	0.10480528281666167	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	262	0.10322926352618557	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.940048226190289E-4	0.0	0.0	1.4428456604308837	0.0
2	3.940048226190289E-4	0.0	0.0	1.9034372980725285	0.0
3	3.940048226190289E-4	0.0	0.0	2.7332114545082034	0.0
4	3.940048226190289E-4	0.0	0.0	6.418732565286599	0.0
5	3.940048226190289E-4	0.0	0.0	7.021953948716332	0.0
6	7.880096452380578E-4	0.0	0.0	9.27881357267813	0.0
7	7.880096452380578E-4	0.0	0.0	11.174370774298277	0.0
8	7.880096452380578E-4	0.0	0.0	12.340625049250603	0.0
9	7.880096452380578E-4	0.0	0.0	15.23853051961356	0.0
10	7.880096452380578E-4	0.0	0.0	17.276717467021797	0.0
11	7.880096452380578E-4	0.0	0.0	21.102110289829948	0.0
12	7.880096452380578E-4	0.0	0.0	22.229752092165608	0.0
13	7.880096452380578E-4	0.0	0.0	22.836519518998912	0.0
14	7.880096452380578E-4	0.0	0.0	23.595766812185783	0.0
15	7.880096452380578E-4	0.0	0.0	24.011441900048858	0.0
16	7.880096452380578E-4	0.0	0.0	24.67573403098454	0.0
17	7.880096452380578E-4	0.0	0.0	25.489747994515454	0.0
18	7.880096452380578E-4	0.0	0.0	27.059463207829666	0.0
19	7.880096452380578E-4	0.0	0.0	27.5488171975225	0.0
20	7.880096452380578E-4	0.0	0.0	28.09924193472128	0.0
21	7.880096452380578E-4	0.0	0.0	28.57441175079983	0.0
22	7.880096452380578E-4	0.0	0.0	29.042883484893856	0.0
23	7.880096452380578E-4	0.0	0.0	29.538541551748594	0.0
24	7.880096452380578E-4	0.0	0.0	29.834439173535483	0.0
25	7.880096452380578E-4	0.0	0.0	30.10748451561047	0.0
26	7.880096452380578E-4	0.0	0.0	30.349009471875934	0.0
27	7.880096452380578E-4	0.0	0.0	30.685095585569968	0.0
28	7.880096452380578E-4	0.0	0.0	30.971143086791383	0.0
29	7.880096452380578E-4	0.0	0.0	31.269010732691367	0.0
30	7.880096452380578E-4	0.0	0.0	31.75127263557706	0.0
31	7.880096452380578E-4	0.0	0.0	32.03022804999133	0.0
32	7.880096452380578E-4	0.0	0.0	32.30563742100203	0.0
33	7.880096452380578E-4	0.0	0.0	32.565680603930595	0.0
34	7.880096452380578E-4	0.0	0.0	32.81784369040677	0.0
35	7.880096452380578E-4	0.0	0.0	33.109013254322235	0.0
36	7.880096452380578E-4	0.0	0.0	33.363540369734125	0.0
37	7.880096452380578E-4	0.0	0.0	33.62358355266269	0.0
38	7.880096452380578E-4	0.0	0.0	33.86944256197696	0.0
39	7.880096452380578E-4	0.0	0.0	34.14485193298766	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGGTA	65	0.0	45.000004	5
CGGGTAC	35	1.2069358E-7	45.0	6
TCGTTCC	20	7.022891E-4	45.0	15
CTCACGG	45	3.8198777E-10	45.0	2
CACGGGT	40	6.7793735E-9	45.0	4
GACAATA	20	7.022891E-4	45.0	21
CGCATAG	20	7.022891E-4	45.0	1
GGCAACT	25	3.8821905E-5	45.0	8
CACGCCA	25	3.8821905E-5	45.0	27
CCGTGCG	25	3.8821905E-5	45.0	1
AGGGTAC	40	6.7793735E-9	45.0	6
CGACAAT	20	7.022891E-4	45.0	20
TCTAGCG	25	3.8821905E-5	45.0	1
TAGCCGT	45	3.8198777E-10	45.0	44
TATTGTC	20	7.022891E-4	45.0	18
TTATAGG	25	3.8821905E-5	45.0	2
TTGAGGG	80	0.0	45.0	3
AACGGGC	55	1.8189894E-12	45.0	4
CACTCCA	20	7.022891E-4	45.0	36
ACCTAAG	20	7.022891E-4	45.0	19
>>END_MODULE