SRR3548647_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548647_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	257398
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC	4793	1.8620968305892043	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	4104	1.5944179830457113	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGT	3975	1.5443010435201516	No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	3197	1.2420453927380943	TruSeq Adapter, Index 19 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2752	1.0691613765452723	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT	1026	0.3986044957614278	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT	1011	0.39277694465380464	No Hit
AAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	965	0.37490578792376006	No Hit
AACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	940	0.36519320274438805	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTAT	775	0.3010901405605327	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	672	0.26107428962151996	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT	625	0.24281462948430058	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	567	0.22028143186815752	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTATCTGTC	557	0.21639639779640865	No Hit
AATGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	471	0.1829851047793689	No Hit
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	402	0.15617836968430215	TruSeq Adapter, Index 13 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT	327	0.12704061414618606	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	318	0.12354408348161214	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	300	0.11655102215246428	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	277	0.107615443787442	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACAA	30	2.1589149E-6	45.000004	8
CAAGTAG	35	1.2070268E-7	45.000004	1
ATAGGGC	60	0.0	45.000004	4
GGGTACC	30	2.1589149E-6	45.000004	7
ATATGGG	60	0.0	45.000004	3
TCGATGT	30	2.1589149E-6	45.000004	35
ACCTCGA	30	2.1589149E-6	45.000004	32
CCCACGG	30	2.1589149E-6	45.000004	2
TGACACG	30	2.1589149E-6	45.000004	1
AAGGGCG	35	1.2070268E-7	45.000004	5
CGTCATC	30	2.1589149E-6	45.000004	38
TTTTAGC	30	2.1589149E-6	45.000004	12
TAGTAGG	30	2.1589149E-6	45.000004	2
TTGGATA	20	7.023073E-4	45.0	44
CATGCGG	25	3.8823426E-5	45.0	2
TCACATC	20	7.023073E-4	45.0	18
GCTATTC	25	3.8823426E-5	45.0	39
GGCCGTA	20	7.023073E-4	45.0	42
ATAGCGG	20	7.023073E-4	45.0	2
GGATCTA	20	7.023073E-4	45.0	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content