##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548647_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	257398
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.010823704923894	31.0	31.0	33.0	28.0	34.0
2	31.3069254617363	31.0	31.0	34.0	30.0	34.0
3	31.486651022929472	31.0	31.0	34.0	30.0	34.0
4	35.427509149255236	37.0	35.0	37.0	33.0	37.0
5	34.68724698715608	35.0	35.0	37.0	32.0	37.0
6	34.819116698653445	35.0	35.0	37.0	32.0	37.0
7	34.661664037793614	35.0	35.0	37.0	32.0	37.0
8	35.10101088586547	36.0	35.0	37.0	32.0	37.0
9	36.60823704923892	38.0	35.0	39.0	32.0	39.0
10	36.082747340694176	37.0	35.0	39.0	31.0	39.0
11	36.22234826999433	37.0	35.0	39.0	32.0	39.0
12	35.89092766843565	37.0	35.0	39.0	31.0	39.0
13	35.6750324400345	37.0	35.0	39.0	30.0	39.0
14	36.62672592638638	38.0	35.0	40.0	31.0	41.0
15	36.68085610610805	38.0	35.0	40.0	31.0	41.0
16	36.488531379420195	38.0	35.0	40.0	31.0	41.0
17	36.429785779221284	38.0	35.0	40.0	31.0	41.0
18	36.24188222130708	38.0	35.0	40.0	30.0	41.0
19	36.290390756726936	38.0	34.0	40.0	30.0	41.0
20	36.223253482933046	38.0	34.0	40.0	30.0	41.0
21	36.338623454727696	38.0	34.0	40.0	30.0	41.0
22	36.29532474999806	38.0	34.0	40.0	30.0	41.0
23	36.17435255907194	38.0	34.0	40.0	30.0	41.0
24	35.714383950147244	38.0	34.0	40.0	29.0	41.0
25	35.93423802826751	38.0	34.0	40.0	30.0	41.0
26	35.780386793992186	38.0	34.0	40.0	29.0	41.0
27	35.60885476965633	38.0	34.0	40.0	28.0	41.0
28	35.53268090661155	38.0	34.0	40.0	27.0	41.0
29	35.21926743797543	38.0	34.0	40.0	27.0	41.0
30	35.36235712787201	38.0	34.0	40.0	27.0	41.0
31	35.10653928934957	38.0	34.0	40.0	26.0	41.0
32	35.22977257010544	38.0	34.0	40.0	27.0	41.0
33	35.13074305161656	38.0	34.0	40.0	27.0	41.0
34	35.097013185805636	38.0	34.0	40.0	26.0	41.0
35	35.09212192790931	38.0	34.0	40.0	26.0	41.0
36	35.024530105129024	38.0	34.0	40.0	26.0	41.0
37	34.89548092836774	37.0	33.0	40.0	25.0	41.0
38	34.73321859532708	37.0	33.0	40.0	25.0	41.0
39	34.780060451130154	37.0	33.0	40.0	25.0	41.0
40	34.52575777589569	37.0	33.0	40.0	24.0	41.0
41	34.63096061352458	37.0	33.0	40.0	25.0	41.0
42	34.57455768887093	37.0	33.0	40.0	25.0	41.0
43	34.559732398853136	37.0	33.0	40.0	25.0	41.0
44	34.42143295596702	37.0	33.0	40.0	24.0	41.0
45	34.143136310305444	36.0	33.0	40.0	23.0	41.0
46	34.101772352543534	36.0	33.0	40.0	23.0	41.0
47	33.95215969044048	36.0	33.0	39.0	23.0	40.0
48	34.00727666881639	36.0	33.0	39.0	23.0	40.0
49	33.823786509607686	36.0	33.0	39.0	23.0	40.0
50	33.720724325752336	36.0	33.0	39.0	23.0	40.0
51	32.33766385131197	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	6.0
12	10.0
13	8.0
14	23.0
15	38.0
16	77.0
17	151.0
18	294.0
19	544.0
20	923.0
21	1283.0
22	1815.0
23	2055.0
24	2307.0
25	2556.0
26	2946.0
27	3545.0
28	4299.0
29	5516.0
30	7057.0
31	9383.0
32	12478.0
33	16677.0
34	25171.0
35	27635.0
36	29791.0
37	37821.0
38	41243.0
39	21737.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.732468783751234	39.58655467408449	18.66448068749563	23.016495854668644
2	21.835445496857005	38.145984040280034	18.09066115509833	21.92790930776463
3	18.959354773541364	31.40273040194563	25.868110863332273	23.769803961180738
4	23.74688226015742	31.647099045058624	17.444579988966503	27.16143870581745
5	15.95428091904366	39.28663004374548	18.42399707845438	26.33509195875648
6	18.866890962633743	35.28854148050878	24.82381370484619	21.020753852011282
7	69.23014164834225	14.57004327927956	9.39478939230297	6.8050256800752145
8	71.38944358542024	7.9126488939308	7.493453717589103	13.204453803059854
9	69.59222682382924	10.03465450392	12.976013799641024	7.3971048726097335
10	36.7003628621823	28.331610968228194	15.397167033154883	19.570859136434628
11	27.80013830721295	27.433391090839866	24.676959416934086	20.08951118501309
12	27.242247414509823	23.05612320220048	32.42993341051601	17.27169597277368
13	24.454346964622882	31.282682849128584	23.51533422948119	20.74763595676734
14	15.651248261447254	32.00335666943799	33.52900954941374	18.816385519701008
15	13.955042385721722	30.243824738342955	31.13077801692321	24.670354859012114
16	15.873860713758459	28.115603073838958	32.06940224865772	23.94113396374486
17	14.507494230724404	27.51730782678964	28.70807076978065	29.267127172705305
18	18.791521301641815	27.45825530889906	26.45203148431612	27.29819190514301
19	20.174981934591564	32.02588986705413	28.23448511643447	19.564643081919826
20	22.584868569297353	31.33008026480392	24.293895057459654	21.791156108439072
21	18.916230895344953	30.103963511759996	26.53866774411612	24.441137848778936
22	17.82259380414766	31.703432039098985	25.056527245743943	25.41744691100941
23	19.68352512451534	29.51382683626135	22.27989339466507	28.522754644558233
24	16.281789291292085	33.28658342333662	25.545653035377118	24.885974249994174
25	20.054157374960177	27.438830138540315	25.70649344594752	26.800519040551983
26	21.643524813712617	30.830853386584202	21.502498076908136	26.02312372279505
27	16.7207981413997	29.528589965734	27.933783479281114	25.816828413585185
28	23.098470073582543	27.47884598947933	26.49515536251253	22.927528574425597
29	18.93526756229652	29.217010233179746	26.601216792671273	25.24650541185246
30	23.269411572739493	26.828491285868576	25.48116146978609	24.42093567160584
31	23.909665187763697	31.7247997264936	20.67848235029021	23.68705273545249
32	24.077498659663245	24.252713696299118	25.31410500470089	26.35568263933675
33	24.758156629033635	26.337811482606703	23.602358992688366	25.301672895671295
34	19.47334478123373	25.299341875228247	27.8133474230569	27.41396592048112
35	25.355286365861428	23.30088034872066	26.420174204927775	24.923659080490136
36	21.330779570936837	24.839353841133185	29.852601807317853	23.977264780612124
37	20.680036363918912	27.48778156784435	28.64901825189007	23.18316381634667
38	22.971040956029185	27.86346436258246	26.83354183016185	22.331952851226504
39	20.238696493368245	23.51999627036729	31.901180273351	24.340126962913462
40	24.648987171617495	23.985423352162798	25.16064615886681	26.204943317352893
41	20.990839089658817	20.900317795787068	32.53599484067475	25.572848273879362
42	28.930294718684685	22.328456320561934	24.373538255930505	24.36771070482288
43	21.617883588839074	22.411207546290182	29.531309489584224	26.43959937528652
44	21.9422839338301	21.17926324213863	28.433010357500837	28.44544246653043
45	26.0099146069511	25.759329909323302	26.33120692468473	21.899548559040863
46	19.40302566453508	22.448115369971795	34.64362582459849	23.505233140894646
47	22.02348114592965	25.265153575396855	25.300895888856946	27.410469389816548
48	21.19091834435388	20.761233576018462	34.683641675537494	23.36420640409016
49	23.826913962035448	20.09650424634224	30.523935694916045	25.552646096706265
50	20.617487315363757	21.83816502070723	27.09306210615467	30.451285557774344
51	19.394867092984406	21.999393934684807	33.05658940628909	25.549149566041695
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3177.0
1	2561.5
2	1946.0
3	1299.0
4	652.0
5	749.0
6	846.0
7	764.5
8	683.0
9	666.5
10	650.0
11	671.0
12	692.0
13	704.5
14	717.0
15	740.5
16	764.0
17	758.0
18	752.0
19	764.5
20	777.0
21	774.0
22	771.0
23	843.5
24	916.0
25	1174.0
26	1455.5
27	1479.0
28	1745.5
29	2012.0
30	2104.5
31	2197.0
32	2720.5
33	3244.0
34	3765.5
35	4287.0
36	4637.0
37	4987.0
38	5559.0
39	6131.0
40	7415.5
41	8700.0
42	11000.0
43	13300.0
44	21631.0
45	29962.0
46	30225.0
47	30488.0
48	29778.0
49	29068.0
50	26415.5
51	23763.0
52	20845.5
53	17928.0
54	15990.0
55	14052.0
56	12141.0
57	10230.0
58	9491.0
59	8752.0
60	8258.0
61	7764.0
62	7383.0
63	7002.0
64	5985.5
65	4969.0
66	4223.0
67	3477.0
68	3118.0
69	2759.0
70	2356.5
71	1954.0
72	1722.0
73	1490.0
74	1205.5
75	815.0
76	709.0
77	501.0
78	293.0
79	322.0
80	351.0
81	267.5
82	184.0
83	118.5
84	53.0
85	39.5
86	26.0
87	47.0
88	68.0
89	39.0
90	10.0
91	8.5
92	7.0
93	4.0
94	1.0
95	2.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	257398.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.65375022338946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.75856376392902	30.032090381432646
2	8.388361535286833	6.317065400663564
3	2.8900123813454393	3.2645941304905244
4	1.5260008254230293	2.2983861568465955
5	0.9430458109781263	1.7754605707892057
6	0.6675608749484111	1.5081702266528878
7	0.5148576145274453	1.3570424012618592
8	0.4219975237309121	1.2711831482762104
9	0.38382170862567067	1.3007094072215013
>10	4.315930664465538	34.78776058866036
>50	0.1361947998349154	3.238564402209807
>100	0.03920759389186958	2.763813238642103
>500	0.007222451506397028	1.9817558799990678
>1k	0.007222451506397028	8.103404066853665
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC	4793	1.8620968305892043	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	4104	1.5944179830457113	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGT	3975	1.5443010435201516	No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	3197	1.2420453927380943	TruSeq Adapter, Index 19 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2752	1.0691613765452723	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT	1026	0.3986044957614278	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT	1011	0.39277694465380464	No Hit
AAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	965	0.37490578792376006	No Hit
AACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	940	0.36519320274438805	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTAT	775	0.3010901405605327	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	672	0.26107428962151996	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT	625	0.24281462948430058	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	567	0.22028143186815752	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTATCTGTC	557	0.21639639779640865	No Hit
AATGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	471	0.1829851047793689	No Hit
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	402	0.15617836968430215	TruSeq Adapter, Index 13 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT	327	0.12704061414618606	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	318	0.12354408348161214	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	300	0.11655102215246428	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	277	0.107615443787442	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.770068143497619E-4	0.0	0.0	1.498069138066341	0.0
2	7.770068143497619E-4	0.0	0.0	1.9732088050412202	0.0
3	7.770068143497619E-4	0.0	0.0	2.8150956883891873	0.0
4	7.770068143497619E-4	0.0	0.0	6.555217989261766	0.0
5	7.770068143497619E-4	0.0	0.0	7.123209970551442	0.0
6	7.770068143497619E-4	0.0	0.0	9.41538007288324	0.0
7	7.770068143497619E-4	0.0	0.0	11.322154795297555	0.0
8	7.770068143497619E-4	0.0	0.0	12.490773044079596	0.0
9	7.770068143497619E-4	0.0	0.0	15.466709143039184	0.0
10	7.770068143497619E-4	0.0	0.0	17.507517540928834	0.0
11	7.770068143497619E-4	0.0	0.0	21.50599460757271	0.0
12	7.770068143497619E-4	0.0	0.0	22.700642584635467	0.0
13	7.770068143497619E-4	0.0	0.0	23.327687083815725	0.0
14	7.770068143497619E-4	0.0	0.0	24.119457027638134	0.0
15	7.770068143497619E-4	0.0	0.0	24.547587782344852	0.0
16	7.770068143497619E-4	0.0	0.0	25.224360717643492	0.0
17	7.770068143497619E-4	0.0	0.0	26.05342698855469	0.0
18	7.770068143497619E-4	0.0	0.0	27.610548644511614	0.0
19	7.770068143497619E-4	0.0	0.0	28.067817154756447	0.0
20	7.770068143497619E-4	0.0	0.0	28.592296754442536	0.0
21	7.770068143497619E-4	0.0	0.0	29.064328394160018	0.0
22	7.770068143497619E-4	0.0	0.0	29.519654387368977	0.0
23	7.770068143497619E-4	0.0	0.0	29.99285153730798	0.0
24	7.770068143497619E-4	0.0	0.0	30.29277616764699	0.0
25	7.770068143497619E-4	0.0	0.0	30.562397532226356	0.0
26	7.770068143497619E-4	0.0	0.0	30.797830596974336	0.0
27	7.770068143497619E-4	0.0	0.0	31.128835499887334	0.0
28	7.770068143497619E-4	0.0	0.0	31.391852306544727	0.0
29	7.770068143497619E-4	0.0	0.0	31.673905780153692	0.0
30	7.770068143497619E-4	0.0	0.0	32.11524565070436	0.0
31	7.770068143497619E-4	0.0	0.0	32.37321191306848	0.0
32	7.770068143497619E-4	0.0	0.0	32.64594130490524	0.0
33	7.770068143497619E-4	0.0	0.0	32.92566375807116	0.0
34	7.770068143497619E-4	0.0	0.0	33.15876580237609	0.0
35	7.770068143497619E-4	0.0	0.0	33.433049207841556	0.0
36	7.770068143497619E-4	0.0	0.0	33.65915819081734	0.0
37	7.770068143497619E-4	0.0	0.0	33.90663486118773	0.0
38	7.770068143497619E-4	0.0	0.0	34.13585187142091	0.0
39	7.770068143497619E-4	0.0	0.0	34.39265262356351	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACAA	30	2.1589149E-6	45.000004	8
CAAGTAG	35	1.2070268E-7	45.000004	1
ATAGGGC	60	0.0	45.000004	4
GGGTACC	30	2.1589149E-6	45.000004	7
ATATGGG	60	0.0	45.000004	3
TCGATGT	30	2.1589149E-6	45.000004	35
ACCTCGA	30	2.1589149E-6	45.000004	32
CCCACGG	30	2.1589149E-6	45.000004	2
TGACACG	30	2.1589149E-6	45.000004	1
AAGGGCG	35	1.2070268E-7	45.000004	5
CGTCATC	30	2.1589149E-6	45.000004	38
TTTTAGC	30	2.1589149E-6	45.000004	12
TAGTAGG	30	2.1589149E-6	45.000004	2
TTGGATA	20	7.023073E-4	45.0	44
CATGCGG	25	3.8823426E-5	45.0	2
TCACATC	20	7.023073E-4	45.0	18
GCTATTC	25	3.8823426E-5	45.0	39
GGCCGTA	20	7.023073E-4	45.0	42
ATAGCGG	20	7.023073E-4	45.0	2
GGATCTA	20	7.023073E-4	45.0	8
>>END_MODULE