FastQCFastQC Report
Sat 18 Jun 2016
SRR3548643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235525
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGT42641.810423521919117No Hit
AATCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT42181.7908926865513215No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCC41251.7514064324381702No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20010.8495913384990977No Hit
CTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGCT19410.8241163358454517No Hit
AATGACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTT10520.4466617131939285No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT10180.4322258783568623No Hit
AACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG6930.29423628064961255No Hit
AATGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTC6060.2572975268018257No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTAT4890.2076212716272158No Hit
AAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT4770.20252627109648658No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4340.18426918586137353No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4330.18384460248381276No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTT3360.1426600148604182No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG2960.1256766797579875No Hit
AATCTGACTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT2570.1091179280331175No Hit
AATCTGTCACTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT2440.10359834412482752No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG207.021853E-445.0000041
ACACGAC207.021853E-445.00000426
ACTGCCG302.158129E-645.0000041
CTCGTGT207.021853E-445.00000430
CGTATGG207.021853E-445.0000042
CGACGTT351.2064265E-745.00000427
GGCCGGT207.021853E-445.0000049
ATTCGGG207.021853E-445.0000043
ATGCTAG302.158129E-645.0000041
ATTCGAC207.021853E-445.00000421
GTCTACT207.021853E-445.00000416
ACTTAGC207.021853E-445.00000436
TAGCATA207.021853E-445.00000430
CCCGTGA207.021853E-445.00000425
GTTAATC207.021853E-445.00000440
TACGAAG207.021853E-445.0000041
ACCAGAC207.021853E-445.00000442
GACGGTT207.021853E-445.00000425
GACGGCT207.021853E-445.00000423
CGTAATG207.021853E-445.0000041