##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548643_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	235525
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.974134380638997	31.0	31.0	33.0	28.0	34.0
2	31.17107738032056	31.0	31.0	34.0	28.0	34.0
3	31.340757881328948	31.0	31.0	34.0	28.0	34.0
4	35.3025241481796	37.0	35.0	37.0	33.0	37.0
5	34.921341683473095	35.0	35.0	37.0	32.0	37.0
6	34.93883027279482	35.0	35.0	37.0	32.0	37.0
7	32.47307929094576	35.0	35.0	37.0	25.0	37.0
8	33.8957010933022	35.0	35.0	37.0	27.0	37.0
9	34.22569578600998	37.0	34.0	39.0	28.0	39.0
10	34.83405583271415	37.0	34.0	39.0	27.0	39.0
11	35.77089056363443	37.0	35.0	39.0	30.0	39.0
12	35.649846088525635	37.0	35.0	39.0	30.0	39.0
13	35.66698651947776	37.0	35.0	39.0	30.0	39.0
14	36.65061458443902	38.0	35.0	40.0	31.0	41.0
15	36.841948837703	38.0	35.0	40.0	31.0	41.0
16	36.742923256554505	38.0	35.0	40.0	31.0	41.0
17	36.70043095212822	38.0	35.0	40.0	31.0	41.0
18	36.47406007854792	38.0	35.0	40.0	31.0	41.0
19	36.435902770406535	38.0	35.0	40.0	30.0	41.0
20	36.489446980150724	38.0	35.0	40.0	31.0	41.0
21	36.645273325549304	38.0	35.0	40.0	31.0	41.0
22	36.58623076106571	38.0	35.0	40.0	31.0	41.0
23	36.57742914764887	38.0	35.0	40.0	31.0	41.0
24	36.280866150090226	38.0	35.0	40.0	30.0	41.0
25	36.31436153274599	38.0	35.0	40.0	30.0	41.0
26	36.251378834518626	38.0	35.0	40.0	30.0	41.0
27	36.151007324063265	38.0	35.0	40.0	30.0	41.0
28	36.074696953614264	38.0	35.0	40.0	30.0	41.0
29	35.83556310370449	38.0	34.0	40.0	29.0	41.0
30	35.83811060396985	38.0	34.0	40.0	29.0	41.0
31	35.718140324806285	38.0	34.0	40.0	29.0	41.0
32	35.67938859993631	38.0	34.0	40.0	29.0	41.0
33	35.613183313873265	38.0	34.0	40.0	29.0	41.0
34	35.658146693556944	38.0	34.0	40.0	29.0	41.0
35	35.48469164632205	38.0	34.0	40.0	28.0	41.0
36	35.4504787177582	38.0	34.0	40.0	28.0	41.0
37	35.336533276722214	38.0	34.0	40.0	27.0	41.0
38	35.16325655450589	38.0	34.0	40.0	27.0	41.0
39	35.19234900753636	38.0	34.0	40.0	27.0	41.0
40	34.846339029826986	37.0	34.0	40.0	25.0	41.0
41	34.96203800021229	38.0	34.0	40.0	26.0	41.0
42	34.89325124721367	37.0	34.0	40.0	26.0	41.0
43	34.8884067508757	37.0	34.0	40.0	26.0	41.0
44	34.77667338923681	37.0	34.0	40.0	25.0	41.0
45	34.525090754696954	37.0	33.0	40.0	24.0	41.0
46	34.47838658316527	37.0	33.0	40.0	24.0	41.0
47	34.34494427343169	37.0	33.0	40.0	24.0	41.0
48	34.294295722322474	37.0	33.0	40.0	24.0	41.0
49	34.14053285213884	37.0	33.0	40.0	23.0	41.0
50	33.916815624668295	36.0	33.0	40.0	23.0	41.0
51	31.72480628383399	35.0	30.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	5.0
11	15.0
12	12.0
13	14.0
14	17.0
15	13.0
16	39.0
17	93.0
18	190.0
19	341.0
20	602.0
21	893.0
22	1320.0
23	1758.0
24	2046.0
25	2321.0
26	2704.0
27	3218.0
28	4066.0
29	5144.0
30	6635.0
31	8571.0
32	11435.0
33	15175.0
34	22788.0
35	24841.0
36	26605.0
37	35343.0
38	38070.0
39	21247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.3809574355164	39.34359409829105	18.97208364292538	22.30336482326717
2	21.498779322789513	37.58157308141386	19.007748646640486	21.911898949156143
3	19.64165162933871	30.463432756607578	26.88546863390298	23.009446980150727
4	24.73028340940452	30.463432756607578	18.44814775501539	26.35813607897251
5	16.257297526801825	38.19891731238722	18.472773590913917	27.071011569897042
6	19.912111240844922	35.582634539857764	24.915401762021016	19.589852457276297
7	67.29264409298375	18.837490712238615	8.21526377242331	5.654601422354315
8	73.84651310901178	7.805965396454728	6.398046916463221	11.949474578070268
9	67.18310158157308	14.768708205073771	11.176732830909671	6.871457382443477
10	37.672433924211866	29.688568092559176	15.401337437639317	17.23766054558964
11	29.980681456320983	26.418426918586135	25.101793864770194	18.499097760322684
12	29.02367052329901	23.828043732087888	30.963591975374165	16.184693769238933
13	25.249973463538904	30.508438594629023	23.34401868166861	20.897569260163465
14	15.997027916357073	33.83844602483813	31.815306230761063	18.34921982804373
15	16.340940452181297	31.477762445600256	30.09022396773166	22.091073134486784
16	17.190107207302834	27.53975161872413	33.29922513533595	21.970916038637085
17	16.225878356862328	28.279800445812548	27.615327459929944	27.87899373739518
18	19.419594522874426	29.40239889608322	26.56405901708948	24.61394756395287
19	21.443583483706615	32.03948625411315	27.923574992039065	18.593355270141174
20	23.394119520220784	31.325761596433498	25.192654707568195	20.08746417577752
21	21.162084704383822	29.04659802568729	25.567561829954354	24.223755439974525
22	19.21112408449209	31.173760747266744	26.390404415667128	23.224710752574037
23	22.109754803099456	29.01687718925804	22.09998938541556	26.77337862222694
24	17.859675193716164	34.08725188408874	26.085128967200934	21.967943954994162
25	19.16951491349114	26.82560237766691	27.70194246895234	26.30294023988961
26	22.144995223437004	32.04076000424583	22.212503980469165	23.601740791848
27	16.604606729646534	30.04182146268974	28.68527757138308	24.668294236280648
28	21.372253476276406	26.952128224180022	30.600997770937266	21.074620528606307
29	18.45961150620953	28.96168135017514	27.976223330856598	24.60248381275873
30	21.08183844602484	27.81912748115911	28.788875915507905	22.31015815730814
31	26.544528181721688	29.427024731981742	20.882708841948837	23.145738244347733
32	21.087782613310686	26.200615645897464	24.37575628914128	28.335845451650567
33	24.614796730707994	28.213990022290623	26.099564802038	21.07164844496338
34	18.817535293493258	25.595584332873365	23.89003290521176	31.696847468421613
35	19.118989491561404	25.736546014223542	32.07217917418533	23.07228532002972
36	24.583377560768497	24.405477125570535	27.852669567986414	23.158475745674558
37	19.049782401019	27.9881116654283	26.12758730495701	26.83451862859569
38	23.96221207939709	28.542192973145102	26.013374376393166	21.48222057106464
39	20.26451544422036	25.748434348795247	26.013374376393166	27.973675830591233
40	21.73951809786647	29.26568304850865	25.51363974100414	23.48115911262074
41	19.157626578919434	22.244772317163783	31.860736652160064	26.736864451756716
42	23.709160386370872	24.01698333510243	26.621377773060185	25.652478505466508
43	21.868166861267383	24.477656299755864	27.027279482008282	26.626897356968477
44	20.593567561829953	23.869652903088845	27.372890351342743	28.163889183738455
45	25.1514701199448	27.968580830060503	24.944273431695148	21.935675618299545
46	19.1915932491243	25.33191805540813	33.19987262498673	22.27661607048084
47	21.715316845345505	27.046810317376075	24.54941089056363	26.688461946714785
48	20.61946714786116	22.56320985033436	33.52807557584121	23.289247425963275
49	20.930262180235644	21.88260269610445	31.37586243498567	25.811272688674237
50	20.54686339029827	24.12610126313555	26.489756926016344	28.837278420549833
51	18.36450482963592	24.568092559176307	32.71797049145526	24.349432119732512
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2305.0
1	1820.0
2	1335.0
3	902.5
4	470.0
5	445.5
6	421.0
7	446.0
8	471.0
9	510.5
10	550.0
11	579.0
12	608.0
13	624.5
14	641.0
15	631.0
16	621.0
17	653.0
18	685.0
19	692.5
20	700.0
21	726.0
22	752.0
23	876.5
24	1001.0
25	1158.5
26	1555.0
27	1794.0
28	2107.5
29	2421.0
30	2643.0
31	2865.0
32	3372.0
33	3879.0
34	4511.5
35	5144.0
36	5684.0
37	6224.0
38	6999.5
39	7775.0
40	9202.5
41	10630.0
42	12847.0
43	15064.0
44	22984.5
45	30905.0
46	29668.0
47	28431.0
48	25961.0
49	23491.0
50	20702.0
51	17913.0
52	16187.0
53	14461.0
54	12580.0
55	10699.0
56	9793.0
57	8887.0
58	8371.0
59	7855.0
60	7273.0
61	6691.0
62	5997.0
63	5303.0
64	4587.0
65	3871.0
66	3324.0
67	2777.0
68	2405.0
69	2033.0
70	1905.5
71	1778.0
72	1463.0
73	1148.0
74	863.5
75	514.0
76	449.0
77	369.0
78	289.0
79	201.0
80	113.0
81	92.0
82	71.0
83	55.5
84	40.0
85	34.0
86	28.0
87	21.5
88	15.0
89	11.0
90	7.0
91	6.5
92	6.0
93	6.0
94	6.0
95	4.0
96	2.0
97	2.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	235525.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.84470862965715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.19856704196519	27.24806283833988
2	8.776867963152506	6.116548137140431
3	3.2607106302090947	3.4085553550578496
4	1.7509869864015208	2.4405052542192975
5	1.032070965540771	1.7981106039698547
6	0.693327484525028	1.4495276509924637
7	0.5483257786226057	1.337437639316421
8	0.3935760588780036	1.0971234476170257
9	0.34118048447628796	1.0699501114531365
>10	4.679046644246235	36.84322258783569
>50	0.2717258858507579	5.8511835261649505
>100	0.042647560559535995	2.882921133637618
>500	0.002437003460544914	0.5515338074514382
>1k	0.0085295121119072	7.905317906803949
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGT	4264	1.810423521919117	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	4218	1.7908926865513215	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCC	4125	1.7514064324381702	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2001	0.8495913384990977	No Hit
CTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGCT	1941	0.8241163358454517	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTT	1052	0.4466617131939285	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT	1018	0.4322258783568623	No Hit
AACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG	693	0.29423628064961255	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTC	606	0.2572975268018257	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTAT	489	0.2076212716272158	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	477	0.20252627109648658	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	434	0.18426918586137353	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	433	0.18384460248381276	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTT	336	0.1426600148604182	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	296	0.1256766797579875	No Hit
AATCTGACTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	257	0.1091179280331175	No Hit
AATCTGTCACTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	244	0.10359834412482752	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0584863602589958	0.0
2	0.0	0.0	0.0	1.2992251353359516	0.0
3	0.0	0.0	0.0	1.9263347839932066	0.0
4	0.0	0.0	0.0	5.23214096168135	0.0
5	0.0	0.0	0.0	5.775183101581573	0.0
6	0.0	0.0	0.0	7.338923681137883	0.0
7	0.0	0.0	0.0	8.66319923574992	0.0
8	0.0	0.0	0.0	9.212185542935995	0.0
9	0.0	0.0	0.0	12.064112090011676	0.0
10	0.0	0.0	0.0	13.312811803417896	0.0
11	0.0	0.0	0.0	16.534550472349007	0.0
12	0.0	0.0	0.0	17.3247001379896	0.0
13	0.0	0.0	0.0	17.805328521388386	0.0
14	0.0	0.0	0.0	18.346672327778368	0.0
15	0.0	0.0	0.0	18.665959027704066	0.0
16	0.0	0.0	0.0	19.16739199660333	0.0
17	0.0	0.0	0.0	19.69599830166649	0.0
18	0.0	0.0	0.0	20.536248805859252	0.0
19	0.0	0.0	0.0	20.81859675193716	0.0
20	0.0	0.0	0.0	21.17185012206772	0.0
21	4.245833775607685E-4	0.0	0.0	21.515762657891944	0.0
22	4.245833775607685E-4	0.0	0.0	21.883027279482008	0.0
23	4.245833775607685E-4	0.0	0.0	22.241375650143297	0.0
24	4.245833775607685E-4	0.0	0.0	22.502069843965607	0.0
25	4.245833775607685E-4	0.0	0.0	22.749177369705976	0.0
26	4.245833775607685E-4	0.0	0.0	22.954675724445387	0.0
27	4.245833775607685E-4	0.0	0.0	23.21919116866575	0.0
28	4.245833775607685E-4	0.0	0.0	23.457807026854898	0.0
29	4.245833775607685E-4	0.0	0.0	23.696847468421613	0.0
30	4.245833775607685E-4	0.0	0.0	24.06114000636875	0.0
31	4.245833775607685E-4	0.0	0.0	24.31461628277253	0.0
32	4.245833775607685E-4	0.0	0.0	24.579131726992888	0.0
33	4.245833775607685E-4	0.0	0.0	24.825814669355694	0.0
34	4.245833775607685E-4	0.0	0.0	25.051693026218025	0.0
35	4.245833775607685E-4	0.0	0.0	25.324275554612036	0.0
36	4.245833775607685E-4	0.0	0.0	25.573081413862646	0.0
37	4.245833775607685E-4	0.0	0.0	25.79428935357181	0.0
38	4.245833775607685E-4	0.0	0.0	26.04521812971022	0.0
39	4.245833775607685E-4	0.0	0.0	26.30930899055302	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	20	7.021853E-4	45.000004	1
ACACGAC	20	7.021853E-4	45.000004	26
ACTGCCG	30	2.158129E-6	45.000004	1
CTCGTGT	20	7.021853E-4	45.000004	30
CGTATGG	20	7.021853E-4	45.000004	2
CGACGTT	35	1.2064265E-7	45.000004	27
GGCCGGT	20	7.021853E-4	45.000004	9
ATTCGGG	20	7.021853E-4	45.000004	3
ATGCTAG	30	2.158129E-6	45.000004	1
ATTCGAC	20	7.021853E-4	45.000004	21
GTCTACT	20	7.021853E-4	45.000004	16
ACTTAGC	20	7.021853E-4	45.000004	36
TAGCATA	20	7.021853E-4	45.000004	30
CCCGTGA	20	7.021853E-4	45.000004	25
GTTAATC	20	7.021853E-4	45.000004	40
TACGAAG	20	7.021853E-4	45.000004	1
ACCAGAC	20	7.021853E-4	45.000004	42
GACGGTT	20	7.021853E-4	45.000004	25
GACGGCT	20	7.021853E-4	45.000004	23
CGTAATG	20	7.021853E-4	45.000004	1
>>END_MODULE