##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548642_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	192336
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.979842567174114	31.0	31.0	33.0	28.0	34.0
2	31.148198985109392	31.0	31.0	34.0	28.0	34.0
3	31.372301597204892	31.0	31.0	34.0	28.0	34.0
4	35.34696572664504	37.0	35.0	37.0	33.0	37.0
5	34.94334913900674	35.0	35.0	37.0	32.0	37.0
6	34.940843107894516	35.0	35.0	37.0	32.0	37.0
7	33.756883786706595	35.0	35.0	37.0	30.0	37.0
8	34.55273063805008	37.0	35.0	37.0	31.0	37.0
9	35.88607956908743	38.0	35.0	39.0	32.0	39.0
10	35.67089884369021	37.0	35.0	39.0	30.0	39.0
11	36.07816009483404	37.0	35.0	39.0	31.0	39.0
12	35.716667706513604	37.0	35.0	39.0	31.0	39.0
13	35.52149883537143	37.0	35.0	39.0	30.0	39.0
14	36.48939356126778	38.0	35.0	40.0	31.0	41.0
15	36.6339166874636	38.0	35.0	40.0	31.0	41.0
16	36.57359516679145	38.0	35.0	40.0	31.0	41.0
17	36.46025705016222	38.0	35.0	40.0	31.0	41.0
18	36.252958364528745	38.0	35.0	40.0	30.0	41.0
19	36.22805922968139	38.0	34.0	40.0	30.0	41.0
20	36.261448714749186	38.0	34.0	40.0	30.0	41.0
21	36.35250291157141	38.0	35.0	40.0	31.0	41.0
22	36.336073330005824	38.0	35.0	40.0	30.0	41.0
23	36.31433013060477	38.0	35.0	40.0	31.0	41.0
24	36.071437484402296	38.0	34.0	40.0	30.0	41.0
25	36.06134576990267	38.0	34.0	40.0	30.0	41.0
26	35.93117253140338	38.0	34.0	40.0	30.0	41.0
27	35.816160261209546	38.0	34.0	40.0	30.0	41.0
28	35.6592265618501	38.0	34.0	40.0	29.0	41.0
29	35.44771649613177	38.0	34.0	40.0	29.0	41.0
30	35.491530446718244	38.0	34.0	40.0	29.0	41.0
31	35.276022169536645	37.0	34.0	40.0	27.0	41.0
32	35.228553156975295	37.0	34.0	40.0	27.0	41.0
33	35.1757861242825	37.0	34.0	40.0	27.0	41.0
34	35.030410323600364	37.0	34.0	40.0	26.0	41.0
35	35.10313721820148	38.0	34.0	40.0	27.0	41.0
36	34.86517864570335	37.0	33.0	40.0	26.0	41.0
37	34.8915803593711	37.0	34.0	40.0	26.0	41.0
38	34.74755116046918	37.0	33.0	40.0	26.0	41.0
39	34.60948548373679	37.0	33.0	40.0	25.0	41.0
40	34.48065364778304	37.0	33.0	40.0	24.0	41.0
41	34.41358871974045	37.0	33.0	40.0	24.0	41.0
42	34.34675775725813	37.0	33.0	40.0	24.0	41.0
43	34.226982988104155	37.0	33.0	40.0	23.0	41.0
44	34.18703206887946	37.0	33.0	40.0	23.0	41.0
45	33.89034814075368	36.0	33.0	40.0	23.0	41.0
46	33.70861929124033	36.0	32.0	39.0	23.0	41.0
47	33.60053240163048	36.0	32.0	39.0	23.0	40.0
48	33.51080920888445	35.0	32.0	39.0	22.0	40.0
49	33.391164420597285	35.0	32.0	39.0	22.0	40.0
50	33.26288890275352	35.0	32.0	39.0	21.0	40.0
51	30.989617128358706	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	8.0
11	6.0
12	4.0
13	12.0
14	12.0
15	29.0
16	45.0
17	75.0
18	156.0
19	333.0
20	526.0
21	857.0
22	1187.0
23	1591.0
24	1887.0
25	2201.0
26	2561.0
27	3087.0
28	3769.0
29	4547.0
30	5788.0
31	7581.0
32	9709.0
33	13087.0
34	20414.0
35	19905.0
36	19925.0
37	25792.0
38	30576.0
39	16663.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.75397221528991	39.43099575742451	17.94411862573829	22.87091340154729
2	22.43001830130605	37.65129772897429	18.882060560685467	21.036623409034192
3	20.916001164628568	30.48675235005407	22.91874636053573	25.678500124781635
4	23.321687047666583	30.279822810082358	18.582064720073205	27.816425422177854
5	16.807046002828383	36.83813742617087	18.3538183179436	28.00099825305715
6	19.940624740038267	36.82877880376009	22.607312203643623	20.623284252558026
7	74.03554196822228	13.436902088012644	7.242533899010066	5.285022044755012
8	78.0363530488312	7.287767240662174	5.7373554612761	8.938524249230513
9	72.9010689626487	11.352008984277514	9.791718659013393	5.955203394060395
10	39.29165626819732	31.47304716745695	13.61419599035022	15.621100573995509
11	34.22448215622661	27.222152899093256	22.527243989684717	16.026120954995424
12	30.95000415938774	23.541094750852675	29.571167124199317	15.93773396556027
13	25.831877547624988	30.979119873554612	21.925692538058396	21.263310040761997
14	17.08520505781549	34.250998253057155	30.43112053905665	18.23267615007071
15	18.515514516263206	29.419349471757755	30.890212960652192	21.174923051326843
16	20.291053156975295	28.781403377422844	30.329215539472585	20.598327926129272
17	17.867689876050246	28.960776973629482	27.353173612844188	25.81835953747608
18	24.07037684052907	28.347787205723318	24.05217951917478	23.529656434572832
19	22.648386157557606	33.29745861409201	25.50692538058398	18.54722984776641
20	26.980908410282005	30.410323600366024	22.90938773812495	19.69938025122702
21	21.366254887280594	31.65346061059812	24.5372681141336	22.443016387987686
22	20.78758006821396	29.702707761417518	26.729265452125446	22.780446718243073
23	23.627402046418766	28.769445137675735	22.491889193910655	25.111263621994844
24	20.774581981532318	30.70823974710922	25.921824307461943	22.595353963896514
25	20.028491806006155	28.28123700191332	25.37746443723484	26.312806754845685
26	20.93835787372099	32.314283337492725	23.28997171616338	23.45738707262291
27	17.964915564428914	30.470114799101573	26.373117877048497	25.191851759421013
28	22.600033275101904	31.114299975043675	26.534294151900838	19.75137259795358
29	21.51235338158223	33.11288578321271	25.80588137426171	19.56887946094335
30	22.821520672157057	27.31729889360286	27.646930371849265	22.214250062390818
31	27.4452000665502	33.42639963397388	20.375800682139587	18.752599617336326
32	24.704163547125862	27.45143914815739	25.43673571250312	22.407661592213625
33	25.228766325596872	28.90878462690292	23.05132684468846	22.811122202811745
34	21.838345395557774	29.01900840196323	25.89478828716413	23.247857915314864
35	20.922240246235756	28.997171616338075	26.134992929040845	23.945595208385324
36	23.77402046418767	31.88014724232593	24.970884285833126	19.374948007653273
37	22.430538224773315	29.314844854837368	24.766034439730472	23.48858248065885
38	21.94804924715082	31.906143415689208	24.26794775809001	21.877859579069963
39	23.85616837201564	25.503285916313118	26.954912236918727	23.685633474752514
40	24.487875384743365	26.463064636885452	22.437297229847765	26.611762748523415
41	21.15412611263622	25.043673571250313	28.544318276349724	25.257882039763746
42	25.33587055985359	24.857021046501952	23.367960236253225	26.439148157391234
43	21.499875218367855	26.398594126944513	25.011438316279843	27.090092338407786
44	21.722922385824806	24.850781964894768	25.640025788203975	27.786269861076452
45	25.738811246984444	29.133911488228932	22.928624906413773	22.198652358372847
46	20.958634888944346	26.36479910157225	30.030259545794856	22.646306463688546
47	21.71772315115215	27.975522003161135	23.97003161134681	26.336723234339903
48	22.994135263289245	22.883391564761666	32.29036685799851	21.832106313950586
49	22.713896514433078	22.119104067881207	28.90878462690292	26.258214790782798
50	20.336286498627402	25.45181349305382	26.818692288495132	27.393207719823643
51	19.105107728142418	24.012145412195324	31.43665252474836	25.446094334913898
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1934.0
1	1531.0
2	1128.0
3	755.0
4	382.0
5	355.5
6	329.0
7	333.0
8	337.0
9	367.0
10	397.0
11	435.5
12	474.0
13	475.0
14	476.0
15	481.5
16	487.0
17	527.5
18	568.0
19	573.5
20	579.0
21	600.5
22	622.0
23	650.5
24	679.0
25	693.5
26	1082.0
27	1456.0
28	1613.5
29	1771.0
30	2342.5
31	2914.0
32	3137.0
33	3360.0
34	3918.0
35	4476.0
36	4717.0
37	4958.0
38	5385.0
39	5812.0
40	6268.0
41	6724.0
42	8992.0
43	11260.0
44	15824.5
45	20389.0
46	20561.0
47	20733.0
48	19459.5
49	18186.0
50	17025.5
51	15865.0
52	14723.5
53	13582.0
54	12189.0
55	10796.0
56	9730.0
57	8664.0
58	8323.5
59	7983.0
60	7289.0
61	6595.0
62	6016.5
63	5438.0
64	4588.5
65	3739.0
66	3120.5
67	2502.0
68	2212.0
69	1922.0
70	1753.0
71	1584.0
72	1307.0
73	1030.0
74	838.0
75	482.0
76	318.0
77	277.0
78	236.0
79	179.5
80	123.0
81	88.5
82	54.0
83	47.0
84	40.0
85	31.0
86	22.0
87	34.5
88	47.0
89	26.5
90	6.0
91	5.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	192336.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.250436735712505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.63738380370154	29.665273271774396
2	7.442146107249532	5.544463854920555
3	2.569578203947185	2.8715373097080112
4	1.3831895709460402	2.060976624240912
5	0.9198001284091227	1.713147824640213
6	0.8276805404349161	1.8498876965310707
7	0.6029645758311699	1.5722485650112301
8	0.576445300505262	1.7178271358456034
9	0.47595120453340034	1.5956451210381832
>10	5.383412891159311	40.37933616171699
>50	0.14236663596013735	3.2801971549787874
>100	0.030706529324735502	2.730118126611763
>500	0.001395751332942523	0.3821437484402296
>1k	0.006978756664712616	4.637197404542051
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCC	2068	1.075201730305299	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGT	2032	1.0564844854837367	No Hit
AATCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCT	2010	1.0450461692038933	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1641	0.8531944097828801	No Hit
CTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGCT	1168	0.6072706097662424	Illumina Single End Adapter 1 (95% over 22bp)
AACCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCGATATCGCTC	735	0.3821437484402296	No Hit
AATGACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTT	480	0.24956326428749687	No Hit
AACCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCGATATCGC	429	0.22304716745695036	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCT	417	0.2168080858497629	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	408	0.21212877464437235	No Hit
AAACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCT	369	0.19185175942101323	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	335	0.1741743615339822	No Hit
AACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTG	332	0.17261459113218533	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTT	276	0.1434988769653107	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGATATCGCTCGTAT	247	0.12842109641460778	No Hit
AATGCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTC	237	0.12322186174195159	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	215	0.11178354546210797	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	200	0.1039846934531237	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8391564761667083	0.0
2	0.0	0.0	0.0	1.0486856334747525	0.0
3	0.0	0.0	0.0	1.5322144580317778	0.0
4	0.0	0.0	0.0	4.186943681890026	0.0
5	0.0	0.0	0.0	4.589364445553614	0.0
6	0.0	0.0	0.0	6.167852092172033	0.0
7	0.0	0.0	0.0	7.2992055569420184	0.0
8	0.0	0.0	0.0	7.805091090591465	0.0
9	0.0	0.0	0.0	9.92637883703519	0.0
10	0.0	0.0	0.0	11.181994010481658	0.0
11	0.0	0.0	0.0	13.701543132850844	0.0
12	0.0	0.0	0.0	14.525101904999584	0.0
13	0.0	0.0	0.0	14.898406954496298	0.0
14	0.0	0.0	0.0	15.407931952416604	0.0
15	0.0	0.0	0.0	15.69908909408535	0.0
16	0.0	0.0	0.0	16.336515264953	0.0
17	0.0	0.0	0.0	16.846040262873306	0.0
18	0.0	0.0	0.0	18.070460028283836	0.0
19	0.0	0.0	0.0	18.374615256634222	0.0
20	0.0	0.0	0.0	19.009441810165544	0.0
21	0.0	0.0	0.0	19.39314532900757	0.0
22	0.0	0.0	0.0	19.784127776391315	0.0
23	0.0	0.0	0.0	20.18706846352217	0.0
24	0.0	0.0	0.0	20.471466600116464	0.0
25	0.0	0.0	0.0	20.732468180683803	0.0
26	0.0	0.0	0.0	20.968513434822395	0.0
27	0.0	0.0	0.0	21.260190499958405	0.0
28	0.0	0.0	0.0	21.51807253972215	0.0
29	0.0	0.0	0.0	21.759836952000665	0.0
30	0.0	0.0	0.0	22.157058480991598	0.0
31	0.0	0.0	0.0	22.40974128608269	0.0
32	0.0	0.0	0.0	22.725854754180183	0.0
33	0.0	0.0	0.0	23.00401380916729	0.0
34	0.0	0.0	0.0	23.244218451044006	0.0
35	0.0	0.0	0.0	23.50937941934947	0.0
36	0.0	0.0	0.0	23.75062390816072	0.0
37	0.0	0.0	0.0	23.999147325513686	0.0
38	0.0	0.0	0.0	24.309021712003993	0.0
39	0.0	0.0	0.0	24.603298394476333	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGGT	55	1.8189894E-12	45.000004	9
TAGAGGT	20	7.018626E-4	45.0	29
CGGAAAT	20	7.018626E-4	45.0	14
CCGGGAC	20	7.018626E-4	45.0	5
ATTAGAC	20	7.018626E-4	45.0	30
CGCGGCT	20	7.018626E-4	45.0	36
CTATCCG	20	7.018626E-4	45.0	1
TATGGGA	25	3.8786588E-5	45.0	4
TTAGACG	20	7.018626E-4	45.0	31
TTATGGG	35	1.2048804E-7	45.0	3
TGGAAGT	20	7.018626E-4	45.0	15
ATTACGG	20	7.018626E-4	45.0	2
GACCGAT	25	3.8786588E-5	45.0	9
CGACAAT	20	7.018626E-4	45.0	20
CTAACGG	35	1.2048804E-7	45.0	2
CTAACCG	20	7.018626E-4	45.0	1
TCGGAAA	20	7.018626E-4	45.0	13
CGTAGGG	70	0.0	45.0	3
CGGGCAA	35	1.2048804E-7	45.0	6
ACTACGG	40	6.7648216E-9	45.0	2
>>END_MODULE