SRR3548638_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548638_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	182919
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGT	2821	1.5422126733690869	No Hit
AATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	2752	1.5044910588839868	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC	2684	1.4673161344638883	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2165	1.1835839907281365	No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	1997	1.091740059807893	RNA PCR Primer, Index 27 (96% over 25bp)
AATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT	695	0.37994959517600685	No Hit
AAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	615	0.3362143899758909	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	549	0.30013284568579535	No Hit
AACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTG	539	0.29466594503578086	RNA PCR Primer, Index 27 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	528	0.2886523543207649	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT	452	0.24710390938065485	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	444	0.24273038886064324	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTAT	308	0.1683805400204462	No Hit
AATGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	289	0.15799342878541867	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	249	0.13612582618536073	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	238	0.1301122354703448	No Hit
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	217	0.11863174410531438	RNA PCR Primer, Index 27 (95% over 24bp)
AAGGTCGGGCGATGAGGCAGGGTACAGGAGGATCACTTGAGGCTAGGAGTT	204	0.11152477326029556	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	199	0.1087913229352883	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTGTA	20	7.0177205E-4	45.000004	8
CGGGTAC	20	7.0177205E-4	45.000004	6
TCGTTCT	20	7.0177205E-4	45.000004	10
ATCTATG	20	7.0177205E-4	45.000004	1
AAATACG	20	7.0177205E-4	45.000004	1
TGGGTCG	20	7.0177205E-4	45.000004	6
CGGCAGG	20	7.0177205E-4	45.000004	2
TAAACGG	30	2.155466E-6	45.000004	2
TGAACGG	20	7.0177205E-4	45.000004	2
AATGGTC	40	6.7611836E-9	45.000004	9
TTCAACG	20	7.0177205E-4	45.000004	1
ATATGCT	20	7.0177205E-4	45.000004	34
CGTTCTT	20	7.0177205E-4	45.000004	11
GCCGATG	20	7.0177205E-4	45.000004	9
GTAGCAT	20	7.0177205E-4	45.000004	29
TACGCGG	20	7.0177205E-4	45.000004	2
GTCGTTC	20	7.0177205E-4	45.000004	9
GTACTGG	30	2.155466E-6	45.000004	2
CGGGACG	20	7.0177205E-4	45.000004	6
AACTGCT	20	7.0177205E-4	45.000004	20

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content