##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548638_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	182919
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.74334541518377	31.0	30.0	33.0	28.0	34.0
2	30.912021167839317	31.0	31.0	34.0	28.0	34.0
3	31.15790049147437	31.0	31.0	34.0	28.0	34.0
4	35.164395169446585	35.0	35.0	37.0	33.0	37.0
5	34.70496777262067	35.0	35.0	37.0	32.0	37.0
6	34.73353779541765	35.0	35.0	37.0	32.0	37.0
7	33.572728912797466	35.0	35.0	37.0	30.0	37.0
8	34.338871303691796	35.0	35.0	37.0	30.0	37.0
9	35.74007620859506	38.0	35.0	39.0	31.0	39.0
10	35.363226346087615	37.0	35.0	39.0	30.0	39.0
11	35.8478452211088	37.0	35.0	39.0	31.0	39.0
12	35.57525462089777	37.0	35.0	39.0	30.0	39.0
13	35.353642869248134	37.0	35.0	39.0	30.0	39.0
14	36.32265100946321	38.0	35.0	40.0	30.0	41.0
15	36.503971703322236	38.0	35.0	40.0	31.0	41.0
16	36.424778180506124	38.0	35.0	40.0	31.0	41.0
17	36.37869767492715	38.0	35.0	40.0	31.0	41.0
18	36.10695991121754	38.0	35.0	40.0	30.0	41.0
19	36.06453676217342	38.0	34.0	40.0	30.0	41.0
20	36.13002476505994	38.0	34.0	40.0	30.0	41.0
21	36.27066625118222	38.0	34.0	40.0	31.0	41.0
22	36.23301570640557	38.0	34.0	40.0	30.0	41.0
23	36.19830088727797	38.0	35.0	40.0	30.0	41.0
24	35.87442529206917	38.0	34.0	40.0	30.0	41.0
25	35.956898955275285	38.0	34.0	40.0	30.0	41.0
26	35.80760883232469	38.0	34.0	40.0	30.0	41.0
27	35.71791886026055	38.0	34.0	40.0	29.0	41.0
28	35.66366533820981	38.0	34.0	40.0	29.0	41.0
29	35.526965487456195	38.0	34.0	40.0	29.0	41.0
30	35.49700140499347	38.0	34.0	40.0	29.0	41.0
31	35.33226182080593	38.0	34.0	40.0	28.0	41.0
32	35.039963043751605	37.0	34.0	40.0	27.0	41.0
33	34.98338062202396	37.0	34.0	40.0	26.0	41.0
34	35.008648636828326	37.0	34.0	40.0	27.0	41.0
35	34.986627961010065	37.0	34.0	40.0	26.0	41.0
36	34.92080101028324	37.0	34.0	40.0	26.0	41.0
37	34.70307622499576	37.0	33.0	40.0	25.0	41.0
38	34.557837075426825	37.0	33.0	40.0	24.0	41.0
39	34.31073863294682	37.0	33.0	40.0	24.0	41.0
40	34.03054357393163	37.0	33.0	40.0	23.0	41.0
41	34.284049223973454	37.0	33.0	40.0	23.0	41.0
42	34.20303522324089	37.0	33.0	40.0	23.0	41.0
43	34.18150110158048	37.0	33.0	40.0	23.0	41.0
44	34.09146671477539	37.0	33.0	40.0	23.0	41.0
45	33.74676769499068	37.0	33.0	40.0	23.0	40.0
46	33.657826688315595	36.0	32.0	39.0	22.0	40.0
47	33.49977312362302	36.0	32.0	39.0	22.0	40.0
48	33.42253128433897	36.0	32.0	39.0	21.0	40.0
49	33.28367747472925	36.0	32.0	39.0	20.0	40.0
50	33.10542917903553	36.0	32.0	39.0	19.0	40.0
51	30.84964383142265	34.0	28.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	4.0
11	2.0
12	3.0
13	4.0
14	7.0
15	11.0
16	26.0
17	94.0
18	180.0
19	339.0
20	519.0
21	915.0
22	1188.0
23	1596.0
24	1900.0
25	2201.0
26	2609.0
27	3007.0
28	3731.0
29	4553.0
30	5803.0
31	7369.0
32	9631.0
33	12650.0
34	18587.0
35	19638.0
36	20129.0
37	25426.0
38	27892.0
39	12904.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.029838343747777	40.295978001191784	19.484580606716634	22.189603048343802
2	18.51311236120906	39.846052077695596	20.273454370513726	21.367381190581625
3	19.01169370049038	33.41861698347356	25.37625943723725	22.193429878798813
4	22.67178368567508	33.08677611401768	19.26754465091106	24.97389554939618
5	15.512877284481108	40.30417835216681	20.18051705946348	24.002427303888606
6	19.737698106812303	36.16081434952083	23.579835883642485	20.521651660024382
7	68.51447908637157	15.933828634532224	9.126443945134186	6.425248333962028
8	73.21328019505901	8.360531164067156	6.464610018642131	11.961578622231698
9	68.3433650960261	12.607219588998408	12.212509362067363	6.8369059529081175
10	33.022813376412515	37.376106364019044	13.854766317331716	15.746313942236728
11	27.196190663627068	24.3402817640595	30.70922102132638	17.75430655098705
12	27.009222661396574	23.971812660248524	32.59366167538637	16.425303002968526
13	22.01083539708833	34.60110759407169	24.598319474740187	18.789737534099793
14	13.933489686691924	35.34733953279867	33.50499401374379	17.21417676676562
15	13.334317375450336	32.84349903509203	31.269031647887864	22.553151941569766
16	15.016482705459794	30.4140083862256	33.63346617901913	20.936042729295483
17	15.1318343091751	31.13399920183251	27.525844772822943	26.208321716169454
18	17.771800633067095	30.406354725315577	26.58444448089045	25.23740016072688
19	18.75037584941969	35.391621428063786	28.062694416654367	17.795308305862157
20	21.167839316856092	33.98225444049005	25.40796746100733	19.44193878164652
21	16.688807614299225	33.543262318293884	25.15211651058665	24.615813556820232
22	16.84898780334465	33.03320048764754	27.333409869942432	22.784401839065378
23	19.963481103657905	31.926153106019605	21.31708570460149	26.793280085721
24	14.714163099513994	36.43798621247656	26.012606672898936	22.835244015110515
25	17.76688042248208	30.68790010879132	26.292511986179672	25.25270748254692
26	19.61851967264199	36.226417157321	21.548882292162105	22.606180877874905
27	14.734937321984049	32.76860249618684	26.40403675943997	26.092423422389142
28	22.026689408973372	28.73129636615114	26.23073600883451	23.01127821604098
29	16.88288258737474	29.80280889355398	27.40393288832762	25.910375630743665
30	20.71025973244988	30.14011666365987	22.534017789294715	26.615605814595533
31	23.904023092188346	30.024218369879563	19.757378949152358	26.314379588779737
32	20.540239122234432	28.25731607979488	23.923157244463397	27.27928755350729
33	23.25182184464162	30.96179183135705	21.08364904684587	24.702737277155464
34	16.63031177734407	26.43793154347006	27.068265188416728	29.86349149076914
35	19.028094402440423	27.196190663627068	27.916728169298977	25.858986764633524
36	19.246770428441003	25.635390528047935	29.22933101536746	25.888508028143605
37	16.496372711418715	27.65158348777328	27.981784287034152	27.870259513773853
38	19.306906335591165	29.30204079401265	28.251849179144866	23.13920369125132
39	16.281523515873143	27.687118341998374	34.407579311061184	21.623778831067302
40	20.518918209699375	30.28444284082025	26.50681449166024	22.68982445782013
41	17.263378872615746	23.255101985031626	35.568749009124254	23.91277013322837
42	23.696827557552798	24.50210202329993	27.277647483312283	24.523422935834986
43	19.48786074710664	24.334268173344487	28.95106577228172	27.22680530726715
44	19.78854028285744	24.84870352451085	26.734237558700848	28.628518633930867
45	23.999147163498595	29.19980975185738	24.231490441124212	22.569552643519806
46	18.333798019888583	25.561587369272736	33.41096332256354	22.693651288275138
47	20.446208431054185	26.676835101875696	25.477397099262518	27.39955936780761
48	19.719110644602257	22.541124760139734	35.50205282119408	22.237711774063932
49	20.706979592059874	21.133397842761003	30.755689676851507	27.40393288832762
50	19.262077750261046	23.711041499242835	26.66590130057567	30.360979449920457
51	16.70958183676928	24.504288783559936	33.00695936452747	25.779170015143315
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2521.0
1	2039.5
2	1558.0
3	1058.5
4	559.0
5	510.5
6	462.0
7	491.5
8	521.0
9	587.0
10	653.0
11	721.5
12	790.0
13	808.5
14	827.0
15	824.5
16	822.0
17	784.5
18	747.0
19	697.0
20	647.0
21	640.0
22	633.0
23	675.0
24	717.0
25	762.5
26	950.0
27	1092.0
28	1272.5
29	1453.0
30	1859.0
31	2265.0
32	2506.5
33	2748.0
34	3263.0
35	3778.0
36	4363.0
37	4948.0
38	5297.0
39	5646.0
40	6644.0
41	7642.0
42	10490.0
43	13338.0
44	18967.0
45	24596.0
46	24384.5
47	24173.0
48	22928.0
49	21683.0
50	19389.5
51	17096.0
52	14875.0
53	12654.0
54	10532.5
55	8411.0
56	7272.5
57	6134.0
58	5168.0
59	4202.0
60	3638.0
61	3074.0
62	2502.0
63	1930.0
64	1639.5
65	1349.0
66	1100.0
67	851.0
68	644.0
69	437.0
70	413.5
71	390.0
72	354.0
73	318.0
74	280.5
75	169.0
76	95.0
77	70.0
78	45.0
79	32.0
80	19.0
81	20.5
82	22.0
83	16.0
84	10.0
85	7.5
86	5.0
87	3.0
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	182919.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.84170589167883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.35911440553984	36.482814797806675
2	8.296352287135472	7.440451784669717
3	2.7260314054423094	3.667196956029718
4	1.4544523554081732	2.6088049901869135
5	0.9168048376085048	2.055554644405447
6	0.6656588315614942	1.790956652944746
7	0.4791280600799766	1.5039443688189855
8	0.412074514776163	1.4782499357639174
9	0.38037647517799666	1.535105702524068
>10	3.1929679118306837	28.263876360574898
>50	0.074368477518775	2.1894937103308023
>100	0.030478884229006145	2.5945910484968757
>500	0.006095776845801229	1.5996151301942392
>1k	0.006095776845801229	6.789343917252992
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGT	2821	1.5422126733690869	No Hit
AATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	2752	1.5044910588839868	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC	2684	1.4673161344638883	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2165	1.1835839907281365	No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	1997	1.091740059807893	RNA PCR Primer, Index 27 (96% over 25bp)
AATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT	695	0.37994959517600685	No Hit
AAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	615	0.3362143899758909	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	549	0.30013284568579535	No Hit
AACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTG	539	0.29466594503578086	RNA PCR Primer, Index 27 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	528	0.2886523543207649	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT	452	0.24710390938065485	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	444	0.24273038886064324	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTAT	308	0.1683805400204462	No Hit
AATGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	289	0.15799342878541867	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	249	0.13612582618536073	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	238	0.1301122354703448	No Hit
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	217	0.11863174410531438	RNA PCR Primer, Index 27 (95% over 24bp)
AAGGTCGGGCGATGAGGCAGGGTACAGGAGGATCACTTGAGGCTAGGAGTT	204	0.11152477326029556	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	199	0.1087913229352883	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4345147305638015	0.0
2	0.0	0.0	0.0	1.793143413204752	0.0
3	0.0	0.0	0.0	2.6334060431119783	0.0
4	0.0	0.0	0.0	6.765289554392928	0.0
5	0.0	0.0	0.0	7.3562615146594945	0.0
6	0.0	0.0	0.0	9.796685964825961	0.0
7	0.0	0.0	0.0	11.78062421071622	0.0
8	0.0	0.0	0.0	12.923753136634248	0.0
9	0.0	0.0	0.0	16.321431890618253	0.0
10	0.0	0.0	0.0	18.51365905127406	0.0
11	0.0	0.0	0.0	22.436706957724457	0.0
12	0.0	0.0	0.0	23.79249831892805	0.0
13	0.0	0.0	0.0	24.415724993029702	0.0
14	0.0	0.0	0.0	25.11658165636156	0.0
15	0.0	0.0	0.0	25.54409328719269	0.0
16	0.0	0.0	0.0	26.281578184879645	0.0
17	0.0	0.0	0.0	27.194003903367065	0.0
18	0.0	0.0	0.0	28.420229719165313	0.0
19	0.0	0.0	0.0	28.9434121113717	0.0
20	0.0	0.0	0.0	29.48354189559313	0.0
21	0.0	0.0	0.0	30.097474838589758	0.0
22	0.0	0.0	0.0	30.741475735161465	0.0
23	0.0	0.0	0.0	31.419918105828263	0.0
24	0.0	0.0	0.0	31.894445082249522	0.0
25	0.0	0.0	0.0	32.33453058457569	0.0
26	0.0	0.0	0.0	32.75766869488681	0.0
27	0.0	0.0	0.0	33.15893920259787	0.0
28	0.0	0.0	0.0	33.58809090362401	0.0
29	0.0	0.0	0.0	34.0380168271202	0.0
30	0.0	0.0	0.0	34.65522991050683	0.0
31	0.0	0.0	0.0	35.095315412833	0.0
32	0.0	0.0	0.0	35.48838556956905	0.0
33	0.0	0.0	0.0	35.8863759368901	0.0
34	0.0	0.0	0.0	36.23789764868603	0.0
35	0.0	0.0	0.0	36.722265046277315	0.0
36	0.0	0.0	0.0	37.089094079893286	0.0
37	0.0	0.0	0.0	37.44881614266424	0.0
38	0.0	0.0	0.0	37.82603228751524	0.0
39	0.0	0.0	0.0	38.2251160349663	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTGTA	20	7.0177205E-4	45.000004	8
CGGGTAC	20	7.0177205E-4	45.000004	6
TCGTTCT	20	7.0177205E-4	45.000004	10
ATCTATG	20	7.0177205E-4	45.000004	1
AAATACG	20	7.0177205E-4	45.000004	1
TGGGTCG	20	7.0177205E-4	45.000004	6
CGGCAGG	20	7.0177205E-4	45.000004	2
TAAACGG	30	2.155466E-6	45.000004	2
TGAACGG	20	7.0177205E-4	45.000004	2
AATGGTC	40	6.7611836E-9	45.000004	9
TTCAACG	20	7.0177205E-4	45.000004	1
ATATGCT	20	7.0177205E-4	45.000004	34
CGTTCTT	20	7.0177205E-4	45.000004	11
GCCGATG	20	7.0177205E-4	45.000004	9
GTAGCAT	20	7.0177205E-4	45.000004	29
TACGCGG	20	7.0177205E-4	45.000004	2
GTCGTTC	20	7.0177205E-4	45.000004	9
GTACTGG	30	2.155466E-6	45.000004	2
CGGGACG	20	7.0177205E-4	45.000004	6
AACTGCT	20	7.0177205E-4	45.000004	20
>>END_MODULE