FastQCFastQC Report
Sat 18 Jun 2016
SRR3548634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251303
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT15650.6227542050831069Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14500.5769927139747636No Hit
AATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT7750.3083926574692702No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCC7640.30401547136325474No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGT6260.24910168203324273No Hit
AAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT3450.13728447332502994No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3280.13051973116118787No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3160.1257446190455347No Hit
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC2660.10584831856364628No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG2570.10226698447690637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGG900.045.0000043
ACGTGGG453.8198777E-1045.0000043
TTAACGG650.045.0000042
GTTTGGC207.0227584E-445.026
ACCGGGT207.0227584E-445.04
TAGACGG406.7793735E-945.02
TAGCGTT207.0227584E-445.015
CGTTGAA207.0227584E-445.018
AGCCACG253.8820803E-545.01
ATTAACG253.8820803E-545.01
CGCTGCG207.0227584E-445.01
GCGTTGA207.0227584E-445.017
GCGCAAA207.0227584E-445.039
AGAACGG207.0227584E-445.02
ACGATGT207.0227584E-445.027
TACGCAG207.0227584E-445.01
GTTCGGC253.8820803E-545.031
TCAGCCG207.0227584E-445.029
ATCCACC351.2068631E-745.025
CGCAGTT207.0227584E-445.03