##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548634_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	251303
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.471434881398153	31.0	30.0	33.0	27.0	34.0
2	30.758729501836427	31.0	31.0	34.0	27.0	34.0
3	31.10558966665738	31.0	31.0	34.0	28.0	34.0
4	35.20848139496942	37.0	35.0	37.0	33.0	37.0
5	34.88599817749888	35.0	35.0	37.0	32.0	37.0
6	34.985973108160266	35.0	35.0	37.0	32.0	37.0
7	33.98882225838928	36.0	35.0	37.0	31.0	37.0
8	34.71360071308341	37.0	35.0	37.0	32.0	37.0
9	35.94747376672782	39.0	35.0	39.0	32.0	39.0
10	35.610716943291564	37.0	35.0	39.0	30.0	39.0
11	36.073596415482506	37.0	35.0	39.0	31.0	39.0
12	35.96146484522668	37.0	35.0	39.0	31.0	39.0
13	35.729386437885736	37.0	35.0	39.0	30.0	39.0
14	36.78839488585493	38.0	35.0	40.0	31.0	41.0
15	36.89856070162314	38.0	35.0	40.0	32.0	41.0
16	36.91966271791423	38.0	35.0	40.0	32.0	41.0
17	36.89874772684767	38.0	35.0	40.0	32.0	41.0
18	36.73163869910029	38.0	35.0	40.0	31.0	41.0
19	36.74990748220276	38.0	35.0	40.0	31.0	41.0
20	36.701654974274085	38.0	35.0	40.0	31.0	41.0
21	36.73666450460201	38.0	35.0	40.0	31.0	41.0
22	36.70247470185394	38.0	35.0	40.0	31.0	41.0
23	36.65942706613132	38.0	35.0	40.0	31.0	41.0
24	36.40593228095168	38.0	35.0	40.0	30.0	41.0
25	36.446035264202976	38.0	35.0	40.0	31.0	41.0
26	36.35475899611226	38.0	35.0	40.0	30.0	41.0
27	36.28317210697843	38.0	35.0	40.0	30.0	41.0
28	36.14952069812139	38.0	35.0	40.0	30.0	41.0
29	35.87449015730015	38.0	35.0	40.0	29.0	41.0
30	35.965826114292305	38.0	35.0	40.0	30.0	41.0
31	35.82748315778164	38.0	34.0	40.0	29.0	41.0
32	35.719748669932315	38.0	34.0	40.0	29.0	41.0
33	35.69140837952591	38.0	34.0	40.0	29.0	41.0
34	35.6808752780508	38.0	34.0	40.0	29.0	41.0
35	35.66088745458669	38.0	34.0	40.0	29.0	41.0
36	35.43593590207837	38.0	34.0	40.0	28.0	41.0
37	35.42077890037127	38.0	34.0	40.0	28.0	41.0
38	35.33754073767523	38.0	34.0	40.0	27.0	41.0
39	35.22600605643387	38.0	34.0	40.0	27.0	41.0
40	35.043560960275045	38.0	34.0	40.0	26.0	41.0
41	35.010982757866	38.0	34.0	40.0	26.0	41.0
42	34.90979017361512	37.0	34.0	40.0	26.0	41.0
43	34.74099393958687	37.0	33.0	40.0	25.0	41.0
44	34.66836846356789	37.0	33.0	40.0	25.0	41.0
45	34.457770102227194	37.0	33.0	40.0	24.0	41.0
46	34.33282133520093	37.0	33.0	40.0	24.0	41.0
47	34.171474276073106	37.0	33.0	40.0	23.0	41.0
48	34.04205282069852	37.0	33.0	40.0	23.0	41.0
49	33.874303132075624	36.0	33.0	40.0	23.0	41.0
50	33.69867848772199	36.0	33.0	40.0	22.0	41.0
51	31.688304556650735	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	23.0
9	28.0
10	27.0
11	13.0
12	13.0
13	17.0
14	19.0
15	35.0
16	48.0
17	110.0
18	189.0
19	365.0
20	637.0
21	934.0
22	1320.0
23	1755.0
24	2125.0
25	2398.0
26	2871.0
27	3453.0
28	4442.0
29	5539.0
30	6826.0
31	8884.0
32	11606.0
33	15592.0
34	22968.0
35	24770.0
36	26747.0
37	36308.0
38	44893.0
39	26345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.813098132533234	32.83406883324115	20.702896503424153	23.64993653080146
2	24.691706824033137	31.165963000839625	21.205477053596656	22.936853121530582
3	20.648778566113418	32.95145700608429	20.787654743477	25.612109684325297
4	21.214231425808684	32.480312610673174	19.5286168489831	26.776839114535044
5	17.568831251517093	35.81612634946658	19.342785402482264	27.272256996534068
6	20.58908966466775	36.625109927060166	22.013266853161323	20.772533555110762
7	79.46184486456588	9.998686844168194	5.836380783357143	4.703087507908779
8	83.36709072315094	6.104184987843361	4.914784145036072	5.61394014396963
9	76.29514967986853	10.38825640760357	8.708610720922552	4.607983191605353
10	35.64342646128379	33.34341412557749	13.785350751881195	17.227808661257527
11	28.747368714261267	30.156424714388606	24.414352395315614	16.68185417603451
12	28.94513794105124	24.482397742963673	29.325953132274584	17.2465111837105
13	25.278647688248846	30.47516344810846	24.967071622702473	19.27911724094022
14	18.150201151597873	33.894541648925795	28.523336370835207	19.431920828641122
15	16.402908043278433	30.47237796604099	32.90330795891812	20.221406031762452
16	19.004946220299797	28.79591568743708	30.777985141442798	21.421152950820325
17	19.053493193475603	27.121045112871712	27.452517478899974	26.372944214752707
18	22.80752716839831	26.56315284735956	27.548417647222674	23.080902337019456
19	23.60735844777022	30.473969670079548	25.575500491438625	20.34317139071161
20	25.066553125111916	28.910916304222393	25.270689168056094	20.7518414026096
21	21.624493141745223	30.86831434563057	26.671786647990675	20.83540586463353
22	20.99935138060429	27.896204979646082	26.385677847061118	24.718765792688508
23	20.087304966514527	31.350600669311547	24.35864275396633	24.203451610207598
24	17.687015276379512	30.17353553280303	28.213749935337024	23.92569925548044
25	19.227784785696947	29.456074937426134	27.589801952225002	23.726338324651913
26	21.19712060739426	32.629136938277696	23.7577744794133	22.415967974914743
27	17.99023489572349	30.668555488792414	26.296940346911896	25.0442692685722
28	19.588703676438403	29.145294723899042	30.496651452628896	20.76935014703366
29	18.454216622961127	33.041786210272065	27.57229320780094	20.93170395896587
30	22.01923574330589	27.971015069457984	27.218934911242602	22.79081427599352
31	24.872365232408686	30.629558739847912	24.211410130400353	20.28666589734305
32	25.53332033441702	29.327942762322774	23.66346601512915	21.475270888131064
33	23.920526217355146	27.917692984166525	26.244414113639706	21.917366684838623
34	21.784459397619607	27.882277569308762	26.581855369812533	23.751407663259094
35	23.24922503909623	27.55836579746362	26.75574903602424	22.43666012741591
36	24.396445724881914	28.145704587688964	27.219332837252242	20.238516850176875
37	21.932487873204856	31.01315941313872	24.839337373608753	22.21501534004767
38	23.265937931501014	29.84007353672658	25.707611926638364	21.18637660513404
39	23.55562806651731	27.687293824586256	25.922889897852393	22.834188211044037
40	26.99569841983582	26.349864506193722	25.07252201525648	21.58191505871398
41	23.391284624536915	24.149333672896862	28.652264397957843	23.80711730460838
42	26.62801478693052	25.718753854908215	24.736274537112568	22.916956821048696
43	23.404814108864596	25.72750822712025	27.22848513547391	23.63919252854124
44	22.945209567732977	25.842906769915203	26.530522914569264	24.68136074778256
45	23.568361698825722	27.282603072784646	27.010819608202052	22.13821562018758
46	21.459751773755187	27.870339789019628	28.493491920112373	22.176416517112806
47	22.439047683473735	27.69485441876937	26.318030425422695	23.548067472334193
48	22.88949992638369	24.43862588190352	30.814992260339114	21.85688193137368
49	23.38093854828633	23.924107551441885	29.25830571063616	23.43664818963562
50	21.47328125808287	26.375729696820173	26.698049764626763	25.45293928047019
51	21.440253399282938	25.19229774415745	28.536467929153254	24.830980927406358
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1750.0
1	1390.0
2	1030.0
3	714.0
4	398.0
5	341.5
6	285.0
7	305.5
8	326.0
9	372.5
10	419.0
11	425.5
12	432.0
13	553.0
14	674.0
15	627.5
16	581.0
17	562.5
18	544.0
19	538.0
20	532.0
21	625.5
22	719.0
23	821.0
24	923.0
25	1033.0
26	1525.0
27	1907.0
28	2401.5
29	2896.0
30	3504.0
31	4112.0
32	4683.5
33	5255.0
34	5905.0
35	6555.0
36	7204.0
37	7853.0
38	8704.5
39	9556.0
40	10717.5
41	11879.0
42	14519.0
43	17159.0
44	19896.0
45	22633.0
46	23924.5
47	25216.0
48	25615.5
49	26015.0
50	24543.5
51	23072.0
52	20768.5
53	18465.0
54	16069.5
55	13674.0
56	12382.0
57	11090.0
58	9840.5
59	8591.0
60	8105.5
61	7620.0
62	6670.5
63	5721.0
64	4865.5
65	4010.0
66	3414.0
67	2818.0
68	2259.5
69	1701.0
70	1470.5
71	1240.0
72	1057.5
73	875.0
74	748.5
75	533.5
76	445.0
77	344.5
78	244.0
79	182.0
80	120.0
81	119.5
82	119.0
83	80.5
84	42.0
85	29.5
86	17.0
87	16.0
88	15.0
89	11.0
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	251303.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.17702136464746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.20742130498229	30.620804367635884
2	7.873671044402751	6.011866153607398
3	2.5880758807588076	2.9641508457917336
4	1.2737127371273713	1.9450623351094098
5	0.8380237648530332	1.5996625587438271
6	0.5993329164060871	1.3728447332502995
7	0.48988951427975824	1.3091765717082566
8	0.4763393787784032	1.4548174912356797
9	0.3825307483844069	1.3143496098335476
>10	5.0093808630394	42.16583168525644
>50	0.225140712945591	5.188955165676494
>100	0.031269543464665414	1.9912217522273907
>500	0.0031269543464665416	0.8615098108657676
>1k	0.0020846362309776944	1.1997469190578705
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	1565	0.6227542050831069	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1450	0.5769927139747636	No Hit
AATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	775	0.3083926574692702	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCC	764	0.30401547136325474	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGT	626	0.24910168203324273	No Hit
AAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	345	0.13728447332502994	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	328	0.13051973116118787	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	316	0.1257446190455347	No Hit
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	266	0.10584831856364628	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	257	0.10226698447690637	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8443989924513436	0.0
2	0.0	0.0	0.0	1.1647294302097468	0.0
3	0.0	0.0	0.0	1.5654409219149792	0.0
4	0.0	0.0	0.0	3.2844812835501367	0.0
5	0.0	0.0	0.0	3.587302976884478	0.0
6	0.0	0.0	0.0	4.826444570896488	0.0
7	0.0	0.0	0.0	5.746847431188645	0.0
8	0.0	0.0	0.0	6.418944461466835	0.0
9	0.0	0.0	0.0	7.6751968738932685	0.0
10	0.0	0.0	0.0	9.195274230709542	0.0
11	0.0	0.0	0.0	11.01737742884088	0.0
12	0.0	0.0	0.0	12.046812015773787	0.0
13	0.0	0.0	0.0	12.79531083990243	0.0
14	0.0	0.0	0.0	13.232631524494336	0.0
15	0.0	0.0	0.0	13.535851143838315	0.0
16	0.0	0.0	0.0	14.091355853292638	0.0
17	0.0	0.0	0.0	14.69540753592277	0.0
18	0.0	0.0	0.0	15.669132481506388	0.0
19	0.0	0.0	0.0	16.004584107631025	0.0
20	0.0	0.0	0.0	16.450261238425327	0.0
21	0.0	0.0	0.0	16.88081718085339	0.0
22	0.0	0.0	0.0	17.322912977560954	0.0
23	0.0	0.0	0.0	17.76898803436489	0.0
24	0.0	0.0	0.0	18.066238763564304	0.0
25	0.0	0.0	0.0	18.36269364074444	0.0
26	0.0	0.0	0.0	18.60542850662348	0.0
27	0.0	0.0	0.0	18.88636426942774	0.0
28	0.0	0.0	0.0	19.161729068097078	0.0
29	0.0	0.0	0.0	19.43948142282424	0.0
30	0.0	0.0	0.0	19.859691289001724	0.0
31	0.0	0.0	0.0	20.128291345507215	0.0
32	0.0	0.0	0.0	20.406441626244018	0.0
33	0.0	0.0	0.0	20.716425987751837	0.0
34	0.0	0.0	0.0	21.009299530845233	0.0
35	0.0	0.0	0.0	21.36106612336502	0.0
36	0.0	0.0	0.0	21.607780249340436	0.0
37	0.0	0.0	0.0	21.893093198250718	0.0
38	0.0	0.0	0.0	22.198700373652525	0.0
39	0.0	0.0	0.0	22.52698933160368	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATGGG	90	0.0	45.000004	3
ACGTGGG	45	3.8198777E-10	45.000004	3
TTAACGG	65	0.0	45.000004	2
GTTTGGC	20	7.0227584E-4	45.0	26
ACCGGGT	20	7.0227584E-4	45.0	4
TAGACGG	40	6.7793735E-9	45.0	2
TAGCGTT	20	7.0227584E-4	45.0	15
CGTTGAA	20	7.0227584E-4	45.0	18
AGCCACG	25	3.8820803E-5	45.0	1
ATTAACG	25	3.8820803E-5	45.0	1
CGCTGCG	20	7.0227584E-4	45.0	1
GCGTTGA	20	7.0227584E-4	45.0	17
GCGCAAA	20	7.0227584E-4	45.0	39
AGAACGG	20	7.0227584E-4	45.0	2
ACGATGT	20	7.0227584E-4	45.0	27
TACGCAG	20	7.0227584E-4	45.0	1
GTTCGGC	25	3.8820803E-5	45.0	31
TCAGCCG	20	7.0227584E-4	45.0	29
ATCCACC	35	1.2068631E-7	45.0	25
CGCAGTT	20	7.0227584E-4	45.0	3
>>END_MODULE