SRR3548631_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548631_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	250036
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGT	5494	2.1972835911628725	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC	5320	2.1276936121198546	No Hit
AATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT	5304	2.121294533587163	Illumina PCR Primer Index 11 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT	3174	1.2694172039226352	Illumina PCR Primer Index 11 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2749	1.0994416803980227	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT	1226	0.4903293925674703	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT	1037	0.41474027740005437	No Hit
AACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG	873	0.34914972243996867	Illumina PCR Primer Index 11 (95% over 22bp)
AAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT	701	0.28035962821353727	Illumina PCR Primer Index 11 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	681	0.2723607800476731	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTAT	550	0.2199683245612632	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	504	0.2015709737797757	No Hit
AATGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC	500	0.19997120414660288	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT	496	0.19837143451343006	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	401	0.1603769057255755	No Hit
AATCTGACTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT	333	0.13318082196163752	Illumina PCR Primer Index 11 (95% over 21bp)
AATCTGTCACTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT	328	0.13118110992017148	Illumina PCR Primer Index 11 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT	306	0.12238237693772097	No Hit
AATGATACAGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC	296	0.1183829528547889	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	281	0.11238381673039081	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGGTTC	35	1.2068267E-7	45.000004	28
ACGGGCG	35	1.2068267E-7	45.000004	5
CCATGAG	35	1.2068267E-7	45.000004	1
GCGGCTA	70	0.0	45.000004	23
TTGATCG	20	7.0226885E-4	45.0	19
AGCAACG	20	7.0226885E-4	45.0	1
AATCCGG	25	3.8820228E-5	45.0	2
CAAACTA	25	3.8820228E-5	45.0	16
TTGGCAA	20	7.0226885E-4	45.0	17
CGAACCG	20	7.0226885E-4	45.0	24
TCCGCCG	20	7.0226885E-4	45.0	27
TTGTGTC	20	7.0226885E-4	45.0	13
TACCATA	25	3.8820228E-5	45.0	42
GGGCTAT	20	7.0226885E-4	45.0	15
TACCACG	20	7.0226885E-4	45.0	1
GCGAACC	20	7.0226885E-4	45.0	23
TAGAATG	40	6.7775545E-9	45.0	1
CGCGCCG	20	7.0226885E-4	45.0	14
ATAGACG	20	7.0226885E-4	45.0	1
TACGGAA	20	7.0226885E-4	45.0	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content