##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548631_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	250036
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.854328976627365	31.0	31.0	33.0	28.0	34.0
2	31.02103297125214	31.0	31.0	34.0	28.0	34.0
3	31.230758770737015	31.0	31.0	34.0	28.0	34.0
4	35.20289878257531	35.0	35.0	37.0	33.0	37.0
5	34.75983858324401	35.0	35.0	37.0	32.0	37.0
6	34.7746044569582	35.0	35.0	37.0	32.0	37.0
7	32.28885840438977	35.0	35.0	37.0	25.0	37.0
8	33.69286422755123	35.0	35.0	37.0	26.0	37.0
9	34.09459037898542	37.0	34.0	39.0	28.0	39.0
10	34.734214273144666	37.0	34.0	39.0	27.0	39.0
11	35.64977043305764	37.0	35.0	39.0	30.0	39.0
12	35.38842006751028	37.0	35.0	39.0	30.0	39.0
13	35.30939944647971	37.0	35.0	39.0	30.0	39.0
14	36.23838567246317	38.0	35.0	40.0	30.0	41.0
15	36.48761778303924	38.0	35.0	40.0	31.0	41.0
16	36.448919355612794	38.0	35.0	40.0	31.0	41.0
17	36.40966100881473	38.0	35.0	40.0	31.0	41.0
18	36.16132476923323	38.0	35.0	40.0	30.0	41.0
19	36.09174278903838	38.0	34.0	40.0	30.0	41.0
20	36.17448287446608	38.0	34.0	40.0	30.0	41.0
21	36.31311891087683	38.0	34.0	40.0	31.0	41.0
22	36.27530835559679	38.0	35.0	40.0	31.0	41.0
23	36.25779087811355	38.0	35.0	40.0	31.0	41.0
24	35.92190724535667	38.0	34.0	40.0	30.0	41.0
25	35.991021292933816	38.0	34.0	40.0	30.0	41.0
26	35.87324225311555	38.0	34.0	40.0	30.0	41.0
27	35.73449023340639	38.0	34.0	40.0	29.0	41.0
28	35.71585691660401	38.0	34.0	40.0	29.0	41.0
29	35.4679446159753	37.0	34.0	40.0	29.0	41.0
30	35.359892175526724	37.0	34.0	40.0	27.0	41.0
31	35.289310339311136	37.0	34.0	40.0	28.0	41.0
32	35.241129277384054	37.0	34.0	40.0	27.0	41.0
33	35.12691372442368	37.0	34.0	40.0	27.0	41.0
34	35.189104768913275	37.0	34.0	40.0	27.0	41.0
35	35.038654433761536	37.0	34.0	40.0	27.0	41.0
36	34.823185461293576	37.0	34.0	40.0	26.0	41.0
37	34.75795085507687	37.0	33.0	40.0	25.0	41.0
38	34.581452270872994	37.0	33.0	40.0	25.0	41.0
39	34.56211505543202	37.0	33.0	40.0	25.0	41.0
40	34.332684093490535	37.0	33.0	40.0	24.0	41.0
41	34.351793341758786	37.0	33.0	40.0	24.0	41.0
42	34.409868978867046	37.0	33.0	40.0	24.0	41.0
43	34.29399766433634	37.0	33.0	40.0	24.0	41.0
44	34.20619030859556	37.0	33.0	40.0	23.0	41.0
45	33.904889695883796	36.0	33.0	40.0	23.0	40.0
46	33.76969316418436	36.0	33.0	39.0	23.0	40.0
47	33.72463965189013	36.0	33.0	39.0	23.0	40.0
48	33.694627973571805	36.0	33.0	39.0	23.0	40.0
49	33.594962325425136	36.0	33.0	39.0	22.0	40.0
50	33.3566406437473	36.0	32.0	39.0	21.0	40.0
51	31.09754195395863	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	6.0
11	5.0
12	12.0
13	3.0
14	10.0
15	19.0
16	48.0
17	114.0
18	186.0
19	388.0
20	673.0
21	1057.0
22	1480.0
23	1954.0
24	2424.0
25	2801.0
26	3281.0
27	3878.0
28	4962.0
29	6330.0
30	8022.0
31	10580.0
32	13696.0
33	18445.0
34	27115.0
35	28342.0
36	28257.0
37	35113.0
38	34101.0
39	16729.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.26177030507607	40.38898398630597	18.54053016365643	22.808715544961526
2	19.467596666080084	40.67774240509367	19.496392519477197	20.358268409349055
3	19.740757330944344	30.411620726615368	27.271272936697116	22.576349005743175
4	24.54926490585356	30.325233166424038	17.82263354077013	27.302868386952277
5	15.93890479770913	40.82132172967093	18.782495320673824	24.45727815194612
6	20.47505159257067	35.6132716888768	25.052392455486412	18.85928426306612
7	62.94973523812571	20.6850213569246	9.45783807131773	6.9074053336319565
8	69.40560559279464	8.433985506087122	7.550912668575725	14.609496232542513
9	63.86880289238349	14.989041578012765	13.195299876817737	7.946855652785999
10	38.905597593946474	25.887872146410917	16.325649106528658	18.88088115311395
11	32.19416404037819	23.00268761298373	26.346206146314934	18.456942200323155
12	29.162600585515687	22.479163000527926	32.09417843830488	16.264057975651504
13	23.522212801356606	32.77847989889456	22.742725047593147	20.95658225215569
14	14.129565342590666	33.64235550080789	34.86497944296021	17.363099713641237
15	13.485658065238606	30.598793773696585	32.06138316082484	23.854165000239966
16	14.727879185397303	26.75094786350766	35.94362411812699	22.577548832968052
17	14.297541154073814	27.877185685261324	29.225791486026015	28.599481674638856
18	17.219520389063973	28.23793373754179	27.152889983842325	27.389655889551907
19	19.59557823673391	32.42933017645459	28.834647810715257	19.14044377609624
20	22.60194531987394	32.5229166999952	24.962005471212144	19.913132508918714
21	17.881025132380937	30.54200195171895	25.727895183093636	25.849077732806474
22	17.06194308019645	33.207618102993166	25.95146298932954	23.778975827480842
23	21.571293733702348	28.925834679806105	21.382920859396247	28.1199507270953
24	15.201011054408164	36.10400102385257	25.53152346062167	23.1634644611176
25	16.692396294933527	26.37980130861156	27.11609528227935	29.811707114175555
26	20.325073189460717	33.592362699771236	22.783519173239053	23.299044937528997
27	14.618694907933257	31.461469548385036	27.572029627733606	26.347805915948104
28	18.016205666384042	28.14514709881777	27.228479099009746	26.61016813578845
29	16.171671279335776	29.87049864819466	31.68303764257947	22.274792429890095
30	19.29762114255547	33.78473499816027	21.828456702234877	25.089187157049388
31	22.909501031851416	33.187221040170215	19.964325137180246	23.938952790798126
32	21.297733126429794	27.637220240285398	19.308019645171097	31.75702698811371
33	21.682477723207857	32.53571485706058	21.06416675998656	24.717640659744998
34	15.245804604137003	27.11849493672911	23.686989073573407	33.94871138556048
35	16.880369226831338	29.388967988609636	23.092674654849702	30.63798812970932
36	18.91407637300229	29.49255307235758	29.650530323633394	21.942840231006734
37	16.817578268729303	32.13977187285031	20.639027979970884	30.403621878449506
38	19.979123006287093	33.86712313426867	22.49356092722648	23.66019293221776
39	16.915164216352846	27.487241837175446	25.40994096850054	30.187652977971176
40	20.007918859684207	30.91994752755603	20.165096226143437	28.90703738661633
41	16.544417603865043	21.905645587035465	30.737573789374327	30.812363019725158
42	23.252651618166983	23.469820345870197	21.7976611367963	31.47986689916652
43	20.392263514053976	24.076532979250988	26.964517109536228	28.566686397158808
44	19.08565166616007	24.73683789534307	25.49312898942552	30.684381449071335
45	24.326896926842533	29.79930889951847	23.054280183653553	22.819513989985442
46	17.855828760658465	24.939208753939432	33.69514789870259	23.509814586699516
47	20.909389047977093	26.892127533635158	23.04388168103793	29.15460173734982
48	19.198435425298758	21.181749828024763	35.49808827528836	24.12172647138812
49	21.587291430034075	20.55384024700443	29.126205826361	28.73266249660049
50	18.964469116447233	22.753923435025357	26.085843638516053	32.19576381001136
51	17.290310195331873	23.20945783807132	33.246412516597616	26.2538194499992
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3148.0
1	2501.0
2	1854.0
3	1225.0
4	596.0
5	555.0
6	514.0
7	535.5
8	557.0
9	606.0
10	655.0
11	699.5
12	744.0
13	783.5
14	823.0
15	828.0
16	833.0
17	832.0
18	831.0
19	830.0
20	829.0
21	838.0
22	847.0
23	725.5
24	604.0
25	619.0
26	901.0
27	1168.0
28	1385.0
29	1602.0
30	2087.5
31	2573.0
32	3136.0
33	3699.0
34	4457.5
35	5216.0
36	5521.5
37	5827.0
38	6130.0
39	6433.0
40	7941.5
41	9450.0
42	12911.0
43	16372.0
44	26298.5
45	36225.0
46	34125.0
47	32025.0
48	29836.5
49	27648.0
50	24296.0
51	20944.0
52	18316.0
53	15688.0
54	13771.5
55	11855.0
56	10356.0
57	8857.0
58	8217.0
59	7577.0
60	6892.0
61	6207.0
62	5674.5
63	5142.0
64	4472.0
65	3802.0
66	3345.0
67	2888.0
68	2328.5
69	1769.0
70	1460.0
71	1151.0
72	1001.5
73	852.0
74	749.5
75	526.5
76	406.0
77	336.0
78	266.0
79	184.0
80	102.0
81	104.0
82	106.0
83	72.5
84	39.0
85	29.0
86	19.0
87	12.5
88	6.0
89	4.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	250036.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.93882480922747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.25439572668596	28.483098433825525
2	8.968395281549077	6.446271736869891
3	3.359670598709103	3.6222783919115646
4	1.6258624527042065	2.3372634340654943
5	1.0071221900734477	1.8097393975267562
6	0.6632539505898064	1.4301940520565037
7	0.4707322501669263	1.1842294709561823
8	0.3594480302693078	1.0334511830296438
9	0.29601602492766527	0.9574621254539346
>10	3.572223458713555	30.302436449151323
>50	0.36167371466726017	8.087635380505208
>100	0.047852214555975964	3.2623302244476795
>500	0.005564210994880926	1.323409429042218
>1k	0.00445136879590474	3.273928554288182
>5k	0.0033385265969285557	6.446271736869891
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGT	5494	2.1972835911628725	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC	5320	2.1276936121198546	No Hit
AATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT	5304	2.121294533587163	Illumina PCR Primer Index 11 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT	3174	1.2694172039226352	Illumina PCR Primer Index 11 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2749	1.0994416803980227	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT	1226	0.4903293925674703	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT	1037	0.41474027740005437	No Hit
AACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG	873	0.34914972243996867	Illumina PCR Primer Index 11 (95% over 22bp)
AAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT	701	0.28035962821353727	Illumina PCR Primer Index 11 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	681	0.2723607800476731	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTAT	550	0.2199683245612632	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	504	0.2015709737797757	No Hit
AATGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC	500	0.19997120414660288	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT	496	0.19837143451343006	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	401	0.1603769057255755	No Hit
AATCTGACTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT	333	0.13318082196163752	Illumina PCR Primer Index 11 (95% over 21bp)
AATCTGTCACTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT	328	0.13118110992017148	Illumina PCR Primer Index 11 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT	306	0.12238237693772097	No Hit
AATGATACAGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC	296	0.1183829528547889	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	281	0.11238381673039081	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.622966292853829	0.0
2	0.0	0.0	0.0	1.9277224079732518	0.0
3	0.0	0.0	0.0	2.6988113711625528	0.0
4	0.0	0.0	0.0	6.9134044697563555	0.0
5	0.0	0.0	0.0	7.415332192164328	0.0
6	0.0	0.0	0.0	9.51143035402902	0.0
7	0.0	0.0	0.0	11.250379945287879	0.0
8	0.0	0.0	0.0	12.317826233022444	0.0
9	0.0	0.0	0.0	16.155273640595755	0.0
10	0.0	0.0	0.0	18.2069781951399	0.0
11	0.0	0.0	0.0	22.56355084867779	0.0
12	0.0	0.0	0.0	23.72098417827833	0.0
13	0.0	0.0	0.0	24.33129629333376	0.0
14	0.0	0.0	0.0	25.051192628261532	0.0
15	0.0	0.0	0.0	25.473531811419157	0.0
16	0.0	0.0	0.0	26.097441968356556	0.0
17	0.0	0.0	0.0	26.784942968212576	0.0
18	0.0	0.0	0.0	27.98197059623414	0.0
19	0.0	0.0	0.0	28.37991329248588	0.0
20	3.999424082932058E-4	0.0	0.0	28.811851093442545	0.0
21	3.999424082932058E-4	0.0	0.0	29.234990161416757	0.0
22	3.999424082932058E-4	0.0	0.0	29.634132684893377	0.0
23	3.999424082932058E-4	0.0	0.0	30.068470140299798	0.0
24	3.999424082932058E-4	0.0	0.0	30.36442752243677	0.0
25	3.999424082932058E-4	0.0	0.0	30.653185941224464	0.0
26	3.999424082932058E-4	0.0	0.0	30.888752019709163	0.0
27	3.999424082932058E-4	0.0	0.0	31.162312626981716	0.0
28	3.999424082932058E-4	0.0	0.0	31.427074501271818	0.0
29	3.999424082932058E-4	0.0	0.0	31.70703418707706	0.0
30	3.999424082932058E-4	0.0	0.0	32.14097170007519	0.0
31	3.999424082932058E-4	0.0	0.0	32.40293397750724	0.0
32	3.999424082932058E-4	0.0	0.0	32.65089827064903	0.0
33	3.999424082932058E-4	0.0	0.0	32.90966100881473	0.0
34	3.999424082932058E-4	0.0	0.0	33.14002783599162	0.0
35	3.999424082932058E-4	0.0	0.0	33.43918475739493	0.0
36	3.999424082932058E-4	0.0	0.0	33.680350029595736	0.0
37	3.999424082932058E-4	0.0	0.0	33.90791725991457	0.0
38	3.999424082932058E-4	0.0	0.0	34.14988241693196	0.0
39	3.999424082932058E-4	0.0	0.0	34.39784671007375	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGGTTC	35	1.2068267E-7	45.000004	28
ACGGGCG	35	1.2068267E-7	45.000004	5
CCATGAG	35	1.2068267E-7	45.000004	1
GCGGCTA	70	0.0	45.000004	23
TTGATCG	20	7.0226885E-4	45.0	19
AGCAACG	20	7.0226885E-4	45.0	1
AATCCGG	25	3.8820228E-5	45.0	2
CAAACTA	25	3.8820228E-5	45.0	16
TTGGCAA	20	7.0226885E-4	45.0	17
CGAACCG	20	7.0226885E-4	45.0	24
TCCGCCG	20	7.0226885E-4	45.0	27
TTGTGTC	20	7.0226885E-4	45.0	13
TACCATA	25	3.8820228E-5	45.0	42
GGGCTAT	20	7.0226885E-4	45.0	15
TACCACG	20	7.0226885E-4	45.0	1
GCGAACC	20	7.0226885E-4	45.0	23
TAGAATG	40	6.7775545E-9	45.0	1
CGCGCCG	20	7.0226885E-4	45.0	14
ATAGACG	20	7.0226885E-4	45.0	1
TACGGAA	20	7.0226885E-4	45.0	4
>>END_MODULE