SRR3548630_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548630_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	263964
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	16718	6.3334394084041765	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCC	14159	5.363989028806959	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGT	13582	5.145398614962646	No Hit
CTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGCT	3712	1.406252367747117	No Hit
AATGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTT	3509	1.3293479413859466	No Hit
AACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTG	3320	1.2577472685669258	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCT	3095	1.172508372353806	No Hit
AATGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTC	1796	0.6803958115500598	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTAT	1626	0.6159930899668137	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1527	0.5784879756330409	No Hit
AATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCC	559	0.2117713021472625	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGG	511	0.1935870042884636	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATG	486	0.18411601582033915	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGC	468	0.17729690412328955	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGAGAACTTC	433	0.16403752026791532	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTA	418	0.15835492718704064	No Hit
AATCTGACTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	403	0.15267233410616599	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGCACTTCGTATGCC	394	0.1492627782576412	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCCGAGAACTTCGTATGCCGT	376	0.1424436665605916	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	360	0.13638223394099194	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	311	0.11781909654346805	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGC	300	0.11365186161749329	No Hit
AATCTGTCTCTTATACCCATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	298	0.11289418254004334	No Hit
AAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	297	0.11251534300131835	No Hit
AACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTT	292	0.11062114530769346	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	292	0.11062114530769346	No Hit
AATCTGTATCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	272	0.10304435453319391	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGA	90	0.0	45.000004	4
TTAGGGA	90	0.0	45.000004	4
AATCCGG	30	2.1591277E-6	45.000004	2
TCCTATA	60	0.0	45.000004	16
CCTATAC	60	0.0	45.000004	17
GTTTGTC	25	3.8826154E-5	45.0	36
TTCTACG	35	1.2071723E-7	45.0	1
CTAGACG	20	7.0234045E-4	45.0	1
CGGGTCA	20	7.0234045E-4	45.0	6
TAATAGC	20	7.0234045E-4	45.0	17
TAGACGG	20	7.0234045E-4	45.0	2
CGGCCGT	20	7.0234045E-4	45.0	34
CGGCCAG	20	7.0234045E-4	45.0	42
GCGCGAC	25	3.8826154E-5	45.0	9
GGCCGTG	20	7.0234045E-4	45.0	35
GACCGAT	20	7.0234045E-4	45.0	9
CACGCCG	35	1.2071723E-7	45.0	26
CGACCCG	25	3.8826154E-5	45.0	32
TATGCCA	20	7.0234045E-4	45.0	10
AAACGCG	20	7.0234045E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content