##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548630_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	263964
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.860962100892547	31.0	30.0	33.0	28.0	34.0
2	31.020665696837447	31.0	31.0	34.0	28.0	34.0
3	31.09227773484263	31.0	31.0	34.0	28.0	34.0
4	34.95265263445015	35.0	35.0	37.0	32.0	37.0
5	34.40470670242912	35.0	35.0	37.0	30.0	37.0
6	34.434945674410145	35.0	35.0	37.0	30.0	37.0
7	33.27926914276189	35.0	33.0	37.0	28.0	37.0
8	33.98229303995999	35.0	35.0	37.0	29.0	37.0
9	35.55607961691746	37.0	35.0	39.0	31.0	39.0
10	35.32255156006122	37.0	34.0	39.0	30.0	39.0
11	35.75886484520616	37.0	35.0	39.0	30.0	39.0
12	34.943090724492734	37.0	33.0	39.0	29.0	39.0
13	35.33189374308618	37.0	34.0	39.0	30.0	39.0
14	36.14485687442227	38.0	34.0	40.0	30.0	41.0
15	36.41290100165174	38.0	35.0	40.0	31.0	41.0
16	36.115436195844886	38.0	34.0	40.0	30.0	41.0
17	35.967389492506555	38.0	34.0	40.0	30.0	41.0
18	35.5049021836311	37.0	34.0	40.0	27.0	41.0
19	35.32680213968572	37.0	33.0	40.0	27.0	41.0
20	35.81293661256838	37.0	34.0	40.0	30.0	41.0
21	36.25945583488657	38.0	34.0	40.0	31.0	41.0
22	36.22167795608492	38.0	34.0	40.0	30.0	41.0
23	36.262475186010214	38.0	35.0	40.0	31.0	41.0
24	35.91328741798124	38.0	34.0	40.0	30.0	41.0
25	35.77628009880135	37.0	34.0	40.0	29.0	41.0
26	35.81993756724402	38.0	34.0	40.0	30.0	41.0
27	35.5542270915731	38.0	34.0	40.0	29.0	41.0
28	35.58720886181449	38.0	34.0	40.0	29.0	41.0
29	35.348990771468834	37.0	34.0	40.0	27.0	41.0
30	35.35154793835523	37.0	34.0	40.0	27.0	41.0
31	35.31980876180085	37.0	34.0	40.0	28.0	41.0
32	34.4205194647755	37.0	33.0	40.0	25.0	41.0
33	34.87325923231956	37.0	33.0	40.0	26.0	41.0
34	34.863564728523585	37.0	33.0	40.0	26.0	41.0
35	34.790823748693	37.0	33.0	40.0	25.0	41.0
36	35.06786531496719	37.0	34.0	40.0	27.0	41.0
37	34.4240843448349	37.0	33.0	40.0	24.0	41.0
38	34.67474352563228	37.0	33.0	40.0	25.0	41.0
39	33.955111303056476	37.0	33.0	40.0	23.0	40.0
40	33.74596157051719	36.0	32.0	39.0	23.0	40.0
41	34.324752617781215	37.0	33.0	40.0	24.0	40.0
42	34.38407888954554	37.0	33.0	40.0	24.0	40.0
43	34.58311360640087	37.0	33.0	40.0	25.0	40.0
44	34.48474413177554	37.0	33.0	40.0	24.0	40.0
45	34.0800450061372	37.0	33.0	40.0	23.0	40.0
46	33.655415132366535	36.0	32.0	39.0	23.0	40.0
47	33.674398781652044	36.0	32.0	39.0	23.0	40.0
48	33.565327090057735	36.0	32.0	39.0	22.0	40.0
49	33.48189147004894	36.0	32.0	39.0	21.0	40.0
50	33.38137018684366	36.0	32.0	39.0	21.0	40.0
51	30.54406661514449	34.0	26.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	8.0
11	1.0
12	5.0
13	3.0
14	9.0
15	30.0
16	51.0
17	100.0
18	241.0
19	421.0
20	672.0
21	1098.0
22	1499.0
23	1982.0
24	2346.0
25	2858.0
26	3410.0
27	4150.0
28	5446.0
29	7236.0
30	9218.0
31	12219.0
32	16208.0
33	20573.0
34	28158.0
35	29447.0
36	30294.0
37	34214.0
38	34781.0
39	17283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.647073085723811	52.34804746101741	14.026533921292298	18.978345531966482
2	17.74598051249413	52.02868572987226	14.52584443333182	15.6994893243018
3	16.074919307178252	23.04177842433059	41.146898819536	19.736403448955162
4	33.21248352048006	24.38665878680426	16.40640390356261	25.994453789153066
5	14.00607658620115	43.922277280235186	21.17561485657135	20.896031276992318
6	21.217666045369825	27.502992832355925	34.939991817065966	16.339349305208287
7	54.696852601112276	23.88204452122259	13.61511418223697	7.805988695428165
8	58.0567804700641	6.055371186980043	8.893637011107575	26.994211331848284
9	62.3641860253671	8.851585822309103	16.758724674576836	12.025503477746966
10	35.80753436074616	25.120092133775817	18.96546498764983	20.106908517828188
11	28.795972178024275	19.680714036762588	29.09866496946553	22.424648815747602
12	23.625191313967058	21.648785440438846	40.64834598657392	14.077677259020168
13	23.13383643224076	33.84438787107333	20.591444287857435	22.430331408828476
14	13.303708081405041	28.53570941492022	43.07784394841721	15.082738555257535
15	11.480353381521722	32.16991710990892	24.84164507281296	31.508084435756388
16	14.145489536451942	27.390098649815886	35.59500537952145	22.869406434210727
17	13.493127850767529	30.30981497476929	28.28491764028428	27.912139534178905
18	16.168871512782047	29.96090375960358	25.09357336606507	28.776651361549305
19	16.989437953660346	32.55557576033095	33.42804321801458	17.02694306799412
20	19.607976845327393	37.273643375611826	25.22995560000606	17.888424179054716
21	15.10736312527466	26.647573153914927	27.877286296616205	30.36777742419421
22	17.770226242972527	34.41075298146717	27.74734433483354	20.071676440726765
23	24.33627312815384	25.855040838902276	18.80635238138534	31.002333651558544
24	13.835977633313634	42.98502826142958	22.57201739631162	20.60697670894516
25	16.854571077874255	23.13383643224076	26.656665302844328	33.35492718704066
26	27.30751163037384	31.60658271582489	16.024912488066555	25.060993165734725
27	13.959858162476701	27.475337546029007	29.99272628085648	28.572078010637814
28	24.695034171326395	22.332969647376157	19.8993044506069	33.07269173069055
29	24.562819172311375	33.01927535573033	24.276795320574017	18.14111015138428
30	24.390826021730234	32.000196996560135	18.429785879892712	25.179191101816915
31	36.4405752299556	29.557060811322756	14.937264172387144	19.0650997863345
32	20.786925489839522	33.04958251882832	18.355912169841343	27.80757982149081
33	25.98460396114622	31.96079768453274	17.21636283735661	24.838235516964435
34	23.31567941082875	23.785440438847722	18.737403585337393	34.16147656498613
35	16.1029534330439	30.566289342486098	28.67739540240336	24.653361822066646
36	30.693579427497692	23.073979785122216	27.30448091406404	18.92795987331606
37	17.693700656150078	35.89731933142398	19.34278916821991	27.066190844206027
38	30.976193723386526	29.68738161264415	22.298874088890912	17.03755057507842
39	17.942219393553664	29.2740676758952	26.545286478459186	26.23842645209195
40	19.652301071358217	35.115015683956905	25.255337849100634	19.977345395584244
41	18.36500431877074	19.187843796881392	35.03470170174721	27.412450182600658
42	26.55096907154006	20.874437423284995	25.993317270536892	26.581276234638057
43	23.521389280356413	20.687290691154857	28.167856222818262	27.62346380567047
44	19.538270370201996	19.818232789319758	25.986876998378566	34.65661984209968
45	32.46503311057569	26.892682335469985	21.379809367944112	19.262475186010214
46	16.12606264490612	20.14820202754921	42.11975875498174	21.605976572562927
47	23.178160658271583	26.54793835523026	20.616447697413285	29.657453289084874
48	17.62134230425361	17.92782349108212	40.36307981391402	24.087754390750256
49	23.739222015123275	17.94562894940219	32.31955872770529	25.99559030776924
50	21.285478322801595	20.134184964616388	23.342577018078224	35.237759694503794
51	16.26964283008289	19.235956418299466	38.30976951402464	26.184631237593003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1785.0
1	1431.0
2	1077.0
3	767.5
4	458.0
5	440.0
6	422.0
7	474.0
8	526.0
9	570.5
10	615.0
11	644.5
12	674.0
13	729.0
14	784.0
15	752.5
16	721.0
17	705.5
18	690.0
19	628.5
20	567.0
21	578.0
22	589.0
23	611.0
24	633.0
25	815.0
26	1259.0
27	1521.0
28	1866.5
29	2212.0
30	2521.0
31	2830.0
32	3034.0
33	3238.0
34	3622.5
35	4007.0
36	4642.5
37	5278.0
38	5817.5
39	6357.0
40	6967.0
41	7577.0
42	10525.0
43	13473.0
44	35095.0
45	56717.0
46	50583.5
47	44450.0
48	34784.0
49	25118.0
50	21162.0
51	17206.0
52	15350.0
53	13494.0
54	12403.5
55	11313.0
56	10032.5
57	8752.0
58	7910.5
59	7069.0
60	6484.0
61	5899.0
62	5321.5
63	4744.0
64	4089.5
65	3435.0
66	2996.0
67	2557.0
68	2126.0
69	1695.0
70	1656.0
71	1617.0
72	1335.0
73	1053.0
74	935.0
75	597.5
76	378.0
77	299.0
78	220.0
79	180.0
80	140.0
81	130.5
82	121.0
83	79.0
84	37.0
85	38.0
86	39.0
87	30.0
88	21.0
89	26.0
90	31.0
91	18.5
92	6.0
93	5.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	263964.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.386583018896516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.82983112027846	23.459259596005516
2	7.719479180095398	4.536982315770333
3	2.551244037643419	2.2491703414101925
4	1.3059172360448628	1.5350578109136095
5	0.910145674874307	1.337303571699171
6	0.7296635297150961	1.286539073510024
7	0.5775428645094752	1.18804079344153
8	0.5582054918138455	1.3123001621433226
9	0.4860126337501611	1.2854025548938492
>10	5.123114606162176	30.69888318103984
>50	0.12633750161144774	2.388204452122259
>100	0.06703622534484982	4.433937961237138
>500	0.0025783163594173004	0.4053583064357261
>1k	0.00902410725796055	7.040732827203709
>5k	0.0	0.0
>10k+	0.003867474539125951	16.84282705217378
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	16718	6.3334394084041765	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCC	14159	5.363989028806959	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGT	13582	5.145398614962646	No Hit
CTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGCT	3712	1.406252367747117	No Hit
AATGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTT	3509	1.3293479413859466	No Hit
AACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTG	3320	1.2577472685669258	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCT	3095	1.172508372353806	No Hit
AATGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTC	1796	0.6803958115500598	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTAT	1626	0.6159930899668137	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1527	0.5784879756330409	No Hit
AATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCC	559	0.2117713021472625	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGG	511	0.1935870042884636	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATG	486	0.18411601582033915	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGC	468	0.17729690412328955	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGAGAACTTC	433	0.16403752026791532	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTA	418	0.15835492718704064	No Hit
AATCTGACTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	403	0.15267233410616599	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGCACTTCGTATGCC	394	0.1492627782576412	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCCGAGAACTTCGTATGCCGT	376	0.1424436665605916	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	360	0.13638223394099194	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	311	0.11781909654346805	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGC	300	0.11365186161749329	No Hit
AATCTGTCTCTTATACCCATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	298	0.11289418254004334	No Hit
AAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	297	0.11251534300131835	No Hit
AACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTT	292	0.11062114530769346	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	292	0.11062114530769346	No Hit
AATCTGTATCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	272	0.10304435453319391	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7883953872497767E-4	0.0	0.0	1.763498052764771	0.0
2	3.7883953872497767E-4	0.0	0.0	1.9665560455213589	0.0
3	3.7883953872497767E-4	0.0	0.0	3.8285523783546243	0.0
4	3.7883953872497767E-4	0.0	0.0	12.507766210543862	0.0
5	3.7883953872497767E-4	0.0	0.0	13.550332621115	0.0
6	3.7883953872497767E-4	0.0	0.0	15.977178706187207	0.0
7	3.7883953872497767E-4	0.0	0.0	18.031246685154038	0.0
8	3.7883953872497767E-4	0.0	0.0	18.536618629813155	0.0
9	3.7883953872497767E-4	0.0	0.0	25.341334424391206	0.0
10	3.7883953872497767E-4	0.0	0.0	26.19031383067388	0.0
11	3.7883953872497767E-4	0.0	0.0	33.4844903092846	0.0
12	3.7883953872497767E-4	0.0	0.0	34.21375642133018	0.0
13	3.7883953872497767E-4	0.0	0.0	34.74072221969663	0.0
14	3.7883953872497767E-4	0.0	0.0	35.76283129517662	0.0
15	3.7883953872497767E-4	0.0	0.0	36.15720325498932	0.0
16	3.7883953872497767E-4	0.0	0.0	36.576578624357865	0.0
17	3.7883953872497767E-4	0.0	0.0	36.967541028322046	0.0
18	3.7883953872497767E-4	0.0	0.0	37.73999484778227	0.0
19	3.7883953872497767E-4	0.0	0.0	37.999499931808884	0.0
20	3.7883953872497767E-4	0.0	0.0	38.23475928535709	0.0
21	3.7883953872497767E-4	0.0	0.0	38.53896743495325	0.0
22	3.7883953872497767E-4	0.0	0.0	38.79013804912791	0.0
23	3.7883953872497767E-4	0.0	0.0	39.06441787516479	0.0
24	3.7883953872497767E-4	0.0	0.0	39.26141443530178	0.0
25	3.7883953872497767E-4	0.0	0.0	39.436059462654	0.0
26	3.7883953872497767E-4	0.0	0.0	39.62244851570669	0.0
27	3.7883953872497767E-4	0.0	0.0	39.78610719643588	0.0
28	3.7883953872497767E-4	0.0	0.0	39.95772150747829	0.0
29	3.7883953872497767E-4	0.0	0.0	40.15396038853783	0.0
30	3.7883953872497767E-4	0.0	0.0	40.44301495658499	0.0
31	3.7883953872497767E-4	0.0	0.0	40.624857935172976	0.0
32	3.7883953872497767E-4	0.0	0.0	40.83284084193299	0.0
33	3.7883953872497767E-4	0.0	0.0	41.020366413601856	0.0
34	3.7883953872497767E-4	0.0	0.0	41.18970768741192	0.0
35	3.7883953872497767E-4	0.0	0.0	41.413980694337106	0.0
36	3.7883953872497767E-4	0.0	0.0	41.57422981921777	0.0
37	3.7883953872497767E-4	0.0	0.0	41.748496007031264	0.0
38	3.7883953872497767E-4	0.0	0.0	41.9250352320771	0.0
39	3.7883953872497767E-4	0.0	0.0	42.13263929929839	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGA	90	0.0	45.000004	4
TTAGGGA	90	0.0	45.000004	4
AATCCGG	30	2.1591277E-6	45.000004	2
TCCTATA	60	0.0	45.000004	16
CCTATAC	60	0.0	45.000004	17
GTTTGTC	25	3.8826154E-5	45.0	36
TTCTACG	35	1.2071723E-7	45.0	1
CTAGACG	20	7.0234045E-4	45.0	1
CGGGTCA	20	7.0234045E-4	45.0	6
TAATAGC	20	7.0234045E-4	45.0	17
TAGACGG	20	7.0234045E-4	45.0	2
CGGCCGT	20	7.0234045E-4	45.0	34
CGGCCAG	20	7.0234045E-4	45.0	42
GCGCGAC	25	3.8826154E-5	45.0	9
GGCCGTG	20	7.0234045E-4	45.0	35
GACCGAT	20	7.0234045E-4	45.0	9
CACGCCG	35	1.2071723E-7	45.0	26
CGACCCG	25	3.8826154E-5	45.0	32
TATGCCA	20	7.0234045E-4	45.0	10
AAACGCG	20	7.0234045E-4	45.0	1
>>END_MODULE