FastQCFastQC Report
Sat 18 Jun 2016
SRR3548628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227718
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGT48822.1438797108704626No Hit
AATCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCT47622.0911829543558262No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCC46222.02970340508875No Hit
CTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGCT23321.0240736349344364No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22500.9880641846494348No Hit
AATGACTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTT12450.5467288488393539No Hit
AACTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTG9110.4000562098736156No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCT8930.39215169639642017No Hit
AATGCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTC6060.26611862039891443No Hit
AAACTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCT5780.25382271054549926No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTGCCATTCGTAT5690.2498704538069015No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4710.20683476931994835No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4550.19980853511799682No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTT3920.17214273794781265No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2580.11329802650646853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGG302.1578107E-645.0000042
GACACTA302.1578107E-645.00000425
GGGACGA302.1578107E-645.0000047
AATCTAT302.1578107E-645.00000436
ACGTAAG302.1578107E-645.0000041
TGGGCGA302.1578107E-645.0000046
GGATTCC302.1578107E-645.0000048
GGTTATT302.1578107E-645.0000049
ATACCGG302.1578107E-645.0000042
CTTGTAC207.021362E-445.034
GTTTGCG207.021362E-445.010
TTAGGTC207.021362E-445.032
GATACGA207.021362E-445.015
CGAAACG253.8809252E-545.01
ACGTTGT207.021362E-445.042
TTAGCGG207.021362E-445.02
CGGCCTA207.021362E-445.015
GACGTTG253.8809252E-545.041
CGCATGG207.021362E-445.02
ATCAGCG253.8809252E-545.01