##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548626_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	276903
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.912308642376573	31.0	31.0	33.0	28.0	34.0
2	31.082913511229563	31.0	31.0	34.0	28.0	34.0
3	31.248000202236884	31.0	31.0	34.0	28.0	34.0
4	35.17454126535285	35.0	35.0	37.0	32.0	37.0
5	34.688735766676416	35.0	35.0	37.0	32.0	37.0
6	34.71287779475123	35.0	35.0	37.0	32.0	37.0
7	33.53001953752758	35.0	35.0	37.0	29.0	37.0
8	34.29283539723296	35.0	35.0	37.0	30.0	37.0
9	35.71816845610196	38.0	35.0	39.0	31.0	39.0
10	35.47991534941839	37.0	35.0	39.0	30.0	39.0
11	35.91276367536646	37.0	35.0	39.0	31.0	39.0
12	35.49195927815878	37.0	35.0	39.0	30.0	39.0
13	35.54861810814617	37.0	35.0	39.0	30.0	39.0
14	36.496018461338444	38.0	35.0	40.0	31.0	41.0
15	36.67422527022098	38.0	35.0	40.0	31.0	41.0
16	36.5027608946093	38.0	35.0	40.0	31.0	41.0
17	36.36607765174086	38.0	35.0	40.0	30.0	41.0
18	36.05401891637144	38.0	35.0	40.0	30.0	41.0
19	35.95348551658884	38.0	34.0	40.0	29.0	41.0
20	36.16551644438666	38.0	34.0	40.0	30.0	41.0
21	36.42002434065358	38.0	35.0	40.0	31.0	41.0
22	36.391898968230755	38.0	35.0	40.0	31.0	41.0
23	36.369959877646686	38.0	35.0	40.0	31.0	41.0
24	36.012686753122935	38.0	34.0	40.0	30.0	41.0
25	36.03113364607823	38.0	34.0	40.0	30.0	41.0
26	35.99805708136062	38.0	34.0	40.0	30.0	41.0
27	35.88155419045658	38.0	34.0	40.0	30.0	41.0
28	35.6854313604403	38.0	34.0	40.0	29.0	41.0
29	35.583312567938954	38.0	34.0	40.0	29.0	41.0
30	35.554071281279	38.0	34.0	40.0	29.0	41.0
31	35.33181294532743	38.0	34.0	40.0	27.0	41.0
32	35.26531673546332	38.0	34.0	40.0	27.0	41.0
33	35.212879600437695	38.0	34.0	40.0	27.0	41.0
34	35.156466343809925	38.0	34.0	40.0	27.0	41.0
35	35.18347580199565	38.0	34.0	40.0	27.0	41.0
36	35.03591510384503	38.0	34.0	40.0	26.0	41.0
37	35.017533215602576	38.0	34.0	40.0	26.0	41.0
38	34.85069139734853	37.0	34.0	40.0	26.0	41.0
39	34.573843548101685	37.0	33.0	40.0	25.0	41.0
40	34.428359389389065	37.0	33.0	40.0	24.0	41.0
41	34.35586107770591	37.0	33.0	40.0	24.0	41.0
42	34.48925074845704	37.0	33.0	40.0	24.0	41.0
43	34.480673737734875	37.0	33.0	40.0	24.0	41.0
44	34.38839232511024	37.0	33.0	40.0	24.0	41.0
45	34.05818282936624	37.0	33.0	40.0	23.0	41.0
46	33.838604132132915	36.0	33.0	39.0	23.0	40.0
47	33.72222402790869	36.0	33.0	39.0	22.0	40.0
48	33.58196552583396	36.0	33.0	39.0	22.0	40.0
49	33.454646572987656	36.0	33.0	39.0	21.0	40.0
50	33.263037959140924	36.0	32.0	39.0	20.0	40.0
51	30.770435856599605	34.0	27.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	3.0
11	9.0
12	7.0
13	14.0
14	23.0
15	32.0
16	62.0
17	167.0
18	284.0
19	508.0
20	859.0
21	1260.0
22	1716.0
23	2349.0
24	2692.0
25	3062.0
26	3522.0
27	4149.0
28	5122.0
29	6456.0
30	8399.0
31	11022.0
32	14433.0
33	19377.0
34	27469.0
35	29611.0
36	30970.0
37	38459.0
38	42441.0
39	22420.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.0283348320531	42.45096658396623	17.341812836986236	22.178885746994435
2	20.252940560412853	42.64598072249127	17.007399703145147	20.093679013950734
3	18.539705239740993	28.597378865523304	29.793104444516672	23.06981145021903
4	26.72018721357298	29.311347294901104	17.576552077803417	26.391913413722495
5	15.727167997457595	40.40692950238892	19.378988309985807	24.486914190167678
6	20.791035127824546	34.24737182334608	26.335214858632806	18.626378190196565
7	66.72661545739844	17.864378500774638	9.484548740894825	5.9244573009320955
8	70.12636193901835	7.400425419731819	6.912890073419212	15.560322567830612
9	67.94545382317996	11.059829615424896	12.485238513125536	8.50947804826961
10	36.32246671216996	28.701386406069997	16.1128626269849	18.863284254775138
11	29.03471612803039	25.00659075560756	26.539618566790534	19.419074549571512
12	26.54936927371679	23.86142439771328	33.8768449601485	15.712361368421432
13	23.962903977205013	32.431212374008226	22.27964305189904	21.32624059688772
14	15.490984207466152	32.123523399890935	34.47741627934692	17.908076113295994
15	14.460298371631945	31.777192735362203	29.209145440822237	24.553363452183614
16	16.514447297429065	27.68875743491403	32.84977049724994	22.947024770406966
17	15.517347229896389	29.82488452635038	27.0697681137438	27.588000130009426
18	18.477228487954264	29.640704506632286	25.52121139893753	26.360855606475912
19	20.5960209892995	32.147358461266215	27.569943265331183	19.686677284103098
20	22.294088543641635	33.59335218470006	24.524472468698423	19.588086802959882
21	18.689938353863987	29.38176906714626	25.845151551265243	26.08314102772451
22	19.029768547108556	32.36729107304724	25.591994308476252	23.01094607136795
23	22.88852052884945	29.029660205920482	20.852067330437013	27.22975193479305
24	16.572229264399446	37.10757918838004	24.612951105621825	21.70724044159868
25	18.519481551301357	28.349999819431353	25.547213284074203	27.583305345193082
26	23.304189553742646	31.867477058753423	19.483718125119626	25.344615262384302
27	17.279697222493077	29.245620307472294	27.770374463259696	25.704308006774934
28	20.443621051415118	33.10762252485527	23.690606457857083	22.758149965872526
29	18.54151092620882	31.148813844559285	25.00225710808478	25.30741812114712
30	21.642235728757	28.676829070107583	25.80939895920232	23.871536241933093
31	24.969393614370375	36.709967028165096	17.695366247386268	20.625273110078258
32	26.45511244009635	26.38649635431902	19.679093400938235	27.47929780464639
33	23.021780190174898	30.42906721848445	20.502486430266195	26.046666161074455
34	18.81380844555675	32.01157083888582	20.70580672654323	28.4688139890142
35	19.1897523681578	27.84007396091772	24.896805018363832	28.073368652560642
36	20.84665027103354	29.9440598332268	25.48112515935183	23.728164736387832
37	21.93367352466387	28.340971387092228	23.401335485711602	26.324019602532296
38	23.21426636764499	29.56883818521287	22.212110378002407	25.004785069139736
39	23.476813180066667	27.72703798803191	25.345337536971428	23.450811294929995
40	21.64295800334413	27.93216397077677	21.57325850568611	28.85161952019299
41	19.01026713325605	25.3579773422462	30.5077951484816	25.123960376016154
42	25.59488340682477	24.256508596873275	21.153255833270133	28.995352163031818
43	21.96581474379115	24.28359389389064	26.309574110789697	27.441017251528514
44	21.377522092573933	23.155762126087474	26.183898332629113	29.282817448709476
45	27.239502641719305	27.65986645142884	22.97230438095651	22.12832652589535
46	19.222254724578644	23.64907566909712	34.29215284774813	22.83651675857611
47	22.52304958776178	25.916295598097527	23.24315735113018	28.31749746301051
48	20.179268552525613	21.847722848795424	33.69483176419179	24.278176834487166
49	22.50318703661571	21.14856104845379	29.81802291777265	26.530228997157852
50	20.782367832778988	23.17743036370137	25.412870210868064	30.62733159265158
51	18.887480453443985	22.520521626706824	32.53305309079353	26.058944829055662
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2455.0
1	1950.0
2	1445.0
3	1081.0
4	717.0
5	717.5
6	718.0
7	790.5
8	863.0
9	944.0
10	1025.0
11	1081.0
12	1137.0
13	1134.5
14	1132.0
15	1114.0
16	1096.0
17	1043.5
18	991.0
19	945.0
20	899.0
21	834.0
22	769.0
23	807.0
24	845.0
25	962.5
26	1356.0
27	1632.0
28	2012.0
29	2392.0
30	2748.5
31	3105.0
32	3367.0
33	3629.0
34	3993.5
35	4358.0
36	5135.5
37	5913.0
38	6722.0
39	7531.0
40	8905.5
41	10280.0
42	12395.0
43	14510.0
44	27691.0
45	40872.0
46	38596.0
47	36320.0
48	31361.5
49	26403.0
50	23273.0
51	20143.0
52	18421.5
53	16700.0
54	15197.0
55	13694.0
56	12274.5
57	10855.0
58	10062.0
59	9269.0
60	8906.0
61	8543.0
62	7521.5
63	6500.0
64	5850.0
65	5200.0
66	4486.5
67	3773.0
68	3190.5
69	2608.0
70	2517.0
71	2426.0
72	2193.0
73	1960.0
74	1574.5
75	1023.5
76	858.0
77	648.5
78	439.0
79	377.0
80	315.0
81	232.0
82	149.0
83	104.5
84	60.0
85	52.0
86	44.0
87	35.0
88	26.0
89	22.0
90	18.0
91	15.0
92	12.0
93	6.5
94	1.0
95	0.5
96	0.0
97	2.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	276903.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.57058970108666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.81133849384186	27.59125036565151
2	7.932976067358216	5.484953214663619
3	2.668003802479969	2.7670339432942224
4	1.3517607362604074	1.8692466314918943
5	0.8952542125001305	1.5474733029255732
6	0.7061748514003364	1.4647728626992125
7	0.5547024350496724	1.3423473201807132
8	0.4847117323221244	1.3405416337128886
9	0.4241227657818588	1.3195956706861247
>10	4.958893520114492	35.887657410717836
>50	0.15356169105894887	3.278043213688548
>100	0.04805331829055543	3.092418644796192
>500	0.0010446373541425095	0.28529846191626673
>1k	0.006267824124855057	4.168968916913142
>5k	0.0031339120624275284	8.56039840666226
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT	9365	3.382050754235238	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCC	7341	2.6511088720598908	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGT	6998	2.527238780367132	No Hit
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT	2452	0.8855086438211215	TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2058	0.7432205501565531	No Hit
AATGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTT	1937	0.6995229376352008	No Hit
AACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTG	1806	0.6522139521781996	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT	1789	0.6460746181875964	No Hit
AATGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC	1502	0.5424282149344716	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTAT	790	0.28529846191626673	No Hit
AAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT	465	0.16792884150767598	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	399	0.14409378013239293	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	389	0.14048240719674399	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTT	380	0.13723217155465994	No Hit
AATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCC	357	0.12892601380266736	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGC	313	0.11303597288581199	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGG	299	0.10798005077590347	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6113729356489454E-4	0.0	0.0	1.1393881611972423	0.0
2	3.6113729356489454E-4	0.0	0.0	1.3445141439421024	0.0
3	3.6113729356489454E-4	0.0	0.0	2.4452606147279012	0.0
4	3.6113729356489454E-4	0.0	0.0	7.599773205779641	0.0
5	3.6113729356489454E-4	0.0	0.0	8.51308942120526	0.0
6	3.6113729356489454E-4	0.0	0.0	10.436506646731887	0.0
7	3.6113729356489454E-4	0.0	0.0	12.09448796148832	0.0
8	3.6113729356489454E-4	0.0	0.0	12.935215580907393	0.0
9	3.6113729356489454E-4	0.0	0.0	16.83585948870182	0.0
10	3.6113729356489454E-4	0.0	0.0	18.250434267595512	0.0
11	3.6113729356489454E-4	0.0	0.0	22.39123447561059	0.0
12	3.6113729356489454E-4	0.0	0.0	23.236295742552446	0.0
13	3.6113729356489454E-4	0.0	0.0	23.774751447257703	0.0
14	3.6113729356489454E-4	0.0	0.0	24.437438380949285	0.0
15	3.6113729356489454E-4	0.0	0.0	24.74909986529579	0.0
16	3.6113729356489454E-4	0.0	0.0	25.187881676977135	0.0
17	3.6113729356489454E-4	0.0	0.0	25.621607566548576	0.0
18	3.6113729356489454E-4	0.0	0.0	26.399858434180924	0.0
19	3.6113729356489454E-4	0.0	0.0	26.668544580593203	0.0
20	3.6113729356489454E-4	0.0	0.0	26.996818380443692	0.0
21	3.6113729356489454E-4	0.0	0.0	27.31389692419367	0.0
22	3.6113729356489454E-4	0.0	0.0	27.600278797990633	0.0
23	3.6113729356489454E-4	0.0	0.0	27.91446824339209	0.0
24	3.6113729356489454E-4	0.0	0.0	28.119233088843387	0.0
25	3.6113729356489454E-4	0.0	0.0	28.320386561359033	0.0
26	3.6113729356489454E-4	0.0	0.0	28.49228791309592	0.0
27	3.6113729356489454E-4	0.0	0.0	28.689468875382353	0.0
28	3.6113729356489454E-4	0.0	0.0	28.86678728652272	0.0
29	3.6113729356489454E-4	0.0	0.0	29.08310852536809	0.0
30	3.6113729356489454E-4	0.0	0.0	29.43882875952951	0.0
31	3.6113729356489454E-4	0.0	0.0	29.64395474227437	0.0
32	3.6113729356489454E-4	0.0	0.0	29.82488452635038	0.0
33	3.6113729356489454E-4	0.0	0.0	30.013398193591257	0.0
34	3.6113729356489454E-4	0.0	0.0	30.205884371061348	0.0
35	3.6113729356489454E-4	0.0	0.0	30.414621726741856	0.0
36	3.6113729356489454E-4	0.0	0.0	30.60024629563421	0.0
37	3.6113729356489454E-4	0.0	0.0	30.77648129489388	0.0
38	3.6113729356489454E-4	0.0	0.0	30.953077431447113	0.0
39	3.6113729356489454E-4	0.0	0.0	31.16073137524693	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACTAG	30	2.1595133E-6	45.000004	1
TGCGGGT	35	1.2074634E-7	45.000004	4
CTAACAG	30	2.1595133E-6	45.000004	1
CATACGG	35	1.2074634E-7	45.000004	2
ACGCGAG	20	7.0240034E-4	45.0	1
AGCGTAT	20	7.0240034E-4	45.0	16
ACCGGGT	40	6.7830115E-9	45.0	4
GCGAAGA	25	3.8831124E-5	45.0	40
TAGCGTA	20	7.0240034E-4	45.0	15
TAGCGCG	20	7.0240034E-4	45.0	1
ATTACCG	20	7.0240034E-4	45.0	1
TCGGGTG	20	7.0240034E-4	45.0	5
CAATTGG	20	7.0240034E-4	45.0	2
TAAGTCG	20	7.0240034E-4	45.0	23
CGCGAAG	20	7.0240034E-4	45.0	1
GCGTTGG	25	3.8831124E-5	45.0	2
GTAGCGT	20	7.0240034E-4	45.0	14
CTAACCG	20	7.0240034E-4	45.0	1
CAAGGTC	20	7.0240034E-4	45.0	28
CGGGCTA	20	7.0240034E-4	45.0	6
>>END_MODULE