SRR3548625_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548625_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	316965
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT	9998	3.1542914832867983	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCC	7817	2.466202893063903	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGT	7775	2.452952218699225	No Hit
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT	2674	0.8436262678844668	TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2503	0.7896770936854227	No Hit
AATGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTT	2135	0.6735759468711056	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT	1907	0.6016437146057135	No Hit
AACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTG	1900	0.5994352688782673	No Hit
AATGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC	1617	0.5101509630400833	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTAT	801	0.25270928966920636	No Hit
AATCTGACTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT	632	0.1993910999637184	No Hit
AATCTGTCACTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT	547	0.17257425898758538	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	515	0.16247850709068826	No Hit
AAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT	504	0.15900809237612987	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	461	0.14544192576467432	No Hit
AATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCC	370	0.11673213130787312	No Hit
AATGATACATGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGT	363	0.11452368558042686	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTT	351	0.11073777861909044	No Hit
AATGATACAGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCC	337	0.10632088716419795	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCCGT	30	2.1605101E-6	45.000004	44
GTAGCCG	30	2.1605101E-6	45.000004	43
TCGTCGC	30	2.1605101E-6	45.000004	11
CATACGG	45	3.8380676E-10	45.000004	2
AACGTAT	20	7.025547E-4	45.0	10
CGGCGAA	20	7.025547E-4	45.0	30
GTCGCCC	25	3.8843922E-5	45.0	13
GGTACCC	20	7.025547E-4	45.0	8
GTTCTTA	20	7.025547E-4	45.0	32
ATTAGGT	20	7.025547E-4	45.0	40
CGAAACG	20	7.025547E-4	45.0	1
GACACGC	25	3.8843922E-5	45.0	13
TAGCGCG	20	7.025547E-4	45.0	1
ATTCGGT	20	7.025547E-4	45.0	34
CCTAAGT	25	3.8843922E-5	45.0	12
CATTACG	20	7.025547E-4	45.0	1
TACATAC	20	7.025547E-4	45.0	35
CTAACGG	50	2.1827873E-11	45.0	2
TACGCCA	20	7.025547E-4	45.0	38
CTACGGA	25	3.8843922E-5	45.0	3

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content