##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548624_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 170189 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.829189900639875 31.0 31.0 33.0 28.0 34.0 2 30.976467339252242 31.0 31.0 34.0 28.0 34.0 3 31.243899429457837 31.0 31.0 34.0 28.0 34.0 4 35.286634271310135 37.0 35.0 37.0 33.0 37.0 5 34.867335726750845 35.0 35.0 37.0 32.0 37.0 6 34.85945037575871 35.0 35.0 37.0 32.0 37.0 7 33.700873734495175 35.0 35.0 37.0 30.0 37.0 8 34.52384114131936 35.0 35.0 37.0 31.0 37.0 9 35.769303539006636 38.0 35.0 39.0 31.0 39.0 10 35.47334434070357 37.0 35.0 39.0 30.0 39.0 11 35.88230731715916 37.0 35.0 39.0 31.0 39.0 12 35.97516878294132 37.0 35.0 39.0 31.0 39.0 13 35.926129185787566 37.0 35.0 39.0 31.0 39.0 14 36.91835547538325 38.0 36.0 40.0 31.0 41.0 15 36.94120066514287 38.0 36.0 40.0 32.0 41.0 16 36.79984017768481 38.0 35.0 40.0 31.0 41.0 17 36.7637802678199 38.0 35.0 40.0 31.0 41.0 18 36.49658908625117 38.0 35.0 40.0 31.0 41.0 19 36.40080733772453 38.0 35.0 40.0 30.0 41.0 20 36.39071855407811 38.0 35.0 40.0 30.0 41.0 21 36.43734318904277 38.0 35.0 40.0 30.0 41.0 22 36.39547796861137 38.0 35.0 40.0 30.0 41.0 23 36.28257407940584 38.0 35.0 40.0 30.0 41.0 24 35.96716003972055 38.0 34.0 40.0 30.0 41.0 25 35.994124179588574 38.0 34.0 40.0 30.0 41.0 26 35.97902919695162 38.0 34.0 40.0 30.0 41.0 27 35.80866565994277 38.0 34.0 40.0 29.0 41.0 28 35.58864556463696 38.0 34.0 40.0 27.0 41.0 29 35.407452890609854 38.0 34.0 40.0 27.0 41.0 30 35.35628037064675 38.0 34.0 40.0 27.0 41.0 31 35.13575495478556 38.0 34.0 40.0 26.0 41.0 32 34.97091468896345 38.0 34.0 40.0 25.0 41.0 33 34.87345833161955 38.0 34.0 40.0 25.0 41.0 34 34.84763410091134 38.0 34.0 40.0 25.0 41.0 35 34.716479913507925 38.0 33.0 40.0 25.0 41.0 36 34.630334510456024 38.0 33.0 40.0 24.0 41.0 37 34.55370793647063 38.0 33.0 40.0 24.0 41.0 38 34.40966219908455 37.0 33.0 40.0 23.0 41.0 39 34.29917327206811 37.0 33.0 40.0 23.0 41.0 40 34.08077490319586 37.0 33.0 40.0 22.0 41.0 41 34.01728666365041 37.0 33.0 40.0 22.0 41.0 42 33.90877788811263 37.0 33.0 40.0 21.0 41.0 43 33.88841817038704 37.0 33.0 40.0 21.0 41.0 44 33.81889546327906 37.0 33.0 40.0 21.0 41.0 45 33.597629694046034 37.0 33.0 40.0 20.0 41.0 46 33.47886761189031 37.0 33.0 40.0 20.0 41.0 47 33.293156431966814 37.0 32.0 40.0 19.0 41.0 48 33.170522184159964 36.0 32.0 40.0 18.0 40.0 49 32.98558073671036 36.0 32.0 39.0 17.0 40.0 50 32.879868851688414 36.0 32.0 39.0 15.0 40.0 51 30.68183020054175 34.0 27.0 38.0 10.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 1.0 10 3.0 11 4.0 12 3.0 13 3.0 14 10.0 15 18.0 16 40.0 17 93.0 18 217.0 19 430.0 20 683.0 21 1161.0 22 1566.0 23 1843.0 24 2171.0 25 2308.0 26 2511.0 27 2704.0 28 3303.0 29 3960.0 30 4864.0 31 6154.0 32 7903.0 33 10495.0 34 14448.0 35 17450.0 36 19463.0 37 24850.0 38 27980.0 39 13548.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.242201317358933 36.43831269941066 19.488921140614256 23.830564842616152 2 21.723495643079165 37.96896391658685 18.48885650658973 21.81868393374425 3 18.60108467644795 36.44947675819236 19.911392628195713 25.03804593716398 4 19.18102815105559 36.57169382275 17.150932198908272 27.096345827286132 5 14.965127005858193 40.027263806709016 17.574578850572014 27.433030336860785 6 17.857793394402695 41.44686201810928 19.294431484996093 21.400913102491938 7 71.7302528365523 15.342354676271675 7.3718042881737365 5.555588199002286 8 75.4179177267626 11.262772564619334 5.777106628513006 7.54220308010506 9 68.90163289049234 15.624394056020071 10.317940642462203 5.1560324110253895 10 28.542972812578952 42.92639359770608 12.036030530762858 16.494603058952105 11 20.124684909130437 32.43394108902456 30.528412529599446 16.912961472245563 12 22.439170569190722 28.145767352766633 31.416248993765755 17.998813084276893 13 23.446873769750102 31.299907749619543 25.96525039808683 19.287968082543525 14 16.616232541468605 36.41892249205295 28.807972313134222 18.156872653344223 15 15.465746904911597 35.12800474766289 29.099413005540896 20.30683534188461 16 16.7572522313428 35.36773822044903 27.30670019801515 20.56830935019302 17 16.221377409820846 32.928685167666536 26.584561869451022 24.265375553061595 18 20.21164705121953 30.98026311923802 25.49400960109055 23.314080228451896 19 19.00710386687741 36.716239004871056 25.687911674667575 18.588745453583957 20 20.30624775984347 34.16554536427149 24.41403380947065 21.1141730664144 21 19.084077114267078 34.097973429540104 25.240174159316997 21.577775296875824 22 17.638625293056542 33.053840142429884 24.802425538665837 24.505109025847734 23 18.69568538507189 34.78544441767682 23.13310495978001 23.385765237471283 24 15.573862000481817 36.10045302575372 24.067360405196574 24.258324568567886 25 17.665066484907953 34.97699616308927 23.557339193484893 23.800598158517882 26 20.51660213057248 34.87534446997162 22.859291728607605 21.748761670848292 27 15.908783763933041 33.53095675983759 24.21660624364677 26.343653232582597 28 17.79080904171245 35.79608552844191 25.244874815646135 21.16823061419951 29 18.03406800674544 34.287762428829126 25.054498234315965 22.623671330109467 30 21.081268472110416 32.08550493862706 22.658926252578016 24.17430033668451 31 20.479584461980505 35.89068623706585 20.583586483262724 23.04614281769092 32 20.754572857235193 31.79171391805581 21.064228592917285 26.389484631791717 33 20.461369418705086 33.479837122258196 22.020224573856126 24.038568885180595 34 16.59155409574062 32.54910716908848 22.30637702789252 28.552961707278378 35 18.458302240450323 33.873517089823665 23.261785426790215 24.406395242935794 36 19.407834818936596 30.37211570665554 25.98229027727997 24.237759197127897 37 17.886584914418677 33.27183307969375 20.88619123445111 27.955390771436463 38 21.02721092432531 32.443929983723976 23.586718295542013 22.9421407964087 39 21.240503205260033 27.94833978694275 23.644888917615123 27.166268090182093 40 22.98738461357667 30.355663409503553 22.069581465312094 24.587370511607684 41 19.41841129567716 28.17455887278261 26.56282133392875 25.844208497611483 42 22.734136753844254 30.432049074852074 23.557339193484893 23.27647497781878 43 18.80203773451868 28.243305971596282 26.91830846881996 26.036347825065075 44 21.066578921081856 27.57816310102298 24.248923255909606 27.106334721985558 45 23.94808125084465 28.634635610997183 25.02276880409427 22.394514334063896 46 19.70808924196041 26.477621937963086 28.844402399685055 24.969886420391447 47 21.833961066813952 28.122264071120927 24.64142805939279 25.40234680267232 48 20.550094306917604 25.652069170157883 30.266350939249893 23.531485583674623 49 21.574249804628973 25.451116112087153 27.141589644454108 25.833044438829774 50 19.721016046865543 24.13434475788682 26.286070192550635 29.858569002697 51 19.77918666893865 24.07499897173143 30.169987484502524 25.975826874827394 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3150.0 1 2450.0 2 1750.0 3 1239.5 4 729.0 5 738.0 6 747.0 7 834.0 8 921.0 9 1005.0 10 1089.0 11 1195.0 12 1301.0 13 1303.5 14 1306.0 15 1293.0 16 1280.0 17 1200.0 18 1120.0 19 1043.0 20 966.0 21 933.0 22 900.0 23 761.5 24 623.0 25 706.0 26 940.0 27 1091.0 28 1252.0 29 1413.0 30 1752.0 31 2091.0 32 2441.0 33 2791.0 34 2934.0 35 3077.0 36 3630.0 37 4183.0 38 4770.0 39 5357.0 40 5655.5 41 5954.0 42 7679.5 43 9405.0 44 12317.5 45 15230.0 46 16319.5 47 17409.0 48 18728.0 49 20047.0 50 18679.0 51 17311.0 52 15385.0 53 13459.0 54 11422.5 55 9386.0 56 8212.0 57 7038.0 58 5965.5 59 4893.0 60 4335.5 61 3778.0 62 3187.5 63 2597.0 64 2041.5 65 1486.0 66 1436.5 67 1387.0 68 1249.5 69 1112.0 70 940.5 71 769.0 72 842.0 73 915.0 74 687.5 75 426.5 76 393.0 77 327.5 78 262.0 79 177.0 80 92.0 81 67.5 82 43.0 83 38.0 84 33.0 85 31.5 86 30.0 87 22.0 88 14.0 89 9.5 90 5.0 91 4.0 92 3.0 93 2.5 94 2.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 170189.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.90256127011734 #Duplication Level Percentage of deduplicated Percentage of total 1 81.42264760783233 38.189307182015284 2 8.105433271112336 7.603311612383878 3 2.748581235984616 3.867464994799899 4 1.4945567067136039 2.803941500331984 5 1.0548338198263658 2.4737203932099017 6 0.7040577277225862 1.9813266427324914 7 0.5336807687007503 1.7521696466869185 8 0.42970071282713 1.612325120895005 9 0.3457650050737256 1.4595537901979565 >10 3.0241910226375857 27.60401671083325 >50 0.0964634253285394 2.9490742644941803 >100 0.031319293937837465 2.780438218686284 >500 0.005011087030053995 1.815628507130308 >1k 0.0037583152725404957 3.1077214156026534 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2700 1.5864715110847354 No Hit CTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTGCT 1512 0.8884240462074517 No Hit AATCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCT 1077 0.6328258583104666 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCC 1000 0.5875820411424945 No Hit AATGATACCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGT 905 0.5317617472339575 No Hit AAACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCT 668 0.39250480348318634 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 517 0.30377991527066966 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 490 0.2879152001598223 No Hit AAAAACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTT 444 0.26088642626726755 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 316 0.18567592500102825 No Hit AACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTG 273 0.160409897231901 No Hit AAAAAACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCT 266 0.15629682294390354 No Hit AATGACTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTT 247 0.14513276416219614 No Hit AATGATCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCT 214 0.12574255680449384 No Hit CCACCAGGGAGTCATCTCCCTTTCACATGATACAGATGCAAATTGCAAATG 177 0.10400202128222154 No Hit CCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCCGTCTTCTGC 175 0.10282685719993653 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.1117052218415997 0.0 2 0.0 0.0 0.0 1.4742433412265188 0.0 3 0.0 0.0 0.0 2.020107057447896 0.0 4 0.0 0.0 0.0 4.658350422177697 0.0 5 0.0 0.0 0.0 5.08317223792372 0.0 6 0.0 0.0 0.0 7.150285858663016 0.0 7 0.0 0.0 0.0 8.770837128134016 0.0 8 0.0 0.0 0.0 10.117575166432614 0.0 9 0.0 0.0 0.0 12.131218821427941 0.0 10 0.0 0.0 0.0 14.633143152612684 0.0 11 0.0 0.0 0.0 17.26316036876649 0.0 12 0.0 0.0 0.0 18.581106887049103 0.0 13 0.0 0.0 0.0 19.31264652827151 0.0 14 0.0 0.0 0.0 19.8614481546986 0.0 15 0.0 0.0 0.0 20.253365376140643 0.0 16 0.0 0.0 0.0 21.101246261509264 0.0 17 0.0 0.0 0.0 22.02845072243212 0.0 18 0.0 0.0 0.0 23.577316982883737 0.0 19 0.0 0.0 0.0 24.076174135813712 0.0 20 0.0 0.0 0.0 24.886449770549213 0.0 21 0.0 0.0 0.0 25.447590619840295 0.0 22 0.0 0.0 0.0 26.052800122217064 0.0 23 0.0 0.0 0.0 26.660947534799547 0.0 24 0.0 0.0 0.0 27.101634065656416 0.0 25 0.0 0.0 0.0 27.467110095247047 0.0 26 0.0 0.0 0.0 27.824947558302828 0.0 27 0.0 0.0 0.0 28.25388244833685 0.0 28 0.0 0.0 0.0 28.686930412658867 0.0 29 0.0 0.0 0.0 29.094712349211758 0.0 30 0.0 0.0 0.0 29.77513235285477 0.0 31 0.0 0.0 0.0 30.201129332683077 0.0 32 0.0 0.0 0.0 30.60714852311254 0.0 33 0.0 0.0 0.0 31.004353982924865 0.0 34 0.0 0.0 0.0 31.37335550476235 0.0 35 0.0 0.0 0.0 31.776436784986103 0.0 36 0.0 0.0 0.0 32.153664455399586 0.0 37 0.0 0.0 0.0 32.49504962130337 0.0 38 0.0 0.0 0.0 32.87462761988142 0.0 39 0.0 0.0 0.0 33.25361803641834 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGAGAG 20 7.0163346E-4 45.000004 23 GTTGACA 20 7.0163346E-4 45.000004 28 CCTTATC 20 7.0163346E-4 45.000004 26 CGAACAG 20 7.0163346E-4 45.000004 1 GCGACCG 20 7.0163346E-4 45.000004 32 GCGACAG 20 7.0163346E-4 45.000004 1 CAAAAGA 20 7.0163346E-4 45.000004 23 TGCCAGC 20 7.0163346E-4 45.000004 9 GTTTACA 20 7.0163346E-4 45.000004 28 ATTACTC 20 7.0163346E-4 45.000004 32 GGCCGAT 20 7.0163346E-4 45.000004 8 AAGGTTC 20 7.0163346E-4 45.000004 8 CACTGCG 20 7.0163346E-4 45.000004 1 CCAGACG 20 7.0163346E-4 45.000004 1 GAGACTA 20 7.0163346E-4 45.000004 1 GCATTCC 20 7.0163346E-4 45.000004 12 GATATTC 20 7.0163346E-4 45.000004 9 GTCCGAC 20 7.0163346E-4 45.000004 40 ATGGTCA 40 6.7557266E-9 45.000004 13 CGAGAGC 20 7.0163346E-4 45.000004 24 >>END_MODULE