SRR3548621_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548621_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	244745
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2630	1.0745878363194346	No Hit
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	1632	0.6668164824613373	RNA PCR Primer, Index 16 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	987	0.4032768800179779	RNA PCR Primer, Index 14 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGT	894	0.36527814664242375	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC	839	0.3428057774418272	No Hit
AAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	619	0.2529163006394411	RNA PCR Primer, Index 14 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	614	0.25087335798484134	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	483	0.19734826043432963	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	479	0.19571390631064983	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	296	0.12094220515230136	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	256	0.10459866391550389	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	251	0.1025557212609042	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATGC	20	7.0223934E-4	45.000004	39
CGGGTAT	45	3.8198777E-10	45.000004	6
AGACTCG	20	7.0223934E-4	45.000004	10
TCCATTG	20	7.0223934E-4	45.000004	17
ACACGCT	20	7.0223934E-4	45.000004	14
GATCGAT	35	1.2066994E-7	45.000004	10
ACGTTTG	20	7.0223934E-4	45.000004	28
CCGATTC	20	7.0223934E-4	45.000004	2
GAATGAC	20	7.0223934E-4	45.000004	9
TAGGTGT	20	7.0223934E-4	45.000004	6
CGTTGCG	20	7.0223934E-4	45.000004	17
GGCCGAT	20	7.0223934E-4	45.000004	8
ATAGCGG	40	6.7775545E-9	45.000004	2
TAAACCG	20	7.0223934E-4	45.000004	1
AATGGTC	20	7.0223934E-4	45.000004	9
CGTCTAC	20	7.0223934E-4	45.000004	40
CGATTAT	20	7.0223934E-4	45.000004	27
CGCGACG	20	7.0223934E-4	45.000004	1
GTAATTG	45	3.8198777E-10	45.000004	1
CGCTCGT	20	7.0223934E-4	45.000004	13