##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548620_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	212817
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.011963329997133	31.0	31.0	33.0	28.0	34.0
2	31.18266397891146	31.0	31.0	34.0	28.0	34.0
3	31.374147741956705	31.0	31.0	34.0	28.0	34.0
4	35.355563700268306	37.0	35.0	37.0	33.0	37.0
5	34.94959989098615	35.0	35.0	37.0	32.0	37.0
6	34.98538180690452	35.0	35.0	37.0	32.0	37.0
7	33.85409060366418	35.0	35.0	37.0	30.0	37.0
8	34.61460315670271	37.0	35.0	37.0	32.0	37.0
9	35.990550566919	39.0	35.0	39.0	32.0	39.0
10	35.64939361047285	37.0	35.0	39.0	30.0	39.0
11	36.076511744832416	37.0	35.0	39.0	31.0	39.0
12	35.96403012917201	37.0	35.0	39.0	31.0	39.0
13	36.02491342326976	37.0	35.0	39.0	31.0	39.0
14	37.05894265965595	39.0	36.0	40.0	32.0	41.0
15	37.08551948387582	39.0	36.0	40.0	32.0	41.0
16	36.96440603899124	38.0	35.0	40.0	31.0	41.0
17	36.87624578863531	38.0	35.0	40.0	31.0	41.0
18	36.60284657710615	38.0	35.0	40.0	31.0	41.0
19	36.596949491816915	38.0	35.0	40.0	31.0	41.0
20	36.68484190642665	38.0	35.0	40.0	31.0	41.0
21	36.818985325420435	38.0	35.0	40.0	31.0	41.0
22	36.77466085885996	39.0	35.0	40.0	31.0	41.0
23	36.71222223788513	38.0	35.0	40.0	31.0	41.0
24	36.404032572585834	38.0	35.0	40.0	30.0	41.0
25	36.46253823707693	38.0	35.0	40.0	30.0	41.0
26	36.42993745800383	38.0	35.0	40.0	30.0	41.0
27	36.33150077296457	38.0	35.0	40.0	30.0	41.0
28	36.222533914114	38.0	35.0	40.0	30.0	41.0
29	36.0074618099118	38.0	35.0	40.0	30.0	41.0
30	36.05360004135008	38.0	35.0	40.0	30.0	41.0
31	35.84073640733588	38.0	34.0	40.0	29.0	41.0
32	35.8153202046829	38.0	35.0	40.0	29.0	41.0
33	35.83649802412401	38.0	35.0	40.0	29.0	41.0
34	35.637256422184315	38.0	34.0	40.0	28.0	41.0
35	35.70574249237608	38.0	35.0	40.0	29.0	41.0
36	35.53912516387319	38.0	34.0	40.0	28.0	41.0
37	35.545233698435744	38.0	34.0	40.0	28.0	41.0
38	35.42562859170085	38.0	34.0	40.0	28.0	41.0
39	35.27010060286537	38.0	34.0	40.0	27.0	41.0
40	35.05850096561835	38.0	34.0	40.0	26.0	41.0
41	34.974024631490906	38.0	34.0	40.0	26.0	41.0
42	35.02598946512732	38.0	34.0	40.0	26.0	41.0
43	35.07420459831686	38.0	34.0	40.0	26.0	41.0
44	34.99720417071944	38.0	34.0	40.0	26.0	41.0
45	34.75708237593801	37.0	34.0	40.0	26.0	41.0
46	34.5920438686759	37.0	33.0	40.0	25.0	41.0
47	34.37376713326473	37.0	33.0	40.0	24.0	41.0
48	34.25960802003599	37.0	33.0	40.0	24.0	41.0
49	34.078706118402195	37.0	33.0	40.0	23.0	41.0
50	33.963113848987625	36.0	33.0	40.0	23.0	40.0
51	31.845045273638853	35.0	29.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	1.0
11	9.0
12	8.0
13	5.0
14	14.0
15	17.0
16	42.0
17	80.0
18	168.0
19	310.0
20	537.0
21	739.0
22	1075.0
23	1388.0
24	1667.0
25	1962.0
26	2337.0
27	2742.0
28	3270.0
29	4353.0
30	5579.0
31	7441.0
32	9768.0
33	12905.0
34	18254.0
35	20936.0
36	24568.0
37	32286.0
38	38745.0
39	21607.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.89063843583924	36.011690795378186	18.766827838001664	23.330842930780907
2	25.33867125276646	34.78951399559246	18.93645714393117	20.93535760770991
3	20.055728630701495	31.501712739113884	23.386289629117975	25.056269001066646
4	23.711921510029743	32.08860194439354	17.432347979719665	26.76712856585705
5	16.697914170390522	37.79726243674143	17.691255867717334	27.813567525150717
6	19.234835562948447	37.72302024744264	22.71529060178463	20.32685358782428
7	74.76188462387873	13.555777968865268	6.7367738479538755	4.945563559302124
8	78.40492065953379	6.328441806810546	5.47418674260045	9.792450791055225
9	73.98610073443382	10.000140966182212	9.604495881438043	6.409262417945935
10	33.43200966088236	32.93204960130065	14.66377216105856	18.972168576758435
11	26.511509888777685	28.46248185060404	25.138969161298203	19.887039099320074
12	25.687797497380377	25.150246455875237	31.954214184017253	17.207741862727133
13	25.234826165202968	28.880211637228232	25.806678977713247	20.078283219855557
14	18.721248772419496	30.215161382784274	32.072625777076084	18.990964067720153
15	17.710521245953096	31.905815794790833	27.12330311958161	23.260359839674464
16	19.413392727084773	29.065817110475194	29.557319199124134	21.9634709633159
17	18.939746354849472	28.341720821175	28.184778471644652	24.533754352330874
18	20.758210105395715	28.595459949158197	25.907704741632482	24.738625203813605
19	19.410573403440516	33.43999774454108	27.081953039465834	20.06747581255257
20	21.482776281969958	33.57673494128758	23.882490590507338	21.05799818623512
21	21.377521532584336	29.22980776911619	26.426930179449947	22.96574051884953
22	21.40430510720478	30.55160067099903	23.80683873938642	24.23725548240977
23	22.733616205472305	30.527636420022837	21.953603330561	24.78514404394386
24	18.639488386736023	34.482207718368365	24.333582373588573	22.544721521307036
25	21.39302781262775	27.265209076342583	25.00928027366235	26.332482837367316
26	24.14844678761565	29.06957620866754	22.1735105748131	24.608466428903704
27	18.18322784364031	26.817406504179647	28.159874446120376	26.83949120605967
28	21.754371126366784	29.349159136723102	26.170841615096542	22.725628121813575
29	22.23788513135699	31.2874441421503	23.804959190290248	22.669711536202463
30	24.953363688051237	25.92368090894994	24.400306366502676	24.722649036496144
31	25.19112664871697	31.390819342439748	21.11814375731262	22.299910251530658
32	22.97043939158996	25.531794922398117	23.763609110174468	27.734156575837453
33	25.114065135773927	26.01248960374406	25.08681167387944	23.786633586602573
34	21.04014246982149	29.611356235639068	24.173350813139926	25.175150481399513
35	22.21298110583271	24.99706320453723	28.703064134914037	24.086891554716022
36	25.35887640555031	29.599139166513954	24.269677704318735	20.772306723617003
37	19.989944412335483	30.200124990014892	21.40242555810861	28.407505039541014
38	24.1183740020769	30.20059487728894	23.924780445171205	21.756250675462958
39	22.28628352058341	26.936287984512514	23.96096176527251	26.81646672963156
40	25.138969161298203	26.6407288891395	25.16669251046674	23.05360943909556
41	20.732836192597397	25.496083489570854	30.211872171865966	23.559208145965783
42	24.04648124914833	26.452773979522316	25.81701649774219	23.683728273587164
43	22.419731506411612	24.344859668165608	27.276956258193657	25.958452567229124
44	22.193245840322906	23.167322159413956	25.700954341053585	28.938477659209553
45	26.792502478655372	25.079293477494748	25.970199749080198	22.158004294769682
46	21.473378536489097	24.259340184289787	30.865955257333766	23.40132602188735
47	23.212901224996124	27.84223064886734	24.4454155448108	24.49945258132574
48	20.812247141910657	25.15917431408205	30.806749460804355	23.221829083202937
49	23.515508629479786	23.557328596869613	28.657015182057826	24.270147591592778
50	21.752961464544658	24.274846464333205	26.75209217308768	27.220099898034462
51	20.934417833161824	21.460221692815892	31.63657038676422	25.968790087258064
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2117.0
1	1652.5
2	1188.0
3	813.5
4	439.0
5	400.5
6	362.0
7	368.0
8	374.0
9	410.0
10	446.0
11	477.0
12	508.0
13	534.5
14	561.0
15	542.5
16	524.0
17	549.0
18	574.0
19	570.5
20	567.0
21	585.0
22	603.0
23	778.5
24	954.0
25	868.0
26	1075.0
27	1368.0
28	1781.0
29	2194.0
30	2603.0
31	3012.0
32	3397.0
33	3782.0
34	4287.5
35	4793.0
36	5196.5
37	5600.0
38	5711.0
39	5822.0
40	6598.0
41	7374.0
42	8818.5
43	10263.0
44	15772.5
45	21282.0
46	21703.0
47	22124.0
48	21666.5
49	21209.0
50	19886.0
51	18563.0
52	17075.5
53	15588.0
54	14335.0
55	13082.0
56	11826.0
57	10570.0
58	9388.5
59	8207.0
60	7527.5
61	6848.0
62	6176.0
63	5504.0
64	4972.5
65	4441.0
66	3735.5
67	3030.0
68	2674.0
69	2318.0
70	2061.0
71	1804.0
72	1621.5
73	1439.0
74	1265.0
75	828.5
76	566.0
77	481.5
78	397.0
79	356.5
80	316.0
81	206.0
82	96.0
83	77.5
84	59.0
85	41.5
86	24.0
87	27.0
88	30.0
89	22.5
90	15.0
91	9.0
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	212817.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.797032191977145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.69257568801144	27.382680894853323
2	8.234531558052232	5.730745194227905
3	2.783104221243957	2.9053130154076037
4	1.4691981526993814	2.044949416634949
5	0.8993437221487023	1.5647246225630473
6	0.7386501741972075	1.5421700334089852
7	0.5577011370081292	1.358444109258189
8	0.5198908904313069	1.4472528040523078
9	0.48343100980365683	1.5139767969664077
>10	5.321792205687741	39.466771921416054
>50	0.2457666027493451	5.288111382079439
>100	0.04321171037351122	2.8268418406424303
>500	0.0040510978475166775	0.8716408933496854
>1k	0.006751829745861129	6.0563770751396735
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCC	3288	1.544989357053243	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGT	3177	1.4928318696344747	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT	2776	1.3044070727432489	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1841	0.8650624715130841	No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT	1807	0.8490863041956235	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTT	737	0.34630692096965937	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT	575	0.2701851825747003	No Hit
AACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	543	0.2551487898053257	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	424	0.1992322041942138	No Hit
AAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT	393	0.18466569869888214	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	390	0.18325603687675326	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	340	0.15976167317460543	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTT	335	0.15741223680439062	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTAT	316	0.14848437859757443	No Hit
AATGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC	289	0.1357974221984146	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	249	0.11700193123669632	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0487883956638802	0.0
2	0.0	0.0	0.0	1.2743342872044996	0.0
3	0.0	0.0	0.0	1.817523975998158	0.0
4	0.0	0.0	0.0	4.527833772677934	0.0
5	0.0	0.0	0.0	4.913141337393159	0.0
6	0.0	0.0	0.0	6.488203479985152	0.0
7	0.0	0.0	0.0	7.617812486784421	0.0
8	0.0	0.0	0.0	8.31371553964204	0.0
9	0.0	0.0	0.0	10.864263663147211	0.0
10	0.0	0.0	0.0	12.304938045362917	0.0
11	0.0	0.0	0.0	15.253480689982473	0.0
12	0.0	0.0	0.0	15.989324161133744	0.0
13	0.0	0.0	0.0	16.383559584055785	0.0
14	0.0	0.0	0.0	16.833711592588937	0.0
15	0.0	0.0	0.0	17.10577632425981	0.0
16	0.0	0.0	0.0	17.633929620284093	0.0
17	0.0	0.0	0.0	18.23068645831865	0.0
18	0.0	0.0	0.0	19.16952123185648	0.0
19	0.0	0.0	0.0	19.46695987632567	0.0
20	0.0	0.0	0.0	19.84192992101195	0.0
21	0.0	0.0	0.0	20.182598194693092	0.0
22	0.0	0.0	0.0	20.519507370181895	0.0
23	0.0	0.0	0.0	20.936297382257997	0.0
24	0.0	0.0	0.0	21.21775985940973	0.0
25	4.698872740429571E-4	0.0	0.0	21.477607521955484	0.0
26	4.698872740429571E-4	0.0	0.0	21.701743751673973	0.0
27	4.698872740429571E-4	0.0	0.0	22.008580141624023	0.0
28	4.698872740429571E-4	0.0	0.0	22.270307353265952	0.0
29	4.698872740429571E-4	0.0	0.0	22.550830055869596	0.0
30	4.698872740429571E-4	0.0	0.0	22.988764995277634	0.0
31	4.698872740429571E-4	0.0	0.0	23.24391378508296	0.0
32	4.698872740429571E-4	0.0	0.0	23.534304120441504	0.0
33	4.698872740429571E-4	0.0	0.0	23.83879107402134	0.0
34	4.698872740429571E-4	0.0	0.0	24.100048398389227	0.0
35	4.698872740429571E-4	0.0	0.0	24.408294450161407	0.0
36	4.698872740429571E-4	0.0	0.0	24.641828425360757	0.0
37	4.698872740429571E-4	0.0	0.0	24.908724397017156	0.0
38	4.698872740429571E-4	0.0	0.0	25.176560143221643	0.0
39	4.698872740429571E-4	0.0	0.0	25.469769802224448	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGAT	30	2.1571414E-6	45.000004	8
CTAACGG	35	1.205699E-7	45.000004	2
CGGGCAC	65	0.0	45.000004	6
TTACGGG	35	1.205699E-7	45.000004	3
CGACTCG	35	1.205699E-7	45.000004	1
GATTAGG	30	2.1571414E-6	45.000004	2
TTTAGCG	25	3.8800626E-5	45.0	1
ACCGGGT	40	6.7702786E-9	45.0	4
CGAACAG	25	3.8800626E-5	45.0	1
TACCAGG	25	3.8800626E-5	45.0	2
TTTACGG	25	3.8800626E-5	45.0	2
TACCAAT	20	7.020321E-4	45.0	28
ACTTGCA	20	7.020321E-4	45.0	26
GGGTCGT	20	7.020321E-4	45.0	7
TTTCGCT	25	3.8800626E-5	45.0	28
AATGGTC	20	7.020321E-4	45.0	9
ATTCGCG	20	7.020321E-4	45.0	1
AATGGCC	20	7.020321E-4	45.0	11
AGGGTCG	20	7.020321E-4	45.0	6
GTAGGGT	45	3.8198777E-10	45.0	4
>>END_MODULE