FastQCFastQC Report
Sat 18 Jun 2016
SRR3548619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245176
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGT35411.4442686070414723No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCC34831.420612131693151No Hit
AATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT30971.2631742095474272No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22440.915260873821255No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT18880.7700590596143179No Hit
AATGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTT7760.3165073253499527No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT6150.25084021274513No Hit
AACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG5380.2194342023689105No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5080.20719809443012369No Hit
AAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT4860.19822494860834666No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4650.18965967305119588No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTAT3960.16151662479198617No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG3850.15703005188109764No Hit
AATGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC3290.1341893170620289No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTT3050.12440043071099945No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2860.11665089568310112No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCC207.022418E-445.00000417
CGGGTCA207.022418E-445.0000046
CGGGTAC351.2066994E-745.0000046
AGACTAC207.022418E-445.00000419
CGAACAG406.7775545E-945.0000041
CATATGC207.022418E-445.00000433
CAAGTGC207.022418E-445.00000426
TTGTCTA207.022418E-445.00000444
TATGCAC207.022418E-445.00000423
ATCCGCG207.022418E-445.0000041
TGTTGCC207.022418E-445.00000441
GCCGATG207.022418E-445.0000049
AGTGATA207.022418E-445.00000445
ACTACGG207.022418E-445.0000042
CGGTGAC207.022418E-445.00000427
TCCAATT207.022418E-445.00000411
CCGGTCT207.022418E-445.00000422
CTTTACG207.022418E-445.0000041
CGTTTGG207.022418E-445.0000042
TACACCG207.022418E-445.0000041