##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548618_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	219738
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.973896185457225	31.0	31.0	33.0	28.0	34.0
2	31.14628785189635	31.0	31.0	34.0	28.0	34.0
3	31.33809809864475	31.0	31.0	34.0	28.0	34.0
4	35.31247212589538	37.0	35.0	37.0	33.0	37.0
5	34.89447887939273	35.0	35.0	37.0	32.0	37.0
6	34.907744677752596	35.0	35.0	37.0	32.0	37.0
7	33.794473418343664	35.0	35.0	37.0	30.0	37.0
8	34.55887010894793	37.0	35.0	37.0	31.0	37.0
9	35.930153182426345	38.0	35.0	39.0	32.0	39.0
10	35.611987912878064	37.0	35.0	39.0	30.0	39.0
11	36.03440460912541	37.0	35.0	39.0	31.0	39.0
12	35.873158033658264	37.0	35.0	39.0	31.0	39.0
13	35.91005652185785	37.0	35.0	39.0	31.0	39.0
14	36.796557718737766	38.0	35.0	40.0	31.0	41.0
15	36.894123911203344	38.0	36.0	40.0	31.0	41.0
16	36.768387807297785	38.0	35.0	40.0	31.0	41.0
17	36.69116857348297	38.0	35.0	40.0	31.0	41.0
18	36.41954509461267	38.0	35.0	40.0	30.0	41.0
19	36.361002648608796	38.0	34.0	40.0	30.0	41.0
20	36.5086694153947	38.0	35.0	40.0	30.0	41.0
21	36.619442244855236	38.0	35.0	40.0	31.0	41.0
22	36.59950031401032	38.0	35.0	40.0	31.0	41.0
23	36.54632334871529	38.0	35.0	40.0	31.0	41.0
24	36.16617972312481	38.0	34.0	40.0	30.0	41.0
25	36.311097761880056	38.0	35.0	40.0	30.0	41.0
26	36.2064913669916	38.0	35.0	40.0	30.0	41.0
27	36.079772274253884	38.0	35.0	40.0	30.0	41.0
28	35.94031983544039	38.0	34.0	40.0	29.0	41.0
29	35.68227161437712	38.0	34.0	40.0	29.0	41.0
30	35.60410124784971	38.0	34.0	40.0	28.0	41.0
31	35.501119515058846	38.0	34.0	40.0	27.0	41.0
32	35.33904923135734	38.0	34.0	40.0	27.0	41.0
33	35.36004696502198	38.0	34.0	40.0	27.0	41.0
34	35.150356333451654	38.0	34.0	40.0	26.0	41.0
35	35.162029325833494	38.0	34.0	40.0	26.0	41.0
36	35.017370686908954	38.0	34.0	40.0	25.0	41.0
37	34.81984909301077	38.0	33.0	40.0	25.0	41.0
38	34.79689903430449	38.0	33.0	40.0	25.0	41.0
39	34.65044280006189	37.0	33.0	40.0	24.0	41.0
40	34.43126814661096	37.0	33.0	40.0	23.0	41.0
41	34.43763026877463	37.0	33.0	40.0	23.0	41.0
42	34.408390901892254	37.0	33.0	40.0	23.0	41.0
43	34.42015491175855	37.0	33.0	40.0	23.0	41.0
44	34.290969245192	37.0	33.0	40.0	23.0	41.0
45	34.073551229191125	37.0	33.0	40.0	23.0	41.0
46	33.91935395789531	37.0	33.0	40.0	23.0	41.0
47	33.74496445767232	37.0	33.0	40.0	22.0	41.0
48	33.63305390965604	36.0	32.0	40.0	22.0	41.0
49	33.465062938590506	36.0	32.0	40.0	20.0	41.0
50	33.340728503945606	36.0	32.0	39.0	20.0	40.0
51	31.23667731571235	35.0	28.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	3.0
11	8.0
12	12.0
13	6.0
14	18.0
15	30.0
16	64.0
17	108.0
18	252.0
19	459.0
20	689.0
21	1045.0
22	1468.0
23	1923.0
24	2277.0
25	2658.0
26	2900.0
27	3444.0
28	4086.0
29	5129.0
30	6431.0
31	8137.0
32	10283.0
33	13482.0
34	19134.0
35	21059.0
36	24059.0
37	31440.0
38	37718.0
39	21411.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.83583176328173	36.48754425725182	17.68378705549336	22.992836923973094
2	24.419080905441938	36.40926922061728	17.439860197143872	21.73178967679691
3	20.2887074607032	30.57777899134424	24.283009766176082	24.850503781776478
4	24.881904813914755	32.55558892863319	16.912868962127625	25.649637295324435
5	16.599313728167182	38.10629021835095	18.83060735967379	26.463788693808084
6	18.3805258990252	38.43986929889232	23.403325778882124	19.776279023200356
7	73.06519582411781	15.040184219388545	6.925520392467393	4.96909956402625
8	76.64991944952625	7.54307402452011	4.985937798651121	10.821068727302514
9	72.8531250853289	11.519627920523533	9.741146274199274	5.886100719948302
10	33.14720257761516	34.09514967825319	13.888813040985173	18.868834703146476
11	26.83286459328837	29.253929679891506	24.559247831508433	19.353957895311687
12	26.381872957795196	25.700151999199043	31.311835003504175	16.60614003950159
13	26.283574074579725	28.63410061072732	25.20865758312172	19.873667731571235
14	18.76917055766413	30.897705449216794	31.80560485669297	18.527519136426108
15	18.504309677889122	32.71896531323667	26.606231056986047	22.170493951888158
16	19.627010348687985	28.91670989997178	29.687172905915226	21.769106845425007
17	18.304981386924428	29.151535009875396	27.99606804467138	24.547415558528794
18	21.579335390328485	29.405928878937644	25.67512219097289	23.33961353976099
19	20.211797686335544	34.38777089078812	26.336364215565812	19.064067207310526
20	22.270613184792797	34.188442599823425	22.53501897714551	21.005925238238266
21	20.4507185830398	31.469295251617837	25.40070447532971	22.67928169001265
22	20.191318752332325	31.527091354249155	24.859605530222357	23.42198436319617
23	21.792771391384285	31.092482865958548	21.653514640162374	25.461231102494793
24	18.12522185511837	36.2968626273107	23.78605430103123	21.791861216539697
25	22.284265807461615	28.16718091545386	23.35645177438586	26.192101502698666
26	22.919112761561497	31.324577451328402	21.26486998152345	24.491439805586655
27	19.16964748928269	28.790650683996393	26.428291874869164	25.611409951851748
28	24.738552275892197	29.241642319489575	23.72552767386615	22.29427773075208
29	20.663244409251018	32.91419781740072	22.348888221427334	24.073669551920922
30	24.85186904404336	28.935823571708124	23.36236791087568	22.849939473372835
31	27.536884835576913	30.367983689666783	19.169192401860396	22.925939072895904
32	22.009393004396145	28.398820413401417	21.967980048967405	27.623806533235033
33	25.45167426662662	27.046300594344174	25.03117348842713	22.470851650602082
34	20.569951487680786	29.793208275309684	22.83856228781549	26.798277949194038
35	23.15621330857658	27.565555343181426	27.033558146519947	22.24467320172205
36	23.752377831781484	28.77699806132758	24.687127397172997	22.783496709717937
37	20.88987794555334	31.064267445776334	22.321582976089708	25.72427163258062
38	24.6206846335181	28.258653487334918	26.05056931436529	21.070092564781696
39	20.6054483066197	26.388244181707304	25.355650820522623	27.65065669115037
40	22.586898943287007	26.859259663781415	28.433407057495746	22.12043433543584
41	20.347413738179103	25.614140476385515	30.95186085246976	23.08658493296562
42	24.519200138346577	25.722451282891445	26.395525580464007	23.362822998297972
43	21.999381081105682	25.21229828250007	27.384885636530775	25.40343499986347
44	22.297008255285842	24.589283601379826	26.036916691696476	27.07679145163786
45	26.89248104560886	25.22913651712494	25.767049850276237	22.11133258698996
46	20.392467392986195	23.962628220881232	32.00356788539078	23.64133650074179
47	22.640144171695383	27.457699624097785	25.068945744477517	24.83321045972931
48	21.092846935896386	25.54951806241979	30.485851332040887	22.87178366964294
49	23.052453376293585	23.445648909155448	28.58631643138647	24.915581283164496
50	20.87440497319535	23.15757857084346	27.515495726729107	28.452520729232084
51	20.387461431340963	22.34342717235981	31.538923627228794	25.73018776907044
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2472.0
1	2020.0
2	1568.0
3	1086.5
4	605.0
5	571.5
6	538.0
7	588.0
8	638.0
9	693.0
10	748.0
11	813.0
12	878.0
13	882.5
14	887.0
15	870.0
16	853.0
17	859.5
18	866.0
19	747.5
20	629.0
21	602.0
22	575.0
23	584.0
24	593.0
25	749.0
26	1064.5
27	1224.0
28	1600.5
29	1977.0
30	2225.0
31	2473.0
32	3255.0
33	4037.0
34	4337.5
35	4638.0
36	4901.0
37	5164.0
38	5615.0
39	6066.0
40	6901.0
41	7736.0
42	9266.5
43	10797.0
44	16198.5
45	21600.0
46	22497.5
47	23395.0
48	22401.5
49	21408.0
50	20421.0
51	19434.0
52	17913.5
53	16393.0
54	14701.0
55	13009.0
56	11863.0
57	10717.0
58	9939.5
59	9162.0
60	8471.0
61	7780.0
62	6744.0
63	5708.0
64	4872.5
65	4037.0
66	3400.0
67	2763.0
68	2319.5
69	1876.0
70	1729.5
71	1583.0
72	1465.5
73	1348.0
74	1220.5
75	806.0
76	519.0
77	512.0
78	505.0
79	383.5
80	262.0
81	193.5
82	125.0
83	91.5
84	58.0
85	58.5
86	59.0
87	38.5
88	18.0
89	13.0
90	8.0
91	5.5
92	3.0
93	3.5
94	4.0
95	2.5
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	219738.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.436574465954905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.88509336164367	29.107391529912896
2	7.952288765378131	5.795083235489538
3	2.6353587709985637	2.880703383119897
4	1.3813776306750765	2.0133067562278715
5	0.8405670392805845	1.5313691760187134
6	0.6881908449384875	1.5045190181033776
7	0.5557984137887966	1.4175973204452577
8	0.5033410354087304	1.467201849475284
9	0.4059201898457503	1.3311307102094312
>10	4.876038218947105	37.92061455005506
>50	0.23231124711172174	5.324977928260019
>100	0.03122462998813464	2.2080841729696274
>500	0.006244925997626928	1.3183882623852041
>1k	0.006244925997626928	6.1796321073278175
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCC	3660	1.665619965595391	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGT	3318	1.5099800671709036	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT	2917	1.3274900108310805	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2113	0.9615997233068472	No Hit
CTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGCT	1571	0.7149423404235954	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT	713	0.32447733209549556	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT	565	0.2571243935960098	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	564	0.25666930617371597	No Hit
AAACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT	537	0.24438194577178274	No Hit
AACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTG	518	0.23573528474820013	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	438	0.19932829096469432	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTAT	417	0.18977145509652404	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	383	0.17429848273853407	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT	378	0.17202304562706497	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	315	0.14335253802255413	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	246	0.11195150588428036	No Hit
AATGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC	227	0.10330484486069774	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9379351773475685	0.0
2	0.0	0.0	0.0	1.157287314893191	0.0
3	0.0	0.0	0.0	1.706577833601835	0.0
4	0.0	0.0	0.0	4.554059834894283	0.0
5	0.0	0.0	0.0	4.922225559529986	0.0
6	0.0	0.0	0.0	6.574647989878856	0.0
7	0.0	0.0	0.0	7.819312089852461	0.0
8	0.0	0.0	0.0	8.702181689102476	0.0
9	0.0	0.0	0.0	11.351700661697112	0.0
10	0.0	0.0	0.0	12.884435099982706	0.0
11	0.0	0.0	0.0	16.102358263022328	0.0
12	0.0	0.0	0.0	16.919240186039737	0.0
13	0.0	0.0	0.0	17.338830789394642	0.0
14	0.0	0.0	0.0	17.862636412454833	0.0
15	0.0	0.0	0.0	18.16481446085793	0.0
16	0.0	0.0	0.0	18.610800134705876	0.0
17	0.0	0.0	0.0	19.143707506211943	0.0
18	0.0	0.0	0.0	19.99472098590139	0.0
19	0.0	0.0	0.0	20.27550992545668	0.0
20	0.0	0.0	0.0	20.616370404754754	0.0
21	0.0	0.0	0.0	20.952680009829887	0.0
22	0.0	0.0	0.0	21.286714177793556	0.0
23	0.0	0.0	0.0	21.652604465317786	0.0
24	0.0	0.0	0.0	21.923381481582613	0.0
25	0.0	0.0	0.0	22.139092919749885	0.0
26	0.0	0.0	0.0	22.360720494406976	0.0
27	4.5508742229382265E-4	0.0	0.0	22.597365953999763	0.0
28	4.5508742229382265E-4	0.0	0.0	22.844023336883016	0.0
29	4.5508742229382265E-4	0.0	0.0	23.100237555634436	0.0
30	4.5508742229382265E-4	0.0	0.0	23.462942231202614	0.0
31	4.5508742229382265E-4	0.0	0.0	23.703228390173752	0.0
32	4.5508742229382265E-4	0.0	0.0	23.97582575612775	0.0
33	4.5508742229382265E-4	0.0	0.0	24.26116556990598	0.0
34	4.5508742229382265E-4	0.0	0.0	24.50190681629941	0.0
35	4.5508742229382265E-4	0.0	0.0	24.81455187541527	0.0
36	4.5508742229382265E-4	0.0	0.0	25.04710154820741	0.0
37	4.5508742229382265E-4	0.0	0.0	25.289208056867725	0.0
38	4.5508742229382265E-4	0.0	0.0	25.54587736304144	0.0
39	4.5508742229382265E-4	0.0	0.0	25.87445048193758	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAA	25	3.8804756E-5	45.000004	15
AATCACG	25	3.8804756E-5	45.000004	1
TAGACGG	25	3.8804756E-5	45.000004	2
ATAGCGG	25	3.8804756E-5	45.000004	2
ACGGGTA	30	2.1574633E-6	45.000004	5
ATACTGG	45	3.8198777E-10	45.000004	2
TACGAGG	30	2.1574633E-6	45.000004	2
TCTCGTA	50	2.1827873E-11	45.000004	28
GCCCTAC	30	2.1574633E-6	45.000004	26
GCGATAG	30	2.1574633E-6	45.000004	1
GATTGCG	25	3.8804756E-5	45.000004	9
TATCGGG	25	3.8804756E-5	45.000004	3
GGCCTAT	25	3.8804756E-5	45.000004	8
ACGAAAT	25	3.8804756E-5	45.000004	40
TCGTTGA	20	7.020822E-4	45.0	24
ACGCCCA	20	7.020822E-4	45.0	29
GGTCGAA	20	7.020822E-4	45.0	12
GGGTCGA	20	7.020822E-4	45.0	11
CGTTGAT	20	7.020822E-4	45.0	25
ATCGTTG	20	7.020822E-4	45.0	23
>>END_MODULE